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Experiment # 03
Objective of Experiment;
To find out the Reducing Sugar of Final Molasses.
Apparatus;
Weighing Balance, Hot Plate, Pipette, Burette
Procedure;
1) First of all weight accurately 4 g of final molasses.
2) Transfer to 250ml flask with some hot water.
3) Cool to room temperature by placing it in a cold water bath or streaming of running water make up
the volume.
MUHAMMAD MUDASSAR Page 4
4) Warm the contents of the flask to 40
o
C in a hot water bath.
5) Add 1g of potassium oxalate to precipitate the calcium from the solution.
6) Filter discarding first 25 ml of filtrate.
7) Pipette 50 ml of filtrate into a 100 ml flask dilute to volume and mix it.
8) Prepare Fehlings solution by adding 5 ml Fehling solution A and 5 ml Fehling solution B in
Erlenmeyer flask.
9) Pour some distile water into Fehling solution.
10) Add four drops of methylene blue indicator.
11) Heat it for 10 minutes and add filtrate into it until its blue color change into Bricks Red color.
12) Total ml of filtrate use to convert blue color into Bricks Red color is our reading.
Observation & Calculation;
From table 13 (UNIFORM METHOD OF CHEMICAL CONTROL) under 0.5 g sucrose / 100 ml.
Experiment # 04
Objective of Experiment;
To find out the Reducing Sugar of Final Molasses.
Apparatus;
Weighing Balance, Hot Plate, Pipette, Burette
Procedure;
1) First of all weight accurately 4 g of final molasses.
2) Transfer to 250ml flask with some hot water.
3) Cool to room temperature by placing it in a cold water bath or streaming of running water make up
the volume.
4) Warm the contents of the flask to 40
o
C in a hot water bath.
MUHAMMAD MUDASSAR Page 5
5) Add 1g of potassium oxalate to precipitate the calcium from the solution.
6) Pipette 50 ml of filtrate into a 200 ml volumetric flask.
7) Add 10 ml of 24.85% brix (6.34 N) hydrochloric flask.
8) Insert a thermometer and heat the flask and contents to 60
o
C in a water bath.
9) Agitate the flask during the initial 3 minutes of heating.
10) After total heating time of 10 minutes remove the flask and cool in a water bath to room temperature.
11) Dilute the solution to about 175 ml.
12) Add one drop of phenolphthalein in indicator and neutralize with 4N sodium hydroxide to the first
darkening of the solution.
13) Make up the volume i.e 200 ml.
14) Titrate against 10 ml of mixed Fehlings solution using methylene blue indicator.
Observation & Calculation;