PHN 2: XC NH HM LNG VITAMIN C

I,Nguyn tc.
Acid ascorbic ( Vitamin C ) l 1 hp cht cha no c cha nhm endiol. Acid ascorbic b ph hu rt nhan di tc dng ca cc cht oxy ho v bn trong mi trng acid . Phng php da trn nguyn tc l acid ascorbic c kh nng oxy ho kh thun nghch nh trong phn t ca n cha nhm endiol. V vy , acid ascorbic c xc nh bng phng php chun vi KIO3/KI theo phn ng sau: KIO3 + 5KI + 6HCl 3I2 + 6KCl + 3H2O Acid ascorbic + I2 Acid dehydroascorbic + 6HI

II. Ho cht Dng c.

o Ci chy s o Bnh nh mc 100ml o Pipette 10ml o Cc thu tinh 100ml o HCl 1% o KIO3/KI 0.001N o H tinh bt 1% o Phu thu tinh o Burette 25ml o Erlen 50ml

III . Cch tin hnh.

- Cn ly vo ci s lng sri chnh xc khong 3g vo ci gi v nghin nh cho n khi dung dch c dng ng th. Thm 1 lng HCl 1% va vo ci sri c hon ton ngm kn trong dung dch acid .Sau , chuyn ton b hn hp vo bnh nh mc 100ml. nh mc n vch bng dung dch HCl 1%. Lc cho lng acid ascorbic c trong nguyn liu c ho tan hon ton.

- Ly vo erlen 10ml dung dch cha Vitamin C t bnh nh mc , thm vi git tinh bt 1% v em nh phn bng KIO3/KI 0.001N ti khi xut hin mu xanh en . Lp li 3 ln c kt qu chnh xc. Sau ly kt qu trung bnh V (ml) dung dch KIO3/KI 0.001N dung chun vitamin C. - Tin hnh song song cc mu kim chng . Ht 10ml dung dch HCl 1% thm vi git h tinh bt 1% v em nh phn bng KIO3/KI 0.001N ti khi xut hin mu xanh en . Lp li 3 ln c kt qu chnh xc hn. Sau ly kt qu trung bnh V (ml) dung dch KIO3/KI 0.001N dung chun vitamin C.

IV.Kt lun.
Kt qu:

a: S ml KIO3/KI 0.001N dung nh phn dch chit vitamin C b: S ml KIO3/KI 0.001N dung nh phn mu kim chng. m: Lng mu th nghim ( Khi lng sri) X: hm lng vitamin C (mg/100g) 0.088: S mg acid ascorbic ng vi 1ml dung dch KIO3/KI 0.001N 100 : th tch bnh nh mc (ml)

m = 3g

a1= 0.7 ml a2 = 0.8 ml a3 =0.6 ml a = 0.7 ml

b1 = 0.4 ml b2 = 0.7 ml b3 = 0.8 ml b = 0.6 ml

Hm lng vitamin C trong mu th nghim c tnh bng cng thc: X = ((a b) x 0.088 x 100 x 100) / (10.m) = ( (0.7 0.6) x 0.088 x 100 x 100) / (10 x 3 ) = 2,93 ( mg/100g)