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vn
MT S PHNG PHP TA PROTEIN
I. Phng php ta bng cc tc nhn gy ta l dung mi hu c (Ethanol 80%,

Aceton) ha tan ca protein trong dung dch ph thuc vo nhiu yu t, mt trong s l hng s in mi ca dung dch. Nhn chung, nhng phn t dung mi c hng s in mi ln (nh nc, dimethylsulphoxid) c th n nh tng tc gia chng vi cc phn t protein v to iu kin thun li cho s ha tan ca protein trong dung dch. Ngc li, cc dung mi vi hng s in mi nh (aceton, ethanol) ngn cn s phn tn ca cc phn t protein trong mi trng. Do , ha tan ca nhng phn t protein gim v xy ra kt ta do s lm gim hng s in mi hin hu ca mi trng. iu ny c c bng cch thm mt dung mi ha tan trong nc nh aceton vo dung dch cha protein. S ta bng aceton hoc ethanol 80% c nhiu thun li hn v n tng i r, c sn dng tinh khit vi t cht tp nhim gy c hay c ch i vi enzym, do nhit bay hi ca dung mi thp nn d tch b dung mi khi ch phm enzym bng phng php sy nh bng qut gi. II. Phng php ta bng mui Ngi ta c th dng mui (NH4)2SO4, NaCl ta protein v cc mui ny va lm trung ha in (do cc ion tc ng tng h vi cc nhm tch in tri du), va loi b lp v hydrat ca phn t protein. Cc protein khc nhau c th b kt ta vi nng mui khc nhau, v vy c th dng mui tch ring cc protein ra khi hn hp ca chng. Phn th nghim 1. Dng c v ha cht Dng c Ha cht Pipet 5ml, 10ml Nguyn liu thm (da) Becher 50ml, 100ml, 250ml Etanol 96% Bnh tia Mui (NH4)2SO4 My ly tm 2. Thc hnh Cn khong 80g n 100g thm, gi nt vt ly nc ri ly tm 6000 vng/pht trong 15 pht ri ong xem th tch ca nc thm l bao nhiu, ghi nhn th tch V. Sau chia i th tch V nc thm va thu c thnh hai phn bng nhau ri tin hnh ta protein bng hai tc nhn ta khc nhau: Ethanol 80% v mui (NH4)2SO4 Quy trnh kt ta enzym Bromelin bng cn 80% Chi ngn, v qu

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Lm sch

Nghin p, xay nhuyn Lc

Ly tm 6000 v/p, trong 15 pht cn 80%, lm lnh 40C Dch trong

Ta cn 4oC / 2 gi

Ly tm 6000 v/p, trong 20 pht B dch trong

Thu ta

Sy kh

Bromelin Lu : Phi trn cn vi dch da theo t l 4:1 (1 da : 4 cn)

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Quy trnh thu nhn enzym Bromelin bng mui (NH4)2SO4 Chi ngn, v qu Lm sch

Nghin p, xay nhuyn Lc

Ly tm 6000 v/p, 15 20pht Ammonium sulfate 70% bo ha Dch trong

Hn hp dch da, nhit phng 30 pht Ly tm 6000 v/p, trong 15 pht Thu ta B dch trong

Sy kh Bromelin Lu : c mui(NH4)2SO4 bo ha 70% ta phi trn vi t l 47,2g mui trong 100ml nc thm. 3. Kt qu: Hy tnh hm lng protein c trong 100g nguyn liu ban u.
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III. Phng php ta protein bng im ng in. 1. Nguyn tc Gi tr pH m ti phn t protein trung ha in gi l im ng in ca protein (pI), gi tr pH = pI phn t protein trung ha in s khng chuyn dch trong in trng, phn t protein s km bn nht d kt ta. Ngi ta li dng tnh cht ny kt ta protein. 2. Dng c v ha cht Dng c Ha cht My khut t Dung dch NaOH 20% My ly tm Dung dch HCl 10% Cn phn tch Ete Bercher 100ml, 250ml Etanol Pipet 5ml, 10ml Giy pH 3. Thc hnh Tch chit protein c: Cn 100g c xay nhuyn trong mt bercher ri cho 4 ln nc. (1 c : 4 nc, g/l) Khuy u ri cho t t NaOH 20% va cho va khuy cho n pH 11 th bng giy pH, khuy khong 45 pht ri ly tm, gi li dch chit trong mt bercher 250ml, b cn li thm nc chit tip ln hai ri ly tm ly dch b b. Dch thu c cho vo bercher 250ml gp chung vi phn dch chit bn trn. Cho t t HCl 100% vo dch chit va cho va khuy n pH thch hp l 6 s thy kt ta, ly tm 4000 vng/pht trong 30 pht, ri b dch thu ta protein, ra ta vi ete : ethanol vi t l 2:1, ta protein sch khi ra ta vi ln vi ete : ethanol. Cn lng ta protein thu c (mg ta) tnh ra hiu sut protein c trong 100g c.

