KIM TRA CHT LNG NG BNH KO

GVHD: NG TH CC

MC LC
Bi 1: KIM TRA NC MA .............................................................................. 3
1. o pH ca nc ma: ....................................................................................... 3
1.1.

Tin hnh: .................................................................................................. 3

1.2.

Kt qu:...................................................................................................... 3

1.3.

Nhn xt:.................................................................................................... 3

2. o Bx ca nc ma: ........................................................................................ 3
2.1.

Tin hnh: .................................................................................................. 3

2.2.

Kt qu:...................................................................................................... 3

3. o pol: .............................................................................................................. 3
3.1.

Tin hnh: .................................................................................................. 3

3.2.

Kt qu:...................................................................................................... 3

3.3.

Nhn xt:.................................................................................................... 3

4. Tnh AP: ...................................................................................................... 4

5. Hm lng RS: ................................................................................................. 4
5.1.

Tin hnh: .................................................................................................. 4

5.2.

Kt qu:...................................................................................................... 4

Bi 2: XC NH KIM CA BNH ........................................................... 5

1. Nguyn tc:....................................................................................................... 5
2. Tin hnh: ......................................................................................................... 5
Bi 3: KIM TRA CHT LNG CA KO .................................................... 6
1. Xc nh hm lng ng kh ca ko theo phng php Lane Eynon:... 6
1.1.

Nguyn tc: ................................................................................................ 6

1.2.

Tin hnh: .................................................................................................. 6

1.3.

Kt qu:...................................................................................................... 6

1.4.

Nhn xt:.................................................................................................... 7

2. Xc nh hm lng acid trong ko ................................................................. 7

2.1.

Nguyn tc: ................................................................................................ 7

2.2.

Tin hnh: .................................................................................................. 7

Mu ko cng: .................................................................................................. 7
Mu ko mm: .................................................................................................. 7
NHM 9

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KIM TRA CHT LNG NG BNH KO

2.3.

GVHD: NG TH CC

Kt qu: ..................................................................................................... 8

Bi 4: XC NH HM LNG NG TON PHN CA KO THEO

PHNG PHP BECTRAN ............................................................................... 9
1. Nguyn tc:....................................................................................................... 9
2. Tin hnh: ......................................................................................................... 9
2.1.

Chun b dung dch mu th: .................................................................... 9

2.2.

Thy phn dung dch th: ........................................................................ 10

2.3.

Xc nh hm lng ng: .................................................................... 10

2.4.

Kt qu:.................................................................................................... 10

Bi 5: KIM TRA NG NON MT ........................................................... 12

1. Pha long dung dch: ...................................................................................... 12
2. o Bx: ............................................................................................................ 12
3. o pol: ............................................................................................................ 12
4. Tnh AP: .................................................................................................... 12
5. Hm lng RS: ............................................................................................... 12
Bi 6: KIM TRA NG THNH PHM ..................................................... 14
1. m: ............................................................................................................ 14
1.1.

Tin hnh: ................................................................................................ 14

1.2.

Kt qu:.................................................................................................... 14

1.3.

Nhn xt:.................................................................................................. 14

1.4.

Bin lun: ................................................................................................. 14

2. o pol: ............................................................................................................ 14
2.1.

Tin hnh: ................................................................................................ 14

2.2.

Kt qu:.................................................................................................... 15

2.3.

Nhn xt:.................................................................................................. 15

2.4.

Bin lun: ................................................................................................. 15

3. Hm lng RS: ............................................................................................... 15

3.1.

Mu trng:................................................................................................ 15

3.2.

Mu l ng vng: ................................................................................. 15

3.3.

