THC HNH VI SINH I CNG

BO CO THC HNH BI 2: PHNG PHP PHA CH MI TRNG NUI CY VI SINH VT

I. NGUYN TC 1. NH NGHA Cc cht dinh dng l nhng hp cht tham gia vo qu trnh trao i cht ni bo. Mi trng dinh dng l hn hp gm cc cht dinh dng v cc cht c nhim v duy tr th oxy ha kh, p sut thm thu ca t bo v s n nh pH ca mi trng. 2. YU CU .... 3. PHN LOI 3.1. Phn loi theo ngun gc: Mi trng t nhin: c thnh phn l cc sn phm t nhin nh dch nc chit tht, nc chit khoai ty, mu ng vt, ....Thnh phn ha hc ca loi mi trng ny khng c xc nh chnh xc do s khng n nh ca sn phm t nhin. Mi trng nhn to: cha cc cht ha hc m thnh phn ca chng c xc nh v nh lng mt cch c th v chnh xc nh Czapeck, Hansen, EMB, ... Mi trng bn t nhin: cha cc cht ha hc ln cc sn phm t nhin nh Potato glucose agar (PGA), ... 3.2. Phn loi theo trng thi vt l:
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C cc cht dinh dng cn thit; C pH thch hp; C nht nht nh; Khng cha cc yu t c hi; Hon ton v trng m bo s pht trin n nh ca vi sinh vt;

THC HNH VI SINH I CNG

Mi trng lng: dng lng, khng c agar hay cc cht lm gi th khc trong thnh phn mi trng, thng c s dng nghin cu qu trnh tng hp ca vi sinh vt. Mi trng rn: mi 1000 ml mi trng c 15 20g agar hay cc cht lm gi th, c s dng nghin cu cc c im hnh thi, sinh l ca vi sinh vt. Mi trng bn lng ( bn rn ): mi 1000 ml mi trng c 3 5g agar hay cc cht lm gi th khc. 3.3. Phn loi theo cng dng: Mi trng phn lp Mi trng tng sinh Mi trng lu gi ging Mi trng th nghim sinh ha ...

Mi trng nui cy vi sinh vt c pha ch theo nguyn tc: Da trn c s nhu cu v cc cht dinh dng v kh nng ng ha cc cht dinh dng ca tng loi sinh vt. m bo s cn bng v p sut thm thu gia mi trng v t bo vi sinh vt nn cn iu chnh t l v nng cc cht trong thnh phn mi trng. m bo cc iu kin ha l cn thit cho cc hot ng trao i cht ca vi sinh vt. II. QUY TRNH THC HIN 1. MI TRNG C QUA HP V TRNG

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THC HNH VI SINH I CNG

Cn, ong thnh phn mi trng

nh mc

Rn un tan Lng

iu chnh pH

Erlen Phn phi vo dng c cha ng Nghim Hp v trng Mi trng lng MT thch nghing MT thch ng

To hnh mi trng

Kim tra v trng

Bo qun

S dng

Trn Thanh Tng | MSSV: 10243221

THC HNH VI SINH I CNG

2. MI TRNG KHNG QUA HP V TRNG

V trng dng c cha, dng c, thit b cn

Cn ha cht trong iu kin v trng ( cn, tia UV ) Erlen Phn phi vo dng c cha ng Nghim Rn un tan Lng

Mi trng lng MT thch nghing MT thch ng

To hnh mi trng

Kim tra v trng

Bo qun

S dng

Trn Thanh Tng | MSSV: 10243221

THC HNH VI SINH I CNG

III. MI TRNG, DNG C, HA CHT 1. Mi trng SABOURAUD (100ml) ( Mi trng nui cy nm men, pH = 7 0.2 ) Glucose: 20g Agar: 15g NaCl: 3g 2. Mi trng Cao tht Peptone ( 100ml) ( Mi trng nui cy vi khun, pH = 7 0.2 ) Cao tht: 3g Peptone: 10g NaCl: 5g Agar: 15g ...... IV. BO CO THC TP Trnh by khi nim mi trng v phn loi mi trng nui cy vi sinh vt KHI NIM: Mi trng nui cy l cc c cht dinh dng c pha ch nhn to nhm p ng cho nhu cu sinh trng, pht trin v sn sinh cc sn phm trao i cht ca vi sinh vt. Mi trng dinh dng dng trong nghin cu vi sinh vt v trong qu trnh sn xut cc sn phm ca vi sinh vt. Mi trng dinh dng l yu t quan trng trong cng nghip ln men, cng nghip sinh tng hp nh vi sinh vt. PHN LOI: 1. Phn loi theo ngun gc: Mi trng t nhin: c thnh phn l cc sn phm t nhin nh dch nc chit tht, nc chit khoai ty, mu ng vt, ....Thnh phn ha hc ca loi mi trng khng c xc nh chnh xc do s khng n nh ca sn phm t nhin.
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THC HNH VI SINH I CNG

