Nghiên cứu nhân nhanh invitro cây chuối tiêu hồng

B GIO DC V O TO
TRNG I HC NNG NGHIP H NI

------ ------
V VN I
NGHIN CU NHN NHANH INVITRO

CY CHUI TIU HNG
LUN VN THC S
H NI 2013
B GIO DC V O TO
TRNG I HC NNG NGHIP H NI
------ ------
V VN I
NGHIN CU NHN NHANH INVITRO

CY CHUI TIU HNG
CHUYN NGNH
: CNG NGH SINH HC
M S
: 60.42.02.01
NGI HNG DN KHOA HC

PGS.TS PHAN HU TN
H NI 2013
LI CAM OAN
Ti xin cam oan rng s liu v kt qu nghin cu trong lun vn ny l
trung thc v khng trng lp vi cc ti khc.
Ti cng xin cam oan rng cc thng tin trch dn trong lun vn u c
ghi r ngun gc.
Hc vin
V Vn i
Trng i hc Nng nghip H Ni Lun vn thc s khoa hc Nng nghip
ii
LI CM N
Trong qu trnh thc hin lun vn, ti nhn c s quan tm,
gip ca nhiu c nhn v c quan n v. Nay lun vn hon thnh,
ti xin by t lng bit n chn thnh, su sc ti:
PGS. TS. Phan Hu Tn, ngi tn tnh hng dn v to mi iu
kin, gip ti nghin cu v thc hin ti.
Cc thy gio, c gio thuc Khoa Cng ngh sinh hc, trng i
hc Nng nghip H Ni nhit tnh ging dy v to mi iu kin cho ti
hon thnh kha hc.
Cc cn b, k thut vin phng ng dng tin b KHCN S Khoa hc
v Cng ngh Bc Ninh nhit tnh hng dn, gip v to mi iu kin
cho ti thc hin lun vn. Phng Cng ngh t bo thc vt - Vin Di truyn
Nng nghip Vit Nam cung cp mu vt nghin cu.
Cui cng, ti xin chn thnh cm n nhng ngi thn trong gia nh
v bn b gip v ng vin ti trong sut thi gian hc tp.
Bc Ninh, thng 9 nm 2013
Hc vin
V Vn i
iii
MC LC
Li cam oan
ii
Li cm n
iii
Mc lc
iv
Danh mc cc ch vit tt
vii
Danh mc bng
viii
Danh mc hnh
ix
M U
CHNG 1 TNG QUAN TI LIU
1.1
VI NT V PHN LOI V C IM CA CHUI
1.1.1
c im thc vt hc ca h chui
1.1.2
c im thc vt hc ca chi chui Tiu.
1.1.3
c dim thc vt hc ca chui Tiu Hng.
1.2
NG DNG CA K THUT NUI CY M T BO

THC VT TRONG CNG TC NHN GING CY
TRNG
1.2.1
Cc hng nghin cu ng dng
1.2.2
u th ca nhn ging in vitro
1.2.3
Cc phng thc nhn ging in vitro
1.2.4
Quy trnh nhn ging in vitro
1.3
MT S NGHIN CU NHN GING CHUI BNG K

THUT NUI CY IN VITRO
11
1.3.1
Cc nghin cu trong nc
11
1.3.2
Cc nghin cu ca nc ngoi
13
CHNG 2 VT LIU V PHNG PHP NGHIN CU
14
2.1
14
VT LIU, A IM V THI GIAN NGHIN CU
iv
2.1.1
Vt liu thc vt
14
2.1.2
Ha cht v thit b
14
2.1.2
a im v thi gian nghin cu.
14
2.2
NI DUNG NGHIN CU
15
2.3
PHNG PHP NGHIN CU
15
2.3.1
Phng php b tr th nghim
15
2.3.2
Phng php pha mi trng v nui cy
15
2.3.2
Phng php ra cy
16
2.3.3
Phng php b tr th nghim
17
2.3.4
Phng php thu thp s liu v x l s liu
23
2.3.5
Phng php tnh ton kt qu
24
CHNG 3 KT QU V THO LUN

3.1
So snh nh hng ca mi trng nui cy MS, B5 v

Phytamax ti s pht sinh chi v to cm chi chui Tiu Hng.
3.2
32
nh hng ca cht kch thch sinh trng IAA n s pht sinh

r ca chui Tiu Hng.
3.3.3
32
nh hng ca cht kch thch sinh trng -NAA n s pht

sinh r ca chui Tiu Hng.
3.3.2
30
nh hng ca cc cht kch thch sinh trng thuc nhm auxin

ti s pht sinh r chui Tiu Hng.
3.3.1
27
nh hng ca kinetin ln s pht sinh chi v to cm chi

chui Tiu Hng.
3.3
27
nh hng ca BAP ti s pht sinh chi v to cm chi chui Tiu

Hng.
3.2.2
25
nh hng ca cc cht kch thch sinh trng thuc nhm

cytokinin ti s pht sinh chi v to cm chi chui Tiu Hng.
3.2.1
25
33
nh hng ca cht kch thch sinh trng IBA n s hnh

thnh r ca chui Tiu Hng.
34
3.4
nh hng ca t hp cc cht kch thch sinh trng thuc

nhm cytokinin v auxin n s sinh trng ca chui Tiu
Hng.
3.4.1
nh hng ca t hp cht kch thch sinh trng BAP v NAA n s sinh trng ca chui Tiu Hng.
3.4.2
3.5
39
nh hng ca t hp cht kch thch sinh trng kinetin v IAA

n s sinh trng ca chui Tiu Hng.
3.4.6
38
nh hng ca t hp cht kch thch sinh trng kinetin v NAA n s sinh trng ca chui Tiu Hng.
3.4.5
37
nh hng ca t hp cht kch thch sinh trng BAP v IBA

3.4.4
36
nh hng ca t hp cht kch thch sinh trng BAP v IAA

3.4.3
36
41
nh hng ca t hp cht kch thch sinh trng kinetin v IBA

42
nh hng ca gi th n s sinh trng ca chui Tiu Hng.
44
KT LUN V KIN NGH
46
KT LUN
46
KIN NGH
46
TI LIU THAM KHO
48
PH LC
51
vi
DANH MC CC CH VIT TT
-NAA
Axit -naphthaleneacetic
ABA
Axit abscisic
BAP
Benzylamino purine
IAA
Axit indol axetic
IBA
Axit indol butyric
KC
Knudson C
MS
Murashige and Skoog
RE
Robert Ernst
VW
Vacin and Went
vii
DANH MC BNG
STT
3.1
Tn bng
Trang
So snh nh hng ca mi trng MS, B5 v Phytamax ti s

pht sinh chi v to cm chi chui Tiu Hng.
3.2
nh hng ca BAP ti s pht sinh chi v to cm chi chui

Tiu Hng.
3.3
25
29
nh hng ca kinetin ti s pht sinh chi v to cm chi chui

Tiu Hng.
31
3.4
nh hng ca -NAA ti s pht sinh r chui Tiu Hng
32
3.5
nh hng ca IAA ti s pht sinh r chui Tiu Hng
33
3.6
nh hng ca IBA ti s pht sinh r chui Tiu Hng.
35
3.7
nh hng ca t hp cht kch thch sinh trng BAP v NAA ti s sinh trng ca chui Tiu Hng.
3.8
nh hng ca t hp cht kch thch sinh trng BAP v IAA

ti s sinh trng ca chui Tiu Hng.
3.9
3.13
40
nh hng ca t hp cht kch thch sinh trng kinetin v IAA

3.12
39
nh hng ca t hp cht kch thch sinh trng kinetin v NAA ti s sinh trng ca chui Tiu Hng.
3.11
38
nh hng ca t hp cht kch thch sinh trng BAP v IBA

3.10
37
41
nh hng ca t hp cht kch thch sinh trng kinetin v IBA

43
Kt qu ra cy
44
viii
DANH MC HNH
STT
3.1
Tn hnh
Trang
So snh nh hng ca mi trng MS, Phytamax v B5 ti s

3.2
nh hng ca kinetin ti s pht sinh chi v to cm chi

chui Tiu Hng
3.3
31
nh hng ca cc cht kch thch sinh trng thuc nhm auxin

ti s pht sinh r ca chui Tiu Hng.
3.4
27
35
Hnh nh sinh trng ca chui Tiu Hng trn gi th t + ct

+ tru hun (1:1:1)
45
ix
M U
1. T VN
Cng ngh nui cy m - t bo thc vt tri qua hn mt trm nm
hnh thnh v pht trin, em li gi tr to ln cho loi ngi. Hin nay, hu
ht cc c s nghin cu ging cy trng trn th gii u p dng cng ngh
ny vi cc mc ch khc nhau.
Vit Nam, k thut nui cy m v t bo thc vt c bt u
nghin cu v ng dng t gia nhng nm 70 ca th k XX. Nhng kt qu
bc u trong nghin cu v ng dng t kt qu kh quan i vi mt
s i tng cy trng nh khoai ty, ma, la, c bit l chui.
Vit Nam nm trong khu vc nhit i gi ma, nng m ma nhiu, l
iu kin l tng cho s sinh trng v pht trin ca chui. Chui nh mt
loi cy quan trng trong chuyn dch c cu cy trng trong nng nghip ven
. Trong th gii cc loi chui, chui Tiu Hng c a chung hn c.
Chui Tiu Hng c mu sc p, chn ca qu ng u. Ngoi gi tr dinh
dng, chui cn c ngha ln trong nn kinh t quc dn. Ging chui ny t
lu c con ngi thun ho, su tm to ra ging nh mun phc v
nhu cu ca con ngi. Cng vi s pht trin ca khoa hc cng ngh, chui
Tiu Hng c nhn ging bng cng ngh nui cy m hin i vi h s
nhn ging cao, sch bnh, cy cho thu hoch ng lot. Hin nay quy trnh
sn xut cy ging bng nui cy m t bo thc vt c hon thin bi
cc Vin nghin cu nh ( Vin Rau qu, Vin Di truyn Nng nghip,
Trng i hc Nng nghip H Ni...). Hng nm cc Vin cung cp rt
nhiu cy ging n tay ngi sn xut. Tuy nhin cy ging sn xut bng
nui cy m thng t v ch ng cung cp ngun cy ging ti ch,
theo yu cu thi v v sn xut hng ha tp trung th vic sn xut cy ging
in vitro trc tip ti cc tnh l la chn ti u c v kinh t cng nh tng

bc nng cao trnh chuyn mn cho i ng k thut vin ti cc tnh.
Hin nay ti Bc Ninh sau khi thc hin ch trng dng sn xut gch
th cng mt bng bi g gh, phn ln din tch hin vn b hoang ha tng
din tch t bi sn xut nng nghip l 2400ha. Do vy vic a vo trng
cy chui Tiu Hng c nhn ging bng cng ngh nui cy m t bo
gp phn quan trng vo vic khai thc tim nng v t ai, lao ng tng
bc pht trin mt nn sn xut nng nghip hng ha c gi tr kinh t cao
trn c s p dng nhng tin b khoa hc cng ngh tin tin. Vi mc tiu
th Trung tm Thng tin v ng Dng tin b khoa hc cng ngh c
nhim v a cy chui nhn nhanh in vitro vo ti tnh nhm h gi thnh
cy ging v ch ng cung cp cy ging p ng nhu cu sn xut l cn thit.
Xut pht t yu cu chng ti tin hnh nghin cu ti:
Nghin cu nhn nhanh invitro cy chui Tiu Hng (M.acuminata)
trong iu kin thc t phng th nghim ca a phng nhm nhn
nhanh ging chui Tiu Hng sch bnh, gp phn nng cao hiu qu cho
ngi trng chui v thc y vic p dng tin b khoa hc cng ngh
vo sn xut nng nghip tnh Bc Ninh.
2. MC CH, YU CU CA TI
2.1. Mc ch
- Tm c mi trng ti u trong nhn ging in vitro chui Tiu Hng.
- Tm c gi th thch hp nht a cy chui Tiu Hng t trong
ng nghim ra mi trng t nhin.
2.2. Yu cu
- Xc nh c mi trng nui cy tt nht.
- Xc nh c mi trng thch hp cho vic nhn nhanh cho h
s nhn cao v cht lng tt.
- Xc nh c mi trng thch hp cho vic to r cho chi v mi
trng thch hp cho s sinh trng ca chui Tiu Hng.

