Professional Documents
Culture Documents
I HC DC H NI
L HU TN
H NI - 2014
B Y T
I HC DC H NI
L HU TN
H NI-2014
MC LC
T VN .............................................................................................................1
CHNG 1. TNG QUAN .......................................................................................2
1.1. TNG QUAN V H GNG (ZINGIBERACEAE) .....................................2
1.1.1. V tr phn loi h GNG (ZINGIBERACEAE) ........................................2
1.1.2. c im thc vt h Gng (ZINGIBERACEAE) .....................................2
1.1.3. Phn loi thc vt h Gng .......................................................................3
1.2. TNG QUAN V CHI HEDYCHIUM ............................................................4
1.2.1. c im thc vt v phn b chi Hedychium ...........................................4
1.2.1.1. c im thc vt chi Hedychium ..........................................................4
1.2.1.2. Phn loi thc vt chi Hedychium ...........................................................4
1.2.1.3. Nhng tc dng c ch ca chi Hedychium .............................................4
1.2.2. c im mt s loi thuc chi Hedychium c Vit Nam ......................5
1.2.2.1. Hedychium coronarium Koenig ..............................................................5
1.2.2.2. Hedychium bousigonianum Pierre .......................................................10
1.2.2.3. Hedychium coccineum Hamilt ..............................................................11
1.2.2.4. Hedychium ellipticum (Smith) Kuntze ..................................................11
1.2.2.5. Hedychium gardnerianum Roscoe ........................................................12
1.2.2.6. Hedychium forresti Diels.......................................................................12
1.2.2.7. Hedychium villosum Wall .....................................................................12
CHNG 2. I TNG V PHNG PHP NGHIN CU ........................14
2.1. NGUYN LIU, PHNG TIN NGHIN CU ......................................14
2.1.1. Nguyn liu nghin cu ...........................................................................14
2.1.2. Phng tin nghin cu............................................................................14
2.1.2.1. Ha cht v dng c ..............................................................................14
2.1.2.2. Thit b dng trong nghin cu .............................................................14
2.2. PHNG PHP NGHIN CU ..................................................................15
2.2.1. Nghin cu thc vt bng phng php nghin cu hin vi ...................15
2.2.2. Nghin cu bng phng php ha hc ...................................................15
LI CM N
Trong thi gian qua, em nhn c rt nhiu s gip , ng vin
ca thy c, gia nh v bn b. Nhng iu ht sc qu bu gip em n
lc hon thnh kha lun ny.
Vi lng knh trng v bit n su sc, em xin gi li cm n chn
thnh ti PGS.TS. Nguyn Vit Thn, ging vin b mn Dc liu trng
i hc Dc H Ni, ngi thy tn tnh hng dn, ch bo em trong
sut qu trnh thc hin kha lun.
Em xin chn thnh cm n cc thy c gio, cc anh ch k thut vin ti
b mn Dc liu gip v to iu kin thun li cho em thc hin
kha lun ti b mn.
Em xin trn trng cm n ban Gim hiu, phng o to, cc thy c
trng i hc Dc h Ni truyn cho em kin thc cng nhit huyt ca
cc thy c trong sut nhng nm hc tp ti trng.
V cui cng, em xin chn thnh cm n gia nh, bn b, ngi thn
bn ng vin, gip em thc hin kha lun.
DANH MC CC K T VIT TT
K t vit tt
Tn y
SKLM
Sc k lp mng
Rf
H s lu
UV
Ultraviolet
TT
Thuc th
GC-MS
Var.