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KHO ST HOT TNH ENZYM BROMELIN
(Xc nh hot tnh enzym Bromelin bng phng php Anson ci tin) 1. Nguyn tc Phng php ny da trn s thy phn protein casein bng enzym Bromelin c trong dch nghin cu, tip lm v hot enzym v kt ta protein cha b thy phn bng dung dch acid trichloracetic. nh lng sn phm c to thnh trong phn ng thy phn bng phn ng mu vi thuc th Folin. Da vo th chun ca Tyrosin tnh lng sn phm do enzym xc tc to nn. 2. Dng c v ha cht Dng c Ha cht ng nghim Dung dch Casein 1% Pipet 1ml, 2ml, 3ml, 5ml, 10ml Dung dch TCA 5% My quang ph Dung dch NaOH 0,5N Becher 50ml, 250ml, 500ml Thuc th Folin Bnh tia Dung dch HCl 0,2N Dung dch Tyrosin chun 1 mM/l trong dung dch HCl 0,2N 3. Chun b ng chun Tyrosin Cc bc dng ng chun Tyrosin Dung dch ha cht 1 2 ng nghim 3 4 5 0,8 0,8 4,2 10 3 6 1,0 1,0 4,0 10 3

Dung dch Tyrosin chun (ml) 0 0,2 0,4 0,6 Lng Tyrosin tng ng (M) 0 0,2 0,4 0,6 Dung dch HCl 0,2N (ml) 5,0 4,8 4,6 4,4 Dung dch NaOH 0,5N (ml) 10 10 10 10 Thuc th Folin (ml) 3 3 3 3 Lc mnh, sau 10 pht o OD bc sng 660nm

ng s 1 l ng th khng (TK), cc ng cn li l ng th nghim (TN). V ng chun Tyrosin tng quan gia lng Tyrosin (M) v OD (OD = ODTN ODTK).

4. Phng php tin hnh

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Ly 6 ng nghim sch, kh, tin hnh lm 3 ng th tht, 3 ng th khng Cc bc chun b mu enzym o hot tnh ng nghim Th tht Th khng Dung dch Casein 1% (ml) 5 5 Dung dch TCA 5% (ml) 0 10 Dung dch enzym mu (ml) 1 1 o Lc u v gi 35,5 C trong 20 pht Dung dch TCA 5% (ml) 10 0 yn 30 pht, lc ly dch trong Dung dch ha cht Ly 2 ng nghim mi khc, cho vo ng th nht 5ml dch lc ca ng th tht v cho vo ng th hai 5ml dch lc ca ng th khng. Tip tc thm vo mi ng 10ml NaOH 0,5N v 3ml thuc th Folin, lc mnh, sau 10 pht o OD bc sng 660nm. Tnh OD = ODTT ODTK, sau da vo th chun suy ra c s M Tyrosin. 5. Tnh kt qu nh ngha n v Anson: mt n v Anson l lng enzym ti thiu trong iu kin th nghim (35,50C: pH 7,6 ) thy phn Casein trong 1 pht to thnh sn phm ha tan trong TCA, phn ng vi thuc th Folin cho ta hp thu OD bc sng 660nm tng ng vi 1M Tyrosin trong ng chun. M Tyro sin* V * L (UI ) H Protease =
t *m *v

Vi: H Protease : hot enzym protease (UI/g) V : tng th tch hn hp trong ng th tht hoc ng th khng (ml) v : th tch dch lc em phn tch (ml) t : thi gian thy phn (pht) m : khi lng mu enzym em i xc nh hot tnh (g) L: pha long enzym M Tyrosin: lng M Tyrosin trong v (ml) suy ra t ng chun

