Charleen Joyce C.

Usacdin Analysis of Lipids

A. Spot Test 2.
Drop of test material on data sheet

August 30, 2013

Test for Free Glycerol 2 ml 5% CuSO4 2ml 10% NaOH 5-6 drops sample

Allow to dry; note transparency

(+) transparent Presence of volatile oils

(-) opacity

(+) dissolution of blue precipitate

B. Iodine Absorption 1 drop sample in 1ml Chloroform *Hubls solution - drop by drop - Shake test tube after each addition until solution no longer decolorize - Record the number of drops

(-) light blue gelatinous precipitate

D.

Test for Soap *Soap solution - dissolve 0.5ml of liquid soap in water 1. Surface tension 1ml distilled water - compare no of drops that will be formed from 1 ml pipette - Without rinsing fill again the pipette with distilled water - Note number of drops

sink

Soap solution

(+) disappearance of purple color

(-) purple color persists

Metal waste

(+) distilled waterless no of drops

(+) pipet with soap nore no of drops

C.

Test for Glycerol 1. Acrolein test

Pinch of Sodium bisulfite - in dry test tube sink

2. 2 drops test material

Salting out of soaps 20 ml soap solution

10 ml saturated NaCl

- Heat slowly at first; then vigorously - Note characteristic odor - Strip of paper moistened with ammoniacal silver nitrate solution over the mouth of test tube - Tightly stopper; continue heating

(+) acrid irritating odor; darkening of filter paper

(-) no odor; no darkening of filter paper

collect precipitate press out mother liquior Dissolve in 10 ml hot H2O Take 3ml 10 % HCl

solid waste

(+) precipitation upon addition of NaCl and upon acidification

(-) clear colorless solution

sink

Charleen Joyce C. Usacdin Analysis of Lipids

3. Formation od insoluble soap 5 ml soap solution Drops of CaCl2 TS - Repeat procedure with MgSO4 TS 2. Salkowski Test

August 30, 2013

Few crystals of cholesterol in 0.5 ml Chloroform

Equal volume of H2SO4

(+) white insoluble curds

(-) clear colorless solution

sink

(+) bluish red to cherry purple in the chloroform layer; phosphorescence in acid layer

(-) clear colorless solution

4.

Soap stabilize emulsion of fat in water

Test tube 1 Test tube 2 Test tube 3 Test tube 4

Acid waste

10 ml water

10 ml water

3.

Formaldehyde-sulfuric acid Test

Cooking oil

Cooking oil

Few crystals of cholesterol in 0.5 ml Chloroform Soap soution

10% Na2CO3

Shake to emulsify fatStand for 30min-

Shake to emulsify fatStand for 30min1 ml formaldehyde

Two layers of liquid

emulsion

sink (+) cherry red color in chloroform layer

(-) clear colorless solution

E.

Test for cholesterol 1. Liebermann-Burchard Test

Few crystals of cholesterol in 0.5 ml Chloroform

Acid waste

- Dry test tube 5 ml acetic anhydride

1-2 drops H2SO4

(+) red then blue and finally bluish green solutiom

(-) clear colorless solution

Acid waste

Charleen Joyce C. Usacdin Analysis of Lipids

August 30, 2013

Charleen Joyce C. Usacdin Analysis of Lipids

August 30, 2013

Charleen Joyce C. Usacdin Analysis of Lipids

August 30, 2013

Charleen Joyce C. Usacdin Analysis of Lipids

August 30, 2013