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(1)
Time-of-entry
mapping ( from 2)
Science 28 November
2003:vol. 302 no. 5650
1488
Within a cell:
8
Getting a gene to express mRNA means there has to
be:
(1)Environmental conditions require specific mRNA
and in turn, protein expression.
(2) There has to be promoter that can be bound by the
RNA polymerase holoenzyme.
(3) There has to be a clear path to the start codon, a
path that is not blocked otherwise RNA polymerase will
stall and fall off the DNA template.
(4) There has to be an (unblocked) downstream
functional gene
Repressible A
repressor slows down
or stops the
expression of a gene
10
11
12
repressor
1+ site
promoter operator
13
14
Negative Control
Glucose absent
15
Constitutive,
16
17
I-
18
19
- Gal
Genotype
Noninduced
Induced
Permease
Permease
Noninduced
Induced
Conclusion
I+ Z+Y+
I- Z+Y+
I- Z+Y+
/ F I+
I+ is dominant to I-,
I is trans acting
and .
20
21
22
23
25
26
27
Why negative control - always on
28
I+O+Z+Y+
29
30
Negative control
Default state is on,
Inducible
An inducer
molecule activates
a gene
De-represses a
gene, turns a gene
on or, promotes
transcription
Repressible
A repressor slows
down or stops the
expression of a
gene
Positive control
Default state is off-
31
32
Figure 10-13
(1) Co- repressor (does not bind
33
34
Negative control
Default state is on,
Inducible
An inducer
molecule activates a
gene
De-represses a
gene, turns a gene
on or, promotes
transcription
Repressible
A repressor slows
down or stops the
expression of a
gene
Positive control
Default state is off-
INDUCIBLE: the
inducer removes the
blocking regulator
(repressor), allowing
expression (the
default state - on) Lac
control
Positive
Default state is off
cAMP
produced,
switch on
Negative
Default state is on
35
36
E coli
chromosome
lac operon
trp R
trp operon
trp R
37
Negative control
Default state is on,
Inducible
An inducer and /or
activator
De-represses a
gene, turns a gene
on or, promotes
transcription
Repressible
A co repressor acts
with a repressor to
slows down or stop
the expression of a
gene
Positive control
Default state is off
38
Key is
sufficient
tryptophan
available at the 2
trp codons in the
leader region (14
AA) ?
Not present-stall
Present- forms
the terminator