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21 Magsumbol
22 Mandilag
23 Marcelo
24Mendoza
Objectives
To isolate potential microorganisms as producers of
What is molds?
Molds are fungi that grows in the form of multi cellular
Importance of molds
Molds play important roles in biotechnology and food
Importance of molds.
Some of the products of metabolism have great
importance.
Ex. The mold called Rhizopus produces fumaric acid
which can be used In the production of the drug
cortisone.
Other molds can also produce alcohol, citric acid and
oxalic acid.
What is antibiotics?
Antibiotics are a very important part of modern
medicine.
Antibiotics only work only against infections that are
caused by bacteria. These drugs are not effective at all
against most viral infections.
Some antibiotics are effective against only certain
types of bacteria; others can effectively fight a wide
range of bacteria.
Common
soil
molds
2 most common soil molds are Aspergillus and
Penicillium
Penicillium chrysogenum
The source for penicillin (the first antibiotic.)
Penicillin works against gram-positive bacteria, such
Penicillium
chrysogenum
Fungal
Isolates
C. albicans
E. coli
P.
aeruginos
a
S.
aureus
A. niger
(10 mm)
(15 mm)
(7 mm)
(16 mm)
A. flavus
(12 mm)
(8 mm)
No effect
(12 mm)
A.
fumigatus
(11 mm)
No effect
(13 mm)
(14 mm)
Aspergillus niger
Aspergillus fumigatus
Aspergillus flavus
METHODOLOGY
Weigh
Dilute
Inoculate
Incubate
Identify
Inoculate
Incubate
Examine
RESULTS
Botanical
A
C
B
Zone of
Inhibition
Test
organism
Gram
T.O.
5.50mm
P. aeruginosa
6.30mm
S. aureus
4.50mm
P. aeruginosa
Botanical
D
E
F
Zone of
Inhibition
Test
organism
Gram
T.O.
8.50mm
P. aeruginosa
11.80mm
P. aeruginosa
12.25mm
P. aeruginosa
Zone of
Inhibition
Test
organism
Gram
T.O.
26.25mm
P. aeruginosa
15.95mm
P. aeruginosa
16.60mm
P. aeruginosa
Sludge
Zone of
Inhibition
23.50mm
P. aeruginosa
16.70mm
P. aeruginosa
Summary of Results
Source
Zone of
Inhibition
Test Organism
Gram Stain
T.O.
Botanical
5.50mm
P. aeruginosa
Botanical
6.30mm
S. aureus
Botanical
4.50mm
P. aeruginosa
Botanical
8.50mm
P. aeruginosa
Botanical
11.80mm
P. aeruginosa
Botanical
12.25mm
P. aeruginosa
FLR
26.25mm
P. aeruginosa
FLR
15.95mm
P. aeruginosa
FLR
16.60mm
P. aeruginosa
Sludge
23.50mm
P. aeruginosa
Sludge
16.70mm
P. aeruginosa
CONCLUSION
The group therefore conclude that molds isolated from
References:
Penicillium chrysogenum (aka P. notatum), the natural source for the wonder
drug penicillin, the first antibiotic. Tom Volk's Fungus of the Month for
November 2003. [http://botit.botany.wisc.edu/toms_fungi/nov2003.html]
Date retrieved 6 November 2014
Makut, M. D., & Owolewa, O. A. (2011). Antibiotic-Producing Fungi Present in
the Soil Environment of Keffi Metropolis, Nasarawa State, Nigeria. Trakia
Journal of Sciences, 9(2), 33-39.