- Xc nh c gi th thch hp cho cy con ngoi vn m.
3. NGHA KHOA HC V THC TIN.
3.1. ngha khoa hc.
- Cc kt qu thu c s lm c s cho vic nghin cu v ging dy
i vi cy chui Tiu Hng.
3.2. ngha thc tin.
- L c s cho vic xut c quy trnh nhn ging in vitro v thch
ng cho cy chui Tiu Hng cho phng th nghim nui cy m ti Bc Ninh.
- Gp phn bo tn, thc y sn xut cy chui Tiu Hng nh mt
ngh trng chui mang li gi tr kinh t ti Bc Ninh v cc vng ln cn.
CHNG 1. TNG QUAN TI LIU

1.1. VI NT V PHN LOI V C IM CA CHUI
1.1.1. c im thc vt hc ca h chui
Ngnh
: Ngc Lan (Mangolophya)
Lp
: Hnh (Liliopsida)
Phn lp
: Hnh (Lilidae)
: Gng (Zingibereles)
: Chui (Musacea)
H chui gm 2 chi vi 70 loi, trong : chi Ensete gm 10 loi, phn b

ch yu vng Chu Phi; chi Musa gm 60 loi phn b cc vng nhit i [5].
Cy chui c ngun gc t ng Nam , sau c di thc sang
Chu c ri ti cc nc Trung v Nam M. Mt s tc gi cn cho rng t
ng Nam cy chui c chuyn qua Madagasca vo lc a Chu Phi,
sau ti cc o Canari v Santodomigo (Champion,1976) [4].
H chui gm 2 chi vi 70 loi, trong : chi Ensete gm 10 loi, phn b
ch yu vng Chu Phi; chi Musa gm 60 loi phn b cc vng nhit i [5].
Cy chui c ngun gc t ng Nam , sau c di thc sang
Chu c ri ti cc nc Trung v Nam M. Mt s tc gi cn cho rng t
ng Nam cy chui c chuyn qua Madagasca vo lc a Chu Phi,
sau ti cc o Canari v Santodomigo (Champion,1976) [4].
1.1.1.1. R chui
R chm, c 2 loi l r ngang v r thng .
R ngang: mc xung quanh c chui v phn b lp t mt t 0 30cm, phn nhiu tp trung su 0,15cm, b ngang rng ti 2 - 3 cm loi
r ny sinh trng khe, phn b rng, l loi r quan trng nht ht
nc v dinh dng nui cy.
R thng: mc pha di c chui, n su tc dng ch yu gi cy

ng vng. R chui cha nhiu nc, gin, mm, yu, d gy ; sc chu hn,
chu ng u km so vi nhiu loi cy n tri khc [7;22].
1.1.1.2.Thn tht
Thn tht hay cn c gi l c chui, c hnh trn dt v ngn, khi pht
trin y c th rng 30cm. Phn bn ngoi xung quanh c chui c bao
ph bi nhng vt so t b l c dng trn. y mi b l u c mt chi
mm nhng ch cc chi phn gia c l pht trin c, c khuynh hng
mc tri dn ln. Cc so l mc rt gn nhau lm thnh khong cch rt ngn.
C chui sng lu nm, l c quan ch yu d tr cht dinh dng, ng thi l
ni r, l, mm v cung hoa mc ra. Do c chui to mp l c s m bo
cho cy sinh trng nhanh, nng sut cao. Xung quanh c chui c nhiu mm
ng, sau ny s pht trin thnh cy con [22].
1.1.1.3.Thn gi v l
Thn cy chui l thn gi, hnh tr do nhiu b l lng vo nhau lm
thnh. Khi mm chui mi mc ln th bt u mc ra nhng l vy (khng
c thn l) c tc dng bo v mm chui. Tip mc ra loi l di v hp
gi l l kim . V sau mc ra nhng l to bnh thng gi l l tht. n
khi mm hoa phn ha th mc ra mt l cht nh, ngn c tc dng che ch
bung chui.
L chui pht trin mnh nht t thng 5 n thng 8, mi thng mc ra
3 - 4 l, phin l to, dy, mu xanh m v bng. T thng 10 tr i, cch 2 3 tun mi ra 1 l mi, l thng mng, nh, mu xanh nht, sinh trng
chm. n thng 12 - 1 mi thng ch mc c 1 l [22].
1.1.1.4.Hoa chui
Cy chui con sau khi mc (hoc sau khi trng) 8 - 10 thng bt u hnh
thnh mm hoa, sau khong 1 thng bt u tr bung. Hoa chui thuc loi
hoa chm gm 3 loi: hoa ci, hoa lng tnh v hoa c [22].
Hoa ci: tp trung pha gc cung bung, phn ny di nht (50 - 100
cm). Loi hoa ny n ra trc tin, nh ci pht trin, nh c thoi ha. Ch
c hoa ci l pht trin thnh tri c. Do , khi trng, chn lc cy ging
tt, chm bn kp thi hnh thnh nhiu hoa ci l nhn t quan trng bo
m nng sut cao [22] .
Hoa lng tnh: nm phn gia bp chui, loi hoa ny khng nhiu
lm, v sau s rng v khng hnh thnh tri c [22].
Hoa c: nm pha u bp chui, nh ci thoi ha, nh c pht
trin, di bng nh ci. Loi hoa c khng th hnh thnh tri c sau ny s
kh i v rng dn [22].
1.1.2. c im thc vt hc ca chi chui Tiu.
Chui tiu thuc loi cy tho, sng lu nm, thn cy trn, mm,
thng, c b l. Cung hnh trn c khuyt rnh, l to, di. Tri nm trn
bung, c t 6-8 ni, mi ni khong 12 tri. Tri nh, di, mi thm. Khi
chn, v vn mu xanh nhng khi chn mi th mu vng. Qu chui c v
ngt, tnh rt lnh (tnh hn), khng c.
1.1.3. c dim thc vt hc ca chui Tiu Hng.
Ging chui tiu hng thuc nhm ph chui tiu va. Thn gi mu
hng , c nhng mng en nu ln lin tc. L ng, u l un cong, gn
chnh mu trng sng gc c nhiu phn trng, ui gn l t phn.
- Thn gi cao 2,2-2,6 m, ng knh 17,9-18,3 cm.
- Thi gian t trng n thu hoch 10-11 thng.
- Bung qu hnh tr c 9 ni, 168 qu. Khi lng bung 20,4 kg.
- Kch thc qu: di 18,3 cm, ng knh 3,9 cm
(http://kienthuctamnong.gov.vn)
1.2. NG DNG CA K THUT NUI CY M T BO THC

VT TRONG CNG TC NHN GING CY TRNG
1.2.1. Cc hng nghin cu ng dng
Nhn ging in vitro l mt trong nhng ng dng chnh ca cng ngh t
bo thc vt, s dng s pht trin nhn to v nhn cc im sinh trng
hoc cc m phn sinh ca cy. Theo cc cng trnh th nghim ca Amato
(1977), ch c nh sinh trng ca chi mi m bo s n nh v di truyn,
tip n l m phn sinh vi kch thc nh, kt hp vi x l nhit lm
sch bnh l nguyn liu tt cho nhn ging.
K thut nhn nhanh c ng dng nhm phc v cho cc mc ch
chnh nh: duy tr v nhn nhanh cc kiu gen qu him, lm vt liu cho
cng tc chn ging; duy tr v nhn nhanh cc c th u dng cung cp
ht ging cho cc loi cy trng khc nhau nh cy lng thc c c, cy rau,
cy cnh...; nhn nhanh iu kin v trng cch ly ti nhim kt hp vi
vic lm sch bnh virut, rt ngn thi gian a cc cy lai v cc cy t
nhin c c im tt; bo qun tt tp on ging v tnh v cc loi cy
giao phn trong ngn hng gen [2], [19].
1.2.2. u th ca nhn ging in vitro
H s nhn cao, rt ngn thi gian a ging vo sn xut. T mt cy
trong vng 1-2 nm c th to thnh hng triu cy. Nhn c s lng cy
ln trong mt din tch nh, trong 1m2 c th 18000 cy [19], [26].
Lm sch bnh cy trng v cch ly chng vi ngun bnh, v vy m
bo ging sch bnh; thun tin trong vn chuyn v bo qun (cy ging gi
40C trong hng thng vn cho t l sng 95%). ng thi, c th sn xut
quanh nm m khng ph thuc vo ma v, c tim nng cng nghip ho
cao [26].
1.2.3. Cc phng thc nhn ging in vitro

1.2.3.1. Nui cy m phn sinh hoc nh sinh trng
Theo L Trn Bnh (1997), m phn sinh nui cy l mu vt nui cy
c tch t nh sinh trng c kch thc trong vng 0,1mm tnh t chp
ca chp nh sinh trng. Tuy nhin trong thc t vic nui cy mu vt nh
vy rt kh thnh cng, ngi ta ch tin hnh nui cy khi mc ch l lm
sch virut cho cy trng. Nui cy m phn sinh hoc nh sinh trng c
tin hnh ph bin nht cc i tng chui, phong lan, da, ma...
Trong nui cy m phn sinh hoc nh sinh trng cn ch ti
tng quan gia ln chi, t l sng v mc n nh v mt di truyn
ca chi, v thng thng nu ln ca chi tng th t l sng v mc
n nh di truyn tng nhng hiu qu kinh t gim v ngc li. Do vy
phi kt hp gia cc yu t tm ra phng php ly mu ti u. Mt
nh sinh trng nui cy iu kin thch hp s pht trin thnh mt hay
nhiu chi v cc chi s pht trin thnh cy hon chnh c r y . Nu
xt v ngun gc cc cy ti sinh t nui cy nguyn liu ban u l m
phn sinh hoc nh sinh trng th c ba kh nng: cy pht trin t chi
nh (chi ngn); cy pht trin t chi nch ph ng; cy pht trin t chi
mi pht sinh. Tuy nhin trong thc t rt kh phn bit c chi ph ng
v chi mi pht sinh [28].
C 2 phng thc hnh thnh cy ti sinh t nui cy m phn sinh hoc
nh sinh trng. Cy ti sinh trc tip t chi nh hoc chi nch ph ng.
Phng thc ny ch yu cc i tng 2 l mm nh: khoai ty, thuc l,
cam, chanh, hoa cc, nhng c c cy 1 l mm nh da si, ma... cy ti
sinh qua giai on hnh thnh d hnh (protocorm), ch yu gp cc i
tng mt l mm nh: phong lan, da, hoa hu... Gn y phng thc ny
cng bt u c bt u c p dng c kt qu cc cy n qu v cy
lm nghip, trong c cy qu nh c ph, to, l, cy thng, b ... Tng
s c trn 30 chi khc nhau c nui cy thnh cng [2].
1.2.3.2. Ti sinh cy hon chnh t cc b phn khc ca cy

V t bo thc vt c tnh ton nng nn ngoi m phn sinh v nh sinh
trng l b phn d nui cy thnh cng, cc b phn cn li ca c th thc
vt u c th thc hin cho vic nhn ging in vitro c. Cc b phn l:
on thn cc i tng nh thuc l, cam, chanh,...; mnh l thuc l,
c chua, bp ci...; cung l Nacissus; cc b phn ca hoa nh sp l, la
m... v nhnh c hnh ti [2].
1.2.4. Quy trnh nhn ging in vitro
1.2.4.1. Chun b cy lm vt liu gc
V trong nui cy in vitro cy con s mang nhng c tnh v tnh trng
ca cy m ban u nn trong giai on ny cn chn lc cy m cn thn,
cy m thng l cy c nhiu c tnh u vit, kho, c gi tr kinh t cao.
Sau , chn c quan ly mu thng l m non, on thn c chi ng, l
non hoc hoa non... M chn nui cy thng l m c kh nng ti sinh
cao trong mi trng nui cy sch bnh, gi c cc c tnh sinh hc qu
ca cy m, t nguy c bin d. Tu theo iu kin, giai on ny c th ko
di 3-6 thng [19].
1.2.4.2. Thit lp h thng cy v trng
L giai on chuyn mu vt t ngoi vo mi trng nui cy to nguyn
liu sch bnh cho nhn ging, giai on ny c tin hnh theo cc bc:
Kh trng b mt mu vt v chun b cc mi trng nui cy.
Cy mu vt vo ng nghim hoc bnh nui cy c sn mi trng
nhn to (giai on ny l giai on cy mu in vitro).
Cc mu nui cy nu khng b nhim khun, nm, virus s c nui
trong phng nui cy vi iu kin nhit nh sng ph hp. Sau mt thi gian
nht nh, t mu nui cy bt u xut hin cc cm t bo hoc cc c quan
hoc cc phi v tnh. Giai on ny ph thuc vo tng i tng em nhn
ging, thng thng ko di t 2-12 thng hoc t nht 4 ln cy chuyn [19].
1.2.4.3. Nhn nhanh chi

y l giai on sn xut cy nhn ging quyt nh hiu qu ca qu
trnh nui cy m, cy c nhn nhanh theo nhu cu ca ngi nui cy. Khi
mu cy sch c to ra, t nhn c cc cm chi v cc phi v
tnh sinh trng tt trong qu trnh nui cy s bc vo giai on sn xut.
Ngi ta cn to ra tc nhn nhanh cao nht trong iu kin nui cy.
Thnh phn v iu kin mi trng cn ti u ho to c mc tiu
nhn nhanh. i vi mi trng nhn chi, ngi ta s dng cc cht kch
thch sinh trng thuc nhm cytokinin (BAP, kinetin) vi nng khc
nhau tu tng i tng cy. Quy trnh cy chuyn nhn nhanh chi
thng trong khong 1-2 thng tu loi cy. T l nhn nhanh khong 2-8 ln
sau mt ln cy chuyn. Nhn chung giai on ny thng ko di 10-36
thng. Giai on nhn nhanh chi t mt vi chi ban u khng nn ko di
qu lu trnh s hnh thnh bin d sma [19].
1.2.4.4. To r (to cy hon chnh)
Cc chi hnh thnh trong qu trnh nui cy c th pht trin r t sinh,
nhng thng thng cc chi ny phi cy chuyn sang mt mi trng khc
kch thch to r. i vi mi trng to r, ngi ta thng s dng cht
kch thch sinh trng thuc nhm auxin nh -NAA, IAA, IBA. Thng
thng giai on ny ko di 2-8 tun tu i tng. Khi cy c cc b
phn thn, l, r vi kch thch nht nh m bo cho sinh trng, pht trin
bnh thng ngoi t nhin, ngi ta mi tin hnh giai on tip theo l a
cy ra ngoi mi trng t nhin [19].
1.2.4.5. Chuyn cy ra t trng
y l giai on u cy c chuyn t iu kin v trng trong ng
nghim ra ngoi mi trng t nhin. Giai on ny quyt nh kh nng ng
dng ca quy trnh nhn ging in vitro. a s cc loi cy trng ch sau khi
chi ra r to thnh cy hon chnh vi kch thc nht nh mi c
10
hun luyn v chuyn ra ngoi vn m. Cy nui cy in vitro c sinh

