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256
MICROBIOLOGY
B.Sc. (GENERAL) FIRST YEAR (SEMESTER SYSTEM ) EXAMINATION, 2014-15
Note :
1. A student who has passed the + 2 examination under 10+2+3 system of education of a
recognized University/Board/Council or any other examination recognized by the Panjab
University as equivalent thereto shall be eligible to offer the subject of Microbiology at
the B.Sc. level, if he/she has passed the +2 examination with Physics, Chemistry,
Mathematics, Biology as his/her subjects.
2.
Only such colleges which have all necessary infrastructure or equipment and staff shall
admit students to the subject of Microbiology. The infrastructure must be approved by the
University as per usual practice.
Semester-I
Scheme of Examination
Theory
Duration
Marks
6 hrs.
75 (33+33+9*)
MIC 101
: Fundamentals of Microbiology-I
3 hrs.
MIC 102
: Microbial PhysiologyMetabolism-I
3 hrs.
6 hrs.
25 (20+5*)
Practical
One Practical pertaining to the entire syllabus included in Theory
Papers MIC 101 and MIC 102
Semester-II
Theory
6 hrs.
75 (33+33+9*)
MIC 201
: Fundamentals of Microbiology-II
3 hrs.
MIC 202
: Microbial PhysiologyMetabolism-II
3 hrs.
Practical
One Practical pertaining to the entire syllabus included in Theory
Papers MIC 201 and MIC 202
TOTAL MARKS : 200
Note : * Denotes marks for the Internal Assessment.
6 hrs.
25 (20+5*)
257
MICROBIOLOGY
SEMESTER-I
OUTLINES OF TESTS AND SYLLABI
MIC 101 : FUNDAMENTALS OF MICROBIOLOGY-I
Note : The question paper will consist of four sections (A-D). There will be nine questions, out of which
five questions have to be attempted. Question I will span the complete syllabus and will be
compulsory. Rest of the eight questions will be from different sections of the syllabus. There will
be four questions from each of the two sections and two is to be attempted from each
section. Each question should be sub-divided into 2-4 sub parts.
Objectives :
To provide basic knowledge about the fundamental concepts of Microbiology including history of
Microbiology, Microscopic examination of microbes and providing information in frontier areas of genetic
engineering, environmental science and agriculture.
SECTION-A
1.
2.
3.
1.
2.
3.
1.
2.
1.
Microorganism Association with Vascular Plants : Rhizosphere and Rhizoplane microorganisms and
Mycorrhizae.
2.
3.
Biopesticides.
258
Recommended Books :
1.
Fox, S.W., Dose, K. (1994), Molecular Evolution and the Origin of Life, University of Chicago Press.
2.
Stanier, R.Y., Doudoroff, M., Adelberg, E.A. (1999), General Microbiology, Mc Millan Press, London.
3.
Pelczar, M.J., Sun Chan E.C., Krieg, N.R. (1986), Microbiology, Tata Mc Graw Hill Publication, New
Delhi.
4.
5.
6.
7.
8.
9.
Prescott, Harley, and Kleins Microbiology by Joanne M. Willey, Linda M. Sherwood and Christopher J.
Woolverton, Mc Graw Hill (2008).
Microbial Nutrition : Requirements for Growth. Physical requirement (temperature, pH, osmotic
pressure), chemical requirements (C, N, S, P, O).
2.
Culture Media : Chemically defined media, complex media, anaerobic growth media, selective &
differential media, and enrichment culture. Cultivation of Aerobes and Anaerobes.
SECTION-B
1.
2.
3.
Microbial Metabolism :
1.
2.
3.
4.
5.
6.
Glyoxylate cycle.
259
SECTION-D
Bacterial Genetics:
1.
Conjugation.
2.
Transformation.
3.
4.
1.
Stanier R.Y., Doudoroff M., Adelberg, E.A. (1999), General Microbiology, McMillan Press,
London.
2.
Pelczar, M.J., Sun Chan, E.C., Krieg, N.R. (1986), Microbiology, Tata Mc Graw Hill Publication, New
Delhi.
3.
4.
5.
Dubey, R.C., Maheshwari, D.K., (2005), A Text Book of Microbiology, S. Chand and Company.
