TIU CHUN VIT NAM

TCVN 7871-1:2008
HT LA M V BT M - HM LNG GLUTEN - PHN 1: XC NH GLUTEN T BNG
PHONG PHP TH CNG
Wheat and wheat flour - Gluten content - Part 1: Determination of wet gluten by a manual method
Li ni u
TCVN 7871-1:2008 hon ton tuong duong vi SO 21415-1:2006;
TCVN 7871-1:2008 do Ban k thut tiu chun TCVN/TC/F1 Ng cc v !u " bin soan, Tng
cuc Tiu chun o lung Cht luong d nghj, B Khoa hoc v Cng ngh cng b.
B TCVN 7871:2008 (SO 21415:2006) #$t l%a m& v b't m& - #m l()ng gluten, bao gm cc
phn sau dy:
- TCVN 7871-1:2008 (SO 21415-1:2006) #$t l%a m& v b't m& - #m l()ng gluten - Ph*n 1: +,c
-nh gluten (.t b/ng 0h(1ng 0h,0 th2 c3ng4
- TCVN 7871-2:2008 (SO 21415-2:2006) #$t l%a m& v b't m& - #m l()ng gluten - Ph*n 5: +,c
-nh gluten (.t b/ng 0h(1ng 0h,0 c1 h6c4
- TCVN 7871-3:2008 (SO 21415-3:2006) #$t l%a m& v b't m& - #m l()ng gluten - Ph*n 7: +,c
-nh gluten 8h3 t9 gluten (.t b/ng 0h(1ng 0h,0 :;y4
- TCVN 7871-4:2008 (SO 21415-4:2006) #$t l%a m& v b't m& - #m l()ng gluten - Ph*n <: +,c
-nh gluten 8h3 t9 gluten (.t b/ng 0h(1ng 0h,0 :;y nhanh=
Li gii ti!u
Cc k thut thay th quy djnh trong tiu chun ny hoc TCVN 7871-2:2008 (SO 21415-
2:2006) d tch gluten ut (ngha l phuong php ra th cng v ra co hoc) thung khng
cho kt qu ging nhau. khi phuc hon ton cu trc gluten cn phi d yn khi bt nho.
Khi la m cha hm luong gluten cao, kt qu thu duoc bng phuong php ra th cng
thung cao hon kt qu thu duoc bng phuong php ra co hoc. Do d, trong bo co th
nghim cn phi nu r k thut ra d dng.
H"T L#A M$ V% &'T M$ - H%M L()N* *LUT+N - ,H-N 1: ./C 01NH *LUT+N (2T &3N*
,H(4N* ,H/, TH5 C6N*
Wheat and wheat flour - Gluten content - Part 1: Determination of wet gluten by a manual
method
17 ,89 :i ;< =>ng
Tiu chun ny quy djnh phuong php ra th cng d xc djnh hm luong gluten ut ca bt
m (>riticum ae:tivum L. v >riticum durum Desf.). Phuong php ny c th p dung truc tip
cho bt m. Phuong php ny cng c th p dung cho li hat la m dang tm v hat la m sau
khi xay, nu su phn b c hat dp ng duoc cc yu cu nu trong Bng B.1.
27 T?i @i!u :i!n =An
Cc ti liu vin dn sau l rt cn thit cho vic p dung tiu chun ny. i vi cc ti liu vin
dn ghi nm cng b th p dung phin bn duoc nu. i vi cc ti liu vin dn khng ghi
nm cng b th p dung phin bn mi nht, bao gm c cc sa di.
SO 712, Cereals and cereal products - Determination of moisture content - Routine reference
method (Ng cc v sn phm ng cc - Xc djnh d m - Phuong php chun).
B7 TuCt ngD :? En ngFG
Trong tiu chun ny p dung cc thut ng v djnh ngha sau dy:
B717 *@utHn It (wet gluten)
Khi do dn hi, duoc tao thnh ch yu t hai phn protein (gliadin v glutenin) dang hon
nguyn thu duoc bng phuong php quy djnh trong tiu chun ny hoc TCVN 7871-2:2008
(SO 21415-2:2006).
B727 H8t @JG 9K ngiLn (ground wheat)
Sn phm thu duoc bng cch nghin la m nguyn hat, dp ng duoc cc yu cu v c hat
nhu trong Bng B.1.
B7B7 LMi 8t @JG 9K dang tm (semolina)
Ni nh hat la m duoc nghin th.
B7N7 &Ot 9K (flour)
Ni nh hat la m duoc nghin mjn c c hat nh hon 250 m.
N7 NguPQn tRS
Chun bj bt nho t mu bt m hoc li hat la m dang tm hoc hat la m d nghin vi
dung djch natri clorua. Bt nho duoc gi yn d hnh thnh cu trc gluten. Khi bt nho ra
th cng vi dung djch natri clorua, sau d dung djch ra duoc loai b d tch gluten ut. Cn
phn cn thu duoc.
