Professional Documents
Culture Documents
a buffered solution and contains Formaldehyde as a
fixative agent. It is provided as a 10X concentrate. A
working solution (1X) of this concentrate is to be
ReaFix Fixative Solution used for the fixation of cell preparations before
10X Concentrate analysis by flow cytometry.
5. PRECAUTIONS 2. Mandy FF, Brando B. Enumeration of
Absolute Cell Counts Using
1. The ReaFix Fixative Solution contains
Immunophenotypic Techniques. Current
Formaldehyde. Formaldehyde is a potential
Protocols in Cytometry (2000) 6.8.16.8.26.
carcinogen and causes irritation to the eyes and
skin. Avoid inhalation, contact with eyes, skin and
3. Bossuyt X, Marti GE, Fleisher TA:
clothing. Wear suitable protective clothing. Never
Comparative analysis of whole blood lysis
pipette by mouth; if swallowed, seek medical
methods for flow cytometry. Cytometry
attention immediately.
30:124–133, 1997.
2. All blood specimens are considered biohazards.
Handle them as if they are capable of transmitting 4. Dressler, L.G., "Specimen handling, storage,
infection and dispose off with proper precautions and preparation", 1997, Curr. Protocols
in accordance with governmental regulations. Cytometry, Chapter 5, 5.0.1 5.2.15.
3. The addition of precise volume of deionized
7. WARRANTY
water is critical to obtain correct concentration of
the 1X working solution. Use a calibrated pipette The product is warranted only to conform to
and operate according to the manufacturer’s the quantity and contents stated on the label at the
instructions. time of delivery to the customer. There are no
4. The use of temperatures, incubation and vortexing warranties, expressed or implied, that extend beyond
times other than those specified in the the description on the label of the product.
corresponding product inserts may give erroneous ReaMetrix’ sole liability is limited to replacement of
results. the products. ReaMetrix is not liable for property
damage, personal injury, or economic loss caused by
5. The ReaFix Fixative Solution contains
the product.
Formaldehyde which is highly volatile. Do not
leave the lid in a loose or open position. The lid
CUSTOMER SUPPORT INFORMATION
should be tightly sealed after use.
6. Avoid microbial contamination of the reagent or ReaMetrix India Pvt. Ltd.
erroneous results may occur. 50B, II Phase, Peenya Industrial Area
Peenya, Bangalore560058, India
7. Results obtained with flow cytometry may be
Ph: 918028378693/5,
erroneous if the instrument is misaligned,
Fax: 918041172451
compensation spillovers are not correctly
Email: info@reametrix.com
compensated or the gate is improperly set.
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8. Reproducible results will be obtained as long as
Rev No. 1.0, 27Apr09
the procedure used is in accordance with this
package insert and with good laboratory practice
guidelines.
6. REFERENCES