KHO ST HOT TNH INVERTASE

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I. Nguyn tc: Invertase l mt enzym thy gii saccharose thnh glucose v fructose. Trong bi ny ta dng enzym invertase trch trong men bia cho thy gii saccharose, t tnh c hot tnh ca enzym ny. II. Dng c v thuc th Dng c Ha cht Becher 100ml 250ml dung dch saccharose 10% Erlen 250ml dung dch NaOH N/10 ng nghim 50ml Dung dch Iod N/10 Pipet 5ml 10ml Dung dch H2SO4 N Buret 25ml H tinh bt 1% Phu lc Dung dch Na2S2O3 N/20 III. Cch tin hnh iu ch dung dch invertase: cn 0,5g men bia ha tan trong 50ml nc ct. Khuy u trong 5 pht. lng em lc ly dch lc. Thc hin ba ng nghim nh sau: S ng ml dung dch saccharose 10% ml nc ct ml enzym invertase ml enzym invertase un si 1 10 10 2 10 10 10 3 10

thy gii trong 30 pht nhit phng. Tip , em tt c cc ng un cch thy trong 2 pht, lm lnh di vi nc. Ht 5 ml ca hn hp cho vo mt erlen 250ml v nh phn ng glucose nh sau: cho thm vo bnh 10ml dung dch Iod N/10 v 15 ml dung dch NaOH N/10. Thi gian nh NaOH phi ko di khong 2 pht (nh tng git). yn trong bng ti 15 -20 pht. Ly ra thm vo 2ml dung dch H2SO4 N. nh phn lng iod thm cn tha bng dung dch Na2S2O3 N/20 (dng h tinh bt cho thm vo lc cui phn ng lm cht ch th mu).

IV. Kt qu Hot tnh ca invertase c biu th bng s mol saccharose thy gii bi lng enzym invertase c trong 1g men bia trong 1 pht nhit phng th nghim.
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Gi V1 v V2 l s ml Na2S2O3 N/20 dng nh phn ng s 1 v s 2. C nhn xt g v kt qu nh phn trong ng s 3? Hot tnh invertase c tnh theo cng thc sau:

(V1 V2 ) * A * B mol / g / pht 10 20 2 a b * t * m

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a: th tch ly ra trong mi ng nh ng glucose (ml) A: th tch tng cng trong mi ng (ml) b: th tch dung dch enzym cho vo mi ng (ml) B: tng th tch dung dch enzym c c t mg men (ml) t: thi gian thy gii tnh bng pht.

XC NH HOT TNH ENZYM ALPHA AMYLASE (THEO PHNG PHP SMITH & ROE)
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I. Nguyn tc: Hot tnh - amylase biu th kh nng ca enzym amylase xc tc phn ng thy phn tinh bt n dextrin trong 1 pht 500C v c th hin bng s n v ca enzym trong 1g mu. Khi phn ng thy phn tinh bt xy ra, lng tinh bt cn li cha c thy phn hy s to phn ng mu vi iod v c so mu bng my so mu quang hc bc sng 620 nm. II. Dng c v ha cht Dng c Ha cht Bnh nh mc 50ml, 100ml, 250ml Cn 960 Bp n nhit Dung dch tinh bt 1% trong m pH 5,6 Pipet 1ml, 2ml, 3ml, 5ml, 10ml Dung dch HCl 1N Becher 50ml, 100ml, 250ml Dung dch NaCl 3% My quang ph Thuc th Lugol III. Thc hnh 1. Ly trch Amylase Amylase l 1 tp cht protein khng tan trong ru mnh. Cn 10g la ny mm cho vo ci vi 30ml nc ct, gi nt la di nc. yn trong 15 pht, em lc. Ly tt c nc qua lc, thm 4 ln th tch cn 960. Khuy u, ta s thy amylase v cc protein khc ta di dng ni ln. lng, ly tm ly ta. Ha tan ta trong 20ml nc ct. Ta c dung dch amylase kh tinh sch. c bit dung dch ny khng c cht ng kh (th 1ml dung dch thuc th Fehling, un cch thy 3 pht, ta khng c kt ta xut hin)

