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L14 Mutation
Forward Genetics depends on phenotypic variation as an index of
genetic variation: its inheritance or transmission-genetic pattern; how
transmission is related to chromosome variation; how character and
phenotype differences depend on metabolic pathways involving several
genes that may or may not be functional; how they replicate, express
peptides and how this expression is regulated, but not source(s) of mutation .
Scale of mutation: this can range from a change in a single base (substitution),
through several bases (insertiondeletion), to a chromosome fragment.
Mutation rate is low What really governs mutation rate is the efficiency of
repair mechanisms. Regardless, the rate is never 0, some base changes (1) go
un-repaired, or, (2) repair mechanisms introduce a mutant variant.
If one of these (1 or 2) occurs in a germ cell (sperm,eggs) which happens to
segregate and produce an embryo, it will be passed on to descendent
generations.
M. Lynch.
2012.
Evolution of
the mutation
rate.
Trends in
Genetics. V
26 pp 345-362
Mutations are:
(1) potentially permanent (mutations in somatic cells), and
(2) possibly inherited changes in DNA sequence (mutations in germ cells).
Classify:
(A) spontaneous - occurring in the absence of known mutagens
or
(B) induced mutations - require an known agent that increases the
mutation rate significantly above the spontaneous rate.
Spontaneous mutations
Rare tautomers
Spontaneous mutations
Tautomeric or keto-enol,
amino - imino shifts
suggested by Watson &
Crick (1953), involve:
(1) the migration of a Hbond ,
(2) the switch of adjacent
single and double bonds
Depurination -covalent
bond between sugar and
purine is less stable than
sugar-pyrimidine.
= rare single base loss
Note this tautomeric shift must happen during the process, just
before/as DNA replicates.
An apurinic site - any complementary base could be substituted
during replication.
(1) Base selection if the correct base is not chosen, the polymerase site in the
holoenzyme will not be activated.
(2) Proof reading: 3-5 exonuclease activity (DNA pol III)
(3) Mismatch repair after replication, the wrong nucleotide will not generally
pair correctly, it causes a bulge in the helix, mismatch repair enzymes (Mut S, L
and MutH) scan for bulges, if found soon after replication, non-methylated
strands are repaired based on its methylated complement. See fig 15-26
If both strands are methylated, the mutation may be inherited (pp 536-538).
Base analogues (pp 526) cause transitions or transversions in the
first replication. E.g. 5 bromouracil
Intercalating agents (pp 528) cause indels -
insertions and deletions.
?conservative chemically similar neutral mutation
?non-conservative - chemically different
5 bromouracil is an analogue of
thymine
It forces a tautomeric
shift
10
11
substitutions in a nucleotide sequence that do not cause a change in
amino acid sequence.
12
13
14
16
17
Mutation,
1. Point mutations substitutions,
- synonymous
- missense mutations,
- nonsense mutations
- sense mutations
2. Point mutations -Frame shift mutation - indels
- insertion or deletion of nucleotides,
-insertion or loss of one or two nucleotides
18
19
A 3 base pair addition or deletion mutation
will add or delete one amino acid. In this case the effect
depends on the chemical similarity of the protein with or
without one of hundreds of amino acids. Any addition or
deletion not dividable by 3 will cause a frame shift.
G deletion
Mutation
DNA:
ATG CGA CCT CGA AGT GCT CTA
Protein
20
21
22
Human
Normal
Karyotype
Karyotype
from
cancer cell.
Note extra copies
lost copies
translocations
Duplications
A B
A B
C C
A B
24
E
D
F
E
G
F
E C D
H
G
E F
I
H
J
I
J
H
, Molecular
order (5- 3)
reflects their order
of expression.
26
27
1.Duplications
28
2. Deletions of genes or a large portion of its sequence
3. Inversions
4. Translocations
Many mutations occur in meiosis
29
Deletions:
A B
A B
1. Duplications
2. Deletions
3. Sequence inversions of many bases to several genes.
4. Translocations
30
A B
F E
A B
G F E
31
33
Recombinants are inviable, thus
1. Duplications
2. Deletions
3. Inversions
4. Translocations of chromosome segments
34
35
Reciprocal translocationsbreak and reciprocal fusion
of non-homologous
chromosomes.
Meiosis I:
Alternate segregation - both
pairs have a complete gene
set - fine
Adjacent segregation pattern
produces inviable gametes
Thus translocation
heterozygotes are semisterile
(fig. 16.30)