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SAC KY (CHROMATOGRAPHY)
LA PHNG PHAP TACH DA TREN 2 QUA TRNH * HAP PHU * GIAI HAP PHU XAY RA LIEN TUC GIA 2 PHA: * PHA TNH : THNG LA RAN HOAC LONG * PHA ONG : CHAT LONG (MOT CHAT HOAC HON HP NHIEU CHAT)
NH TNH : DA VAO THI GIAN LU NH LNG : DA VAO CHIEU CAO HOAC DIEN TCH PEAK
INJECTOR
STATIONARY PHASE
MOBILE PHASE
CHROMATOGRAPHY
DETECTOR
tM
tR1
tR2 tR3
tR4
Start
COT TACH : LA NI XAY RA CAC QUA CH TRNH TACH CHAT. GOM COT PHAN CH T. TCH VA COT BAO VE
Injector
Column
Mobile phase
Detector
tM
Start
tR1
tR2
tR3
tR4
+ Mot valve mot chieu vao, ch cho phep dung moi vao au bm. Khong cho dung moi ra. + Mot valve mot chieu ra, ch cho phep dung moi ra khoi bm, khong cho dung moi vao. + He thong bm piston chnh xac ieu khien bang mo t bc (step mo t)
DUAL PISTON
-Nhieu bm se lam tang nhieu nen do piston di chuyen se tao xung dong. - Giam nhieu bang hai cach : + Dung dch bm qua mot ong ng knh nho e giam xung. + Thay oi ket cau banh rang, s dung bm kep e piston di chuyen nhe va t nhieu.
HE THONG GRADIENT
GRADIENT TRONG SAC KY LONG LA G ? -LA S THAY OI THANH PHAN PHA ONG TRONG QUA TRNH CHAY THEO NHNG NH HNG CU THE MUC CH : NHAM TANG CNG KHA NANG TACH CUA CAC CHAT TREN COT. NGUYEN NHAN : + NHIEU CHAT KHONG THE TACH C TREN COT TACH VI MOT THANH PHAN PHA ONG (DO TNG TAC CUA CAC CHAT CAN TACH VI PHA ONG VA PHA TNH GIONG NHAU) + NEU THAY OI THANH PHAN PHA ONG : TNG TAC GIA CHAT PHAN TCH VA PHA TNH, PHA ONG SE THAY OI. V VAY SE TACH C CAC CHAT.
HE THONG GRADIENT
-BAO GOM HE GRADIENT AP SUAT THAP VA HE GRADIENT AP SUAT CAO -HE GRADIENT AP SUAT CAO (HIGH PRESSURE GRADIENT: HPGE) CO O CHNH XAC THANH PHAN PHA ONG CAO HN NHNG THIET B AT HN V TOI THIEU CAN 02 BM SAC KY LONG -HE GRADIENT AP SUAT THAP (LOW PRESSURE GRADIENT : LPGE) CO O CHNH XAC THANH PHAN PHA ONG THAP HN NHNG CH CAN MOT BM LC CO THE TRON VA THAY OI 04 DUNG MOI KHAC NHAU.
BO TIEM MAU
-DUNG E A MAU VAO HE THONG -GOM VALVE 6 CONG -C CHE HOAT ONG NH SAU : + VALVE TIEM MAU CO VONG MAU (LOOP) CO THE TCH CO NH (THNG 20 uL)
LOOP
V TR LOAD, THE TCH MAU CAN THIET SE GI TRONG LOOP, PHAN D SE THAI RA NGOAI. IEU NAY GIUP THE TCH MAU TIEM VAO MAY LUON GIONG NHAU
KHI CHUYEN VE V TR INJECT, MAU T LOOP SE C BM VAO HE THONG E VAO COT TACH
AU DO (DETECTOR)
-QUAN TRONG NHAT LA AU DO UV VIS -NGUYEN TAC GIONG NH MAY O UV-VIS THONG THNG -TUY NHIEN CELL O PHAI CO THE TCH NHO (DI 20 uL) E PEAK KHONG B DOAN CHAN VA PHAI CHU C AP SUAT CAO E KHONG B BE
AU DO (DETECTOR)
-TUY MUC CH MA NHIEU AU DO KHAC NHAU C S DUNG : + AU DO HUYNH QUANG E PHAN TCH ALFLATOXIN, MYCOTOXIN, AMINO ACID, THUOC TR SAU HO CARBAMATE + AU DO CH SO KHUC XA (RI) DUNG E O CAC LOAI NG N, NG KEP + AU DO O DAN E XAC NH CAC ION VO C, HU C + AU DO KHOI PHO (MS) E XAC NH PHAN LN CAC HP CHAT HU C + CAC LOAI AU DO KHAC
AU DO O DAN
AU DO IEN HOA
AU DO ELSD
PHAN TCH CAC AXIT TRONG BIA VI HE THONG HPLC CHUYEN DUNG
PHAN TC AXIT BEO TRONG THC PHAM VI AU DO UV-VIS VA HUYNG QUANG (RF)