T7 TuUS tV
Thuc th duoc s dung phi l loai tinh khit phn tch, nuc duoc s dung phi l nuc ct
hoc nuc c d tinh khit tuong duong, tr khi c quy djnh khc.
T717 Wung =ES nGtXi S@YXuGZ 20 g/l
Ha tan 200 g dung djch natri clorua (NaCl) trong nuc, sau d pha long dn 10 l.
T727 Wung =ES iUt tXYng [G@i iY=uG \=ung =ES LugY@]
Ha tan 2,54 g kali iodua (Kl) trong nuc. Thm 1,27 g it (l2) vo dung djch ny, sau khi ha tan
hon ton, pha long bng nuc dn 100 ml.
^7 Ti_t `EZ =>ng S>
S dung cc thit bj, dung cu ca phng th nghim thng thung v cu th nhu sau:
^717 CUi `ang bcZ duoc trng men bn trong hoc `;t [i9 @Y8i tX;ng 9Hn c dung knh t 10
cm dn 15 cm.
^727 &uXHtZ dung tch 25ml, chia vach 0,1 ml.
^7B7 &Kn S 9dZ dung tch 250 ml.
^7N7 WGY tXOnZ bng cht do hoc thp khng g, di t 18 cm dn 20 cm.
^7T7 0FG teP tinZ kch thuc khong 40 cm x 40 cm.
^7^7 *fng tGPZ bng cao su mng v c b mt tron.
^777 ghP S [ung giZ kch thuc khung khong 30 cm x 40 cm, duoc ph lui vi kch thuc
l No.56 (kch thuc mt lui 308 m).
^787 W>ng S> ScGZ c van diu chnh dung djch natri clorua (5.1).
^7j7 *ikP @lS
1]
, djnh luong 120 g/m
2
.
1)
V du nhu giy loc Macherey Nagel s 651 l ph hop cho muc dch ny. Thng tin ny dua ra
tao thun loi cho ngui s dung Tiu chun cn SO khng n djnh phi s dung sn phm d.
^7107 0mng m `k9 gi7
^7117 ChnZ c th cn chnh xc dn 0,01 g.
^7127 W>ng S> n< g@utHnZ xem Phu luc A.
^71B7 opn mng mZ dung knh 8 cm.
^71N7 M;P ngiLn Sq nd, c kh nng nghin dn c hat theo yu cu trong Bng B.1.
77 LkP 9Au
Mu duoc gi ti phng th nghim phi l mu dai din. Mu khng duoc hu hng hoc thay
di trong qu trnh vn chuyn hoc bo qun.
Vic ly mu khng quy djnh trong tiu chun ny. Nn ly mu theo SO 6644 hoc TCVN 5451
(SO 1360).
87 Curn `E 9Au tV
ng ha mu v xc djnh d m theo SO 712. Truc khi xc djnh hm luong gluten, dng my
nghin c nh (6.14) d nghin hat la m v li hat dang tm theo quy djnh trong Phu luc B. Cn
dc bit ch trong sut qu trnh nghin v bo qun d khng lm thay di d m ca mu.
j7 C;S ti_n ?n
j717 sQu Su Sung
S dung dung djch natri clorua (5.1) d chun bj v ra bt nho.
Mu th v dung djch natri clorua phi duoc gi t nht qua mt dm trong phng th nghim
noi tin hnh th. Nu nhit d phng thp hon 20
o
C hoc cao hon 25
o
C, th nhit d ca mu
th v ca dung djch natri clorua phi duoc diu chnh dn khong t 20
o
C dn 25
o
C.
j727 ,n 9Au tV
Cn khong 24 g mu th chnh xc dn 0,01 g (m1? v chuyn luong mu ny sang ci nghin
hoc bt kim loai trng men (6.1).
j7B7 Curn `E `Ot n?Y :? t PQn
j7B717 Dng buret (6.2) ly 12 ml dung djch natri clorua (5.1) cho tng giot mt vo khi bt nho,
trong khi vn khuy lin tuc bng dao trn (6.4).
j7B727 Sau khi thm dung djch natri clorua, dng dao trn p hn hop v tao thnh khi bt nho
hnh cu, cn thn d trnh tht thot bt nho. Phn bt nho cn lai dnh trn thnh bt hoc
trn dao trn phi duoc ly ht cho vo khi bt.
j7B7B7 Thi gian chun bj bt nho khng duoc qu 3 min.
j7B7N7 t khi bt hnh cu vo da thy tinh (6.5). Dng giy loc ut (6.) ph vo phn trong
cc c m 250 ml (6.3), sau d dng d boc lun khi bt nho hnh cu. yn khi bt trong
30 min.
