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During replication E coli- SOS repair use DNA polymerase II, IV,
V (eukaryotes - translesion repair). These are low - fidelity (error
prone or high mutation) systems involving recombination-like
repair, that: 1) puts an undamaged strand near the damaged one;
2) excise the damaged part , then; 3) copy undamaged.
unwind
UV induced
Rec A binds
SSB, binds
single and
double strand
DNA.
Inducessignals
Bypass Poly
V-PCNA
Recognize
TFIIH 10 subunits
Large number of
other proteins act to
remove damaged
base + 30 others..
Induced
mutations
through the action of
mutagens.
High energy like X rays
cause double strand
breaks among various
other mutations
Measured as % death of F2
(hemizygous) males carrying
irradiated , P, X chromosomes
through marked , heterozygous F1
females
4
Cause:
Induced
mutations
through the action of
mutagens.
High energy like X rays
cause double strand
breaks among various
other mutations
Measured as % death of
(hemizygous) males
Error-prone
nonhomologous end
joining repairs double
strand breaks causes minor
mutations G1, (S or G2):
Bind Ku80- Ku 70
Trim
Ligate
Replication fork
assembles on
Displacement
loop
Meiotic Recombination
Double-strand CUTS (SpoII)
initiate meiotic recombination,
binds to free 5 ends and protects
from free binding
(1) Attracts proteins to trim 5 end
and unwind,
(2) Rad51attaches to the 3 end.
(3) Coats strand and associates
with Dmc1-Rad.
(4) Rad51-Dmc-1 scans for
complementary sequence in a
homologous chromosome.
(5) Strand invasion and chiasma
formation - D loop strand
extension and ligation