Variate
IC50
DANH MC CC BNG
Bng 3.1
Trang 31
Bng 3.2
Trang 38
Bng 3.3
Trang 39
Ngi tin
DANH MC CC HNH
Hnh 1.1
Trang 7
Hnh 2.1
Trang 16
Hnh 3.1
Trang 17
Hnh 3.2
Trang 20
Hnh 3.3
Trang 20
Hnh 3.4
Mt s c im bt l Ngi tin
Trang 22
Hnh 3.5
Trang 22
Trang 33
(5,5:4,5:0,5)
Hnh 3.7
Trang 33
Trang 34
A.formic (5,5:4,5:0,5)
Kt qu lng gi sc k dch chit MeOH di n
Hnh 3.9
Trang 35
(5,5:4,5:0,5)
Kt qu lng gi sc k dch chit MeOH sau khi
Hnh 3.10 phun TT Vanilin/H2SO4 c h Toluen-EtOAc-
Trang 35
A.formic (5,5:4,5:0,5)
Hnh 3.11
Trang 36
Hnh 3.12
Trang 37
Trang 37
T VN
Vit Nam nm trong vng kh hu nhit i m gi ma nn c thin
nhin u i cho h thc vt phong ph v a dng, trong c bit phi k n
ti nguyn cy thuc.
Theo kt qu iu tra ca Vin Dc liu nm 2006, Vit Nam c 3948 loi
thc vt bc cao, bc thp v nm ln c dng lm thuc. Tuy nhin, phn ln
cc cy thuc c s dng theo kinh nghim dn gian hoc theo Y hc c truyn
m cha c nghin cu mt cch chuyn su v y .
Ngi tin l loi cy bi thn r thng mc ven b hoc c ngi dn
trng lm cnh, lm ba ngi trong tn ngng mt s dn tc thiu s. Thn r c
tc dng cha au d dy, y bng trng hi, cm mo, phong thp nhc mi,
rn cnv cha mt s cht c hot tnh chng ung th ang c th gii quan
tm nghin cu.
Mc d c nhiu tc dng nh vy nhng cc nghin cu hin nay v cy
Ngi tin cn t v cha thc s y . V vy, ti thc hin kha lun NGHIN
CU C IM THC VT, THNH PHN HA HC CA CY NGI
TIN (Hedychium coronarium Koenig), H GNG (ZINGIBERACEAE)
TRNG TRT TI HUYN T LIM, H NI l 1 phn ca ti cp
trng NGHIN CU BO TN V PHT TRIN MT S NGUN GEN
CY THUC QU HIM C TIM NNG VIT NAM nhm mc tiu
gp phn xy dng c s d liu s dng cy mt cch c hiu qu.
thc hin c mc tiu trn, ti c tin hnh vi cc ni dung sau:
1. Nghin cu cc c im thc vt ca mu cy: m t c im hnh thi,
c im vi phu, c im bt l, thn r Ngi tin.
2. Nghin cu thnh phn ha hc ca b phn dng: nh tnh cc nhm cht
bng phn ng ha hc, sc k lp mng.
3. Nghin cu v tinh du: xc nh thnh phn, hm lng tinh du, d tm h
sc k lp mng tinh du ca thn r Ngi tin.
cc chi:
Chi Hedychium
Chi Elettariopsis
Chi Alpinia
Chi Geostachys
Chi Cenolophon
Chi amomum
Chi Catimbium
Chi Curcuma
Chi Languas
Chi Zingiber
Chi Achasma
Chi Stahlianthus
Chi Nicolaia
Chi Kaemfperia
Chi Elettaria
Chi Siliquamomum
Chi Boesenbergia
nhiu nghin cu cho thy tc dng dit khun ca tinh du thn r Ngi tin trn
nhiu loi vi khun nh: Trichoderma, Candida albicans, Pseudomonas
aeruginosa, Bacillus subtilis [20], [31].
Tinh du hoa Ngi tin rt thm, cha ti 175 thnh phn, trong c
linalol, methyl-benzoate, cis-jasmon, methyl-jasmon, methyl epi-jasmonat, indol,
nhiu cht nitril v oxim. Cc cht: trans-ocimen, 2- v 3-methylbutanal oxim,
2-exohydroxy-1,8-cineol, methyl epi-jatmoat, cis-jasmen lacton to ra mi hoa Ngi
tin [5]. Tinh du l c eucalyptol (Trung dc t hi, 1993) [22].