j7N7 gVG
j7N717 Cc thao tc m t trong .4.2 v .4.3 phi duoc tin hnh trn khung g c ph lui
thp mjn (6.7) d trnh tht thot bt nho. Trong sut qu trnh thao tc, ngui thuc hin phi
deo gng tay cao su (6.6) d bt nho khng hp thu nhit v thm m hi t lng bn tay.
j7N727 Sau khi d yn, cn khong 30 g bt nho (.3) chnh xc dn 0,01 g @m5?. t phn bt
nho ny vo lng mt bn tay v d cho dung djch natri clorua (5.1) dung trong vt cha (6.8)
chy nh giot vo khi bt nho vi tc d chy 750 ml trong 8 min. Trong sut thi gian ny,
dng ngn tay ci ca bn tay khc v trn khi bt nho.
j7N7B7 u trnh ra duoc coi l hon tt khi dung djch natri clorua duoc p ra t vin gluten thu
duoc trong .4.2 khng c tinh bt. pht hin tinh bt, nh vi giot dung djch ra p ra t vin
gluten ln knh dng h (6.13) v thm mt vi giot dung djch kali iodua/dung djch it (5.2). Nu
mu sc ca dung djch khng thay di th qu trnh ra d hon thnh. Nu mu ca dung djch
chuyn sang mu xanh th chng t vn cn tinh bt v phi tip tuc qu trnh ra cho dn khi
khng cn pht hin thy tinh bt.
j7N7N7 Thi gian ra phu thuc vo luong gluten, nhn chung khong 8 min l d.
j7T7 LY8i `d =ung =ES XVG Sun =I
j7T717 Loai b hu ht dung djch ra bm dnh vo vin gluten bng cch gi vin gluten gia
cc ngn tay v bp manh khong ba ln.
j7T727 Chia vin gluten thnh hai phn xp x bng nhau, djnh hnh chng trong mt khun dt
mng v dt chng vo dung cu p gluten (6.12). ng dung cu p gluten v m ra sau 5 s.
Chuyn gluten d dt mng, khng bj bin dang, vo vj tr kh khc trong dung cu p gluten v
dng lai trong 5 s. Lp lai thao tc ny 15 ln. Lm kh da thy tinh ca dung cu p sau mi ln
thuc hin.
j7^7 .;S En [Ui @Ivng g@utHn It
Cn c hai phn gluten d dt mng tao ra trong .5.2, chnh xc dn 0,01 g (m7).
j777 wU @n x;S En
Tin hnh hai ln xc djnh trn cng mt mu th.
107 Tpn tY;n :? `itu tE [_t yuz
Hm luong gluten ut, (Gwet), biu thj bng phn trm khi luong phn mu th, tnh bng cng
thc sau dy:
% 100
) 12 (
1 2
1 3

+
=
m m
m m
G
wet
trong d
m1 l phn khi luong th ca mu th (.2), tnh bng gam;
m5 l phn khi luong ca vin bt nho (.3) d dng d ra (.4), tnh bng gam;
m7 l khi luong ca gluten ut (.6), tnh bng gam;
12 l th tch ca dung djch natri clorua (5.1) dng d chun bj bt nho, tnh bng mililt;
Ly kt qu dn mt ch s sau du phy.
CH THCH 1: Sai lch v trong luong ring ca dung djch natri clorua (5.1) t 1,00 l khng
dng k so vi d lch chun ca phuong php.
CH THCH 2: Nhn chung kt qu ca php xc djnh khng tnh theo hm luong cht kh ca
mu th (bt m hoc li hat la m dang tm hoc hat la m). Do d, trong bo co th nghim
nn ghi lai d m ca mu th.
117 0O S>9
11717 ,n< tV @iQn <ung tV ngi!9
Chi tit ca php th lin phng th nghim v d chum ca phuong php ny duoc nu trong
Phu luc C. Cc gi trj thu duoc t php th lin phng th nghim ny c th khng p dung
duoc cho cc di nng d v cht nn khc vi cc di nng d v cht nn d nu.