Mu l ng trng: ................................................................................ 16

NHM 9

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KIM TRA CHT LNG NG BNH KO

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Bi 1: KIM TRA NC MA
1. o pH ca nc ma:
1.1. Tin hnh:
Ly mu ngui n nhit phng em o pH bng my o pH.
pH = pH c c trn my
1.2. Kt qu:
pH nc ma = 5,2 ; Ti nhit : to = 31oC
1.3. Nhn xt:
Nc ma c pH = 5,2 < 7. Nc ma c mi trng axit yu, v th c v hi
chua.
2. o Bx ca nc ma:
2.1. Tin hnh:
o bng chit quang k, o nhit dung dch ti thi im c Bx.
Bx% = Bx c Bx
2.2. Kt qu:
Bx c = 21,5 ; Ti nhit : to = 31,5oC
Tra bng hiu chnh nhit ca Bx quan st (t 0 - 80 oC) nhit chun
20 oC : Ti 30 oC - Bx c = 20 - Bx = 0,68
S dng phng php ni suy c c:
Ti 31,5 oC - Bx c = 21,5 - Bx = 0,689
Bx% = Bx c Bx = 21,5 + 0,689 = 22,189
3. o pol:
3.1. Tin hnh:
Ly 150 ml nc ma cho vo bnh tam gic kh sch + 0,5g ch acetat bt.
Quan st nu c kt ta l c. Sau lc vo mt bnh tam gic kh sch khc.
Trng b 25ml dch lc u. Cho dch lc vo ng o pol 250mm v em o trong
my o pol. Kt qu c trn my ghi pol c.
pol % =
3.2. Kt qu:
pol c = 68
T Bx% tm c phn trn, ta bng i chiu nng Bxix, B v t
trng ca dung dch ng 20 oC:
Ti nng Bx% = 22,189 d = 1,09080
pol % =
3.3. Nhn xt:
Pol% < Bx%, bi v trong Bx cn c nhng cht ha tan khc ngoi pol.
NHM 9

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KIM TRA CHT LNG NG BNH KO

GVHD: NG TH CC

4. Tnh AP:
AP % =

x 100

Kt qu:
AP % =

x 100 = 73,2074

5. Hm lng RS:
5.1. Tin hnh:
Ht 5ml FA + 5ml FB + 20ml nc ct vo bnh tam gic 250ml, t ln bp
un si, nh vi git nethylen blue. nh phn bng dung dch nc ma mu cho
n khi dung dch chuyn mu xanh dng sang mu gch. Kim chng bng
methylene blue bng cch nh vo 1 git methylene blue nu mt nu xanh l phn
ng kt thc. Ghi ly V(ml) nc ma mu tiu tn.
RS % =
Trong :
f: factor ca dung dch fehling
V: s ml nc ma mu nh phn
d: t trng ca dugn dch suy t Bx
5.2. Kt qu:
f=1
d = 1,09080
Tin
Cm
V(ml)
2,6
2,6
RS % =

NHM 9

Lin
2,5

Yn
2,5

Vtb
2,55

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KIM TRA CHT LNG NG BNH KO

GVHD: NG TH CC

Bi 2: XC NH KIM CA BNH
1. Nguyn tc:
Trung ha lng kim c trong mu bnh bng dung dch HCl 0.1N vi ch th
Methyl da cam 0.1%.
2. Tin hnh:
Chun bi mu bnh quy
Cn 5g mu bnh quy nghin nh cho va bnh tam gic 250ml.
Thm vo 50ml nc ct, yn 30 pht, thnh thong lc u. Sau lc sang
bnh tam gic kh sch khc.
Ra giy lc nhiu ln v thu ton b nc ra xung bnh tam gic. Thm 3 git
M.O 1%.
Chun bng dung dch HCl 0.1N n mu cam th dng li v ghi th tch
dung dch HCl 0.1N tiu tn.
3. Kt qu chun :
Khi lng cn(g)
HCl 0.1N tiu tn(ml)
5.01
2.6
Yn
5.03
2.3
Lin
5.09
2.3
Tin
5.10
2.5
Cm
5.0575
2.425
Tng
Hm lng acid:
X (%)