Cu trc ha hc mt s diterpen c hot tnh sinh hc nh sau:
Coronarin A (C20H28O2)
Isoconarin D (C20H30O3)
Coronarin B (C20H30O4)
Coronarin E (C20H28O)
Coronarin D (C20H30O3)
Villosin C (C20H24O6)
Tc dng dc l
-
Tc dng li niu v chng tng huyt p: Cao Ngi tin chit bng cn 50%
t l nhit thp, sau lm bay hi cn, c tc dng li niu v chng
tng huyt p chut cng trng bnh thng v chut cng trng c gy
tng huyt p [5], [20].
Tc dng h st, chng vim, khng khun: Do tinh du l Ngi tin c cha
cht Eucalyptol. Ngoi ra cn lm gin ph qun v gim au [5], [20].
Cha st: Thn r Ngi tin, hnh, tha l (liu lng bng nhau) dng ti,
gi nt, p [5], [29], [30].
Cha au bng lnh, y trng, tiu ha km: Thn r kh hoc qu (612g) sc ly nc hoc tn bt ung [13].
10
11
12
13
mu nu nht, di 2-2,5cm, rng 1cm, khng lp ln nhau. Cnh hoa ph ngn hn,
hoa trng, ng i 2cm. Cnh hoa v tiu nhy lp di 3cm, mi hp, di 2,5cm, x
2 thy su. Vi nhy di 4,5cm, bao phn rt ngn, ch nh di 5cm. Non so c
lng. Ma hoa vo thng 2,3 hng nm [9], [34]. Phn b Bc b [9], [34].
Thnh phn ha hc: Trong thn r cha 14,15,16-trinorlabda-8 (17), 11-(E) dien13-carboxylic acid, 13, 14, 15, 16-tetranorlabda-8 (17)-en-12-carboxylic acid, (E)15,16-dinorlabda-8 (17), 11-dien-13-on, villosin, coronarin E v -sitosterol [33].
14
15
16
Hnh 2.1: Cu to b
dng c ct tinh du ci
tin [2]
A. Bnh cha dc liu
B. B phn treo ng
hng tinh du
C. ng sinh hn
a: hm m dc liu ti
mti: Khi lng thn r ti
mkh: Khi lng thn r kh tuyt i
17
1.nh cy
2.Cm l bc
3.Qu
4.Ht
5.Thn r
18
19
Vng m cng m ly libe (6). B libe-g gia, hng cui t trn xung ln nht
v nh dn v 2 pha. Xen k cc b libe-g hng th 3 l cc m khuyt (5). Ri
rc trong m mm c cc t bo tit tinh du (8).
Phn phin l: Biu b trn v di gm 1 lp t bo hnh ch nht, nh, xp
u n (7). C 2 mt u c l kh. Gia 2 biu b trn, di l m mm gm cc
t bo hnh trn hoc a gic khng u nhau (2). Ri rc trong m mm c cc b
libe-g, g trn, libe di, vng m cng di libe. Ngoi ra cn c cc t bo
tit tinh du (8).
- c im vi phu ca thn r Ngi tin:
Quan st di knh hin vi (Hnh 3.3), ta thy cc c im sau:
Vi phu ct ngang thn r Ngi tin thng c hnh trn. Ngoi cng l lp
bn gm 10-12 lp t bo. 6 lp t bo bn ngoi l cc t bo hnh a gic, 6 lp
bn trong l cc t bo hnh ch nht xp thnh cc dy xuyn tm (1). St lp bn
l m mm v, gm cc t bo hnh trn hoc a gic, thnh t bo mng (2). Ri
rc trong m mm v c cc t bo tit tinh du (3). Pha trong xut hin nhiu m
khuyt (4). Tip theo l ni b gm 1 lp t bo hnh ch nht xp u n (5). St
lp ni b c nhiu b libe-g nm trong m mm rut (6, 7). M mm rut cu to
t cc t bo hnh trn hoc a gic (8), to hn t bo m mm v, xen ln c cc t
bo tit tinh du, cc mch (9).
