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Parental!
Inheritance!
F1 testcross!
Variation !
Genetics is the
study of
inheritance and
variation!
Expected proportions of !
F1 testcross progeny!
2!
Dihybrid cross!
a) Punnett square!
b) Branching
combinations!
c) Multiplicative
rule!
3!
Inheritance!
Variation !
Test cross!
4!
% recombinants is
proportional to !
the distance between !
gene locations (locus, loci) !
on a chromosome!
G1!
Mapping functions
(p=1/2 (1-e-2d)
d=crossover frequency
6!
Interference = 1- coefficient of
coincidence measures the
difference between expected
double crossover and observed
double crossover.
1. Organize Data!
vbp
1779
VBP
vbp
1654
1779
Vbp
252
vBP
241
VbP
131
vBp
118
vbP
13
vbP
13
VBp
VBp
VBP
1654
A. Parentals!
B. Double recombinants!
!
C. Order ?!
V!
7!
P!
B!
1654
1779
vPB
Vpb
241
252
Region VP
= 241 + 252 + 13 + 9
515.000 /4197
0.123
VPb
vpB
131
118
Region PB
= 131 + 118 + 13 + 9
271.000 /4197
0.065
v Pb
VpB
13
9
Region VB
4197
V! 12.3 m.u.!
P! 6.5 cM! B!
8!
F
f
A
a
Hl!
hl!
Parental F A Hl
45!
recombinant
recombinant
Parental f a hl
9!
10!
11
Incomplete dominance:
the phenotype of the heterozygote
is intermediate, its expression
somewhere between the two
homozygous genotypes.
Reserve the term codominance
for a categorical intermediate-
Codominant Phenotypes:!
!
All phenotypes indicate unique
phenotypes!
12
R r x R r!
RR
1 :
Rr
2
:
rr!
1!
r!
R!
R R!
R r!
r!
R r!
r r!
13
0.75 A_
0.25 BB
0.5Bb
0.25bb
F 2!
0.25 BB
0.5Bb
0.25 aa
0.25bb
14
Incomplete dominance!
Co-dominance!
Three or more alleles of a single gene!
Recessive lethal alleles!
15!
A!
A!
B!
B!
16!
B!
B!
B!
A!
B!
A!
17!
IA IA
IA IB
A type
IB IB!
AB type
B type !
1
!
A!
1 !
A!
A!
B! A!
B!
B!
B!
B!
B!
A!
A!
A!
B!
A!
B!
18!
IA i
ii
Type O
Type A
IA IB
IB i!
Type AB
Type B
A!
B! A!
B!
A!
A!
B!
B!
B!
A!
B!
A!
A!
B!
B!
A!
19!
native antibodies !
against foreign (non native) antigens !
!
Phenotype!
!
initiate !anti A or anti B!
!
initiate !anti B!
initiate anti A!
initiate !neither A nor B!
20!
i!
I A!
Expected Ratios ?!
i!
ii!
i IA!
I B!
i IB
I B IA!
ii
- type O!
i IA type A!
i IB - type B!
IB IA - type AB!
21!
22!
23!
!
Dominant lethal alleles exist, !
Why are they not segregating in high frequencies in most populations ?!
Why are they seen at all ? late onset lethal diseases e.g.
Huntingtons, Crones.!
!
Conditional lethal: environmental conditions trigger lethality-!
e.g. temperature sensitive lethals or conditional mutants!
!
Lethal alleles may also have partial dominance: !
e.g.:, a test cross yL x yy does not give a 1:1 ratio but repeatedly shows
70 / 30 or some ratio other than 50% each. This is called a sub-lethal
allele.!
24!
25!
28!
29!
(4) Chromosome Structure: Gene Duplications: Intergenic and tandem duplications have
functional and evolutionary significance. For example a duplication explains the difference in
color vision of new and old world primates. Note that green (G) and red (R) pigments are
polymorphic alleles in new world primates (NWMonkeys) but blue (B) is monomorphic.
30!
Recessive Epistasis 9: 3: 4!
31!
33!
Many forms of
epistasis
34!