11727 0O @{< @8i
Chnh lch tuyt di gia hai kt qu th dc lp, don l thu duoc khi s dung cng mt
phuong php trn vt liu th ging ht nhau trong cng mt phng th nghim, do cng mt
ngui tin hnh, s dung cng mt thit bj trong thi gian ngn, khng qu 5 cc trung hop
ln hon cc gi trj sau dy:
- di vi hat la m: r 1, g/100g;
- di vi bt m r A 1,5 g/100g;
- di vi hat la m cng r 0, g/100g;
- di vi li hat la m cng dang tm r 2,1 g/100g
117B7 0O t;i @C<
Chnh lch tuyt di gia hai kt qu th don l thu duoc khi s dung cng mt phuong php
trn vt liu th ging ht nhau trong cc phng th nghim khc nhau, do nhng ngui khc
nhau thuc hin, s dung cc thit bj khc nhau, khng qu 5 cc trung hop ln hon cc gi
trj sau dy:
- di vi hat la m: B ,5 g/100g;
- di vi bt m B A 7,7 g/100g;
- di vi hat la m cng B 14,0 g/100g;
- di vi li hat la m cng dang tm B 11,7 g/100g
127 &;Y S;Y tV ngi!9
Bo co th nghim phi ghi r:
a) tt c cc thng tin cn thit d nhn bit mu;
b) phuong php ly mu duoc s dung, nu bit;
c) phuong php th d s dung, vin dn tiu chun ny, bao gm nhng chi tit ca qu trnh
nghin;
d) moi thao tc khng quy djnh trong tiu chun ny, hoc duoc coi l ty chon, cng vi cc chi
tit bt k m c th nh hung dn kt qu;
e) cc kt qu th thu duoc;
f) nu dp ng duoc cc yu cu v d lp lai th nu kt qu cui cng thu duoc.
,H| L|C A
(quy djnh)
DNG C P GLUTEN
Kch thuc tnh bng milimet
,H| L|C &
(quy djnh)
CHUN B HT LA M NGHN
Nhu d d cp trong phn pham vi p dung ca tiu chun ny th phuong php cng c th p
dung duoc cho hat la m v li hat la m dang tm sau khi nghin trong my nghin c nh
(6.14).
Kch c hat mu nghin c nh hung dn su hnh thnh gluen v qu trnh ra. B nghin
duoc s dung phi c kh nng nghin sao cho dp ng duoc cc yu cu nu trong Bng B.1.
&zng &71 - Li XhP :? Sq 8t SeG 9Au
Li XhP
9
MAu @lt yuG XhP
}
710 100
500 5 dn 100
210 dn 200 80 hoc nh hon
S liu nu trong Bng B.1 duoc s dung d diu chnh dung cu nghin. Vic nghin phu thuc
vo loai php xc djnh. mjn ca bt phi duoc kim tra thung xuyn bng ry.
Phuong php nghin d chun bj mu nghin t hat la m hoc li hat dang tm c nh hung
dn kt qu xc djnh gluten. Cc loai dung cu nghin khc nhau, tao ra cc phn mu nghin
khc nhau vi cc thnh phn khc nhau, diu ny dn dn su hnh thnh gluten trong khi bt
nho v ra gluten cng khc nhau. thu duoc cc kt qu tuong duong cn s dung cng
mt phuong php chun bj mu. Ghi lai chi tit ca qu trnh nghin cng vi phn trm gluten
thu duoc.
Mu dai din ca hat la m hoc li hat la m dang tm phi duoc nghin dn kch c hat dp
ng duoc cc yu cu nu trong Bng B.1. Cho hat la m hoc li hat la m dang tm vo my
nghin phi cn thn d trnh qu nhit v qu ti. Thi gian nghin phi duoc tip tuc trong
khong t 30 s dn 40 s sau khi mu cui cng d duoc dua vo my nghin. Cc luong nh
(dn 1) hat la m hoc li hat la m dang tm cn duoc ly ra d nghin.
,H| L|C C
(tham kho)
KT U CA PHP TH LN PHNG TH NGHM
Mt php th lin phng th nghim bao gm 21 phng th nghim ca 7 quc gia, do Cng
ty CONCORDA arehouse, Phng th nghim kim tra hat ng cc, Budapest (Hungari) (Grain
Control Laboratory) t chc nm 2004. Php th ny duoc thuc hin trn su mu sau dy:
- Mu A: hat la m (>riticum ae:tivum L.);
- Mu B: hat la m (>riticum ae:tivum L.);
- Mu C: hat la m (>riticum durum Desf.);
- Mu D: li hat la m cng dang tm;
- Mu E: bt m;
- Mu F: bt m.
Cc kt qu thu duoc d duoc phn tch thng k theo TCVN 610-1 (SO 5725-1) v TCVN
610-2 (SO 5725-2) d cho cc s liu v d chum nu trong Bng C.1.
TI 9>S t?i @i!u tG9 [zY
1 SO 6644, Flowing cereals and milled cereal products - Automatic sampling by mechanical
means.
2 TCVN 5451:2008 (SO 1360:1), Ng cc, du d v sn phm nghin ly mu t khi
hng tnh.
3 TCVN 7871-2:2008 (SO 21415-2:2006), Hat la m v bt m - Hm luong gluten - Phn 2:
Xc djnh gluten ut bng phuong php co hoc.
4 CC Standard No. 106/2, orking Method for the Determination of et Gluten in heat Flour.
CJ gizi
1. da thy tinh c b mt th rp
2. gi d
3. vnh kim loai
CH THCH: uoc ly t Thu muc ti liu tham kho 4.