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The process begins by extracting DNA from the cells in a sample of blood,
saliva, semen, or other appropriate fluid or tissue.
An analysis is performed by a cut into the DNA into fragments which are
separated into bands. The bands of DNA are transferred via a technique called
Southern blotting. This is treated with a radioactively-labeled DNA probe which
binds to certain and specific DNA sequences on the membrane. The excess
DNA probe is washed off. An X-ray film placed next to the nylon membrane
detects the radioactive pattern. This film is then developed to make a visible
pattern of bands called DNA fingerprinting.
One of the most modern and widely accepted methods for producing DNA
fingerprints in criminal cases, is that of polymerase chain reaction (PCR). PCR
involves the amplification of specific regions of DNA that are known to be
. PCR is by far the most common method for presenting DNA evidence in a
forensic context. .
One of the main problems with the process of DNA fingerprinting is that the
sample can be easily ruined. The tiniest pieces of genetic junk can contaminate
DNA samples, causing them to be useless. Although DNA fingerprinting requires
a good sample to work with, this problem can be solved by using the newer
technique called PCR. PCR can use extremely small samples of DNA and
produce a much faster result. But this also means the DNA samples that PCR
uses are even more likely to be contaminated because of their size, as it is
harder to find a small sample with hardly any contamination. Another limitation of
fingerprinting is that the procedure is so complex and hard to read the DNA
patterns, that sometimes the juror finds the evidence almost invisible.
Although DNA Fingerprinting is a highly advanced process, there are still some
things that it is unable to do. In dogs for example, a fingerprint does not make it
possible to determine if the animal is a carrier of a disease causing allele. Also,
a DNA fingerprint is unable to show a crossbreed in animals. This is because
second or third generation crosses cannot be seen by working backwards in a
pedigree. It may soon become possible to discover the crossbreed of dogs,
although right now this is not possible.
Technique