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Population
Genetics!
Hardy
Weinburg
Equilibrium, !
And Natural
Selection!
In domestic selection,
breeders and
agriculturalist determine
the fitness of individuals
by choosing them to
breed due to their
desirable characters.!
In natural populations,!
natural selection acts on traits
that are closely related to
fitness (affecting survival and
reproduction)!
3!
4!
5!
"
Classic Population Genetics predicting and explaining
changes in allele frequency in a population."
"
The study of the frequencies of different genotypes in
populations and the changes in those frequencies that result from
patterns of mating, natural selection, mutation, migration and
random chance. Griffiths et al. "
"
Study of allelic transmission and genotype variation within and
among populations, the units of microevolution.!
!
0.5!
A(p)!
!
0.5!
a(q)!
!
2!
0.25p
A(
p)!
0.5!
0.25pq!
!
0.25q2!
AA + Aa + aa =1
A=p
a=q
p2 + 2pq + q2 = 1
7!
p2 + 2pq + q2 = 1 !
count 3 genotypes if codominant, !
count the proportion of recessive phenotypes (q2) if fully dominant; !
& calculate q, p (1-q) and the expected genotype frequencies.!
Then consider "
Effects of population structure, size, migrants, predators and competitors!
17!
Eggs!
Sperm!
A!
(p = 0.7)!
18
A(p = 0.7)!
A(p = 0.7)!
Eggs!
Sperm!
AA!
(p2 = 0.49)!
19
Eggs!
Predicting genotype
diversity in a population.
Sperm!
A(p = 0.7)!
a(q = 0.3)!
A!
(p = 0.7)!
AA!
(p2 = 0.49)!
Aa!
(pq = 0.21)!
a!
(q = 0.3)!
Aa!
(pq = 0.21)!
aa!
(q2 = 0.09)!
20
p2
q2
+ 2pq +
=
Weinburg
1Hardy
!
Generalizations
In specifically a population
with genotypes in HW
equilibrium:!
!
(1) If there are equal allele
frequencies (0.5), then
heterozygotes are in the
highest frequency.!
(2) At unequal frequencies, a
high proportion of low
frequency alleles alleles are
in heterozygotes. For fully
dominant allele interactions,
recessives in heterozygotes
are hidden, carried, masked
or are not a direct target of
selection.
Selection
survivorship
fecundity!
Zygotes -> natural selection -> adults reproducing!
!
!
Assumptions!
The population is initially in Hardy-Weinburg equilibrium.
In this population the allele frequencies are 0.5 (A1) : 0.5(A2), thus:
q2= 0.25,
p2 =0.25 and
2pq = 0.5
19
Frequency
0.5!
Parental generation
at conception.
0.25!
0.25!
100 individuals!
Phenotypic value
Genotype
Zygote frequency
A1A1
p2
A1A2
2pq
A2A2
q2
20
Frequency
0.5!
Genotype
Zygote frequency
Relative fitness
Product
0.25!
Parental generation
at conception.
0.25!
Phenotypic value
A1A1
A1A2
p2
w11
p2w11
(0.25*1.0)
2pq
w12
2pqw12
(0.5*0)
A2A2
q2
w22
q2w22
(0.25 *0) !
0.5!
Frequency
22
0.25!
w =
Adult frequency
0.25!
Phenotypic value
A1A1
A1A2
Genotype
Zygote frequency
Relative fitness
Product
Parental generation
at conception.
p2
w11
p2w11
(0.25*1.0)
p2w11 / w
2pq
w12
2pqw12
(0.5*0)
2pqw12/ w
A2A2
q2
w22
q2w22
(0.25 *0) !
q2w22 / w
Directional
Selection on a fully dominant allele: Assume A2 allele is
dominant lethal.
wA1 A1 = 1, wA2 A1 = 0
wA2A2 = 0
Selection (2) !
Directional or elimination selection on a fully recessive allele in a
gene with 2 alleles A1 , A2, HWE , 100 individuals, p & q = 0.5"
Assume A2A2 fitness is 0, A1 is fully dominant over A2.
A1 = 0.5, A2= 0.5
w A1 A1 = 1,
Genotype
Zygote frequency
Relative fitness
w=
Product
Adult frequency
A1A1
w A2 A1 =1
w A2A2 = 0
A1A2
A2A2
q2 = 0.25
w22 = 0
q2w22 = 0
q2w22 / w
= (0.25*1.0)/0.75 = (0.5*1.0)/0.75!
75 adults left
0.33A1A1 (25) ,
23
1 " 0.5 %
0
q1 = $
= 0.33
'+
2 # 0.75 & 0.75
24
GENE
POOL
f(A1) = 0.66
(p)
f(A2)= 0.33
(q)
f(A1) = 0.66
(p)
0.44
f (A12) = p2
0.22
f(A1A2)= pq
f(A2)= 0.33
(q)
0.22
f(A1A2) = pq
0.11
f(A22) = q2
25
frequency dependent
frequency independent.
14
Darwinian Selection
(1)Variation
(2)Heredity
(3)Natural Selection
Frequency dependence
Nature 441:633-636
15
10
Variation in
color and
allele
pattern!
6!
Variation in
chromosome
inversions!
7!
Detecting
variation in
microsatellites"
8!
Asterisks mark
restriction haplotype
variation among 58
D. pseudoobscura!
chromosomes !
F0, 1 can be used to
estimate allele
frequencies!
9!