trng v pht trin trong nhng iu kin ti u v nhit , m, pH, dinh
dng... V vy, trc khi a ra trng, ngi ta cn hun luyn cy thch
nghi vi iu kin t nhin. Qu trnh thch nghi vi iu kin bn ngoi ca
cy giai on u yu cu cn c chm sc c bit. V vy, cy c
chuyn t mi trng t bo ho hi nc sang vn m vi nhng iu
kin kh khn hn, nn vn m cn phi p ng cc yu cu: che cy non
bng nilon v c h thng phun sng cung cp m v lm mt cy; gi
th trng cy c th l t mn, hoc cc hn hp nhn to khng cha t,
mn ca v bt bin... Giai on ny i hi 4-16 tun [19].
1.3. MT S NGHIN CU NHN GING CHUI BNG K
THUT NUI CY IN VITRO
1.3.1. Cc nghin cu trong nc
Ngy nay phng php nui cy m t bo thc vt khng ch c s
dng trong nghin cu m cn c p dng rng ri vo thc tin chn ging,
khng ch cy ng cc m cn cy rau, hoa cy cnh v cy n qu.
Nui cy m v t bo thc vt phc v nhn ging cy trng trin
khai trn 20 nm nc ta. Nhn ging thng mi quy m ln t c
mt s cy trng nh nhn nhanh chui, nhn nhanh khoai ty sch bnh,
nhn nhanh cc ging ma nhp ni Quy trnh cng ngh nhn nhanh ging
chui v ging ma c B Nng nghip v Pht trin Nng thn cng
nhn v p dng [2].
Cc nghin cu v nhn ging chui trc y ch ch trng cc bin
php k thut nhn bng c v tch chi. Nhng k thut ny hin cn c
p dng kh ph bin nhiu vng min v nht l quy m sn xut nh.
K thut nhn ging v tnh chui bng phng php in vitro nc ta
cng thu c mt s kt qu sau:
on Th i Thuyn v cng s (1993) a ra quy trnh nhn ging
11
chui bng phng php nui cy m bao gm 5 cng on chnh sau: a

mu vo nui cy to v nhn nhanh chi chui to r cy m
chui trong vn m bu chui v trng ra rung sn xut. V cng cho
bit cy chui nui cy m vn m 60 - 70 ngy (lung m 30 - 40
ngy v bu t 30 ngy) th c xut vn, khi cy cao 40 - 50 cm [21].
Theo Nng Vnh v cng s (1994) cho bit t l ti sinh dao ng
t 68,42 - 92,31% do b chi phi bi bn cht di truyn ca ging chui v
cc cht b sung vo mi trng nui cy. H s nhn ca chui Tiu cao
nht khi b sung BAP t 7 - 9 mg/lt. Nc da khng biu hin nh hng
n chui Tiu nhng c nh hng tt ti h s nhn ca chui Rng
lng 10%, khi c mt BAP vi lng 7 mg/lt [26].
Theo Nguyn Quang Thch v cng s (1995) cho bit hon ton c
th s dng phng php in vitro nhn nhanh cy chui, vt liu nui cy
tt nht cho mc ch nhn nhanh l cc m chi nh v chui c th s
dng k thut bc b khng cn kh trng, mi trng thch hp cho qu
trnh khi ng pht sinh chi ban u l mi trng MS + (5 - 7) ppm BA,
mi trng nhn nhanh tng t nh mi trng khi ng nhng sau nhiu
ln cy chuyn cn gim hm lng BA thm tr ti 0 ppm v c th b sung
nc da l 10%. Cn mi trng ra r tt nht l MS + 0,2 g/lt than hot
tnh v cng nhn xt vic a cy chui in vitro ra vn m v h thu l
hon ton thun li, t l sng t 100% trn c 3 gi th nghin cu l: ct,
t tht nh, t + ct + phn chung [17].
Theo Nng Vnh v Cng s (1995), cy chui nui cy m cn
a ra lung gim gm 3 lp: lp di l t dy 5cm, lp gia l phn
chung i trn vi t ct pha t l 1:1 dy 7cm, lp trn cng l ct vng 5 -7
cm; mt gim l 300 - 400 cy/m2; thi gian lung gim l 30 ngy. Sau
, chui c a ra bu t c kch thc 7 - 10 10 - 15 cm; thi gian
bu t t 45 - 60 ngy, ma ng rt c th lu hn. Nh vy, tng thi gian
12
vn m l 2,5 - 3 thng hoc lu hn na. Cng c th a thng cy non

ra bu t khng qua lung gim. t ng bu c thnh phn: phn hu c
vi sinh + ct + t ph sa hoc t pha ct (t l 1:1:1) l tt nht [26].
Theo Phm Kim Thu v ng Th Vn (1997), t nghin cu hon thin
quy trnh sn xut cy ging chui bng phng php nui cy m, Tc gi kt
lun mi trng nui cy chui l MS (1962) c b sung 1 ppm Thiamin HCl
lm tng kh nng ti sinh chi chui, nn gi th ra cy cho t l sng cao
nht l 1/3 t + 1/3 phn hu c + 1/3 ct en v thi v ra cy thch hp t
thnh 4 n thng 10. ng dng kt qu ny sn xut c hng triu cy
ging cung cp cho cc tnh pha Bc (Nam nh, Thi Bnh).
1.3.2. Cc nghin cu ca nc ngoi
Chui l i tng cy n qu c hnh thc sinh sn v tnh vi phng
thc nhn ging truyn thng l s dng chi nch lm ging trng nhng
th h k tip.V vy, s dng k thut nui cy m t bo nhn ging chui
c nghin cu, ng dng t rt lu nhiu nc trn th gii nh
Malayxia, Asutralia, Php, Trung Quc v gp 1 phn ng k phc v
ngnh sn xut chui xut khu.
Theo Reuveni (1986), k thut nui cy in vitro chui c mt s u
im sau :
- Nhn c s lng ln ging nh cy trng ban u xc nh tnh trng.
- Cht lng cy ging hon ton sch bnh, trnh c nhng su
hi gy ra qua ngun t (tuyn trng) v vy tit kim c chi ph ha
cht do s l t.
- Cy nui cy m c th trng mt v vi mc thm canh cao, thi
gian sinh trng ngn, c iu khin c thi gian thu hoch.
- T l cy sng cao trn iu kin ng rung (>98%), kh nng sinh
trng nhanh hn cy c ngun gc t chi nch.
- Cy ging in vitro pht trin ng u, ra hoa ng lot v thi gian
thu hoch ngn.
13
CHNG 2. VT LIU V PHNG PHP NGHIN CU

2.1. VT LIU, A IM V THI GIAN NGHIN CU
2.1.1. Vt liu thc vt
Chi chui Tiu Hng trong giai on in vitro do phng Cng ngh t
bo thc vt - Vin Di truyn Nng nghip cung cp.
2.1.2. Ha cht v thit b
* Ha cht
Cc cht kch thch sinh trng, ng saccarose, agar, nc da,...vv.
Nui cy c tin hnh trn nn mi trng MS, B5 v mi trng
Phytamax (Bng thnh phn mi trng theo ph lc 01nh km).
* Dng c
Bnh tam gic, pipet, cc thy tinh nh mc.
Bng, giy lm nt, giy thm.
Bnh tam gic 250ml.
B cy gm: dao cy, que cy, a cy
Bung cy v trng (Biological Safety Cabinets) ca hng Nuarie, M.
Ni kh trng (Auto Clave) ca hng ToMy, Nht bn.
Bnh phun ti cy.
Panh, ko, chu nha, r nha, gi th trng cy
2.1.2. a im v thi gian nghin cu.
2.1.2.1. a im
Cc ni dung nghin cu c tin hnh ti phng th nghim nui cy
m v vn thc nghim S Khoa hc & Cng ngh Bc Ninh.
2.2.2.2. Thi gian: T 01/06/2012 n 03/2013
14
2.2. NI DUNG NGHIN CU

- Nghin cu nh hng ca mi trng c bn MS, B5 v Phytamax
- Nghin cu nh hng ca cht kch thch sinh trng thuc nhm
cytokinin n s pht sinh chi v s sinh trng ca chui Tiu Hng.
- Nghin cu s nh hng ca cht kch thch sinh trng thuc nhm
auxin n pht sinh r v to cy hon chnh.
- Nghin cu nh hng ca t hp cc cht kch thch sinh trng thuc
nhm auxin v nhm cytokinin ln s sinh trng ca chui Tiu Hng.
- Nghin cu tm ra loi gi th thch hp nht cho ra cy chui Tiu
Hng (t, ct, tru hun ).
2.3. PHNG PHP NGHIN CU
2.3.1 Phng php b tr th nghim
Cc th nghim c b tr mt cch ngu nhin hon ton vi 3 ln
nhc li, mi ln nhc 30 bnh, mi bnh 5 mu.
2.3.2. Phng php pha mi trng v nui cy
2.3.1.1. Pha mi trng nui cy
Pha mi trng c vi thnh phn v nng cc cht ph hp. Mi
trng c y mui khong, cc cht hu c, vitamin Tt c cc ha
cht phi c tan u, khng kt ta. Mi trng c b sung cht n l
thch lm gi khng qu rn hay qu mm khi cy mu vt c d
dng. Kh trng mi trng theo phng php Pasteur.
2.3.1.2. Phng php nui cy
Trc khi tin hnh nui cy, cn kh trng box cy bng n UV
trong thi gian 30-60 pht, kh trng b cy bng cch t trn ngn la n
cn, sau ngui.
15
Cm chi chui c ly t bnh ging ra, tch thnh tng cm nh cc

cm chi ny c cy vo mi trng nhn ging. B tr th nghim vi cc
cng thc mi trng ty tng mc ch nghin cu. Bnh nui cy c t
trong nh nui cy vi cng nh sng 2000lux, thi gian chiu sng
10/24 gi, nhit 25-270C.
2.3.2. Phng php ra cy
Khi cy con c b r khe, l xanh tt c tin hnh hun luyn a
cy trng ngoi t nhin bng cch cc bnh cy ra ni c nh sng khuch
tn 2-3 ngy trc khi a cy ra khi bnh.
Kh nng sng ca cy khi chuyn t trong ng nghim ra vn m
quyt nh hiu qu kinh t ca phng php nhn ging in vitro. Do , mt
quy trnh ra cy t hiu qu cao c ngha rt ln.
2.3.2.1. a cy ra ngoi mi trng
Cc bnh cy c m nt, cho nc vo ngm 10-15 pht, sau lc
nh cho thch ri ra khi cy, dng panh nh nhng gp cy ra khi bnh, c
gng cy c nguyn vn khng b dp nt.
Ngm cy trong chu nc sch, sau ra di vi nc chy cho ht
thch bm vo b r ca cy.
Nhng cy tiu chun c trng trn gi th t, ct, tru hun, t
+ tru hun (1:1), ct + tru hun (1:1), t + ct + tru hun (1:1:1)
Sau khi trng, phun nc nh nhng cho cht gc cy.
2.3.2.2. Chm sc cy con
Trong 2-3 ngy u ti nc l bng cch phun sng 3 gi/ln. Tri
rt, kh hanh phi che nilon trnh rt v trnh thot nc nhanh. Nilon che
phi c ra mt s l thng cho thong kh. Sau 3 ngy c th b nilon che,
cy ra ni nh sng, thong kh nhng khng gi, trnh nng ma trc
tip. Ti nc bng bnh phun 2-3 ln/ngy. Sau 3-4 tun cy sng ra r
mi, l mi l c th a ra vn m.
16
2.3.3. Phng php b tr th nghim