NHM 9

N *V
0.1 * 2.425
*100
*100 0.4794%
m *10
5.0575 *10

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KIM TRA CHT LNG NG BNH KO

GVHD: NG TH CC

Bi 3: KIM TRA CHT LNG CA KO

1. Xc nh hm lng ng kh ca ko theo phng php Lane Eynon:
1.1. Nguyn tc:
ng kh c kh nng kh lm mt mu xanh metyl. V vy, c th dng
xanh metyl lm cht ch th cho phn ng oxy ha ng kh bng ehling. Cho vi
git metyl xanh vo dung dch ehling v un si ri nh tng git ng kh vo.
u tin ng kh s kh ng ca Fehling, mu ca metyl xanh khng i. Khi
tt c ng ca Fehling b kh ht th ng s kh metyl xanh lm cho n mt
mu. l du hiu kt thc qu trnh nh phn. Yu cu tin hnh nh phn
nhanh v lun gi trng thi si n nh.
1.2. Tin hnh:
1.2.1. Lm mu trng (lm chung):
Dng buret cho 8 9ml dung dch ng kh 1%, vo bnh tam gic 100ml,
thm 10ml Fehling A, 10ml Fehling B, 10ml nc ct. un si 1 pht, cho thm 3
5 git metyl xanh. Chun nng dung dch trong bnh tam gic bng dung dch
ng kh 1% cho n khi mt mu xanh th dng li. Thm vi git metyl xanh,
nu mu dung dch trong bnh tam gic khng i l phn ng kt thc. Ghi th
tch dung dch ng kh 1% tiu tn (V).
1.2.2. Lm mu chnh (mi ngi trong nhm thc hin th nghim 1 ln):
Cn (0,2g ko cng, 0,1g ko mm) nghin nh cho vo bnh tam gic
100ml. Thm 10ml Fehling A, 10ml Fehling B, 20ml nc ct. un si dung dch
trong bnh tam gic 1 pht, cho thm 3 5 git metyl xanh, lun gi bnh tam gic
nhit si. Chun nng dung dch trong bnh tam gic bng dung dch ng
kh 1% cho n khi mt mu xanh th dng li. Ghi th tch dung dch ng kh
1% tiu tn (V1).
1.3. Kt qu:
Hm lng ng kh ca ko tnh bng % theo cng thc sau:
%RS =

Trong :
V1: th tch dung dch ng kh 1% tiu tn trong mu chnh (ml)
V: th tch dung dch ng kh 1% tiu tn trong mu trng (ml)
m: khi lng ko (g)
Kt qu thc hnh:
m ko cng = 0,2 g
m ko mm = 0,1 g
V = (10,2 + 10)/ 2 = 10,1 ml

NHM 9

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GVHD: NG TH CC

Bng: Th tch dung dch ng kh 1% tiu tn trong mu chnh

SVTH
V1 ko cng (ml)
V1 ko mm (ml)
Cm
6,3
3,9
Tin
6,5
4,7
Lin
5,5
3,4
Yn
5,9
3,6
V1 trung bnh
6,05
3,9
Kt qu tnh ton:
Hm lng ng kh ca ko cng:
%RS =

Hm lng ng kh ca ko mm:
%RS =

1.4. Nhn xt:

Hm lng ng kh ca ko cng > Hm lng ng kh ca ko mm
2. Xc nh hm lng acid trong ko
2.1. Nguyn tc:
Dng dung dch NaOH 0.1N trung ha lng acid c trong mu ko vi ch
th phenolphtalein 1%.
2.2. Tin hnh:
Chun b 2 mu ko: mu ko cng (ko me), mu ko mm (ko sa).
Mu ko cng:
Mu ko cng nghin nh cn chnh xc 20g cho vo cc thy tinh, thm
100ml nc ct, dng a thy tinh khuy cho tan mu, rt vo bnh nh mc
250ml, dng nc ct trng ra cc chuyn ton b dung dch vo bnh nh
mc. Thm nc ct n vch, y np lc u.
em lc dung dch ny qua giy lc vo bnh tam gic kh sch. Trng b
phn dung dch lc u.
Ly chnh xc 25ml dung dch sau lc cho vo bnh tam gic 250ml, cho thm
100ml nc ct v 2-4 git ch th PP 1%
Chun bng dung dch NaOH 0.1N cho n mu hng nht, bn sau 30
giy th dng li. Ghi th tch dung dch NaOH tiu tn.
Mu ko mm:
Cn chnh xc 20g cho vo cc thy tinh, thm 100ml nc ct, dng a thy
tinh khuy cho tan mu, rt vo bnh nh mc 250ml, dng nc ct trng ra cc
NHM 9