Mi trng nhn to:cha cc cht ha hc m thnh phn ca chng c xc nh v nh lng mt cch c th v chnh xc nh Czapeck, Hansen, EMB, ... Mi trng bn t nhin: cha cc cht ha hc ln cc sn phm t nhin nh Potato glucose agar (PGA), ... 2. Phn loi theo trng thi vt l: Mi trng lng: dng lng, khng c agar hay cc cht lm gi th khc trong thnh phn mi trng, thng c s dng nghin cu qu trnh tng hp ca vi sinh vt. Mi trng rn: mi 1000 ml mi trng c 15 20g agar hay cc cht lm gi th, c s dng nghin cu cc c im hnh thi, sinh l ca vi sinh vt. Mi trng bn lng ( bn rn ): mi 1000 ml mi trng c 3 5g agar hay cc cht lm gi th khc. 3. Phn loi theo cng dng: Mi trng phn lp Mi trng tng sinh Mi trng lu gi ging Mi trng th nghim sinh ha ...

Yu cu ca mi trng trong a petri, ng nghim thch nghing v thch ng A PETRI: Mi trng ch c phn phi vo a sau khi hp tit trng. Th tch mi trng khong 12 15ml mi a, lp mi trng thch dy khong 2mm. NG NGHIM THCH NGHING: Lng mi trng phn phi vo chim 1/4 th tch ca ng nghim. Sau khi phn phi mi trng vo ng nghim, ng nghim c t nghing c nh trn gi . Phn nh nghing phi cch nt y 3 5 cm. Phn y phi c mt phn thch ng 0.5 1 cm. NG NGHIM THCH NG: Lng mi trng phn phi vo chim 1/3 1/2 th tch ca ng nghim. Sau yn trn gi cho n khi mi trng ngui v ng c. Gii thch ti sao khng phn phi mi trng vo a petri trc khi kh trng? Khng phn phi mi trng vo a petri trc khi kh trng v:

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THC HNH VI SINH I CNG

Do chiu cao ca a petri rt thp, nn khi ta cho mi trng vo mi tin hnh hp kh trng, mi trng trong a s b tro ra ngoi, dn n mi trng b c, sau ny s dng kh quan st c vi sinh vt. Bn cnh , khi mi trng b tro ra ngoi khi hp s nh hng n cht lng mi trng, mi trng sau ny d nhim nhng vi sinh vt khng mong mun, lng mi trng trong a cng gim i, nh hng n qu trnh nui cy, quan st sau ny. a petri cha mi trng trc khi nui cy vi sinh vt nn t p hay nga? Ti sao? a petri cha mi trng trc khi nui cy vi sinh vt nn t p.V: Trong qu trnh s dng d cm nm, trnh ri rt a; Khi t p, a petri v np a kn hn khi nga, trnh c hi nc do trnh c tp nhim nhng vi sinh vt khng mong mun. Trnh by quy trnh pha ch mi trng nui cy vi sinh vt

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THC HNH VI SINH I CNG

MI TRNG C QUA HP V TRNG

Cn, ong thnh phn mi trng

nh mc

Rn un tan Lng

iu chnh pH

Erlen Phn phi vo dng c cha ng Nghim Hp v trng Mi trng lng MT thch nghing MT thch ng

To hnh mi trng

Kim tra v trng

Bo qun

S dng Trn Thanh Tng | MSSV: 10243221

THC HNH VI SINH I CNG

MI TRNG KHNG QUA HP V TRNG

V trng dng c cha, dng c, thit b cn

Cn ha cht trong iu kin v trng ( cn, tia UV ) Erlen Phn phi vo dng c cha ng Nghim Rn un tan Lng

Mi trng lng MT thch nghing MT thch ng

To hnh mi trng

Kim tra v trng

Bo qun

S dng

Trn Thanh Tng | MSSV: 10243221