2.3.3.1 Ni dung 1: Nghin cu nh hng ca mi trng n s sinh
trng ca chui Tiu Hng.
Th nghim 1: Nghin cu nh hng ca mi trng MS, B5 v mi trng
Phytomax n s sinh trng ca chui Tiu Hng.
- Cc mi trng theo bng 3.1 c b sung saccharose 30g/l + agar
6,5g/l + nc da 100ml/l, pH mi trng ~ 5,8.
Cng thc nh hng ca mi trng n s pht sinh chi v cm chi
Cng thc
Mi trng
CT1
MS
CT2
Phytamax
CT3
B5
2.3.3.2. Ni dung 2: Nghin cu nh hng ca cht kch thch sinh trng

thuc nhm cytokinin n s pht sinh chi v s sinh trng ca chui Tiu
Hng.
Cc th nghim c tin hnh nui cy trn mi trng tt nht th
nghim 1 c b sung ,6,5g agar + 30g ng + 100ml nc da sau b
sung ring r hoc kt hp cc cht iu tit sinh trng cc nng khc
nhau theo cc cng thc th nghim.
2.3.3.2.1. Th nghim 2: Nghin cu nh hng ca BAP ti s pht sinh chi
v to cm chi chui Tiu Hng.
- Mi trng nn: Mi trng tt nht ni dung 1, b sung saccharose
30g/l + agar 6,5g/l + nc da 100ml/l, pH mi trng ~ 5,8, b sung BAP
theo bng sau.
17
Cng thc nh hng ca BAP n s pht sinh chi v to cm chi
chui Tiu Hng

Cng thc
CT1
CT2
CT3
CT4
CT5
CT6
Nng BAP (mg/l)

0
1
3
5
7
9
2.3.3.2.2. Th nghim 3: Nghin cu nh hng ca BAP ti s pht sinh chi

v to cm chi chui Tiu Hng.
30g/l + agar 6,5g/l + nc da 100ml/l, pH mi trng ~ 5,8, b sung BAP
theo bng sau.
Cng thc nh hng ca kinetin n s pht sinh chi v to cm chi
chui Tiu Hng

Cng thc
CT1
CT2
CT3
CT4
CT5
CT6
Nng kinetin (mg/l)

0
1
3
5
7
9
2.3.3.3. Ni dung 3: Nghin cu nh hng ca t hp cc cht kch thch

sinh trng thuc nhm auxin v nhm xytokinin ln s sinh trng ca
chui Tiu Hng.
2.3.3.3.1. Th nghim 4: Nghin cu nh hng ca t hp cht kch thch
sinh trng BAP v -NAA n s sinh trng ca chui Tiu Hng.
30g/l + agar 6,5g/l + nc da 100ml/l, pH mi trng ~ 5,8, b sung BAP v
NAA theo bng sau.
18
nh hng ca t hp cht kch thch sinh trng BAP v -NAA ti s

sinh trng ca chui Tiu Hng.
Cng thc
Nng NAA (mg/l)
Nng nhm BAP (mg/l)
CT1
B
0
CT2
B
0,2
CT3
B
0,4
CT4
B
0,6
CT5
B
0,8
B l nng BAP tt nht th nghim ni dung 2

sinh trng BAP v IAA n s sinh trng ca chui Tiu Hng.
IAA theo bng sau.
Cng thc
Nng nhm BAP (mg/l)
Nng IAA (mg/l)
CT1
B
0
CT2
B
0,2
CT3
B
0,4
CT4
B
0,6
CT5
B
0,8

sinh trng BAP v IBA n s sinh trng ca chui Tiu Hng.
IBA theo bng sau.
Cng thc
Nng IBA (mg/l)
Nng nhm BAP (mg/l)
CT1
B
0
CT2
B
0,2
CT3
B
0,4
CT4
B
0,6
CT5
B
0,8
19
2.3.3.3.4.Th nghim 7: Nghin cu nh hng ca t hp cht kch thch sinh

trng Kinetin v -NAA n s sinh trng ca chui Tiu Hng.
30g/l + agar 6,5g/l + nc da 100ml/l, pH mi trng ~ 5,8, b sung kinetin
v NAA theo bng sau.
nh hng ca t hp cht kch thch sinh trng kinetin v -NAA ti

s sinh trng ca chui Tiu Hng.
Cng thc
Nng kinetin (mg/l)
Nng NAA (mg/l)
CT1
CT2
0,2
CT3
0,4
CT4
0,6
CT5
0,8
B l nng Kinetin tt nht th nghim ni dung 2

sinh trng BAP v IAA n s sinh trng ca chui Tiu Hng.
30g/l + agar 6,5g/l + nc da 100ml/l, pH mi trng ~ 5,8, b sung kinetin
v IAA theo bng sau.
nh hng ca t hp cht kch thch sinh trng kinetin v IAA ti s

Cng thc
Nng kinetin (mg/l)
Nng IAA (mg/l)
CT1
CT2
0,2
CT3
0,4
CT4
0,6
CT5
0,8
B l nng kinetin tt nht th nghim ni dung 2
20
2.3.3.3.6.Th nghim 9: Nghin cu nh hng ca t hp cht kch thch sinh

trng kinetin v IBA n s sinh trng ca chui Tiu Hng.
IBA theo bng sau.
nh hng ca t hp cht kch thch sinh trng kinetin v IBA ti s

Cng thc
Nng kinetin (mg/l)
Nng IBA (mg/l)
CT1
CT2
0,2
CT3
0,4
CT4
0,6
CT5
0,8
B l nng kinetin tt nht th nghim ni dung 2
2.3.3.4. Ni dung 4: Nghin cu s nh hng ca cht kch thch sinh

trng thuc nhm auxin n pht sinh r v to cy hon chnh.
2.3.3.4.1. Th nghim 10: nh hng ca cht kch thch sinh trng -NAA
n s pht sinh r ca chui Tiu Hng.
30g/l + agar 6,5g/l + nc da 100ml/l, pH mi trng ~ 5,8, b sung NAA
theo bng sau.
nh hng ca -NAA ti s pht sinh r chui Tiu Hng
Cng thc
CT1
CT2
CT3
CT4
CT5
CT6
Nng NAA (mg/l)

0
0,2
0,4
0,6
0,8
1,0
21
2.3.3.4.2. Th nghim 11: nh hng ca cht kch thch sinh trng IAA n
s pht sinh r ca chui Tiu Hng
30g/l + agar 6,5g/l + nc da 100ml/l, pH mi trng ~ 5,8, b sung IAA
theo bng sau.
nh hng ca IAA ti s pht sinh r chui Tiu Hng
Cng thc
CT1
CT2
CT3
CT4
CT5
CT6
Nng IAA (mg/l)

0
0,2
0,4
0,6
0,8
1,0
2.3.3.4.3. Th nghim 12: nh hng ca cht kch thch sinh trng IBA n
s pht sinh r ca chui Tiu Hng.
30g/l + agar 6,5g/l + nc da 100ml/l, pH mi trng ~ 5,8, b sung IBA
theo bng sau.
nh hng ca IBA ti s pht sinh r chui Tiu Hng
Cng thc
CT1
CT2
CT3
CT4
CT5
CT6
Nng IBA (mg/l)

0
0,2
0,4
0,6
0,8
1,0
2.3.3.5. Ni dung 5: Nghin cu tm ra loi gi th thch hp nht cho ra cy

chui Tiu Hng.
Th nghim 13: Nghin cu nh hng ca gi th t, ct, tru hun thch
hp nht cho ra cy chui Tiu Hng.
Khi cy con c b r khe, l xanh tt c tin hnh hun luyn a
22
cy trng ngoi t nhin bng cch cc bnh cy ra ni c nh sng khuch

tn 2-3 ngy trc khi a cy ra khi bnh.
Sau cy nui cy m c trng trn gi th t, ct, tru hun
theo bng sau:
Cng thc
Gi th
T l
CT1
100%
CT2
Ct
100%
CT3
Tru hun
100%
CT4
t + tru hun
1:1
CT5
Ct + tru hun
1:1
CT6
t + ct + tru hun
1:1:1
2.3.4. Phng php thu thp s liu v x l s liu

2.3.4.1. Thu thp s liu
Cc o m trong phng th nghim:
- S lng chi ban u, s lng chi thu c.
- Chiu cao trung bnh ca chi.
- S r trung bnh chi.
2.3.4.2. Cc ch tiu theo di v tnh ton
Chi thu c
+ H s nhn chi (ln) =
Chi ban u
+ Chiu cao trung bnh ca chi(cm) =

+ S r trung bnh chi =
Chiu cao chi
S chi
S r thu c
S chi thu c
chiu di r thu c
+ Chiu di trung bnh r =

S r thu c
23
- Bng cm quan nh gi 3 mc:

+ Sinh trng tt (+++): chi mp, cht lng chi ng u, thn chi
va phi, l xanh m.
+ Sinh trng trung bnh (++): chi hi cao, l mu xanh nht, cm
chi pht trin hn mc yu.
+ Sinh trng km (+): Cc cm chi pht trin khng ng u,chi gy.
2.3.5. Phng php tnh ton kt qu
Sau khi tin hnh nui cy v thu thp s liu, s liu thu c s c
tnh ton trn phn mm Excel chng trnh thng k sinh hc IRRISTAT 4.0
24
CHNG 3. KT QU V THO LUN

3.1. So snh nh hng ca mi trng nui cy MS, B5 v Phytamax ti
s pht sinh chi v to cm chi chui Tiu Hng.
Mi trng l iu kin ti cn thit, l yu t cho s phn ha t bo
v c quan trong nui cy t quyt nh n s lng v cht lng chi.
Mi mt loi vt liu khc nhau c nhng i hi khc nhau v thnh phn
mi trng. Mi trng c cha thnh phn v hm lng dinh dng khc
nhau to nn hiu qu nui cy khc nhau ln qu trnh to chi v cm chi.
C nhiu cng trnh nghin cu m chui ca mt s tc gi nh: Ma v Shii
(1972), Kawit Wanichkul v cng s (1993), Nng Vnh v Cng s
(1995), Nguyn Quang Thch v cng s (1995), Phm Kim Thu v ng
Th Vn (1997)... u s dng mi trng MS nui cy m cy chui. Tuy
nhin nghin cu mi trng nui cy cy chui Tiu Hng th rt t tc gi
nghin cu. Do nghin cu nh hng ca mi trng n s hnh
thnh chi v cm chi trong nui cy m cy chui Tiu Hng chng ti tin
hnh nghin cu trn 3 loi mi trng: MS c bn, B5 v Phytamax b sung
saccharose 30g/l + agar 6,5g/l + nc da 100ml/l , pH mi trng ~ 5,8
nhm tm ra mi trng ti u cho s pht sinh chi chui Tiu Hng, vi
mi cng thc 30 mu, 3 ln nhc li. Kt qu thu c sau 1 tun, 2 tun, 3
tun nui cy tng hp th hin qua bng 3.1.
Bng 3.1. So snh nh hng ca mi trng MS, B5 v Phytamax ti s
Ch tiu so snh
T l m to chi (%)
S chi trn m
Chiu cao chi (cm)
T l m to cm chi (%)
MS
Sau 3 tun
34,44
1,470,15
1,690,05
28,89
Phytamax
B5
31,85
1,190,16
1,530,07
24,04
29,26
1,170,12
1,430,08
19,35
25
Kt qu bng s liu 3.1 cho thy, trn c 3 loi mi trng m chng

ti tin hnh th nghim u pht sinh chi v cm chi nhng cc mi trng
khc nhau th t l to chi v cm chi khc nhau. nh hng ca mi
trng MS ti s pht sinh chi v to cm chi ca chui Tiu hng cao hn
so vi mi trng Phytamax v mi trng B5. C th, mi trng MS, t
l m sng, t l m to chi v t l m to cm chi sau 3 tun ln lt t
80,63%; 34,44%; 28,89%. Cc ch tiu so snh trn mi trng Phytamax
thp hn so vi mu nui cy trn mi trng MS sau 3 tun (sau 3 tun ln
lt t 61,48%; 31,85%; 24,04%) v mi trng B5 (sau 3 tun ln lt t
58,52%; 29,26%; 19,35%). Theo cc nh khoa hc mi trng Phytamax v
mi trng B5 thch hp cho loi phong lan, cn mi trng MS c s
dng rng ri hn c v thch ng rng cho nhiu loi cy nui vy invitro.
Kt qu nghin cu ca chng ti cng cho thy tnh u vit ca mi trng
MS trong nui cy m cy chui Tiu Hng to chi v cm chi. Do vy,
qua nghin cu v quan st kt qu, chng ti s dng mi trng MS, b
sung saccharose 30g/l + agar 6,5g/l + nc da 100ml/l cho cc nghin cu
tip theo.
Kt qu trn cng trng lp vi nhn xt ca Nguyn Th Liu v cng
s (2011) khi cho rng: mc d c rt nhiu cng thc mi trng nui cy
khc nhau dng nui cy cc loi thc vt khc nhau nhng cng thc pha
mi trng MS c s dng nhiu nht, thng ch thay i cc thnh phn
khong a lng.
26
Hnh 3.1. So snh nh hng ca mi trng MS, Phytamax v B5 ti s