20
5.M khuyt
2.M mm
6.Vng m cng
3.G
7.Biu b phin l
4.Libe
6.Libe
2.M mm v
7.G
3.T bo tit
8.M mm rut
4.M khuyt
9.Mch
5.Ni b
21
22
2. Biu b mang l kh
6. B si
3. Mnh phin l
2. Mnh bn
3. m tinh bt
6. Ht tinh bt
23
3.2. Nghin cu v ha hc
3.2.1. Ct tinh du
3.2.1.1. Xc nh m thn r ti
Ct nh thn r Ngi tin ti loi b tp, ra sch, lau kh. Sau cn
chnh xc 1g dc liu ti v cho vo ci s nghin nh. a vo my xc nh
m dc liu.
Kt qu: m thn r NGait tin ti: a= 82,48% .
3.2.1.2. Xc nh hm lng tinh du cc b phn Ngi tin
Phng php ct tinh du: Ct ko bng hi nc.
Dng c: S dng b dng c ct tinh du ci tin, B mn dc liu, i hc
Dc H Ni (Hnh 2.1) [2].
Tin hnh:
- Thi nh thn r ti lm sch tp. Sau nghin nt bng thuyn tn.
- Cn dc liu thn r ti mti = 469,82g.
- Cho dc liu vo bnh ct tinh du, nc va ngp mt dc liu. Lp b
dng c ng quy nh.
- Ct trong vng 4 ting (tc ct 2-3ml/pht). Sau ngng ct, sau 15 pht c
th tch tinh du phn chia vch ca dng c hng tinh du.
- Rt ly tinh du vo l thy tinh c np Silicon.
Kt qu:
Vtinh du = 0,3ml
24
25
b. Phn ng diazo ha
Cho 1ml dch chit vo ng nghim, kim ha bng dung dch NaOH, thm
vi git thuc th diazo mi pha, lc u, un nng trn ni cch thy trong vi
pht thy xut hin mu Phn ng dng tnh.
c. Quan st hunh quang ca cc vt coumarin di nh sng t ngoi khi tc dng
vi dung dch kim.
Nh vi git dch chit ln giy thm. Nh tip vi git dung dch NaOH
5%. Sy nh. Che mt phn din tch dch chit trn giy lc bng ng xu ri chiu
tia t ngoi trong vi pht. B ng xu ra, quan st tip di n t ngoi thy phn
khng b che c hunh quang sng ging phn b che Phn ng m tnh.
d. Vi thng hoa
Cho mt t bt thn r Ngi tin vo np chai kim loi. t ln bp in c
li amian, cho bay ht hi nc trong dc liu. t trn ming np nhm mt
phin knh trn c t t bng thm nc lnh. un nh di np nhm, sau
5 pht ly lam knh ra, ngui, soi di knh hin vi khng thy tinh th hnh kim
Phn ng m tnh.
Kt lun s b: Thn r Ngi tin khng cha coumarin.
3.2.2.3. nh tnh saponin.
a. Quan st hin tng to bt
Cho vo ng nghim ln 0,1g bt thn r Ngi tin, thm 5ml nc. Lc
mnh trong 5 pht. yn v quan st thy hin tng to bt bn vng sau
15pht.
b. Hin tng ph huyt
Cho mt git mu b loi fibrin ln phin knh, nh thm mt git dch
chit nc ca thn r Ngi tin. Quan st hin tng ph huyt trn knh hin vi.
c. Phn ng phn bit Saponin steroid v Saponin triterpenoid
Ly 1g bt thn r Ngi tin cho thm 5ml cn un si cch thy trong 15
pht. Ly 2 ng nghim c bng nhau, cho vo ng th nht 5ml HCl 0.1N (pH=1)
v ng th hai 5ml NaOH 0.1N (pH=13). Cho thm vo mi ng 2-3 git dung dch
26
27
c. ng 3: ly 2ml dch lc, thm 5 git dung dch gelatin 1% khng thy xut hin
ta bng trng Phn ng m tnh.
Kt qu: 2 phn ng a, b dng tnh. Phn ng c m tnh.
