BI 3.

KIM TRA CHT LNG GO TRNG

I. Xc nh m ca go trng
1. Nguyn tc
Tng t nh xc nh m ca thc. m chnh l lng nc t do c
trong nguyn liu. c nhiu cch xc nh m nh sy n khi lng khng i,
dng my o m.
Phng php sy: dng nhit lm bay hi nc trong nguyn liu n khi
lng khng i, tc l chnh lch khi lng gia 2 ln cn khng qu 5.10
-4
g.
Thc nghim ngi ta xc nh c cc thong s trong qu trnh sy la 105
o
C trong
3-4 gi, 130
o
C trong 45 pht.
Dng my o m: hot ng da vo dn in, nguyn liu c m cng
cao th kh nng n in cng cao v ngc li. Tuy nhin khc vi phng php
dng my o m ca thc, my o m ca go khng cn nghin m ch cn ht
vo my l my t ng o m ca go.
Trong bi thc hnh ny ta dng phng php dng my o m.

Hnh 3.1 My o m
2. Dng c
My o m
Cc 50ml ng mu
3. Cch tin hnh
Bc 1: khi ng my. Nhn cng tc ngun
Bc 2: iu chnh thng s my v nguyn liu cn o. C th y ta a v thng
s l Rice tc l o go.
Bc 3: b mu vo cc ng mu
Bc 4: Nhn ch MEASURE trn my, i xut hin ch pour nhp nhy th bt u
cho mu vo o.
Bc 5: Ta ly cc ng mu t t ln trn phm chp gia thn my nhm mc
ch ht phn b u v tng tnh ri ca ht (my o m dng ht hot ng da
vo dn in ca ht, ht m cng cao th dn in cng cao, ngoi ra tan ri
ca khi ht cn nh hng n kt qu o m ca my. Chnh v vy m cn ht t
t ln trn phn chp gia thn my nhm mc ch tng tnh tang ri v lm ht phn
b u)
Bc 6: Khi cho ht vo ta thy ch M nhp nhy phn cui gc pha tay phi ca
my, lc ny my ang o, ta i mt lc sau my s hin kt qu ra.
Bc 7: sau khi ht ra tin hnh lm li t bc 3. o t nht 3 ln. sau khi o xong
nhn nt ngun tc my.
4. Kt qu v nhn xt
Ln 1: 13,5%
Ln 2: 13,6%
Ln 3: 13,7%
Vy m trung bnh ca thc l
W% =

Kt qu o m bng my thng cho ta kt qu nhanh v chnh xc hn phng
php sy v phng php cm quan.
Tuy nhin o bng my cn phi v sinh my thng xuyn v lu ngy bi bn
s bm vo u o lm hn ch kh nng dn in nn dn n sai s.
Khi o cn thc hin ng thao tc, v khi mu vo my ta nhanh hay
chm th s ra kt qu khc nhau, chnh v vy cn cho mu vo t t c kt qu
chnh xc nht.
II. Chiu di ht go
1. Nguyn tc
Dng thc o chiu di ca 50 ht go to chc, khng h hng hay b sc m,
mu trng t nhin.
Xc nh chiu di ta dng thc kp. sau khi o kch thc ca 50 ht go t yu
cu ta tin hnh tnh gi tr trung bnh s ra c chiu di ht.
2. Dng c
Thc kp
Khy inox
3. Cch tin hnh.
Bc 1 :La ht go t yu cu nh to chc, mu trng t nhin khng h hng hay
sc m.
Bc 2:Dng thc kp o kch thc tng ht thc tch v, ta o kch thc ca
50 ht.

Hnh 3.2 Thc kp o go
Bc 2: Ghi li kt qu v tnh trung bnh.
4. Kt qu v nhn xt
Chiu di ca 50 ht thc. n v cm
1 0.63 11 0.71 21 0.70 31 0,67 41 0.66
2 0.65 12 0.66 22 0.66 32 0,72 42 0.70
3 0.68 13 0.62 23 0.61 33 0.71 43 0.68
4 0.61 14 0.60 24 0.66 34 0.63 44 0.68
5 0.65 15 0.62 25 0.70 35 0.69 45 0.65
6 0.61 16 0.64 26 0.63 36 0.62 46 0.60
7 0.62 17 0.65 27 0.62 37 0.63 47 0.61
8 0.71 18 0.60 28 0.65 38 0.64 48 0.61
9 0.66 19 0.61 29 0.66 39 0.72 49 0.63
10 0.63 20 0.62 30 0.74 40 0.61 50 0.60

