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BO CO THC HNH BI 14 NH LNG TNG VI KHUN HIU KH TRONG THC PHM BNG PHNG PHP M KHUN LC
I. NGUYN TC Tng s vi khun hiu kh l tng s nhng vi khun thuc nhm vi khun hiu kh tn ti trong mi trng. Tng s cc nhm vi khun ny trong thc phm c th xc nh bng phng php nui cy tri ln b mt thch hoc bng phng php to hp . Thng qua s lng khun lc m c trn cc a peptri cho php xc nh c lng vi sinh vt cn c kh nng sinh trng trong mi trng trong mu ban u. m kt qu chnh xc th s vi khun trong a phi trong gii hn 25-250 khun lc. Nu vt qu gii hn th phi tin hnh pha long v chn nhng pha long ln hn. Ch tiu tng s vi sinh vt hiu kh c dng nh gi cht lng ca mu v vi sinh vt, nguy c h hng, thi hn bo qun ca sn phm, mc v sinh trong qu tr n h c h b i n , b o q u n s n p h m . II. DNG C, MI TRNG, HA CHT Mi trng s dng: Plate count agar (PCA), pH = 7.0 0.2 Casein peptone: 5.0g Cao nm men: 2.5g Dextrose: 1.0g Agar: 15.0g Nc ct 1000ml
Mi trng c nu si cho tan agar, phn phi vo bnh tam gic, hp tit trng 121oC/20 pht, m (45 50oC) phn phi vo cc a petri. Nc mui sinh l 0.9%.
1 TRN THANH TNG MSSV: 10243221| GVHD: Thy TRNG V ANH DNG
Nc ct v trng. Cn 96o. Nc ma. III. TIN HNH TH NGHIM 1. LY MU Quy trnh ly mu c nhng yu cu sau: Ly cc mu c tnh cht i din, lng mu va . Dng c ly mu, cha mu phi v trng. Mu ly xong, phi phn tch ngay, khng c qu 24h. Mu ly phi c nhn ghi k hiu ng thi phi ghi vo s nhng c im ca mu v ni thu mu.
2. PHA LONG MU
TRN THANH TNG MSSV: 10243221| GVHD: Thy TRNG V ANH DNG
3. QUY TRNH
Mu
ng nht
Pha long
Hp
Hp tri
m khun lc ( 25 n 250 )
Tnh kt qu
TRN THANH TNG MSSV: 10243221| GVHD: Thy TRNG V ANH DNG
Hnh 14.2 Quy trnh to hp 4. CCH M CNG THC TNH 4.1. Cch m
C ( n1vd1 ... ni vd i )
TRN THANH TNG MSSV: 10243221| GVHD: Thy TRNG V ANH DNG