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KIM TRA CHT LNG NG BNH KO

GVHD: NG TH CC

chuyn ton b dung dch vo bnh nh mc. Thm nc ct n vch, y np

lc u.
em lc dung dch ny qua bng vo bnh tam gic kh sch. Trng b phn
dung dch lc u.
Ly chnh xc 25ml dung dch sau lc cho vo bnh tam gic 250ml, cho thm
100ml nc ct v 2-4 git ch th PP 1%
Chun bng dung dch NaOH 0.1N cho n mu hng nht, bn sau 30
giy th dng li. Ghi th tch dung dch NaOH tiu tn.
2.3. Kt qu:
Th tch NaOH 0.1N tiu tn vi ko cng:
Ln 1
Ln 2
2.4ml
2.2ml
Yn
2.2ml
2.2ml
Lin
2.3ml
2.1ml
Tin
2.25ml
2.1ml
Cm
Tng
Hm lng acid:
X (%)

N * M *V 1*V 2
0.1 * 64 * 250 * 2.21875
*100
*100 0.71%
m * V *1000
20 * 25 *1000

Th tch NaOH 0.1N tiu tn vi ko mm:

Ln 1
Ln 2
0.4ml
0.4ml
Yn
0.4ml
0.45ml
Lin
0.35ml
0.4ml
Tin
0.4ml
0.35ml
Cm
Tng
Hm lng acid:
X (%)

Tng
2.3ml
2.2ml
2.2ml
2.175ml
2.21875ml

Tng
0.4ml
0.425ml
0.375ml
0.375ml
0.39375ml

N * M *V 1*V 2
0.1 * 64 * 250 * 0.39375
*100
*100 0.126%
m * V *1000
20 * 25 *1000

Ko cng (ko me) hm lng acid cao hn ko mm. Hm lng acid th

hin chua ca ko. Tng cm gic ngon v khng chn. Hm lng acid cho vo
trong ko ty vo loi ko khc nhau m ngi ta b sung lng acid khc nhau.

NHM 9

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GVHD: NG TH CC

Bi 4: XC NH HM LNG NG TON PHN CA KO THEO

PHNG PHP BECTRAN
1. Nguyn tc:
- Xc nh hm lng ng ton phn bng phng php Bectran. Lng
ng ton phn gm saccaroza v cc loi ng khc (lactoza, maltoza,
glucoza,) ca ko sau khi thy phn 700C trong 5 pht.
- Phng php Bectran da trn c s l trong mi trng kim cc ng
kh (glucoza, fructoza,) c th d dng kh ng II thnh ng I di dng kt
ta mu , qua tnh c lng ng kh.
- nh lng kh, thng dng thuc th Fehling l hn hp theo t l 1:1
ca dung dch ng sunfat- cn gi l Fehling A v dung dch kim ca mui
secnhet (mui tactrat kp kali-natri) cn gi l Fehling B. Khi trn dung dch F.A v
F.B th xy ra phn ng theo hai giai on.
+ Giai on 1: To thnh Cu(OH)2 + Na2SO4
+ Giai on 2: Cu(OH)2 tc dng vi mui secnhet to thnh mui phc ha
tan v dung dch c mu xanh thm. Mui phc ny l hn hp cht khng bn, v
th cc d9uong2 kh d dng kh oxit ng II thnh ng I to ra kt ta oxit ng
I mu v bn thn ng b oxi ha khi dung dch ng tc dng vi dung dch
Fehling.
- nh lng Cu2O, tin hnh qua hai bc:
+ Bc 1: Oxi ha Cu2O bng sunfat st III trong mi trng axit sunfuric.
Cu2O + Fe2(SO4)3 + H2SO4 = 2CuSO4 + 2FeSO4 + H2O
+ Bc 2: nh lng Fe2+ bng cch oxi ha n nh dung dch KmnO4 trong
mi trng axit.
10FeSO4 + 2KMnO4 + 8H2SO4 = 5Fe2(SO4)3 + 2MnSO4 + K2SO4 + 8H2O
T lng KMnO4 tiu tn trong nh phn, tnh c lng Cu2O v t
tnh hm lng ng trong dung dch bng cch tra bng t l gia ng v
KMnO4 0,1N.
2. Tin hnh:
2.1. Chun b dung dch mu th:
Cn mt lng mu th trn cn phn tch sao cho trong 100ml dung dch mu
th phi c khong 0,5g ng kh. Ha tan lng cn mu th bng 100ml nc
ct 60 - 700C, nu mu khng tan ht th un thm trong ni nc 60 700C. Dng
nc ct trng ra v chuyn ton b dung dch th vo bnh nh mc 250ml (ton
b lng nc trong bnh khong 150ml). dung dch ngui n nhit phng .
kt ta loi b nhng cht khng phi l ng, thm vo bnh 10ml dung dch
ZnSO4 1N nu lng cn mu di 5g v 15ml, nu lng cn mu trn 5g, lc u.
NHM 9