A. M chui sau 3 tun
(MS)
B. M chui sau 3 tun C. M chui sau 3 tun

(Phytamax)
(B5)
3.2. nh hng ca cc cht kch thch sinh trng thuc nhm

cytokinin ti s pht sinh chi v to cm chi chui Tiu Hng.
3.2.1. nh hng ca BAP ti s pht sinh chi v to cm chi chui
Tiu Hng.
Mi trng nui cy s dng trong th nghim ny c cc thnh phn
nguyn t a lng, vi lng, ngun cacbon, vitamin, agar, nc da tng
t nhau, ch khc nhau v thnh phn cc cht iu tit sinh trng. Cht iu
ha sinh trng l yu t quan trng nht quyt nh n kt qu nui cy
cng nh qu trnh pht sinh chi. Mi loi m, hay mc ch khc nhau th
s dng cht iu ha sinh trng khc nhau. Mc tiu ca nghin cu l tm
ra mi trng tt nht v cc cht iu tit sinh trng b sung vo hp l
nht thu c s lng v cht lng chi tt nht. V vy chng ti lm
th nghim nghin cu nh hng ca cht iu tit sinh trng n s pht
sinh chi v cm chi. Cht iu tit sinh trng b sung vo l BAP. Cht
ny thuc nhm cytokinin, l nhm c s dng rng ri nht trong k thut
27
nui cy m hin nay. Tc dng ch yu ca BAP l kch thch s phn chia

t bo, thc y s hot ng ca chi. Nng s dng khc nhau tu i
tng thc vt nui cy.
Theo nhiu nghin cu cho thy, BAP thng c s dng vi
nng thay i t 1 - 5mg/l l thch hp cho nhiu loi m cy. cc
nng cao hn hoc thp hn u biu hin hiu qu kch thch km,
dn n s to chi v sinh trng ca chi gim. Vi nng BAP cao
s hot ha s hnh thnh chi bt nh [21]. Da trn nghin cu ca mt
s tc gi trn i tng khc nhau nh: nui cy m phn sinh ging lan
[29], nui cy m phn sinh ging chui Laba [25], chng ti tin hnh
nghin cu thm d tm ra mi trng ti u cho s pht sinh chi v
cm chi ca chui Tiu hng bng cch s dng mi trng i chng
khng c cc cht kch thch sinh trng (mi trng MS + saccharose
30g/l + nc da 100ml/l + agar 6,5g/l) v mi trng c b sung cc
cht kch thch sinh trng cc nng khc nhau (b sung BAP cc
nng t 1,0mg/l n 9,0mg/l). Th nghim c tin hnh vi 3 ln
nhc li, mi cng thc 30 mu. Theo di sau 1, 2, 3 tun nui cy, quan
st v ghi nhn c kt qu qua bng s liu 3.2.
T kt qu bng 3.2 chng ti thy sau 3 tun nui cy tt c cc cng
thc u pht sinh chi. khi b sung vo mi trng nui cy cc nng
BAP khc nhau th kh nng pht sinh chi c s khc nhau. mi trng
i chng sau 3 tun, t l m to chi t 31,85% . Mi trng nui cy b
sung BAP nng 5mg/l cho hiu qu cao nht. Sau 3 tun nui cy, t l m
to chi t 58,89% .Nh vy, sau 3 tun nui cy, t l m to chi mi
trng b sung BAP 5mg/l tng ln 10.48%, cn mi trng i chng ch
tng ln 1,85%. Nh vy, t l m to chi mi trng b sung BAP 5mg/l
tng 5,66 ln so vi t l m to chi ca mi trng i chng.
28
Bng 3.2. nh hng ca BAP ti s pht sinh chi v to cm chi

chui Tiu Hng.
Cng
BAP
thc th
nghim
T l m
to chi
(mg/l)
S chi/m
(%)
Chiu cao
Cht lng
chi (cm)
chi
Sau 3 tun
C
31,85
1,67
3,08
++
CT1
40,00
3,62
2,56
++
CT2
41,48
3,85
2,72
++
CT3
58,89
4,58
1,49
+++
CT4
39,26
4,15
2,59
++
CT5
36,30
4,03
2,67
++
CV%
0,5
3,0
2,7
LSD
0,35
0,19
0,12
Ghi ch: (++):Chi pht bnh thng

(+++):Chi xanh, mp, pht trin cn i, l to xanh m
mi trng i chng tuy khng b sung cc cht kch thch sinh trng,
song trong mi trng c b sung nc da. Trong nc da c cha cc cht
kch thch sinh trng thuc nhm auxin v cytokinin vi nng thp nn s
pht sinh chi v cm chi thp. Mi trng ti u c b sung BAP 5mg/l v c
s cn i gia cc thnh phn dinh dng nn s pht sinh chi v cm chi cao.
Do vy, t hiu qu cao trong cc nghin cu tip theo, chng ti
b sung BAP vi nng 5mg/l vo mi trng nui cy. Kt qu trn cng
ph hp vi kt lun ca Nguyn Quang Thch v cng s (1995) khi nghin
cu v nh hng ca BAP ti s sinh trng chui. Tc gi cho rng, b
sung 5 - 7mg/l BAP vo mi trng nui cy cho hiu qu sinh trng tt.
29
3.2.2. nh hng ca kinetin ln s pht sinh chi v to cm chi chui

Tiu Hng.
Kinetin l sn phm c pht hin u tin khi Skoog (1950) tin
hnh th nghim chit xut axit nucleic. Trong c th sng khng c kinetin
tn ti. c to ra bng cch phn lp t ch phm ADN c hoc axit
nucleic mi sau khi hp nhit cao hoc un si, kinetin kch thch s
pht sinh chi ca thc vt. Tuy nhin, khi phi hp x l kinetin cng cc
cht kch thch sinh trng thuc nhm auxin t l nng thch hp th s
kch thch qu trnh phn chia t bo [2].
Da trn nhng c tnh ca kinetin, chng ti tip tc nghin cu nh
hng ca kinetin ln s pht sinh chi v to cm chi chui Tiu Hng
bng cch b sung vo mi trng nui cy cc thang nng t 1,0mg/l
9,0mg/l. Kt qu thu c th hin bng 3.3.
Qua bng s liu, s pht sinh chi v cm chi chui Tiu hng t
hiu qu cao nht khi b sung kinetin 7,0mg/l. Sau 3 tun nghin cu, mi
trng b sung kinetin 7,0mg/l, t l m to chi t 63,33% (cao gp 2,95
ln so vi mi trng i chng khng b sung kinetin (21,48%).
Sau 3 tun nghin cu, mi trng c b sung kinetin 7,0mg/l, s
chi/m t 4,13 chi; chiu cao ca chi t 3,64cm. T l trn cao hn so
vi nghin cu trn mi trng i chng khng b sung kinetin (s chi/m
t 1,72 chi; chiu cao ca chi t 1,39cm). Nh vy, vic b sung kinetin
vo mi trng nui cy cho hiu qu cao so vi mi trng i chng.
t hiu qu cao khi nghin cu, chng ti b sung vo mi trng
nui cy kinetin 7,0mg/l, da trn nn mi trng MS + saccarose 30g/l +
agar 6,5 g/l + nc da 100ml/l.
Khi so snh mi trng nui cy c b sung BAP 5mg/l v mi trng
nui cy b sung kinetin 7,0mg/l, ti nhn thy sau 3 tun, t l m to chi mi
trng c kinetin (63,33%) cao hn so vi mi trng c BAP (58,89%). iu
cho thy b sung kinetin 7,0mg/l vo mi trng nui cy chui Tiu hng l t
hiu qu nhn chi ti u. Tuy nhin, mc tiu ca ti hng ti khng ch l
30
tm ra mi trng nhn ging ti u cho chui Tiu hng, m cn l gi tr kinh t

em li khi a vo sn xut i tr. Chnh v vy, nu xt trn gc kinh t, b
sung BAP 5mg/l vo mi trng nui cy mang li hiu qu ti u.
Bng 3.3. nh hng ca kinetin ti s pht sinh chi v to cm chi
chui Tiu Hng.
Cng thc
th nghim
Kinetin
(mg/l)
C
CT1
CT2
CT3
CT4
CT5
CV%
LSD
0
1,0
3,0
5,0
7,0
9,0
T l m
S chi/m
to chi(%)
Sau 3 tun
31,48
1,72
46,12
3,58
47,41
3,83
52,59
3,87
63,33
4,13
67,41
3,87
0,8
2,3
0,72
0,14
Chiu cao
chi (cm)
Cht lng chi
3,64
2,47
2,52
1,46
1,39
2,45
2,6
0,11
++
++
++
+++
++
++
Ghi ch: (++):Chi pht bnh thng

(+++):Chi xanh, mp, pht trin cn i, l to xanh m
B
A
Hnh 3.2. nh hng ca kinetin ti s pht sinh chi v to cm chi
chui Tiu Hng
A. M chui sau 3 tun (C)
B. M chui sau 3 tun (kinetin 7,0mg/l)
31
3.3. nh hng ca cc cht kch thch sinh trng thuc nhm auxin ti
s pht sinh r chui Tiu Hng.
3.3.1. nh hng ca cht kch thch sinh trng -NAA n s pht sinh
r ca chui Tiu Hng.
-NAA l cht iu ha sinh trng thuc nhm auxin, c tng hp
bng con ng ha hc, c hot tnh sinh l nh mt auxin t nhin, c tc
dng nhiu mt ln qu trnh sinh trng ca t bo, hin tng u th ngn,
tnh nh hng ca thc vt, c bit l kch thch to r. -NAA c s
dng nng t 0,1mg/l - 2,0mg/l v c hiu qu sinh l nng thp.
Nghin cu nh hng ca -NAA ti s sinh trng ca chui Tiu hng,
chng ti s dng cc thang nng t 0,2mg/l - 1,0mg/l. Kt qu thu c
th hin trong bng 3.4.
Bng 3.4. nh hng ca -NAA ti s pht sinh r chui Tiu Hng
Cng
thc th
nghim
-NAA
(mg/l)
T l
cy ra
S r/cy
r (%)
Chiu di
r (cm)
Mu sc r Mu sc l
Sau 3 tun
C
71,19
4,97
4,99
Xm
Xanh BT
CT1
0,2
82,09
6,06
5,17
Xm
Xanh BT
CT2
0,4
91,48
6,27
5,94
Trng
Xanh BT
CT3
0,6
94,07
6,48
5,27
Trng xm
Xanh BT
CT4
0,8
75,16
6,24
5,19
en
Xanh BT
CT5
1,0
81,07
6,14
5,10
en
Xanh nht
CV%
0,1
1,0
1,0
LSD
0,12
0,10
0,98
Sau 3 tun nghin cu, t l cy ra r nng 0,6mg/l -NAA t

94,07%; t l s r/cy t 6,48. nng 0,4mg/l -NAA, t l cy ra r v
t l s r/cy ln lt t 91,48% v 6,27. S sai khc 2 nng trn
32
khng c s sai khc ng k. Do , tit kim chi ph trong qu trnh

nghin cu, ti b sung vo mi trng nui cy -NAA 0,4mg/l.
3.3.2. nh hng ca cht kch thch sinh trng IAA n s pht sinh r
ca chui Tiu Hng.
IAA l dng auxin ch yu, quan trng nht tt c cc loi thc vt,
k c thc vt bc thp v thc vt bc cao. IAA c hnh thnh lin tc
trong nh sinh trng ca thn v r cy v c tc dng tt n cc qu trnh
sinh trng ca t bo, hot ng ca tng pht sinh, s hnh thnh r... Trong
nghin cu ny, b sung IAA vo mi trng to r vi hm lng t 0,2mg/l
n 1,0mg/l khng nhng kch thch s hnh thnh r m cn c tc dng tt
i vi s pht trin ca l chui Tiu Hng. Kt qu thu c nh sau:
mi trng i chng khng b sung IAA th sau 3 tun nghin cu,
t l cy ra r mi trng b sung IAA 0,6mg/l cho kt qu cao nht l
95,56%, gp 1,48 ln so vi mi trng i chng (64,44%); t l s r/cy
t 5,95 cao nht trong s cc nng IAA b sung vo mi trng. Ngoi
ra, cc ch tiu v chiu di r v s r/cy mi trng c b sung IAA
0,6mg/l u cho kt qu tt nht
Bng 3.5. nh hng ca IAA ti s pht sinh r chui Tiu Hng
Cng thc
th nghim
IAA
(mg/l)
T l cy
ra r (%)
S r/cy
Chiu di
l (cm)
Mu sc r Mu sc l
Sau 3 tun
C
0,0
64,44
4,61
5,22
Xm
Xanh nht
CT1
0,2
72,96
4,87
5,41
Xm
Xanh BT
CT2
0,4
87,04
4,92
5,45
Trng
Xanh BT
CT3
0,6
95,56
5,95
5,69
en
Xanh BT
CT4
0,8
83,33
4,93
5,28
Trng xm
Xanh BT
CT5
1,0
82,22
4,84
5,33
en
Xanh nht
CV%
0,5
1,1
0,8
LSD
0,76
0,95
0,74
33
Nh vy, mi trng nui cy da trn nn MS c bn + saccharose

30g/l + agar 6,5g/l + nc da 100ml/l, b sung IAA 0,6mg/l cho hiu qu
cao nht thc y s ra r in vitro ging chui Tiu Hng.
3.3.3. nh hng ca cht kch thch sinh trng IBA n s hnh thnh
r ca chui Tiu Hng.
Sau khi nghin cu nh hng ca IAA, ti tip tc nghin cu nh
hng ca IBA ti s pht sinh r in vitro ging chui Tiu Hng. Cng
thuc cht kch thch sinh trng auxin, IBA c vai tr tng t nh IAA i
vi s sinh trng v pht trin ca thc vt.
c nhiu nghin cu v nh hng ca IBA i vi s sinh trng
v pht trin i vi thc vt ni chung v chui ni ring. chng ti tip tc
nui cy in vitro chui Tiu hng trong mi trng MS c bn + saccharose
30g/l + agar 6,5g/l + nc da 100ml/l, b sung IBA c nng t 0,2mg/l
n 1,0mg/l.
Kt qu bng 3.6 cho thy, b sung IBA vi cc nng khc nhau t
0,2mg/l n 1,0mg/l cc cng thc mi trng u thy c s pht sinh r
ca chui Tiu hng, song gia cc cng thc khc nhau c s khc nhau v
cc ch s: t l cy ra r, s r/cy, mu sc r v mu sc l.
B sung IBA vi nng 0,8mg/l cho t l cy ra r cao nht t
95,19% vi thi gian 3 tun, s r/cy cao nht t 5,64 tng ng vi thi
gian 3 tun. Theo di cc ch tiu khc nh chiu di r v mu sc l gia
cc cng thc khng c s sai khc ng k.
Qua cc th nghim v nh hng ca IBA ti s pht sinh r ca chui
tiu hng cho thy, mi trng thch hp nht l MS + saccharose 30g/l +
agar 6,5g/l + nc da 100ml/l + 0,8mg/l IBA.
34
Bng 3.6. nh hng ca IBA ti s pht sinh r chui Tiu Hng.