Kt lun s b: Thn r Ngi tin khng c tannin.
3.2.2.6. nh tnh anthranoid.
a. Phn ng Borntraeger
nh tnh anthranoid ton phn (dng glycosid v dng t do)
Cho vo ng nghim ln 1g thn r Ngi tin. Thm 5ml dung dch acid
sulfuric 1N. un trc tip trn ngun nhit n si. Lc dch chit cn nng qua
mt lp bng mng vo trong bnh gn dung tch 50ml. Lm ngui dch lc. Thm
5ml chloroform, lc nh, gn b lp nc. Gi lp cloroform lm phn ng.
Ly 1ml dch chit cloroform, thm 1ml dung dch amoniac. Lc nh. Khng
thy lp nc xut hin mu sim Phn ng m tnh.
Kt lun s b: Thn r Ngi tin khng c anthranoid.
3.2.2.7. nh tnh glycosid tim.
Ly 3g thn r Ngi tin, chit soxhlet vi n-hexan 1 gi. B dc liu sy
kh, cho vo bnh cu, un hi lu vi ethanol 40% trong 1 gi. Gn dch chit vo
cc c m, thm khong 3ml ch acetat 30%, khuy u. Lc loi ta, th dch lc
vn cn ta vi ch acetat, cho thm 1ml ch acetat na vo dch chit, khuy v lc
li. Tip tc th n khi dch chit khng cn ta vi ch acetat. Cho ton b dch
lc vo bnh gn v lc k vi hn hp chloroform:ethanol t l 4:1 (3 ln, mi ln
5ml), gn ly lp chloroform vo cc c m kh sch. Chia dch chit vo 6 ng
nghim nh, bc hi dung mi trn ni cch thu cho n kh. Cn cn li lm
cc phn ng nh tnh sau:
a. Phn ng ca khung steroid: Phn ng Liebermann
Cho vo ng nghim c cha cn trn 1ml anhydrid acetic, lc u cho tan
ht cn. Nghing ng 450. Cho t t theo thnh ng 0,5ml acid sulfuric c, trnh
xo trn cht lng trong ng. Nhn thy mt tip xc gia hai lp cht lng xut
28
Phn ng m tnh.
- Phn ng Legal
Cho vo ng nghim c cha cn trn 0,5ml ethanol 90%. Lc u cho tan
ht cn. Nh 1 git thuc th Natri nitroprussiat 0,5% v 2 git dung dch NaOH
10%. Lc u. Phn ng thy xut hin mu cam Phn ng m tnh.
c. Phn ng ca phn ng 2,6 - desoxy: Phn ng Keller - Kiliani
Cho vo ng nghim cha cn trn 0,5ml ethanol 90%. Lc u cho tan ht
cn. Thm vi git dung dch st (III) clorid 5% pha trong acid acetic. Lc u.
Nghing ng 450. Cho t t theo thnh ng 0,5ml acid sulphuric c, trnh xo trn
cht lng trong ng. mt tip xc gia 2 lp cht lng xut hin 1 vng mu tm
. Lc nh, lp cht lng pha trn c mu xanh l Phn ng dng tnh.
Kt qu: Ch c phn ng a v c dng tnh.
Kt lun s b: Thn r Ngi tin khng c glycosid tim.
3.2.2.8. nh tnh acid hu c
Cn khong 3g bt thn r Ngi tin cho vo ng nghim to, thm 10ml nc
ct em un si trc tip 10 pht, ngui ri lc qua giy lc gp np. Cho vo
ng nghim nh khong 2ml dch lc, thm mt t tinh th Na2CO3. Thy c bt kh
thot ra Phn ng dng tnh.
Kt lun s b: Thn r Ngi tin c cha acid hu c.
3.2.2.9. nh tnh ng kh
Cn khong 3g thn r Ngi tin cho vo ng nghim to, thm 1ml nc ct,
un si. i vi cao s dng 1g v ha tan bng nc nng. Lc qua giy lc vo 1
29
ng nghim khc. Thm 1ml dung dch thuc th Felling A v 1ml dung dch
Felling B. un cch thy si vi pht. Thy xut hiu ta gch Phn ng
dng tnh.