Microbiology
An
Introduction,
PRACTICALS
1.
Preparation of culture media, spread plates, pour plates, selective media, differential media.
2.
Separation of pure cultures and study the effect of selective nutrients on prokaryotes.
3.
4.
Selective media for Soil microflora and use of growth factors, Study of Rhizosphere interactions,
Quantitative measurements of Soil nutrients and Rhizosphere microflora and preparation of starter
cultures of Rhizobia, Azotobacter.
5.
6.
7.
8.
260
MICROBIOLOGY
SEMESTER-II
MIC 201 : FUNDAMENTALS OF MICROBIOLOGY-II
Note : The question paper will consist of four sections (A-D). There will be nine questions, out of which
five questions have to be attempted. Question I will span the complete syllabus and will be
compulsory. Rest of the eight questions will be from different sections of the syllabus. There will
be four questions from each of the two sections and two is to be attempted from each
section. Each question should be sub-divided into 2-4 sub parts.
Objectives :
To provide basic knowledge about the fundamental concepts of Microbiology including history of
Microbiology, Microscopic examination of microbes and providing information in frontier areas of genetic
engineering, environmental science and agriculture.
SECTION-A
1.
Microscopic examination of micro-organism, bright field microscopy, dark field microscopy, phase
Contrast microscopy, electron microscopy.
2.
1.
2.
3.
Production of pharmaceuticals.
Insect pest control.
Use of Genetically Engineered Microorganisms (GEMs) for control of pollution.
SECTION-D
1.
Biogeochemical CyclingCarbon cycle, Nitrogen cycle, Phosphorus and Sulphur cycle with role of
microorganisms.
2.
261
Recommended Books :
1.
Fox, S.W., Dose, K. (1994), Molecular Evolution and the Origin of Life, University of Chicago Press.
2.
Stanier, R.Y., Doudoroff, M., Adelberg, E.A. (1999), General Microbiology, Mc Millan Press, London.
3.
Pelczar, M.J., Sun Chan E.C., Krieg, N.R. (1986), Microbiology, Tata Mc Graw Hill Publication, New
Delhi.
4.
5.
6.
7.
8.
9.
Prescott, Harley, and Kleins Microbiology by Joanne M. Willey, Linda M. Sherwood and Christopher J.
Woolverton, Mc Graw Hill (2008).
Microbial Growth : Growth in population, bacterial growth curve, mathematical nature and
expression.
Factors affecting growth in microorganisms.
Methods for measurement of growth in microorganisms.
SECTION-B
1.
2.
3.
SECTION-C
Microbial Metabolism :
1. Catabolism of lipids and proteins.
2. Beta oxidation.
3. Electron transport chain.
4. Biochemical mechanisms of generation of ATP.
262
SECTION-D
Microbial Utilization of Energy & Biosynthesis :
1. Transport of nutrient by bacteria.
2. Synthesis of Amino Acids : Glutamate, lysine, glutamine, serine, arginine family.
3. Structures and biosynthesis of cell wall peptidoglycan.
4. Biosynthesis of Carbohydrates (gluconeogenesis) & Phospholipids.
Replication of DNA molecules, Transcription & Translation (process of protein synthesis).
Recommended Books :
1.
Stanier R.Y., Doudoroff M., Adelberg, E.A. (1999), General Microbiology, McMillan Press, London.
2.
Pelczar, M.J., Sun Chan, E.C., Krieg, N.R. (1986), Microbiology, Tata Mc Graw Hill Publication, New
Delhi.
3.
4.
5.
Dubey, R.C., Maheshwari, D.K., (2005), A Text Book of Microbiology, S. Chand and Company.
Microbiology
An
Introduction,
PRACTICALS
1.
Use of microscope in examination of unstained bacteria, fungi, algae, parasites and stained cell
preparations including simple staining, Grams staining, acid fast staining, capsule staining, spore
staining using prokaryotic and eukaryotic cells, hanging drop preparation.
2.
3.
Relation of free oxygen to microbial growth, monitoring of dissolved oxygen in various effluents.
4.
5.
6.
Determination of Water Activity of various substrates and assay of surface active agents.
7.
8.