2. Kho st hot tnh -amylase

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Ly su ng nghim chia thnh 2 l, mi l 3 ng nghim gm th tht v th khng ly trung bnh. Tin hnh th nghim theo bng sau: ng nghim ng th tht (t) ng th khng (o) Nc ct (ml) 0 1 Dch chit - amylase 1 0 Dung dch m (ml) 1 1 Dung dch tinh bt 1% (ml) 1 1 Dung dch NaCl 3% (ml) 0,5 0,5 Dung dch HCl 1N (ml) 0 1 0 em 50 C trong 30 pht Dung dch HCl 1N (ml) 1 0 Nc ct (ml) 5,5 5,5 Thuc th Lugol (ml) 0,5 0,5 Dung dch ha cht Lc u hn hp trong ng nghim ri mang i so mu bc sng 620nm IV. Kt qu: (OD 0 ODt ) * C * L (UI ) H A= OD 0 * t Vi OD0: mt quang ca ng th khng ODt: mt quang ca ng th tht C: lng tinh bt ban u tham gia phn ng (mg) T: thi gian phn ng (pht) L: h s pha long mu enzym.

XC NH M TNG S THEO PHNG PHP KJELDAHL

I. V c ha:
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Nguyn tc: S v c ha cht m l s bin i tt c cc cht m d nm di dng no (hu c, v c, protein) thnh hp cht v c l amonium sulfat (NH4)2SO4 bng cch un si vi H2SO4 m c v cht xc tc.
H2SO4 m c Cht m Xc tc, t0 (NH4)2SO4

Thc hnh: ht chnh xc 1ml nu l nguyn liu lng hay cn chnh xc 1g nu l nguyn liu kh nghin nhuyn cho ng nht, cho vo mt bnh c cao c dung tch t 60ml n 100ml (bnh kjeldahl). Thm vo 5ml H2SO4 m c v khong 0,5g cht xc tc. un si hn hp trong t ht kh c t 2 n 3 gi cho n khi dung dch tr thnh trong sut v c mu xanh do tri nht. ngui v pha long thnh 100ml vi nc. Cht xc tc dng y l hn hp xay nhuyn ca: K2SO4 v CuSO4 c t l theo trng lng l 9: 1 Thc hin 3 s th tht (c cht m) v 3 s th khng c cht m (thay cht m bng nc ct) II. Phng php kjeldahl:

Nguyn tc: Cht m c v c ha nm di dng Amonium sulfat em cho tc dng vi cht kim mnh nh NaOH s phng thch ra amoniac
(NH4)2SO4 + 2NaOH 2NH4OH + Na2SO4

Sau lng amoniac c hi nc ni cun bng mt dng c my Parnas Wargner v c dn n mt bnh tam gic c cha mt lng tha H 2SO4. T y cho php chng ta xc nh c lng amoniac phng thch ra, c ngha l xc nh c lng m c trong mu nguyn liu.

Dng c v ha cht: Dng c: My ct m Parnas Wargner Bnh kjeldahl Bp un

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-

Bnh xt nc ct Becher 250 ml, 100ml, 50ml Erlen 250ml Bnh nh mc 100ml, 50ml Buret 25ml Pipet 1ml, 5ml, 10ml, 20ml. ng ong 25ml

Ha cht: Dung dch NaOH m c (10N)

Dung dch H2SO4 N/100 Dung dch NaOH N/100 (dung dch ny sau khi pha xong dng ngy th ng nng , nhng lu s b carbonat ha nn nng khng cn ng nh ban u, v th phi xc nh li h s chnh l x bng cch nh phn n vi dung dch acid chun (acid oxalic, acid phtalic) Dung dch metil 0,5% pha trong ru etylic.
-

Cch thc hnh: un si bnh nc my Parnas Wargner. t erlen c cha 25ml H2SO4N/100 v vi git metil vo phn ui ca my sao cho phn nhn nhng chm vo dung dch. Ht 10ml dung dch v c ha pha long thnh 100ml, em vo my Parnas Wargner; thm vo 10ml NaOH m c ( ghi nh l khi cho mu cng nh NaOH m c vo my, phi cho xung t t bng kp Mohr, nu cho chy xung qu nhanh th dung dch s b my ht ra ngoi). Tip tc un si bnh nc v cho hi nc li cun amoniac sang erlen trong 3 pht, h erlen xung v tip tc un thm 2 pht na, trng vi ca my bng mt tia nc ct, tt c u cho chy vo erlen ng H2SO4 N/100. Ly erlen ra v nh phn H2SO4 tha bng dung dch NaOH c chun l x N/100 cho u. Khi dung dch i mu (t mu hng nht sang mu da cam): thc hin 3 s th thut v 3 s th khng ly tr s trung bnh. Cch tnh: Nu nguyn liu l cht lng, thng thng lng m c tnh bng s gam m trong 1 lt, nu nguyn liu l cht rn, th trc ht phi tnh m, sau lng m c tnh bng phn trm (s gam m c trong 100g nguyn cht liu) theo kh tuyt i) Gi s nguyn liu l cht lng. Ly 1ml nguyn liu em v c ha v pha long thnh 100ml, sau ht 10ml em chng ct bng my Parnas Wargner. Gi V0 l th tch dung dch NaOH x N/100 (tr s trung bnh ca 3 ln th khng). Gi Vt th tch dng dch NaOH x N/100 (tr s trung bnh ca 3 ln th tht). Vy:
V = V0 Vt