Trnh trung bnh chiu di 50 ht ta c kt qu l 0,65cm = 6,5mm
Sau khi tnh trung bnh ta c chiu di ht l 6,5 mm, trong trnh o ta cn oc
thc kp thng mt, cn o nhng ht go t yu cu.
Trong qu trnh o ta nhn thy chiu di cc ht go dao ng trong khon 6 7mm.
v kch thc chng kh ng u nhau.
III. Xc nh t l tm
1. Nguyn tc
Ta dng my tch tm tch cc ht tm ra, sau cn lng ht tm v em chia
cho khi lng mu ta c t l tm.
My tch tm hot ng da vo nguyn tc sau:
Khi thng my quay cc ht tm c kch thc nh hn cc g trn thng th s lt
vo trong g, khi thng quay s a cc ht tm ny ln cao v ri xung phu hng.
Cc ht nguyn vn c kch thc ln hn cc g s c a ra ngoi v hng vo
mng cha.

Hnh 3.3 My tch tm

2. Dng c
My tch tm
Khay inox
Cc ng mu
3. Cch tin hnh
Bc 1: cn khong 200g mu
Bc 2: khi ng my tch tm, khi ny thng quay s quay u quanh trc.
Bc 3: ta cho mu vo phu nhp liu lc ny cc ht tm s c a ln cao tch ra
khi mu v ri vo phu cha.
Bc 4: sau khi lng ht nguyn vn vo ht mng cha bn ngoi ta tip tc thu hi
v cho tr li vo phu nhp liu tin hnh tch tm ln na, ta lm nh vy khong
3 ln.
Bc 5: sau khi kt thc qu trnh ta thu hi ht lng ht nguyn vn vo mng cha,
t xoay ngc phu ng tm ly tm ra ngoi.
Bc 6: em cn lng tm v tnh t l lng tm.

4. Kt qu v nhn xt
m
mu
= 200,06 g
m
tm
= 37,76 g
Phu nhp liu
Thng quay
Phu hng tm
Khuy hng mu
nguyn vn
T l tm =

Nhn xt
Trong qu trnh tch tm ta khng th tch hon ton lng tm ra khi mu, ngoi ta
trong qu trnh my hot ng c th c cc ht nguyn vn b gy v dn n sai s
cho kt qu sau cng.
IV. Xc nh phn trm cc loi ht trong go trng
1. Nguyn tc
Chn cc loi ht: bc bng, ht bin vng, ht xanh non, ht h hng, ht , ht ln
loi c trong mu phn tch. T tnh ra % cc loi ht.
Cc loi ht Khi nim
Ht vng Ht go c mt phn hoc ton b ni nh mu vng
Ht bc phn Ht go c b mt ht c mu trng c
Ht b h hng Ht go b gim cht lng r rt do m, cn trng
su b, bnh ph hoi hoc do cc nguyn nhn khc.
Ht xanh non Ht go cha chn hoc pht trin cha hon chnh
Ht Ht go c lp mu din tch b mt ca ht
Ht go xt di Ht go cn lp cm din tch b mt ca ht

2. Nguyn liu v dng c
- Nguyn liu: Go trng

Hnh 3.4 Go trng nguyn liu

- Dng c:
Cn phn tch
Khay nhm
3. Cch tin hnh
Cn 100g go trng dn u trn mt khay. Nht cc ht ni trn thnh tng phn ring
bit vo cc ng mu. Cn ring tng loi v t suy ra kt qu % khi lng cho
tng ch tiu cn xc nh.
4. Kt qu v nhn xt
Khi lng ht bc phn: 9,61g
Khi lng ht vng: 0,25g
% ht bc phn =

% ht vng =

Nhn xt:
- Phn trm khi lng ht bc phn l 9,61%. Phn trm ht vng l 0,25%. Phn
trm go cn li t cht lng l 90,14%.
- Cho thy go trng c mua c cht lng tt. Do go trng c xt trng v
ty trng t trc nn , xanh non khng c trong go thnh phm.
- Go trng hon ton khng c mi l, khng c cc ht b h hng do cn trng, su
b hay bt k nguyn nhn khc.
Hnh 3.6 Ht bc phn
Hnh 3.5 Ht vng
V. Kim tra tp cht c trong go trng
1. Nguyn tc
Kim tra xem c tp cht ln vo trong go hay khng. Tp cht c th l ct, sn, v
tru cn st li hay l thc ln vo trong go.
2. Nguyn liu v dng c
Nguyn liu: 200g go trng
Dng c:
Khay inox
Cc thy tinh 250ml
3. Cch tin hnh
Tri u 200g go trng ra khay inox. Kim tra xem c ln tp cht vo trong go hay
khng.
4. Kt qu v nhn xt
Go trng c kim tra l loi go c cht lng tt nn khng c tp cht ln vo
trong go.
VI. Xc nh hm lng tinh bt c trong go trng
1. Nguyn tc
Thy phn tinh bt bng HCl sau xc nh hm lng ng tng bng phng
php Bectrand.
Glucid kh Cu(OH)
2
mi trng kim mnh, to kt ta di dng Cu
2
O mu
gch. S lng Cu
2
O tng ng vi s lng glucid.
RCHO + 2Cu(OH)
2
RCOOH + Cu
2
O + 2H
2
O
Cu
2
O c tnh cht kh, tc dng vi Fe(III) lm cho mui ny chuyn sang dng Fe(II)
mi trng acid.
Cu
2
O + Fe
2
(SO
4
)
3
+ H
2
SO
4
2CuSO
4
+ H
2
O + 2FeSO
4