Kt lun s b: Thn r Ngi tin cha ng kh.
3.2.2.10. nh tnh acid amin
Cn khong 3g thn r Ngi tin cho vo cc c m, thm 20ml nc ct,
un si vi pht. i vi cao s dng 1g v ha tan bng nc nng. Lc qua giy
lc vo 1 ng nghim khc. Thm vi git thuc th Ninhydrin 3%. un cch thy
si vi pht. Thy xut hiu mu tm Phn ng dng tnh.
Kt lun s b: Thn r Ngi tin c cha acid amin.
3.2.2.11. nh tnh polysaccharid
Chun b dch chit nh i vi phn ng nh tnh acid amin. Chia dch chit vo 3
ng nghim:
ng 1: 4ml dch chit + 5 git thuc th Lugol
ng 2: 4ml nc ct + 5 git thuc th Lugol
ng 3: 4ml dch chit.
Thy mu ng 1 m hn ng 2 v ng 3 Phn ng dng tnh.
Kt lun s b: Thn r Ngi tin cha polysaccharid.
3.2.2.12. nh tnh cht bo
Cn khong 3g bt thn r Ngi tin vo cc c m 100ml, thm 10ml ether
du ha, bc kn, ngm 1 gi. Lc qua giy lc gp np ly dch lc. Nh 1 git
dch chit ln mnh giy trng, sy nh cho bay ht hi dung mi, khng thy vt
m trn giy Phn ng m tnh.
Kt lun s b: Thn r Ngi tin khng cha cht bo.
3.2.2.13. nh tnh caroten
Cho vo ng nghim nh khong 2ml dch chit ether du ha ca thn r
Ngi tin, c cch thy n cn, nh vi git H2SO4 c vo cn. Khng thy xut
hin mu xanh l Phn ng m tnh.
Kt lun s b: Thn r Ngi tin khng cha caroten.
30
31
Flavonoid
2
Glycoside tim
Saponin
Coumarin
Tanin
Anthranoid
Alcaloid
Phn ng nh tnh
Kt qu
Phn ng vi hi NH3
++
Phn ng vi dd FeCl3 5%
Phn ng Cyanidin
++
Phn ng diazo ha
Phn ng Libermann
Phn ng Legal
Phn ng Baljet
Phn ng Keller-Kiliani
++
Phn ng diazo ha
Vi thng hoa
Phn ng vi dd FeCl3 5%
Phn ng vi dd gelatin 1%
Phn ng Borntraeger
Phn ng vi TT Mayer
Phn ng vi TT Dragendorff
Phn ng vi TT Bouchardat
Kt lun
Khng c
Khng c
Khng c
Khng c
Khng c
Acid hu c
Phn ng vi Na2CO3
ng kh
++
10 Acid amin
Phn ng vi TT Ninhydrin 3%
++
11 Cht bo
To vt m trn giy
Khng c
12 Caroten
Phn ng vi H2SO4 c
Khng c
13 Sterol
Phn ng Liebermann
14 Polysaccharid
Phn ng vi TT Lugol
++
15 Iridoid
Khng c
- Phn ng m tnh
32
33
H dung mi
-H 1: Toluen-Ethyl acetat-Acid formic (7:3:0,1)
-H 2: Toluen-Ethyl acetat (7:3)
-H 3: n-Hexan-Ethyl acetat-Acid formic (6:4:0,5)
-H 4: Toluen-Ethyl acetat-Methanol-Acid formic (4:5:0,5:1)
Thuc th hin mu: Vanilin/H2SO4 c
Kt qu: Sau khi tin hnh khai trin sc k dch chit sau khi thy phn trn cc h
dung mi khc nhau th nhn thy h 1 c kh nng tch cht tt nht.