Cng
thc th
nghim
C
CT1
CT2
CT3
CT4
CT5
CV%
LSD
T l
cy ra
r (%)
IBA
(mg/l)
0,0
0,2
0,4
0,6
0,8
1,0
62,59
77,41
82,59
90,00
95,19
83,33
0,7
1,07
S r/cy
Chiu di
r (cm)
Sau 3 tun
4,82
4,95
5,07
5,13
5,64
5,04
0,8
0,73
4,91
5,03
5,22
5,26
5,31
5,05
0,8
0,72
Mu sc r
Mu sc l
Xm
en
Trng
Xm
Trng Xm
Xm en
Xanh BT
Xanh BT
Xanh BT
Xanh BT
Xanh BT
Xanh nht
Hnh 3.3. nh hng ca cc cht kch thch sinh trng thuc nhm auxin
ti s pht sinh r ca chui Tiu Hng.
A. M chui sau 3 tun (C)
C. M chui sau 3 tun (IAA 0,6mg/l)
B. M chui sau 3 tun (-NAA 0,4mg/l)

D. M chui sau 3 tun (IBA 0,8mg/l)
35
3.4. nh hng ca t hp cc cht kch thch sinh trng thuc nhm

cytokinin v auxin n s sinh trng ca chui Tiu Hng.
3.4.1. nh hng ca t hp cht kch thch sinh trng BAP v -NAA
Cy con c nui cy trn mi trng MS c b sung saccharose
30g/l + agar 6,5g/l + nc da 100ml/l v t hp cc cht kch thch sinh
trng BAP nng 5mg/l, -NAA nng t 0,2mg/l - 0,8mg/l. Kt qu
c nh gi thng qua bng 3.7.
cc mc phi hp nng , mu nui cy u cho t l m sng cao.
Sau 3 tun theo di, t l m to chi mc phi hp nng BAP 5mg/l v
NAA 0,4mg/l cao nht t 59,15%. Cc ch tiu khc nh s chi/m, s
l/chi, chiu di trung bnh ca l gia cc cng thc th nghim khng c s
sai khc ng k.
Tuy nhin, cng tng t khi nghin cu v nh hng ca t hp BAP
v NAA ti s sinh trng ca chui Tiu hng, tng nng NAA s km
hm s sinh trng ca chui Tiu hng. C th, mc nng NAA
0,8mg/l, sau 3 tun theo di, t l m to chi t 47,41%, s chi/m t
3,68, s l/chi t 3,09, chiu di trung bnh ca l t 3,66cm. Cc ch tiu
trn u gim so vi mc nng NAA 0,4mg/l.
Nh vy, mi trng MS c bn + saccarose 30g/l + agar 6,5g/l +
nc da 100ml/l, b sung t hp cht kch thch sinh trng BAP 5mg/l
v NAA 0,4mg/l l mi trng thch hp cho s sinh trng ca cy chui
Tiu Hng.
36
Bng 3.7. nh hng ca t hp cht kch thch sinh trng BAP v NAA ti s sinh trng ca chui Tiu Hng.
Cng thc
T l
th nghim
m to
to
NAA
chi
chi/m
protocorm
(mg/l) (mg/l)
(%)
BAP
T l m
Chiu di
l/chi
Mu sc l
(cm)
(%)
Sau 3 tun
38,52
2,51
32,96
3,93
3,64
Xanh nht
5,0
0,2
49,63
3,76
56,67
4,01
4,87
Xanh BT
5,0
0,4
59,15
3,89
64,07
4,35
4,95
Xanh BT
5,0
0,6
43,33
3,67
46,30
3,97
4,73
Xanh BT
5,0
0,8
47,41
3,68
52,22
3,09
3,66
Xanh BT
CV%
0,8
2,6
1,3
0,8
0,8
LSD
0,67
1,39
1,3
0,59
0,62
3.4.2. nh hng ca t hp cht kch thch sinh trng BAP v IAA n

Nghin cu nh hng ca t hp cht kch thch sinh trng BAP
nng 5mg/l v IAA nng t 0,2mg/l - 0,8mg/l trn nn mi trng MS
c b sung saccarose 30g/l + agar 6,5g/l + nc da 100ml/l .
Kt qu cho thy, sau 3 tun theo di, mc phi hp nng BAP
5mg/l v IAA 0,6mg/l cho kt qu cao nht, t l m to chi t 59,26%, t
l m to protocorm t 55,93%. Cc ch tiu khc nh s chi/m, s
l/chi, chiu di trung bnh ca l gia cc cng thc th nghim khng c s
sai khc ng k.
Tuy nhin, khi tng nng IAA ln 0,8mg/l, tt c cc ch tiu nghin
cu u gim so vi mc nng IAA 0,4mg/l v 0,6mg/l. C th, mc
nng IAA 0,8mg/l, sau 3 tun theo di, t l m to chi t 46,30%, t l
m to cm chi t 47,03%, s chi/m t 3,57, s l/chi t 3,49, chiu
37
di trung bnh ca l t 3,76cm. Kt qu ny cho thy, IAA c tc dng kch

thch s sinh trng ca chui Tiu hng mc nng thch hp.
Bng 3.8. nh hng ca t hp cht kch thch sinh trng BAP v IAA
Cng thc
th nghim
BAP IAA
(mg/l) (mg/l)
T l
m
to
chi
(%)
S
chi/m
T l m
to cm
chi
(%)
S
l/chi
Chiu di
l
Mu sc l
(cm)
Sau 3 tun
0
34,07
1,43
28,52
3,42
3,57
5,0
0,2
45,93
3,66
45,93
3,56
3,74
5,0
5,0
0,4
0,6
51,85
59,26
3,59
3,74
48,89
55,93
3,65
3,77
4,85
5,90
5,0
0,8
46,30
3,57
47,03
3,49
3,76
3,0
2,6
2,0
0,11
0,6
0,48
2,9
0,19
2,3
0,18
CV%
LSD
Xanh
nht
Xanh
nht
Xanh BT
Xanh BT
Xanh
nht
Nh vy, mi trng MS c bn + saccarose 30g/l + agar 8,0g/l + nc

da 100ml/l, b sung t hp cht kch thch sinh trng BAP 5mg/l v IAA
0,6mg/l l thch hp cho s sinh trng ca chui Tiu hng.
3.4.3. nh hng ca t hp cht kch thch sinh trng BAP v IBA n
Tip tc nghin cu nh hng t hp cht kch thch sinh trng BAP
nng 5mg/l v IBA nng t 0,2mg/l n 0,8mg/l trn nn mi trng
MS c b sung saccarose 30g/l + agar 6,5g/l + nc da 100ml/l, kt qu thu
c th hin bng 3.9.
Kt qu cho thy, tt c cc cng thc, cy con u c t l m to
protocorm cao, cao nht l t hp BAP 5mg/l v IBA 0,8mg/l c t l m to
chi t 54,07%; s l/chi t 3,79; chiu di trung bnh ca l t 4,96cm
sau 3 tun nui cy. Nh vy, mi trng MS, b sung saccarose 30g/l + agar
38
6,5g/l + nc da 100ml/l v t hp BAP 5mg/l + IBA 0,8mg/l l mi trng

thch hp cho s sinh trng ca chui Tiu Hng.
Bng 3.9. nh hng ca t hp cht kch thch sinh trng BAP v IBA
Cng thc
T l
th nghim
m to
chi
BAP
IBA
(%)
(mg/l) (mg/l)
0
5,0
5,0
5,0
5,0
CV%
LSD
0
0,2
0,4
0,6
0,8
32,22
43,33
44,81
45,19
51,48
1,1
0,89
S
chi/m
1,41
3,56
3,59
3,67
3,68
3,1
1,18
T l m
Chiu di
to cm S l/chi
l
Mu sc l
chi
(cm)
(%)
Sau 3 tun
31,85
3,43
3,55
Xanh nht
47,78
3,51
3,76
Xanh nht
48,15
3,55
3,75
Xanh BT
50,37
3,67
4,70
Xanh BT
54,07
3,79
4,96
Xanh BT
1,3
2,0
1,2
1,09
0,13
0,87
Sau khi nghin cu nh hng t hp cc cht kch thch sinh trng:

BAP + -NAA, BAP + IAA, BAP + IBA, chng ti tip tc tin hnh so snh
ba t hp cht kch thch ny sau 3 tun nui cy tm ra nng thch hp
nht cho s sinh trng ca chui Tiu Hng.
Qua s liu bng 3.7, 3.8, 3.9, chng ti kt lun: mi trng MS c
bn + saccharose 30g/l + agar 6,5g/l + nc da 100ml/l, b sung t hp cht
kch thch sinh trng BAP 5mg/l v NAA 0,4mg/l l mi trng thch hp
nht cho s sinh trng ca chui Tiu Hng.
3.4.4. nh hng ca t hp cht kch thch sinh trng kinetin v -NAA
Nghin cu nh hng ca t hp cht kch thch sinh trng kinetin
v -NAA n s sinh trng ca chui Tiu Hng mc phi hp kinetin
nng 7,0 mg/l v -NAA nng t 0,2mg/l n 0,8mg/l. Kt qu thu
c th hin trong bng 3.10.
tt c cc cng thc c s kt hp ca kinetin v -NAA, t l m
39
to chi, t l m to protocorm, s chi/m, s l/chi v chiu di trung

bnh ca chi u cao hn hn so vi i chng. C th, sau 3 tun nui cy,
mi trng i chng khng b sung kinetin v -NAA, t l m to chi,
t l m to protocorm ln lt t 35,56%; 27,41%. Cc t l trn 2 mi
trng c b sung kinetin 7,0mg/l + -NAA 0,2mg/l, kinetin 7,0mg/l + NAA 0,4mg/l ln lt l 54,07%%; 49,23% v 58,15%; 60,37%. Tuy nhin,
khi c nh nng kinetin 7,0mg/l v tng dn nng -NAA ln
0,6mg/l v 0,8mg/l th cc ch tiu theo di u c xu hng gim xung.
So snh gia cc thang nng , cho thy b sung kinetin 7,0mg/l, NAA 0,4mg/l trn nn mi trng MS, b sung saccarose 30g/l + agar 6,5g/l
+ nc da 100ml/l l thch hp cho s sinh trng ca chui Tiu Hng.
Bng 3.10. nh hng ca t hp cht kch thch sinh trng kinetin v
-NAA ti s sinh trng ca chui Tiu Hng.
Cng thc
T l
th nghim
m
to
Kinetin NAA
(mg/l) (mg/l)
chi
T l m
S
to cm
chi/m
chi
S l/chi
Mu sc l
(cm)
(%)
(%)
Chiu di
Sau 3 tun
0
35,56
1,40
27,41
3,22
3,79
Xanh nht
7,0
0,2
54,07
3,57
49,23
4,39
4,81
Xanh BT
7,0
0,4
58,15
3,79
60,37
4,65
4,85
Xanh BT
7,0
0,6
52,96
3,71
53,70
4,54
4,78
Xanh BT
7,0
0,8
45,19
3,56
46,30
4,45
3,79
Xanh BT
CV%
0,8
1,9
3,0
5,7
3,0
LSD
0,76
0,11
0,23
0,4
0,23
40
3.4.5. nh hng ca t hp cht kch thch sinh trng kinetin v IAA