FeSO
4
c tnh kh, tc dng vi KMnO
4
. Do , c th dng KMnO
4
chun
FeSO
4
mi trng acid.
FeSO
4
+ 8H
2
SO
4
+ 2KMnO
4
K
2
SO
4
+ 2MnSO
4
+ 5Fe
2
(SO
4
)
3
+ 8H
2
O
T s ml KMnO
4
0,1N dng chun FeSO
4
hnh thnh, tra bng c s mg
ng glucoza, maltoza, lactoza hoc saccaroza nhn vi h thng pha long ta c
hm lng ng trong 1g bt go.

2. Nguyn liu, dng c, thit b v ha cht
- Nguyn liu: go trng
- Dng c, thit b:
STT Tn dng c Quy cch /v tnh S lng Ghi ch
1 Cc thy tinh 250ml ci 01
2 Cc thy tinh 100ml ci 01
3 Bnh tam gic 250ml ci 02
4 Phu ci 01
5 Buret nu 25ml ci 01
6 Pipet thng
10ml
5ml
ci 01/mi loi
7 Bnh nh mc 250ml ci 01

STT Tn thit b Quy cch /v tnh S lng Ghi ch
1 My xay ci 01
2 Bp in ci 01
- Ha cht
STT Tn ha cht Quy cch Ghi ch
1 NaOH 20%
2 Phenaltalein 1%
3 Fe
2
(SO
4
)
3

4 KMnO
4
0,1N
5 Fehling A
6 Fehling B
7 HCl m c


3. Cch tin hnh































Go trng
Nghin nhuyn
Cn 1g go nhuyn vo
bnh tam gic 250ml
100ml nc ct
Cho vo 4ml HCl m
c
Thy phn trong 1h
Lm ngui, trung ha
bng NaOH 20%
nh mc ti vch
250ml
Ht 5ml mu cho vo
bnh tam gic
Cho vo 10ml Fehling
B
Cho vo 10ml Fehling
A
un si 3 pht
Lc ly kt ta
Ha tan kt ta bng
Fe
2
(SO
4
)
3

Chun bng KMnO
4

0,1N
Mt s hnh nh tin hnh th nghim

























Hnh 3.7 un cch thy mu
Hnh 3.8 Trung ha, nh
mc mu
Hnh 3.9 Sau khi cho Fehling A
v Fehling B
Hnh 3.10 un trn bp
in
Hnh 3.11 Kt ta thu c
4. Kt qu v nhn xt
Hm lng tinh bt trong go trng:
X =
.
.100.0, 9
.1000.
dm
m
aV
m V

Trong :
a khi lng ng nghch o (mg)
V
dm
: th tch nh mc
m: khi lng mu (g)
V
m
: th tch mu.
0,9 l h s chuyn i t glucose sang tinh bt
Kt qu:
Th tch KMnO
4
tiu tn:
V
1
= 1,8 ml
V
2
= 1,7 ml

= 1,75 ml
Tin hnh tra bng xc nh hm lng ng nghch o.
Do th tch KMnO
4
tiu tn l 1,75 ml nm ngoi bng tra hm lng ng nghch
o. Cho nn khng th a ra kt qu hm lng tinh bt trong go trng.
Th tch KMnO
4
tiu tn qu thp c th do hm lng ng nghch o qu
t. C th tin hnh th nghim li ht lng mu cao hn 5ml. c th cho
kt qu sau cng.
Nhng do thi gian thc hin th nghim c hn nn khng th tin hnh li th
nghim.
Kt lun:
Khng a ra c kt qu th nghim sau cng. Nguyn nhn c th do:
- Qu trnh lc tch kt ta b tht thot nhiu kt ta, kt ta cn bm trn b mt
dng c th nghim.
- Dung dch Fe
2
(SO
4
)
3
cha ha tan hon ton lng kt ta bm trn giy lc.
- Ha cht s dng khng tinh khit, nng ha cht cha c hiu chnh.
- Dng c th nghim c th b bm bn.
- Kh nng nhn bit im dng chun cha chnh xc.