Code No: RR322304 Set No.

1
III B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007
BIOPROCESS ENGINEERING-II
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. What is meant by Multiphase bioreactors ? Explain in detail about any two Mul-
tiphase bioreactors ? [16]

2. Write short notes on: [8+8]

(a) One-parameter model

(b) Two-parameter model

3. Define non-mechanically agitated vessels? Explain the design and operation of such
a reactor? [16]

4. Write in detail about the methods employed in measuring the following process
variables. [8+8]

(a) Temperature
(b) Pressure.

5. (a) Discuss in detail about ON-Line Sensor.

(b) Discuss in detail about OFF-Line Analytical Methods [8+8]

6. What do you mean by a vector and explain its properties. [16]

7. Write Short Notes On: [16]

(a) Monoclonal antibodies.

(b) Immunobiological Regulators.
(c) Virus Vaccines.
(d) Hormones.

8. Discuss in detail about segregated kinetic models for growth and product formation.
[16]

⋆⋆⋆⋆⋆

1 of 1
Code No: RR322304 Set No. 2
III B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007
BIOPROCESS ENGINEERING-II
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Define Fed batch culture ? What are the different strategies employed in Fed batch
systems and discuss about them ? [16]

2. Explain in detail about models for Non-Ideal reactors ? [16]

3. What is Cyclic fed batch culture ? What area the applications of fed-batch culture
and examples of the use of fed batch culture? [16]

4. Write in detail about the methods employed in measuring the following process
variables. [8+8]

(a) Temperature
(b) Pressure.

5. (a) Discuss in detail about ON-Line Sensor.

(b) Discuss in detail about OFF-Line Analytical Methods [8+8]

6. Explain the guide lines for choosing Host-Vector system. [16]

7. Write Short Notes On: [16]

(a) Monoclonal antibodies.

(b) Immunobiological Regulators.
(c) Virus Vaccines.
(d) Hormones.

8. Discuss in detail about the factor which interact to determine pellet formation and
pellet structure during cultivation of mycelial organism. [16]

⋆⋆⋆⋆⋆

1 of 1
Code No: RR322304 Set No. 3
III B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007
BIOPROCESS ENGINEERING-II
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Explain the basis for classification of Bioreactors and write in brief about them
with an example? [16]

2. Explain in detail about the two different design approaches for continuous steril-
ization process ? [16]

3. Write about the different configurations of Air-lift fermentor their design and op-
eration? [16]

4. Explain about the On-line sensors for cell properties? [16]

5. (a) Discuss in detail about ON-Line Sensor.

(b) Discuss in detail about OFF-Line Analytical Methods [8+8]

6. Discuss in detail the control and information sequences in DNA guide the tran-
scription and transaation process which result in gene expression. [16]

7. Discuss in detail about product formation kinetics. [16]

8. Discuss in detail about growth cycle phases for batch cultivation. [16]

⋆⋆⋆⋆⋆

1 of 1
Code No: RR322304 Set No. 4
III B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007
BIOPROCESS ENGINEERING-II
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Define Batch Growth? Explain about the growth patterns and Kinetics in Batch
culture? [16]

2. Explain in detail about two-parameter model for Non ideal reactors? [16]

3. List out the cardinal rules to be followed in design of a fermentor and its construc-
tion materials for successful operation? [16]

4. Explain the methods involved in measurement of process variables such as pH and

dissolved oxygen? [16]

5. (a) Discuss in detail about ON-Line Sensor.

(b) Discuss in detail about OFF-Line Analytical Methods [8+8]

6. Discuss in detail about the overview of information flow in the cell. [16]

7. What are the regulatory constraints on genetic process? [16]

8. Discuss in detail about growth cycle phases for batch cultivation. [16]

⋆⋆⋆⋆⋆

1 of 1