Professional Documents
Culture Documents
Used to treat
Deoxyribonuclease
Epidermal growth factor
Erythropoietin
Factor VIII
Fibroblast growth factor
Follicle stimulating hormone
Insulin
Growth factor -1
Interferon
Interferon
interferon
Lung surfactant protein
Serum albumin
Tissue plasminogen activator
cystic fibrosis
Ulcers
Anemia
Hemophilia
Ulcers
Infertility
Diabetes
Growth 'disorders'
Leukemia
Cancer , Aids
Cancer , Rheumatoid arthritis
Respiratory distress
Plasma supplement
Heart attack
Forward
Genetic
Analysis
homogentisate
dioxygenase
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and (5) you can use transposable
elements
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10
Injecting naked
double stranded DNA
or using a vector to
transport it.
Ectopic insertion random insertion
into a chromosome
during the pronucleus
stage
..(or mostly not),
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Possibly causing :
(1)Ideally, the transgene integrates
into a germ line chromosome ,
and is expressed.!
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1% recovery
5,000 copies
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Two possibilities,
(1) ectopic integration
into a chromosome
(position effect ,
insertion effect) ? or
(2) it may be
maintained as an
extrachromosal
array:
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Rosy is
dominant
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The bacteria Agrobacterium tumefaciens naturally transforms some
plant species when the T-DNA segment of the 200kb Ti plasmid
inserts into a chromosome. This ability is used to produce
recombinant plant cells.
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Inherited
knockout
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The SALK Homozygote T-DNA Collection http://signal.salk.edu/
PROJECT ABSTRACT NSF DBI- 0726408
This project specifically addresses an important aim of the 2010 project - to identify genetically
stable loss-of-function mutations in all Arabidopsis genes. Computational analysis of the
initial genome sequence of Arabidopsis thaliana in 2000 provided evidence for the existence of
approximately 25,500 genes. .The identification of T-DNA/transposon insertion mutations
in all Arabidopsis genes has been an on-going aim of worldwide functional genomics program for
example..
Alonso et al. 2003, Science 301Over
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Homologous
recombination
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