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Variability of 2 •The original name of the flow cytometry technology was pulse cytophotometry.
ReaMetrix’s Dry
Reagents •The first FACS instrument from Becton Dickinson came in 1974.
A summary table- 3-4 •According to a market research report, the global flow cytometry market in 2008 stood at $1.5 billion and
Flow systems is estimated to grow to $3.7 billion by 2015 with estimated growth rates of existing product. About 68
percent of the 2008 product revenue has been from instruments, and reagents make up 32 percent of the
revenue.
To know the answers for
•The first fluorescence-based flow cytometry device (ICP 11) was developed in 1968 by Wolfgang Göhde
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from the University of Münster, Germany (patent no. DE1815352)
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1
Types of Flow Cytometers
A brief of the commonly used flow Beckman Coulter. EPICS-XL is a single pact and portable and facilitates quick
cytometers based on microsphere laser four color, six parametric open results at the point-of–care . It is a
beads and the volumetric method are system. It utilizes Flow-Count Liquid closed system and used for CD4 enu-
mentioned below. beads with premixed monoclonal anti- meration. The system requires minimal
body combinations of three or four operational training. It requires no
FACSCount microbead-based sys-
antibodies. calibration and sample set up. It uses
tem: FACSCount system is the prod-
light scattering parameters and not
uct of Becton Dickinson Biosciences. Guava EasyCD4 volumetric system:
the fluorescence dyes to distinguish
It is the only available microbead- The Guava EasyCD4 system (Guava
lymphocytes from white blood cells. It
based single-platform instrument Technologies) is a desktop modified flow
is a mobile machine and can work on
that is designed specifically for enu- cytometry based on a micro capillary
battery back-up, however the samples
merating the absolute CD4+, CD8+ volumetric cytometry technology. Its
have to be processed in a very short
and CD3+ T-cell counts in no-lyse no- novel feature is that, it does not require
time (4 hours of collection).
wash whole blood. It is a closed sys- sheath fluid. The instrument uses two
tem. color monoclonal antibody combination LabNow CD4 system:
reagents and a volumetric control sys-
FACScan/FACSCalibur instruments This portable system was developed by
tem that allows a precise count of cell
with TruCount microbead-based LabNow for rapid CD4 enumeration.
numbers and fluid volume, and is regu-
system: FACScan/FACSCalibur sys- The test is conducted in a ‘single time
lated by a variable-speed fluid (stepper
tems are the product of Becton Dick- use’ disposable microfluidics biochip in
motor syringe pump).
inson Biosciences. FACScan is a single conjunction with a portable analyzer.
laser three color five parametric open Partec CyFlow counter (volumetric The system facilitates minimal opera-
system and FACSCalibur is a dual system): CyFlow is a desktop single- tor interference that requires a single
laser four color six parametric open platform volumetric cytometry technol- drop of blood to be placed on to the
system. It utilizes TruCount absolute ogy made by Partec, Germany. The card for CD4 analysis.
count tubes (lyophilized pellet of Partec flow cytometer is equipped with
beads in a test tube) or Liquid Tru- two sensing electrodes for a true volu-
Count beads with premixed mono- metric absolute counting with up to five
clonal antibody combinations of three light sources. It supports real time
or four antibodies to enumerate data acquisition. CyFlow counter can be
CD4+, CD8+ and CD3+ T-cell counts used as a mobile system that can run on
and/or Absolute lymphocyte counts. car batteries.
EPICS-XL Instrument with Flow PointCARE system: This new flow sys-
count microbead-based system: tem has been developed by Pointcare
EPICS-XL is an open system from Technologies. The system is very com-
System Features
BD FACSCalibur Open system with optional MultiSet software for automated gating of some assays.
Dual laser (488 nm and optional 635 nm), 2 scatter detectors (FSC/SSC).
Continued on P4...
3
Summary of Flow Cytometric Systems (continued from p3)
System Features
Dual Laser (488, 635 nm), 5 color, two scatter detectors (FSC,SSC).
20 bit system with digital post acquisition compensation.
CoulterPrep Station automation option available for sample preparation, together with
multi-carousel loader.
Volumetric system.
16 bit system.
Automated gating.
system
Dedicated for CD4 and CD4%.
Dual Laser (488, 635 nm), 4 color, two scatter detectors (FSC,SSC).
system
24 bit system with digital post acquisition compensation.
Gainless operation - factory set gains, enabling simple user interaction with the instru-
ment.
Accommodates variety of inputs - 96 well plate, FACSTubes, eppendorfs.
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