Liquid Chromatograph nstruction Manual

Manual No. IM50031 December 1977, Rev. A

COPYRIGHT~ 1 977 BY WATERS ASSOCIATES, INC. PRINTED I N

THE UNITED STATES OF AMERICA. A L L RIGHTS RESERVED. THIS BOOK OR PARTS THEREOF MAY NOT BE REPRODUCED I N ANY FORM WITHOUT WRITTEN PERMISSION OF THE PUBLISHERS.

Address comments regarding this publication to: Waters Associates, Inc. Publications Department - MS Maple Street Milford, MA. 01747

Waters, The Liquid Chromatography People

SAFETY CONSIDERATIONS

Read This E n t i r e Manual First T o Assure Proper Operation O f T h e System

Always Observe Good, Safe Laboratory Practices When Operating T h e Preparative System

Wear Safety Glasses D u r i n g Preparative System Operation

Always Treat Solvents A s Hazardous, Keep T h e m Covered A n d Under A V e n t i l a t i o n H o o d A t A l l Times

Solvent Reservoirs Should Be Strongly Constructed F r o m A Chemically Resistant Material Such A s Glass O r Stainless Steel

Glass Reservoirs Should Be Placed I n Secondary Catch Containers I n Case O f Breakage

Use O n l y Non-sparking M o t o r s A n d Components Near Organic Solvents

Never Smoke Near Organic Solvents O r T h e Preparative System

Manual Purpose:

The scope of this manual deals primarily with the operational aspects of the PrepLC/System 500 Liquid Chromatograph. To this end extensive use of both verbal instructions and visual aids have been co-ordinated together on the following pages t o present a clear and comprehensive scheme for carrying out preparative separations on the PrepLC/System 500. This detailed scheme covers such operational aspects as starting the system, equilibrating the system column, loading the sample material onto the column, collecting the eluting sample fractions, and in the final phase of the separation process, shuting down the system for future start-ups. In a more limited way this manual also addresses itself t o a few other aspects of the PrepLC/System 500 that should augment the chromatographer's overall success in this new technology of high-speed, high purity-yield preparative chromatography. To this aim a concise guide is included t o help determine those problematical parameters necessary to achieve a given separation. Various techniques are indicated therein that may prove applicable in obtaining the best results in the most efficient manner for the given separation problem. In other sections information is provided for diagnosing and correcting those minor operational malfunctions that may result from accumulated contamination or from normal system wear after continued use. Moreover, a maintenance section is devoted to detailing the routine replacement and cleaning of those expendable items that have a limited duty life within the system. In the Appendix section further information is presented concerning system parts, internal layout, and schematics of the PrepLC/System 500.