Professional Documents
Culture Documents
Historical background
DNA fingerprinting was developed in 1984 by Alec. J. Jeffrey at the University of Leicester He was studying the gene of myoglobin.
The different sequences is the same as the word "POST" has a different meaning from "STOP" or "POTS," even though they use the same letters.
Using these sequences, every person could be identified solely by the sequence of their base pairs There are so many millions of base pairs, the task would be very time-consuming Instead, scientists are able to use a shorter method, because of repeating patterns in DNA. These patterns do not, however, give an individual "fingerprint," They are able to determine whether two DNA samples are from the same person, related people, or non-related people.
2: Cutting, sizing, and sorting. Special enzymes called restriction enzymes are used to cut the DNA at specific places
3: Transfer of DNA to nylon. The distribution of DNA pieces is transferred to a nylon sheet by placing the sheet on the gel and soaking them overnight. 4-5: Probing. Adding radioactive or colored probes to the nylon sheet produces a pattern called the DNA fingerprint.
4-6: DNA fingerprint. The final DNA fingerprint is built by using several probes (5-10 or more) simultaneously.
Practical Applications of DNA Fingerprinting 1.Paternity and Maternity Person inherits his or her VNTRs from his or her parents Parent-child VNTR pattern analysis has been used to solve standard fatheridentification cases
Crime
Forensic science is the use of scientific knowledge in legal situations. The DNA profile of each individual is highly specific. The chances of two people having exactly the same DNA profile is 30,000 million to 1 (except for identical twins).
Example
A violent murder occurred. The forensics team retrieved a blood sample from the crime scene. They prepared DNA profiles of the blood sample, the victim and a suspect as follows:
3. Personal Identification The notion of using DNA fingerprints as a sort of genetic bar code to identify individuals 4. Diagnosis of Inherited Disorders diagnose inherited disorders in both prenatal and newborn babies These disorders may include cystic fibrosis, hemophilia, Huntington's disease, familial Alzheimer's, sickle cell anemia, thalassemia, and many others.