l lng NaOH tng ng vi lng amoniac phng thch bi 10ml dung dch v c ha pha long.
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1mol amoniac tng ng vi 1mol NaOH. Do s mol amoniac phng thch bi 10ml dung dch v c ha pha long l:
CM = Vx(1 / 100) mol = Vx10 5 mol 1000 14.V .x.10 5 g

S gam m c trong 10ml dung dch v c ha pha long l: S gam m c trong 1000gl dung dch v c ha pha long hay trong 1ml nguyn liu
14.Vx.10 5.100 = 14.Vx10 4 g 10

S gam m c trong 1 lt nguyn liu:

14.V .x.10 4 .1000 g / lt = 1,4.V .xg / lt

MT S PHNG PHP NH TNH CHT NG

I. Phn ng mu tng qut (phn ng Molish)

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Tt c cc cht ng u cho mu tm i vi dung dch Naphthol trong H2SO4 m c. trong ng nghim 1 ml dung dch ng 1% v 2 git dung dch Naphthol 15% trong ru. Lc u, nghing ng v cho H2SO4 m c t t theo thnh ng. C s to thnh mt lp mu tm hin ra mt ngn cch 2 cht lng. II. Phn bit gia monosaccharid v disaccharid (phn ng Barfoed) Kh nng kh ca nhng monosaccharid c th ln hn nhiu kh nng kh ca disaccharid cho nn, thay v s kh mi trng base, s kh mi trng acid yu c th gip ta phn bit c monosaccharid v disaccharid. Trong ng nghim ng 2ml thuc th Barfoed, ta thm vo 1ml dung dch ng mun kho st. Ta thc hin ng thi ba s th chng vi cc dung dch glucose 1% , lactose 1% v nc ct. un si cch thy trong 5 pht. Lm lnh, quan st ng c trm hin . un tip tc 8 pht, quan st ng c trm hin . Gii thch hin tng. III. Phn ng vi thuc kh Fehling Thuc kh ny l dung dch CuSO4 vi dung dch soude m c v tartrat kp natrium v kalium. Tc dng ca dung dch soude m c ln CuSO4 cho hydroxyd ng (CuO, H2O) tan trong dung dch nh s hin din ca tartrat. Trong mt ng nghim, 1ml thuc th Fehling v 1ml dung dch glucose. un si cch thy trong 5 pht c trm hin gch Cu2O nh s kh ng nh thnh ng nht bi glucose.

KHO ST LIPID
I. Kho st tnh ha tan ca lipid
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1. Nguyn tc: Lipid khng ha tan trong nc do c sc cng b mt rt cao. Nu dng lc c hc tc ng vo hn hp lipid nc th chng phn tn thnh tng ht nh, nhng ngng tc dng chng li phn thnh 2 lp: lipid trn v nc di. Nu ta thm vo hn hp cc cht mui kim, x phng, mt.. s c tc dng lm gim sc cng b mt ca cc ht lipid do vic tr li hai lp kh hn v dung dch lc ny s c mu trng c nh sa (nh tng), lipid di dng cc ht nh liti cc micell y l hin tng nh ha m. Nu thay nc bng cc dung mi hu c: acetone, ether, chloroform, benzen th lipid s ha tan vo cc dung mi ni trn. 2. Ha cht Du thc vt hay m ng vt Dung mi hu c: alcohol, acetone, bezen 3. Cch lm Cho vo ba ng nghim: ng 1: 2ml alcohol ng 2: 2ml acetone ng 3: 2ml benzen Nh thm vo mi ng 1 git du hoc m, lc nh, quan st s ha tan ca lipid trong ba dung mi hu c. II. Kho st mt s ch s c trng ca cht bo 1. Ch s acid: L s mg KOH dng trung ha cc acid bo t do trong 1g cht bo. Nguyn tc Dng KOH 0,1N trong ru trung ha cc cht bo t do trong 1g nguyn liu, cht ch th mu l phenolphtalein. Dng c v ha cht Dng c Ha cht Erlen 100ml KOH 0,1N Pipet 10ml Ether ethylic Becher 100ml Phenolphtalein 0,5% trong alcohol Bnh tia Du kho st Cch lm

