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(+) nhanh: mu i trong vng 1-6 gi (+) chm : mu i trong vng 24 gi n 6 ngy hoc lu hn. 3. TH NGHIM GELATINASE MC CH Th nghim kh nng phn gii gelatin bi gelatinase C S SINH HA
gelatinase
Gelatine
Gelatine trong mi trng dinh dng mi trng ng c VSV phn hy gelatine mi trng lng. i chng dng: Aeromonas hydrophila i chng m: E.Coli Mi trng s dng: Nutrient Gelatine Dng ng nghim thch su Cy vi sinh vt v nhit phng. KT QU: c kt qu (+): mi trng tan chy (-) : mi trng khng tan chy
370C/24h
C ch: C ba trng hp xy ra: Ch s dng glucose, sau 18-24h nui cy phn nghing (b mt) tr nn c pH kim v phn ng (phn su trong ng nghim) c pH acid. Do Glucose trn b mt ca mi trng c vi sinh vt oxy ha hon ton thnh CO2 v nc thu ly nng lng, p ng nhu cu nng lng trong tng trng. Vi sinh vt tip tc d ha peptone qua gii phng NH3 lm phn b mt ca mi trng c pH kim. Trong khi , phn sau trong mi trng c iu kin oxy khng y , glucose c ln men k kh sinh cc acid hu c lm pH mi trng gim. S dng c glucose v Lactose, sau 18-24h, ton b mi trng u tr nn c PH acid v s bin dng ng thi c hai loi ng gip vi sinh vt nng lng tng trng m cha cn s dng n peptone. Nu ko di thi gian nui cy qu 24h b mt pH s tr nn kim do ht ngun cacbon v vi sinh vt pha s dng n peptone. Khng s dng glucose, lactose: vi sinh vt s bin dng peptone thu ly nng lng v vt cht cho s tng trng. tuy nhin do peptone ch c bin dng trong iu kin hiu kh nn hin tng kim ha ca mi trng ch din ra trn b mt mi trng. Kh nng sinh H2S do trong mi trng sodium thiosunfat, vi sinh vt kh sunface c th kh cht ny, nh c enzyme thiosunfat reductase gii phng H2S, H2S s phn ng vi Ion Fe2+ ca ch th amonium citrate to kt ta mu en F2S.
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ngha: nh danh cc loi vi khun c kh nng: s dng cc ngun cacbon khc nhau v c kh nng sinh H2S, kh nng sinh hi. Thng c dng nh danh cc vi khun gram (-), vi sinh ng rut. 6. TH NGHIM KH NNG SINH H2S MC CH: Pht hin kh nng sinh H2S ca vi sinh vt. C S SINH HA:
desulfohydrase
H2 S
Thiosulfate
H2 S
H2S sinh ra c nhn bit bi ion st, ch to kt ta mu en (FeS, PbS). MI TRNG: KIA, TSA (thch nghing) SIM, PIA (thch su) BSA (thch a) Cy vi sinh vt ln mi trng, 37oC, 24 - 48h
TRN THANH TNG MSSV: 10243221| Thy: TRNG V ANH DNG
7. TH NGHIM CATALASE MC CH: Pht hin vi sinh vt c h enzyme catalase C S SINH HA: Catalase hin din cc VSV hiu kh v k kh ty
Catalase
VSV ly t mi trng nui cy (lng, rn) t VSV ln lam knh sch Nh H2O2 30% Quan st sau 1-2 giy Phn ng (+): c bt kh xut hin
Th nghim catalase trn a petri: s dng H2O2 30% 8. KH NNG LN MEN CC LOI NG
7 TRN THANH TNG MSSV: 10243221| Thy: TRNG V ANH DNG
MI TRNG: Phenol Red Broth Base THC HIN: Cy vi khun mi hot ho vo cc ng nghim, t 36 0C, theo di hin tng sinh acid sau 1-3 ngy. - Lc v trng mi trng qua mng lc c kch thc l 0,45m hoc 0,2m. - Phn phi vo cc ng nghim . Hp 1210C trong 15 pht - Tin hnh cy chng vo cc ng mi trng bng que cy vng. - cc ng mi trng 370C trong 18 20 gi. Trong trng hp khng nh kt qu m tnh, cn dng paraffin bt kn nt bng v theo di thi gian c th ko di n trong 14-30 ngy.
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