THC HNH VI SINH I CNG

BO CO THC HNH BI 20 PHNG PHP PHN TCH SALMONELLA SPP. TRONG THC PHM
I. NGUYN TC 1. SALMONELLA SPP. Salmonella l vi khun thuc h Enterobacteriaceae, c hnh que, Gram m, khng sinh bo t, sng hiu kh ty tin nhng pht trin tt trong iu kin hiu kh. Hu ht chng c kh nng di ng nh tin mao v c kh nng to H2S (tr S. Typhi). Chng c kh nng ln men sinh acid t glucose, mannitol, khng ln men lactose, sucrose, khng to Indol; c kh nng to enzyme lysine decarboxylase. Chng c mt trong nhiu loi thc phm, gy ng c vi mt thp (khong 1 triu t bo/g). Trong hu ht cc loi thc phm, ch s Salmonella lun lun bng 0. 2. NGUYN TC Qu trnh phn tch Samonella gm 4 bc: tng sinh, tng sinh chn lc, phn lp, v khng nh. Bc tng sinh chn lc thng dng cc loi mi trng chuyn bit ty vo ngun mu thu nhn. Bc khng nh nhm xc nhn cc khun lc c trng cho Samonella xut hin trn mi trng phn lp. Bc ny da trn vic s dng cc th nghim sinh ha v th nghim huyt thanh c trng cho Samonella. II. DNG C, MI TRNG, HA CHT Dng c phng th nghim Cc loi mi trng: Dung dch BPW Mi trng SCB Mi trng XLD Mi trng KIA Mi trng SS Agar Mi trng thch Mac Conkey
TRN THANH TNG MSSV: 10243221| Thy: TRNG V ANH DNG

THC HNH VI SINH I CNG

Nc mui sinh l 0.9% Nc ct v trng Mu nguyn liu .... III. TIN HNH TH NGHIM QUY TRNH:
Mu

SPW (saline peptone water) 24h, 37oC

RV

Selenite

XLD

SS Agar

Khng nh (chn 5 khun lc in hnh)

Th phn ng sinh ha

Th nghim ngng kt huyt thanh a gi O, H

TRN THANH TNG MSSV: 10243221| Thy: TRNG V ANH DNG

THC HNH VI SINH I CNG

C KT QU TH NGHIM SINH HA XC NH SALMONELLA Mu c Salmonella khi: Lm c mi trng tng sinh To khun lc c trng trn mi trng phn lp Trc khun Gram m C tnh di ng Lactose (-), Saccharose (-) Dextrose hoc Glucose (+) Dihydrosunfua (+) Urease (-) Indol (-) Manitol (+) VP (-)

IV. NHNG IM CN LU Salmonella l nhm vi sinh vt nguy hi, cn ht sc cn thn khi phn tch. Mang gng tay, khu trang trong qu trnh lm th nghim. Cc mi trng tng sinh chn lc, mi trng phn lp Salmonella c cc thnh phn d bin tnh nhit cao, do , khng c hp tit trng khi pha ch. V. BO CO THC TP Ti sao khi phn tch Salmonella trong thc phm, ch cn phn tch nh tnh Ch tiu Samonella l 1 trong nhng ch tiu rt quan trng trong kim nh cc loi hi sn nh c, mc, tmtheo nhng tiu chun VN quy nh v s c mt ca Samonella trong 25gam cc loi ny l 0, ngha l khng cho php s c mt ca n trong thc phm ny. Nn chng ta ch cn pht hin s c mt ca Samonella khng cn phn tch nh lng. Trnh by quy trnh phn tch Samolnella trong thc phm

TRN THANH TNG MSSV: 10243221| Thy: TRNG V ANH DNG

THC HNH VI SINH I CNG

QUY TRNH:
Mu

SPW (saline peptone water) 24h, 37oC

RV

Selenite

XLD

SS Agar

Khng nh (chn 5 khun lc in hnh)

Th phn ng sinh ha

Th nghim ngng kt huyt thanh a gi O, H

TRN THANH TNG MSSV: 10243221| Thy: TRNG V ANH DNG