Nghin cu nh hng t hp cht kch thch sinh trng kinetin nng
7,0mg/l v IAA nng t 0,2mg/l n 0,8mg/l trn nn mi trng MS
c b sung saccarose 30g/l + agar 6,5g/l + nc da 100ml/l, kt qu thu
c th hin bng 3.11.
Sau 3 tun theo di, t l m to chi t 51,11%; t l m to
protocorm t 57,41%. Cc ch tiu khc nh s chi/m, s l/chi, chiu di
trung bnh ca l gia cc cng thc th nghim khng c s sai khc ng k.
Tuy nhin, khi tng nng IAA ln 0,6mg/l v 0,8mg/l, cc ch tiu
theo di u c hin tng gim xung. C th, mc nng IAA 0,8mg/l,
sau 3 tun theo di, t l m to chi t 48,15%, t l m to protocorm t
48,52%, s chi/m t 3,64, s l/chi t 3,99, chiu di trung bnh ca l
t3,76cm. Cc ch tiu trn u gim so vi mc nng IAA 0,2mg/l.
Qua nghin cu v quan st kt qu, nhn thy t hp kinetin 7,0mg/l v
IAA 0,2mg/l l nng thch hp cho s sinh trng ca cy chui Tiu Hng.
IAA ti s sinh trng ca chui Tiu Hng.
Cng thc
th nghim
Kinetin
(mg/l)
T l
m to
chi
IAA
(mg/l) (%)
S
chi/m
T l m
Chiu di
S l/chi
to
l
protocorm
(cm)
(%)
Mu sc l
Sau 3 tun
0
35,56
1,48
28,51
3,83
3,63
Xanh nht
7,0
0,2
51,11
3,78
57,41
4,11
5,90
Xanh BT
7,0
0,4
47,78
3,69
49,63
3,95
4,85
Xanh BT
7,0
0,6
48,89
3,64
45,56
3,97
3,75
Xanh BT
7,0
0,8
48,15
3,64
48,52
3,99
3,76
Xanh BT
CV%
1,6
1,1
1,2
0,8
2,2
LSD
1,33
0,67
0,97
0,59
0,17
41
3.4.6. nh hng ca t hp cht kch thch sinh trng kinetin v IBA

Nghin cu nh hng t hp cht kch thch sinh trng kinetin nng
7,0mg/l v IBA nng t 0,2mg/l n 0,8mg/l trn nn mi trng MS
c b sung saccarose 30g/l + agar 6,5g/l + nc da 100ml/l, kt qu thu
c th hin bng 3.12.
Sau 3 tun theo di, mc nng phi hp nng kinetin 7,0mg/l
v IBA 0,4mg/l cao nht nh t l m to chi t 52,59%; t l m to
protocorm t 59,26%. Cc ch tiu khc nh s chi/m, s l/chi, chiu di
trung bnh ca l gia cc cng thc th nghim khng c s sai khc ng k.
Tuy nhin, khi tng nng IBA ln 0,6mg/l v 0,8mg/l, cc ch tiu
theo di u c xu hng gim xung so vi mc nng IBA 0,4mg/l. C
th, mc nng IBA 0,8mg/l, sau 3 tun theo di, t l m to chi t
43,33%, t l m to protocorm t 49,26%, s chi/m t 3,58, s l/chi
t 3,99, chiu di trung bnh ca l t 4,76cm. Cc ch tiu trn u gim so
vi mc nng IBA 0,4mg/l.
Qua nghin cu v quan st kt qu, ti nhn thy t hp kinetin
7,0mg/l v IBA 0,4mg/l l nng thch hp cho s sinh trng ca cy
chui Tiu Hng.
42

IBA ti s sinh trng ca chui Tiu Hng.
Cng thc
T l
th nghim
Kinetin
(mg/l)
IBA
(mg/l)
to
chi
T l m
S
to
chi/m
protocorm
S l/chi
Chiu di
Mu sc
l
(cm)
(%)
(%)
Sau 3 tun
Xanh
34,07
1,43
29,26
3,45
3,60
7,0
0,2
42,22
3,77
47,41
4,01
3,78
Xanh BT
7,0
0,4
52,59
3,82
59,26
4,19
4,98
Xanh BT
7,0
0,6
40,37
3,61
45,19
3,95
4,67
Xanh BT
7,0
0,8
43,33
3,58
49,26
3,99
4,76
Xanh BT
CV%
2,4
2,6
1,0
1,9
1,5
LSD
1,81
0,15
0,83
0,13
0,12
nht
Sau khi nghin cu nh hng t hp cc cht kch thch sinh trng:

kinetin + -NAA, kinetin + IAA, kinetin + IBA, chng ti tip tc tin hnh
so snh ba t hp cht kch thch ny tm ra nng thch hp nht cho s
sinh trng ca chui Tiu Hng. Qua s liu bng 3.10, 3.11, 3.12, chng ti
kt lun: t hp kinetin 7,0mg/l v -NAA 0,4mg/l l nng thch hp nht
ca chui Tiu Hng.
Tip tc so snh gia bn mi trng nhn nhanh c b sung: BAP 5,0
mg/l; kientin 7,0mg/l; BAP 5mg/l + -NAA 0,4mg/l v kinetin 7,0mg/l + NAA 0,4mg/l th cng thc phi hp BAP 5mg/l + -NAA 0,4mg/l l nng
thch hp nht cho s sinh trng ca chui Tiu Hng.
43
3.5. nh hng ca gi th n s sinh trng ca chui Tiu Hng.

a cy ra ngoi vn m l giai on quan trng bao gm vic to
r, hun luyn thch nghi vi thay i ca nhit , m, s mt nc, su
bnh v chuyn t trng thi d dng sang t dng hon ton. y l giai
on quyt nh kh nng ng dng quy trnh nhn ging in vitro. Tu theo
iu kin nghin cu c th dng cc loi gi th khc nhau ra cy nh:
t, ct, tru hun Theo di s pht trin ca cy chui trong giai on u
ngoi mi trng nui cy, ti tin hnh trng 30 cy con trn cc gi th t,
ct, tru hun, t + tru hun, ct + tru hun, t + ct + tru hun. Kt qu thu
c th hin trong bng 3.13.
Bng 3.13. Kt qu ra cy
Sau 1 tun
T l
Sau 2 tun
T l
Sau 3 tun
T l
cy
cy
ra
cht
30
6,66
10,00
10,00
Ct
30
10,00
10,00
13,33
Tru hun
30
20,00
10
33,33
14
46,67
t + tru hun (1:1)
30
3,3
6,66
6,66
Ct + tru hun(1:1)
30
13,33
6,66
6,66
30
3,33
3,33
3,33
Loi gi th
t +Ct + tru hun

(1:1:1)
cy
cht
(%)
S
cy
cht
cy
cht
(%)
S
cy
cht
cy
cht
(%)
Kt qu bng trn cho thy sau 3 tun u theo di, d c chm

sc cn thn, gi m v che nng nhng do cha thch nghi c vi mi
trng bn ngoi nn cy thng b ho l v ng r dn n t l cy cht
kh cao. Cy chui trng trn gi th, tru hun c t l cy cht n 46,67,%
44
tng ng vi 14 cy con b cht. iu ny cho thy, gi th tru hun khng

ph hp trng chui Tiu Hng. Trong khi , vi gi th t + ct + tru
hun (1:1:1), t l cy cht 3,33%, thp nht trong cc loi gi th.
Trn thc t, cy con trng trn gi th tru hun thng cht do thi r.
tru hun c kh nng ht nc mnh, d gy ng. Gi th t + ct + tru hun
(1:1:1) l thch hp nht a cy chui Tiu Hng t trong ng nghim ra
mi trng t nhin.
Hnh 3.4. Hnh nh sinh trng ca chui Tiu Hng trn gi th

t + ct + tru hun (1:1:1)
45
KT LUN V KIN NGH

KT LUN
Trong iu kin ca Trung tm thng tin v ng dng tin b khoa hc
cng ngh tnh (Phng th nghim mi, ha cht thit b, i ng k thut p
ng...) th chng ti bc u tin hnh ti Nghin cu nhn nhanh in
vitro cy chui Tiu Hng ch ng ngun ging p ng nhu cu t bi
sn xut nng cho a phng.
Do vy, vi iu kin thc t ca c quan chng ti rt ra mt s kt lun sau:
1. Cng b sung saccarose 30g/l + agar 6,5g/l + nc da 100ml/l, mi
trng MS cho hiu qu nhn chi v to cm tt hn mi trng Phytamax
v mi trng B5.
2. Mi trng MS + saccarose 30g/l + agar 6,5g/l + nc da 100ml/l +
kinetin 7,0mg/l l mi trng tt nht cho s pht sinh chi v to cm chi
ca chui Tiu Hng. Tuy nhin, mi trng MS + saccarose 30g/l + agar
6,5g/l +nc da 100ml/l + BAP 5mg/l li l mi trng pht sinh chi v to
cm chi tt v mang li hiu qu kinh t cao hn.
3. Mi trng thch hp nht cho s pht sinh r ca chui Tiu Hng l
MS + saccarose 30g/l + agar 6,5g/l + nc da 100ml/l + 0,8mg/l IBA.
4. Cng thc phi hp BAP 5mg/l v -NAA 0,4mg/l trn nn mi
trng MS c bn, MS + saccarose 30g/l + agar 6,5g/l + nc da 100ml/l l
thch hp nht cho s sinh trng ca chui Tiu Hng.
5. Gi th tt nht cho s sinh trng ca chui t + ct + tru hun
(1:1:1).
KIN NGH
Do thi gian nghin cu c hn, chng ti mi ch thm d c mi
trng nhn nhanh v gi th ra cy chui Tiu Hng. Tuy nhin, trong nui cy
m v t bo thc vt l cung cp c ngun cy ging p ng nhu cu sn
xut. ngh tip tc nghin cu hon thin v ch trng (bn phn,
46
lng nc ti, su bnh hi) cho n giai on tr bung v thu hoch.

- Tip tc kho st s sinh trng v pht tin ca chui Tiu Hng
trong iu kin ca a phng.
47
TI LIU THAM KHO

I, Ting Vit
1. B Nng nghip & PTNT (2007), Ph duyt quy hoch pht trin rau qu
v hoa cy cnh n nm 2010, tm nhn 2020.
2. L Trn Bnh, H Hu Nh, L Th Mui (1997), Cng ngh sinh hc thc
vt trong ci tin ging cy trng, Nxb Nng nghip H Ni.
3. Ng Xun Bnh, Bi Bo Hon, Nguyn Thy H (2003), Gio trnh cng
ngh sinh hc, Nxb Nng nghip H Ni.
4. Champion J.(1976), Cy chui (ti liu dch ), Nxb Nng nghip, H Ni.
5. V Vn Chi, Dng c Tiu (1978), Phn loi thc vt bc cao. Nxb
H &THCN, H Ni.
6. ng Hng Dt (2000), Ngh lm vn cy n qu ba min, Nxb Vn
ha dn tc, H Ni.
7. Phm Vn Du (2005), Gio trnh k thut trng cy n qu. Nxb H Ni.
8. Nguyn Th Hun (2000), Gio trnh cy n qu, Nxb Nng Nghip.
9. o Duy Hng, L Th Ho, Nguyn Vn Hng, Ng Xun Bnh, Nghin
cu ti sinh chi ging cy chui ty ( Musa Paradisiaca) Bc Kn bng
phng php nui cy m, tp ch Nng nghip v pht trin nng thn,
thng 3/2012,( tr 11- 16).
10. Nguyn Hong Lc (2007), Nhp mn cng ngh sinh hc, Nxb i hc
Hu.
11. Tt Li (1981), Cy thuc v v thuc Vit Nam, Nxb Khoa hc k
thut, H Ni.
12. ng Th Mai (2001), Nghin cu xy dng quy trnh nhn ging mt s
ging chui Bt bng phng php nui cy m t bo thc vt, lun vn
tt nghip i hc, trng i hc Nng nghip I H Ni.
13.Trn Vn Minh (1997), Cng ngh t bo thc vt, trng i hc quc
gia TP.HCM, Nxb HQG TP.HCM.
14. Th Ngc Oanh, Hong Vn Ph, Nguyn Th Hng, Hong Th Bch
Tho(2004), Phng php th nghim ng rung, Nxb nng nghip, H
48
Ni.
15. V Ngc Phng, Nhn ging in vitro cy chui (cavendish sp) trn quy
m cng nghip, bo co khoa hc hi ngh cng ngh sinh hc ton quc
2009. Cng ngh sinh hc phc v nng-lm nghip, y dc v bo v
mi trng, ( tr 319-322).
16. Hong Minh Tn, Nguyn Quang Thch, V Quang Sng (2006), Gio
trnh sinh l hc thc vt, Nxb Nng nghip H Ni.
17. Nguyn Quang Thch, (1995), Cng ngh sinh hc thc vt, Nxb Nng
nghip, H Ni
18. Nguyn Quang Thch, Nguyn Th L Anh, Nguyn Th Phng Tho
(2005), Gio trnh cng ngh sinh hc nng nghip, Nxb Nng nghip H Ni.
19. Nguyn c Thnh (2000), Nui cy m t bo thc vt nghin cu v
ng dng, Nxb Nng nghip H Ni.
20. Chu Th Thm,Phan Th Li, Nguyn Vn T (2007), K thut trng
chui nng sut cao, Nxb H Ni.
21. on Th i Thuyn, Nguyn Th Qunh, Trn Vn Minh, Nguyn c
Minh Hng, Nguyn Vn Uyn(1993), Nhn ging chui bng phng
php nui cy m, Nui cy m Thc vt phc v cng tc ging cy
trng, Nxb Nng nghip, TP H Ch Minh.
22. Trn Th Tc, Cao Anh Long, Phm Vn Cn, Hong Ngc Thun, on
Th L (1998), Gio trnh cy n qu, Nxb Nng nghip, H Ni.
23. Nguyn Vn Uyn v cng s (1993), Nui cy m thc vt phc v cng
tc ging cy trng, Nxb Nng nghip H Ni.
24. Nguyn Vn Uyn (1995), Nhng phng php cng ngh sinh hc thc
vt, Tp 2, Nxb nng nghip TP. H Ch Minh.
25. Vin kinh t nng nghip (2005), Bo co tng quan cc nghin cu v
ngnh rau qu Vit Nam.
26. Nng Vnh (1995), Cng ngh t bo thc vt ng dng, Nxb Nng
Nghip H Ni.
27. Chu Vn Mn (2000), ng dng tin hc trong sinh hc, Nxb i hc
Quc gia H Ni.
28. Trn Duy Qu (1999), Cc phng php mi trong chn ging cy trng,
Nxb Nng nghip.
49
29. Nguyn Vn Song (2011), Nhn nhanh in vitro lan Kim ip