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GVHD: NG TH CC

Cho tip mt th tch dung dch NaOH 1N ng bng th tch dung dch NaOH 1N
dng trung ha th tch dung dch ZnSO4 1N nh trn vi ch th P.P 1%
trong mt th nghim ring bit. Lc u dung dch trong bnh v thm nc ct n
vch, lc u. yn 10 pht ri em lc qua giy lc kh, sch. Phn dung dch
u trng ra bnh v b i. Dung dch sau lc c gi l dung dch I.
2.2. Thy phn dung dch th:
Ly chnh xc 50ml dung dch (I) vo bnh nh mc 250ml, thm 50ml nc
ct, 7ml HCl m c (d = 1,19). Cm nhit k vo bnh v un trong ni cch
thy, gi nhit ca dung dch trong bnh l 700C trong 5 pht. Ly bnh ra lm
ngui nhanh di vi nc. Trung ha dung dch trong bnh nh mc bng dung
dch NaOH 20% vi ch th P.P 1% n khi dung dch c mu hng bn sau 30
giy. Thm nc ct n vch, lc u. Dung dch ny gi l dung dch (II).
2.3. Xc nh hm lng ng:
Ly chnh xc 25ml Fehling A, 25ml Fehling B vo bnh tam gic 250ml.
Thm 25ml dung dch (II), lc u. t bnh tam gic ln bp in v un si 3 pht
k t lc bt u si. Ly ra lng kt ta. Dung dch bn trn ca ng oxit1 phi
c mu xanh, nu khng phi lm li vi dung dch (II) t hn. Khi kt ta ng oxit
lng xung, lc gn phn nc trn kt ta vo phu lc. Cho nc ct un si
vo bnh tam gic v tip tc lc gn cho n khi nc trong bnh ht kim tnh.
Trong khi lc lun gi mt lp nc trn kt ta trnh ng oxit tip xc vi
khng kh. Sau khi lc xong cho phn giy lc va lc xong vo bnh tam gic c
kt ta ng oxit, tip tc cho thm 40ml dung dch st III sunfat. Chun ngay
dung dch trong bnh tam gic bng dung dch KMnO4 0,1N cho ti khi dung dch
trong bnh chuyn sang mu pht hng th dng li. Ghi li th tch dung dch
KMnO4 0,1N tiu tn.
2.4. Kt qu:
Sau khi chun ta c:
= 11,5 ml
Tra bng lng glucoza theo KMnO4 ta c m1= 37.
Hm lng ng ton phn tnh bng % theo cng thc:
X4 =

(%)

Trong :
- m: khi lng mu, tnh bng g.
- m1: khi lng ng tra trong bng ph lc 10, tnh bng mg.
- V1: th tch dung dch ha tan lng mu cn (dd I), tnh bng ml.
- V2: th tch pha long dung dch sau khi nghch chuyn (dd II), tnh bng ml.
- V3: th tch dung dch (I) ly thy phn, tnh bng ml.
NHM 9

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KIM TRA CHT LNG NG BNH KO

GVHD: NG TH CC

- V4: th tch dung dch (II) ly xc nh hm lng ng, tnh bng ml.
Ta c:
V1 = 250ml
V2 = 250ml
V3 = 50ml
V4 = 25ml
m = 10g
m1 = 37mg
Hm lng ng ton phn:
X4 =