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Cn 1g (1ml) cht bo cho vo erlen, thm 10ml ether ethylic, lc u cho tan cht bo. Cho 1 git phenolphtalein v chun bng KOH 0.1N cho n khi xut hin mu hng bn trong 30 giy. Tnh ton kt qu: Ch s acid = 5.61 * V/m 5,61 l s mg KOH tng ng 1ml KOH 0.1N V l s ml KOH m l trng lng lipid cn (gram) 2. Ch s savon L s mg KOH cn thit savon ha cc ester cha trong 1g cht bo. Nguyn tc: Cho nguyn liu kt hp vi 1 lng tha KOH savon ha cht bo. nh lng KOH tha bng HCl gip suy ra ch s savon. Dng c v ha cht: Dng c Ha cht Erlen KOH 0.5N trong ru Pipet 10ml HCl 0.5N Becher 100ml Ethanol tuyt i B chng ct hon lu Phenolphtalein 0,5% trong alcohol Cht bo kho st Cch lm: Cn chnh xc 500mg cht bo cho vo erlen, thm 10ml KOH 0.5N. un si hon lu 45, nh phn KOH bng HCl 0.5N vi cht ch th mu l phenolphtalein. Thc hin mt bnh i chng thay 500mg cht bo bng 500mg nc. Tnh ton kt qu: Ch s savon = 28.05 * (V0 V1)/m 28.05 l s mg KOH tng ng 1ml HCl 0.5N V0 l th tch dung dch HCl 0.5N ca bnh i chng V1 l th tch dung dch HCl 0.5N ca bnh th nghim m l trng lng cht bo cn (gram)

NH LNG VITAMIN C
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(Phng php nh lng vitamin C bng IOD) 1. Nguyn tc xc nh nhanh chng hm lng vitamin C trong nguyn liu khi c cht mu ngi ta thng dng phng php chun iod. Tt c acid ascorbic b oxi ha bi iod. Phn iod tha s cho mu xanh vi dung dch tinh bt. iu ni ln l phn ng kt thc. 2. Dng c v ha cht Dng c
-

Ha cht

Erlen 100ml - Dung dch HCl 5% - Pipet 5ml, 10ml - Dung dch Na2S2O3 0,01N - Buret - Dung dch tinh bt 1% - Bnh nh mc 50ml - Dung dch iod 0,01N - Becher - Ci chy s - Phu thy tinh 3. Cch thc hnh a. Chun b mu vt: Cn N (g) mu vt v nghin nhanh chng trong ci s vi 5ml HCl 5%. Vitamin C rt d b oxi ha trong khng kh nht l khi c s hin din ca cc ion kim loi (Fe, Cu). V vy trong khi chun b mu phi ct hoc nghin bng dao khng r v lm nhanh. Dch chit thu c cho vo bnh nh mc 50ml v thm nc ct (ra ci chy v b vi ln bng nc ct ri vo bnh), khuy u ri lc. b. nh phn: - nh chun li dung dch iod N/100 vi dung dch Na2S2O3 0,01N tnh h s hiu chnh T ca dung dch iod. - Chun b hai erlen. Ht vo mi bnh 20ml dch chit c cha vitamin C, 5 -10 git dung dch h tinh bt 1% v chun ngay bng dung dch iod 0,01N n khi c mu xanh

4. Kt qu
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S mg vitamin C trong 1g mu vt c tnh nh sau:
x% = 0,00088 .a.T .V .100 v.N

a: th tch (ml) dung dch iod 0,01N dng khi chun . T: h s hiu chnh dung dch iod 0,01N vi dung dch Na2S2O3 0,01N V: th tch chung ca dch chit (50ml) v: th tch dch chit ly chun (20ml) N: trng lng mu phn tch 0,00088: s gram vitamin C tng ng vi 1ml dung dch iod 0,01N

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