a. Hnh nh sc k sau khi chp bng my CAMAG REPROSTAR 3
A.formic (5,5:4,5:0,5)
EtOAc-A.formic (7:3:0,1)
a.Sc k di n 254nm
a.Sc k di n 254nm
b.Sc k di n 366nm
b.Sc k di n 366nm
34
35
Toluen-EtOAc-
A.formic (5,5:4,5:0,5)
36
37
38
Ct sc k: HP-5MS
+ Chiu di ct: 30cm
+ ng knh ct: 0,25mm
Ct
Nhit (C)
0-2
50
2-27
50-100
27-33,7
100-200
33,7-35,7
200
Nhit ha hi mu
250
39
Bng 3.3: Thnh phn v hm lng cc cht trong tinh du thn r Ngi tin
STT
Tn cht
Thi
gian lu Din tch Pic
(pht)
Hm
lng
(%)
Thujen
8,447
1947606
0,262
Pinen
8,717
45924867
6,184
Camphen
9,372
3069936
0,413
4
5
6
7
8
Sabinen
Pinen
Myrcen
Phellandren
3-Caren
10,616
10,725
11,601
12,213
12,523
7138946
168363778
6523434
9537142
2502720
0,961
22,671
0,878
1,248
0,337
Terpinen
12,900
1968601
0,265
10
o,p-Cymen
13,355
17044444
2,295
11
Limonen
13,577
31659304
4,263
12
1,8-Cineol
13,691
300701284
40,491
13
-Terpinen
15,382
7935689
1,069
14
Terpinolen
17,169
3299610
0,444
15
Linalool
18,018
3645854
0,491
16
Borneol
22,004
9757740
1,314
17
18
4-Terpineol
Terpineol
22,804
23,715
32607466
72369287
4,391
9,745
19
Terpinolen
30,428
5540300
0,746
20
Caryophyllen
31,588
2918656
0,393
21
Caryophyllen oxid
33,543
2851220
0,384
22
Spathulenol
33,948
1284150
0,173
23
4-Isobutylidene-2,2dimethyl-8-oxa bicycle
[3.2.1] oct-6-en-3-on
34,006
1545364
0,208
24
Longifolenaldehyd
34,459
Tng =
2507968
742645366
0,338
100%
40
Nhn xt: Kt qu GC-MS cho thy trong tinh du Thn r Ngi tin c
10 thnh phn chnh l: pinen (6,18%), sabinen (0,961%), pinen (22,671%),
cymen (2,295%), limonen (4,263%), 1,8-cineol (40,491%), borneol (1,314%),
myrcen (0,878%), 4-terpineol (4,391%), terpineol (9,745%). Trong , hm lng
1,8-cineol v -pinen rt cao. Ta i chiu vi sc k sau khi phun thuc hin
mu c 10 vt hin mu r rt, c phn tch bi phn mm VideoScan ( nhy
25pixels, hmin=30, Smin=300) tng ng vi 10 Pic. iu chng t SKLM kt
hp x l bng WinCat v VideoScan l phng php n gin nhng tng i
chnh xc.
41
BN LUN
Cy Ngi tin c ngi dn s dng cha 1 s bnh thng thng nh:
au bng lnh, kh tiu, phong thp, rn cn nc ta cng nh trn th gii,
ngoi nhng cng dng ngi ta cn i su vo nghin cu tc dng chng ung
th ca cc Diterpen phn lp t thn r cho nhiu kt qu kh quan. Cy pht trin
tng i tt khi c trng trt ly hoa lm cnh 1 s a phng. Tuy c
nhiu tc dng tt v tim nng pht trin ln nhng hin nay cy mi ch c
dng theo kinh nghim dn gian m cha pht huy c ht gi tr s dng. V vy,
vic tin hnh nghin cu c im thc vt, thnh phn ha hc ca cy l rt cn
thit, nhng kt qu t c s l c s d liu xy dng tiu chun kim
nghim cho cy Ngi tin khi c s dng trn quy m ln.