NHM 9

= 18,5 %

Page 11

KIM TRA CHT LNG NG BNH KO

GVHD: NG TH CC

BI 5: KIM TRA NG NON MT

Mc ch: Kim tra cht lng cc thng s k thut iu chnh dy truyn
sn xut cho ph hp, trnh t tht ng.
1. Pha long dung dch:
ng non: 50g ng + 150ml nc ct HSPL = 4
2. o Bx:
- Cch tin hnh: ly dung dch trn pha long o bng chit quang k
c kt qu o c.
- Kt qu o c: Bx c = 230
- Kt qu: Bx% = Bx c x HSPL = 23 x 4 = 92
3. o pol:
- Cch tin hnh: Cn 26g dung dch pha long vo bnh 100ml + 3ml
axetat ch nh mc n vch 100ml lc u lc dung dch vo bnh
tam gic kh (trng b 25ml dch lc u) ly dch lc em o pol trong
ng 200mm ghi li kt qu.
- Kt qu o c: Pol c = 17,10
- Kt qu: Pol% = pol c x HSPL = 68,4
4. Tnh AP:

5. Hm lng RS:
Cch 1:
- Cch tin hnh: ly dung dch pha long cho vo burette cho 5ml
Fehling A + 5ml Fehling B + 20ml nc ct vo bnh tam gic 250ml un
si mnh khi bt u si cho 1 2 git methylene blue chun bng
dung dch mu cho n khi dung dch chuy sang mu gch kim
chng bng methylene blue ghi li th tch chun .
- Cng thc tnh:

Trong :
V: th tch dung dch mu dung trong nh phn (ml)
D: t trng ca dung dch pha long suy t Bx
F: Factor ca dung dch Fehling
HSPL: H s pha long mu
Kt qu nhn c:
Ln chun
1
2
2,9ml
2,9ml
S ml chun
Cm
Yn
Ngi chun
Vtb = 2,875ml
NHM 9

3
2,8ml
Lin

4
2,9ml
Tin

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KIM TRA CHT LNG NG BNH KO

GVHD: NG TH CC

Bx = 230 d = 1,09636
F= 1
HSPL = 4

Cch 2:
- Cch tin hnh: Cn chnh xc 26,00g t dung dch pha long (ly dung dch
ca cch 1 pha long) cho vo bnh nh mc 100ml nh mc n vch
lc u chun bng dung dch ny dung dch t mu xanh chuyn sang
mu gch kim chng bng methylene blue ghi li th tch chun .
- Cng thc tnh:

Trong :
V: th tch dung dch mu dung trong nh phn (ml)
HSPL: H s pha long mu
Kt qu nhn c:
Ln chun
1
2
3
12,4ml
12,5ml
12,3ml
S ml chun
Yn
Tin
Lin
Ngi chun

Vtb = 12,425ml
HSPL = 4

4
12,5ml
Cm

Nhn xt: Xc nh RS bng 2 phng php cho ta kt qu sp s nhau, v

mt l thuyt 2 s ny phi ging nhau nhng kt qu ny vn c th chp nhn
c. Sai s ca 2 phng php ny do qu trinh th nghim cn cha chnh xc,
thiu cn thn khi thc hin cc thao tc th nghim.

NHM 9

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Bi 6: KIM TRA NG THNH PHM

Mc ch: Nhm kim tra cht lng ng thnh phm t yu cu, trnh
trng hp tung ra th trng sn phm khng t tiu chun cht lng (gy mt
uy tn ca cng ty). Cung cp s liu cho sn xut iu chnh cc thng s k
thut cho ph hp vi sn xut sn xut ra sn phm t cht lng quy nh v
m bo hiu qu thu hi ca nh my.
1. m:
1.1. Tin hnh:
Cn chnh xc 5,000g mu ng ct vng cho vo chn sy (m1) sy
chn nhit 1050C trong 2 gi lm ngui trong bnh ht m cn li trong
lng (m2) lp li qu trnh trn cho n khi thu c khi lng khng i (mi
ln sy tip theo l 30 pht).
Cng thc tnh:

