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8—1996
ISO 9888:1991
Australian Standard
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Biodegradability—Organic
compounds in an aqueous medium
Griffith University
Review of Australian Standards. To keep abreast of progress in industry, Australian Standards are subject to
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Australian Standard
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Biodegradability—Organic
compounds in an aqueous medium
PREFACE
This Standard was prepared by the Standards Australia Committee MS/56 on Biodegradability
of Materials. It is technically equivalent to ISO 9888:1991, Water quality—Evaluation of the
aerobic biodegradability of organic compounds in an aqueous medium —Static test (Zahn-
Wellens method) .
This is Part 8 in a series of Standards which give guidance and methods to determine the
biodegradability of organic compounds in an aqueous medium with the objective of
specifying a method for the determination of the ‘inherent’ biodegradability of an organic
compound at a given concentration by aerobic microorganisms. Determination of the
percentage removal of dissolved organic carbon (DOC) or chemical oxygen demand (COD)
at regular intervals allows evaluation of the biodegradability of the test compound.
The term ‘informative’ has been used in this Standard to define the application of the
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appendix to which it applies. An ‘informative’ appendix is only for information and guidance.
CONTENTS
Page
1 SCOPE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
2 REFERENCED DOCUMENTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
3 DEFINITIONS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
4 PRINCIPLE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
5 TEST ENVIRONMENT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
6 REAGENTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
7 APPARATUS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
8 PROCEDURE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
9 CALCULATION AND EXPRESSION OF RESULTS . . . . . . . . . . . . . . . . . . . . 8
10 VALIDITY OF THE TEST . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
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11 TEST REPORT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
STANDARDS AUSTRALIA
Australian Standard
Biodegradability— Organic compounds in an aqueous medium
1 SCOPE This Standard specifies a method for the evaluation of the elimination and
the ‘inherent’ biodegradability of organic compounds, at a given concentration, by aerobic
microorganisms.
The conditions described in this Standard normally correspond to optimal conditions for
allowing the maximum value of biodegradation to occur with the chosen inoculum in the
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test time.
The method applies to organic compounds which are —
(a) soluble at the concentration used under the test conditions;
(b) non-volatile, or which have a negligible vapour pressure under the test conditions;
(c) not lost by foaming from the test solution; and
(d) not inhibitory to the test microorganisms at the concentration chosen for the test.
Inhibitory effects can be determined by using a suitable test method (e.g. see
ISO 8192). If the test compound is toxic, the test concentration has to be lower or a
pre-exposed inoculum can be used.
The method can also be used to measure the elimination and biodegradation of organic
compounds in waste water (also called ‘test compound’ in the method).
WARNING AND SAFETY PRECAUTIONS: ACTIVATED SLUDGE AND
SEWAGE, MAY CONTAIN POTENTIALLY PATHOGENIC ORGANISMS.
THEREFORE, APPROPRIATE PRECAUTIONS SHOULD BE TAKEN WHEN
HANDLING THEM. TOXIC TEST COMPOUNDS AND THOSE WITH
UNKNOWN PROPERTIES SHOULD BE HANDLED WITH CARE.
3 DEFINITIONS For the purpose of this Standard, the definitions below apply.
3.1 Concentration suspended solids (of an activated sludge) — the amount of solids
obtained by filtration or centrifugation of a known volume of sludge under specified
conditions and drying at 105°C to constant weight.
COPYRIGHT
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