V phng php nghin cu: Ngoi nhng phng php thng qui nghin
cu v thc vt, ha hc nh vi hc, nh tnh phn ng ha hc, SKLM th vic kt
hp cc phng php hin i vo nghin cu dc liu ni chung v Ngi tin ni
ring l rt hu ch. Cc phn tch sc k ca phn mm WinCats v Video Scan
va chnh xc li va tin li khi cho ta lun kt qu s vt, Rf, Spic tng vt m
khng phi lm th cng. Phng php GC-MS li gip chng ta nh tnh v nh
lng cc thnh phn d bay hi, trong c tinh du. Vic ny gip chng ta
nghin cu chuyn su hn v c nh gi chnh xc hn.
V c im thc vt, tin hnh nghin cu c im hnh thi cy, c
im vi phu, c im vi hc bt l v thn r Ngi tin. y l ln u tin cc
c im thc vt ca cy c nghin cu 1 cch y , trong khi cc nghin cu
trc ch dng li m t hnh thi cy, phn tch hoa .
V ha hc, tin hnh kim nghim bt thn r ca cy bng cc phn ng
ha hc c trng. Tin hnh d tm h dung mi chy sc k cho dch chit
MeOH, dch chit thy phn, tinh du v kt hp x l bng phn mm chuyn
dng cho kt qu nhanh, chnh xc.
V nghin cu tinh du, tin hnh xc nh thnh phn ha hc, hm
lng tinh du trong thn r Ngi tin bng GC-MS cho kt qu rt chi tit. Trong
42
43
Kin ngh
Ngi tin l cy dc liu ang cn tng i mi, cha c nghin cu 1
cch y v chuyn su. Trn th gii cng nh Vit Nam, ngi ta quan tm
ch yu ti tc dng chng ung th ca cc Diterpen trong thn r. V vy, ngoi
kt qu nghin cu ca ti cng nh nhng nghin cu cng b trc ,
chng ti xut nghin cu thm thnh phn ha hc tinh du hoa, l ca cy
Ngi tin cng nh tc dng sinh hc ca chng v ca tinh du thn r. Nghin
cu a Ngi tin vo trng trt quy m ln khai thc ht tim nng ca cy.
44
14. Nguyn Th Kim Thy (2013), Nghin cu thnh phn ha hc v hot tnh
sinh hc cy Ngi tin Hedychium coronarium Koenig, Lun n Tin s ha
hc, Vin ha hc cc hp cht thin nhin.
15. Nguyn Th Thu, v CS (2003), Thnh phn ho hc ca tinh du Ngi tin
(Hedychium coronarium Koenig), Vin Sinh thi & Ti nguyn Sinh vt,
Vin KH& CNVN.
TI LIU TING ANH
16. Ajay Kumar Meena, et al (2010), Comparative Study on Family
ZingiberaceaePlants Used In Ayurvedic Drugs, International Journal of
Pharmaceutical and Clinical Research, 2(2), pp. 58-60.
17. Armen Takhtajan (2009), Flowering Plants, Springer, pp. 707.
18. Beena Joy, et al (2007), Antimicrobial activity andchemical composition of
essential oil from Hedychium coronarium, Phytotherapy Research,Volume
21, Issue 5, pp. 439443.
19. Flora of China, volume 24, pp. 270.
20. Hamidou F. Sakhanokho, et al (2013), Chemical Composition, Antifungal
and Insecticidal Activities of HedychiumEssential Oils, Molecules, 18, pp.
4308-4327.
21. Hans Wohlmuth (2008), Phytochemistry and pharmacology of plants from
the ginger family , Zingiberaceae, Southern Cross University.
22. Jiau-Ching Ho (2011), Antimicrobial, Mosquito Larvicidal and Antioxidant
Properties of the Leaf and Rhizome of Hedychium coronarium, Journal of the
Chinese Chemical Society, 58, pp. 563-567.
23. Kajaria Divya, et al, Study of Antiasthmatic Properties and Chemical
Characterization
of
Indigenous
Ayurvedic
Compounds
(Polyherbal
PH LC
1. PHIU GIM NH TN KHOA HC
2. PH GC-MS TINH DU THN R NGI TIN