1.2. Kt qu:
m1 = 30,7708g
m2 = 30,6975g
mmu = 5,0076g

1.3. Nhn xt:

m ca mu tnh c cao hn sao vi mu ng vng tinh luyn c
m 1,0 % khi lng. Cao hn: 1,4638 1,0 = 0,4638%.
1.4. Bin lun:
- Mu ng b ht nc m qu cao.
- Bo qun khng tt ni kh ro.
- ng ngoi khng kh d b ht m.
- Sy cha trit lng m c trong mu ng.
2. o pol:
2.1. Tin hnh:
Cn 26g mu ha tan bng nc ct cho vo bnh nh mc 100ml
trng ra cc nhiu ln, tt c nc ra cho vo bnh nh mc dng nc ct
nh mc ln 100ml lc u lc vo bnh tam gic kh sch trng b 25ml
dch lc u dch lc em o pol trong ng 200mm v o nhit lc o pol.
Cng thc tnh:
Pol% = pol c x [1 + 0,0003 x (t 20 )]

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2.2. Kt qu:
Pol c = 96 - Nhit = 310C
Pol% = 96 x [1 + 0,0003 x (31 20)] = 96,3168
2.3. Nhn xt: Vi pol = 96 l thp kt qu cha ng.
2.4. Bin lun: Trong qu trnh th nghim c th xy ra cc sai st.
- Cn mu cha chnh xc.
- mu ngoi khng kh qu lu trc khi o mu.
- Lc mu cha k.
- Dng cng cha mu vn cn nc khi rt mu vo dn n mu b pha
long nhng khng th xc nh c h s.
3. Hm lng RS:
3.1. Mu trng:
Cch tin hnh:
10ml FA + 10ml FB + 20ml nc ct un si trn bp in nh 3 git
Methylen blue nh phn bng dung dch glucose 5% cho n khi dung dch
t mu xanh chuyn sang mu gch kim chng bng Methylen Blue ghi
ly V(ml) = A
Kt qu thu c:
V1 = 23,1ml
V2 = 24,9ml
Vtb = 24 = A
3.2. Mu l ng vng:
Cch tin hnh:
Bc 1: Cn chnh xc 26g mu ng vng ha tan bng nc ct cho vo
cc thy tinh 100ml cho vo bnh nh mc 100ml trng ra cc nhiu ln,
dung nc ct nh mc ln vch lc u.
Bc 2: 10ml FA + 10ml FB + 20ml nc ct + 10ml dung dch mu un si
trn bp in nh 3 git Methylen blue nh phn bng dung dch glucose 5%
cho n khi dung dch t mu xanh chuyn sang mu gch kim chng
bng Methylen Blue ghi ly V(ml) = a
Cng thc tnh
(
)
F: factor ca dung dch fehling
Kt qu thu c:
Ln chun
1
S ml chun 3,4ml
Yn
Ngi chun

Vtb = 3,425 ml = a
F=1

2
3,5ml
Tin

3
3,3ml
Lin

(
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4
3,5ml
Cm

)
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Nhn xt:
Hm lng RS tnh c sau khi tin hnh th nghim cao hn so vi ng
vng tinh khit vi hm lng ng kh < 0,13%
Bin lun:
- C th do hm lng kh trong mu qu cao, sn phm khng t cht lng
yu cu.
- Chun s ml th tch vt qu lng ng kh trong mu, dn n sai
s.
3.3. Mu l ng trng:
Cch tin hnh:
Bc 1: Cn chnh xc 26g mu ng trng ha tan bng nc ct cho vo
cc thy tinh 100ml cho vo bnh nh mc 100ml trng ra cc nhiu ln,
dung nc ct nh mc ln vch lc u.
Bc 2: 10ml FA + 10ml FB + 20ml nc ct + 20ml dung dch mu un si
trn bp in nh 3 git Methylen blue nh phn bng dung dch glucose 5%
cho n khi dung dch t mu xanh chuyn sang mu gch kim chng
bng Methylen Blue ghi ly V(ml) = a
Cng thc tnh
(
)
F: factor ca dung dch fehling
Kt qu thu c:
Ln chun
1
S ml chun 22,6ml
Yn
Ngi chun

Vtb = 22,475 ml = a
F=1

2
22,5ml
Tin

3
22,4ml
Lin

4
22,4ml
Cm

Nhn xt:
Hm lng RS tnh c sau khi tin hnh th nghim cao hn khng nhiu so
vi ng tinh luyn vi hm lng ng kh < 0,03%, vi kt qu ny sp s
bng nhau.

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