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2"
3"
1929
Cornell University
Ithaca, New York
George Beadle
Pudgie
4"
B. Mclintock
discovered
transposable
elements while
studying
unstable genes
that regulate the
color of the
aleurone layer!
Figure 14-3"
"
6"
c) Ds moves Ds maps to C, !
Color is blocked Ds inserts
into C gene, unless Ac is
present (sometimes
mapped), it stays."
d) Unstable : Ds transposes"
Color is expressed in some"
aleurone cells. "
But same for starchy/ waxy in
unstable color lineDs moves
and leaves a copy behind!
"
8"
8"
"
P element inverted
repeats are 32
nucleotides long,
specific for each
family and when
they insert they
initiate a repair
8bp inverted
repeats long.!
12"
Flies from strains captured before 1950 do not have P elements, wild
populations vary in the number of P elements, some close relatives of Drosophila
melanogaster have no P elements , distant fly relatives have some."
P elements were reported in in fly stocks during the 1960's. They were not
observed in any fly stocks isolated before 1950. "
What made them noticeable was that two mating type strains were first seen
over a 10 15 year period in recently isolated fly strains and in lab strains
exchanged between Australia and United States and used in both countries.
Crosses between two strains produced dysgenic M-P hybrids that had
frequent mutations, chromosome breakage and sterility. One strain named the P
for paternal P and M for maternal. Populations that possess P elements
have mechanisms to regulate their movement, more specifically, a repressor
in a P cytotype represses P element movement. The M cytotype permits it. This
repressor appear to target mRNA for destruction by a small RNAs - RNA
interference. "
female"
M"
P"
male"
M"
P"
"
normal" normal
normal"
dysgenic!
dysgenic" normal"
13"
Figure 14-11"
15"
16"
A retrotransposon is
transposed through an
RNA intermediate, using
reverse transcriptase/
integrase in pol. "
gag encodes a structural
protein of the virus-like
capsule. "
LTR retrotransposons "
are flanked with "
Long Terminal Repeats. !
17"
http://www.nature.com/
scitable/topicpage/
transposons-the-jumpinggenes-518
18"
19
Eukaryotes
Structure!
Genes
Encoded!
Transposition!
Examples!
Cut &Paste!
Short terminal
Transposase
inverted repeats
gene!
characteristic of a
family. Target site
direct repeats!
Retrotransposons!
Long terminal
direct repeats.
Target site (short)
direct flanking
insertion repeats!
Through an
RNA
intermediate
using an
integrase to open
host DNA,
reverse
transcriptase to
insert DNA!
Reverse
transcriptase,
integrase (pol)
and a protein
(gag)!
Ty1 (yeast)!
Copia
(Drosophila)!
LINES , L1
or Alu in
humans!
20"
Transposition in Prokaryotes"
(1)Bacterial Insertion sequences, (IS) may encode a transposase (cut & paste)
only, they can move around the bacterial genome, and insertions are often
deleterious. These transposons also have characteristic, inverted terminal
repeats and direct target site duplications.
"
"
21"
http://
www.sci.sdsu.edu/
~smaloy/
MicrobialGenetics/
topics/transposons
Replicative
transposition of
Tn3"
http://www.sci.sdsu.edu/
~smaloy/MicrobialGenetics/
topics/transposons
Prokaryotes" Structure!
Genes
Encoded!
Transposition!
Examples!
IS elements!
!
!
!
!
"
Composite"
"
transposons!
Short terminal
Transposase
inverted repeats gene!
characteristic of
a family. Target
site direct
repeats!
!
Flanking IS
elements may be
in an inverted or
repeated
orientation!
"
No IS elements!
Characteristic
terminal
inverted repeat
sequences!
!
Replicative
mechanism!
!
Tn3!
Simple
transposons!
!
Transposase
gene &
Resolvase!
23"
24"
Any piece of
bacterial
genome or
plasmid could
have a
transposon"
25"
26"
23"