(Dendrobium chrysotoxum) - mt loi lan rng c nguy c tuyt chng,
Tp ch khoa hc i hc Hu, s 64, tr.127-136.
30. Nguyn Quang Thch v cng s (2003), Nghin cu xy dng quy trnh
nhn ging v nui trng phong lan Phalaenopsis (lan H ip), Bo co
khoa hc ton quc, Nxb Khoa hc v K thut, tr.850.
II, Ting Anh
31. Morton, J. (1987) Banana. In: Fruits of warm climates. Julia F. Morton,
Miami, FL.( p. 2946).
32. Murashige T. and Skoog F. (1962), A resied medium for rapid growth
and bioassays wirh tobacco tissue cultures, Physiol. Plant.
33. Street (1973), Plant tissue and cell culture, Bor monogrvol. II Black Well
Scient. Publ, London.
III, Trang Web
34. http:// faostat, fao, org/ site/ 340/ default, aspx
35. (http://kienthuctamnong.gov.vn)
50
PH LC
Ph lc 01: Bng thnh phn c bn mi trng nhn ging.
Bng 2.1. Thnh phn c bn ca mi trng MS
STT
Thnh phn
Nng (mg/l)
MS1
1
CaCl2
440
MS2
2
KH2PO4
170
KNO3
1900
MgSO4.7H2O
370
NH4NO3
1650
MS3
6
H3BO4
6,2
KI
0,83
MnSO4.4H2O
22,3
ZnSO4.7H2O
8,6
10
CoCl2.5H2O
0,025
11
CuSO4.5H2O
0,025
12
Na2MoO4.2H2O
0,25
MS4
13
FeSO4.7H2O
27,8
14
Na2EDTA
37,3
MS5
15
Glicine
16
Thiamine HCl
0,1
17
Pyridoccine HCl
0,5
18
Nicotinic axit
0,5
19
Myo-inositol
100
(Ngun: L Trn Bnh, 1997) [2].
51
Bng 2.2. Thnh phn c bn ca mi trng Phytamax

STT
Thnh phn
Nng (mg/l)
Peptone
2000
Mes
1000
P1
CaCl2
166
P2
KH2PO4
85,5
KNO3
950
MgSO4.7H2O
90,35
NH4NO3
825
P3
8
H3BO4
31
KI
0,415
10
MnSO4.4H2O
8,45
11
ZnSO4.7H2O
5,3
12
CoCl2.5H2O
0,0125
13
CuSO4.5H2O
0,0125
14
Na2MoO4.2H2O
0,125
P4
15
FeSO4.7H2O
27,8
16
Na2EDTA
37,26
P5
17
Thiamine HCl
0,1
18
Pyridoccine HCl
0,5
19
Nicotinic axit
0,5
20
Myo-inositol
100
(Ngun: L Trn Bnh, 1997) [2].
52
Bng 2.3. Thnh phn c bn ca mi trng Gamborgcs, 1976 (B5)

STT
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
Thnh phn
Peptone
Mes
P1
CaCl2
P2
Na2PO4 .H2O
KNO3
MgSO4.7H2O
(NH4)2SO4
P3
H3BO3
KI
MnSO4.H2O
ZnSO4.7H2O
CoCl2.6H2O
CuSO4.5H2O
Na2MoO4.2H2O
P4
FeSO4.7H2O
Na2EDTA
P5
Thiamine HCl
Pyridoccine HCl
Nicotinic axit
Myo-inositol
Nng (mg/l)
2000
1000
150
150
2,528
24,6
134
3,0
0,75
10
2,0
0,0025
0,0025
0,25
27,8
37,2
10
1,0
1,0
100
53
Ph lc 02: Kt qu x l s liu th nghim

Th nghim: nh hng ca BAP ti s pht sinh chi v to cm chi
chui Tiu Hng
BALANCED ANOVA FOR VARIATE
FILE D1
31/12/13 21:59
------------------------------------------------------------------ :PAGE
VARIATE V003 MS
LN
SOURCE OF VARIATION
DF
LN
SOURCE OF VARIATION
DF
LN
SOURCE OF VARIATION
DF
LN
SOURCE OF VARIATION
DF
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
5 937.884
187.577
****** 0.000 2
* RESIDUAL
12 .824700
.687250E-01
----------------------------------------------------------------------------* TOTAL (CORRECTED)
17 938.709
55.2182
----------------------------------------------------------------------------BALANCED ANOVA FOR VARIATE
MTC FILE D1
31/12/13 21:59
------------------------------------------------------------------ :PAGE
2
VARIATE V004 MTC
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
5 1288.78
257.757
****** 0.000 2
* RESIDUAL
12 .462892
.385743E-01
----------------------------------------------------------------------------* TOTAL (CORRECTED)
17 1289.25
75.8380
SC/M FILE D1
31/12/13 21:59
------------------------------------------------------------------ :PAGE
3
VARIATE V005 SC/M
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
5 15.6618
3.13236
266.20 0.000 2
* RESIDUAL
12 .141201
.117667E-01
----------------------------------------------------------------------------* TOTAL (CORRECTED)
17 15.8030
.929588
CCC FILE D1
31/12/13 21:59
------------------------------------------------------------------ :PAGE
4
VARIATE V006 CCC
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
5 4.33045
.866090
191.05 0.000 2
* RESIDUAL
12 .544006E-01 .453339E-02
----------------------------------------------------------------------------* TOTAL (CORRECTED)
17 4.38485
.257932
----------------------------------------------------------------------------- TABLE OF
MEANS FOR FACTORIAL EFFECTS FILE D1
31/12/13 21:59
------------------------------------------------------------------ :PAGE
5
MEANS FOR EFFECT CT
------------------------------------------------------------------------------CT
1
2
3
4
5
6
NOS
3
3
3
3
3
3
31.8500
40.0000
41.4800
58.8900
39.2600
36.3000
MTC
1.67000
3.62000
3.85000
4.58000
4.15000
4.03000
SC/M
3.08000
2.56000
2.72000
1.49000
2.59000
2.67000
CCC
SE(N=
3)
0.113394
0.626278E-01 0.388732E-01
5%LSD 12DF
0.349404
0.192978
0.119782
------------------------------------------------------------------------------ANALYSIS OF VARIANCE SUMMARY TABLE
FILE D1
31/12/13 21:59
54
------------------------------------------------------------------ :PAGE
F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL. SECTION - 1

VARIATE
MS
MTC
SC/M
CCC
GRAND MEAN
(N=
18)
NO.
OBS.
18 79.012
18 41.297
18 3.6500
18 2.5183
STANDARD
DEVIATION C OF V |CT
-------------------- SD/MEAN |
BASED ON
BASED ON
%
|
TOTAL SS
RESID SS
|
7.4309
0.26215
0.3 0.0000
8.7085
0.19640
0.5 0.0000
0.96415
0.10847
3.0 0.0000
0.50787
0.67330E-01 2.7 0.0000
|
|
|
|
Th nghim: nh hng ca kinetin ti s pht sinh chi v to cm chi

chui Tiu Hng
TLM FILE D2
31/12/13 22:14
------------------------------------------------------------------ :PAGE
VARIATE V003 TLM
LN
SOURCE OF VARIATION
DF
LN
SOURCE OF VARIATION
DF
LN
SOURCE OF VARIATION
DF
LN
SOURCE OF VARIATION
DF
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
5 825.976
165.195
****** 0.000 2
* RESIDUAL
12 .104694
.872453E-02
----------------------------------------------------------------------------* TOTAL (CORRECTED)
17 826.081
48.5930
TLMTC FILE D2
31/12/13 22:14
------------------------------------------------------------------ :PAGE
2
VARIATE V004 TLMTC
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
5 2522.00
504.399
30.16 0.000 2
* RESIDUAL
12 200.677
16.7231
----------------------------------------------------------------------------* TOTAL (CORRECTED)
17 2722.67
160.157
SC/M FILE D2
31/12/13 22:14
------------------------------------------------------------------ :PAGE
3
VARIATE V005 SC/M
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
5 11.8632
2.37264
375.61 0.000 2
* RESIDUAL
12 .758003E-01 .631669E-02
----------------------------------------------------------------------------* TOTAL (CORRECTED)
17 11.9390
.702294
CCC FILE D2
31/12/13 22:14
------------------------------------------------------------------ :PAGE
4
VARIATE V006 CCC
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
5 10.2789
2.05577
555.62 0.000 2
* RESIDUAL
12 .443998E-01 .369998E-02
----------------------------------------------------------------------------* TOTAL (CORRECTED)
17 10.3233
.607250
----------------------------------------------------------------------------TABLE OF MEANS FOR FACTORIAL EFFECTS FILE D2
31/12/13 22:14
------------------------------------------------------------------ :PAGE
5
MEANS FOR EFFECT CT
------------------------------------------------------------------------------CT
1
NOS
3
TLMTC
31.4800
SC/M
1.72000
CCC
1.39000
55
2
3
4
5
6
3
3
3
3
3
46.1200
47.4100
52.5900
63.3300
67.4100
3.58000
3.83000
3.87000
4.13000
3.87000
1.46000
2.52000
2.47000
3.64000
2.45000
SE(N=
3)
2.36101
0.458864E-01 0.351187E-01
5%LSD 12DF
7.27508
0.141392
0.108213
------------------------------------------------------------------------------ANALYSIS OF VARIANCE SUMMARY TABLE FILE D2
31/12/13 22:14
------------------------------------------------------------------ :PAGE

VARIATE
TLMTC
SC/M
CCC
GRAND MEAN
(N=
18)
NO.
OBS.
18 51.390
18 3.5000
18 2.3217
STANDARD
-------------------- SD/MEAN |
BASED ON
BASED ON
%
|
TOTAL SS
RESID SS
|
12.655
4.0894
8.0 0.0000
0.83803
0.79478E-01 2.3 0.0000
0.77926
0.60827E-01 2.6 0.0000
|
|
|
|
Th nghim: nh hng ca NAA ti s pht sinh r chui Tiu Hng

TLCRR FILE D3
31/12/13 22:27
------------------------------------------------------------------ :PAGE
VARIATE V003 TLCRR
LN
SOURCE OF VARIATION
DF
LN
SOURCE OF VARIATION
DF
LN
SOURCE OF VARIATION
DF
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
5 1195.53
239.106
****** 0.000 2
* RESIDUAL
12 .576117E-01 .480097E-02
----------------------------------------------------------------------------* TOTAL (CORRECTED)
17 1195.59
70.3286
SR/C FILE D3
31/12/13 22:27
------------------------------------------------------------------ :PAGE
2
VARIATE V004 SR/C
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
5 4.32220
.864440
253.01 0.000 2
* RESIDUAL
12 .410002E-01 .341668E-02
----------------------------------------------------------------------------* TOTAL (CORRECTED)
17 4.36320
.256659
CD FILE D3
31/12/13 22:27
------------------------------------------------------------------ :PAGE
3
VARIATE V005 CD
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
5 1.71700
.343400
113.21 0.000 2
* RESIDUAL
12 .363997E-01 .303331E-02
----------------------------------------------------------------------------* TOTAL (CORRECTED)
17 1.75340
.103141
31/12/13 22:27
------------------------------------------------------------------ :PAGE
4
MEANS FOR EFFECT CT
------------------------------------------------------------------------------CT
1
2
3
4
5
6
NOS
3
3
3
3
3
3
TLCRR
71.1900
82.0900
91.4800
94.0700
75.1600
81.0700
SR/C
4.97000
6.06000
6.27000
6.48000
6.24000
6.14000
CD
4.99000
5.17000
5.94000
5.27000
5.19000
5.10000
56
SE(N=
3)
0.400041E-01 0.337475E-01 0.317978E-01
5%LSD 12DF
0.123266
0.103988
0.979800E-01
31/12/13 22:27
------------------------------------------------------------------ :PAGE

VARIATE
TLCRR
SR/C
CD
GRAND MEAN
(N=
18)
NO.
OBS.
18 82.510
18 6.0267
18 5.2767
STANDARD
-------------------- SD/MEAN |
BASED ON
BASED ON
%
|
TOTAL SS
RESID SS
|
8.3862
0.69289E-01 0.1 0.0000
0.50662
0.58452E-01 1.0 0.0000
0.32116
0.55075E-01 1.0 0.0000
|
|
|
|
Th nghim: nh hng ca IAA ti s pht sinh r chui Tiu Hng

TLCRR FILE D5
31/12/13 22:37
------------------------------------------------------------------ :PAGE
VARIATE V003 TLCRR
LN
SOURCE OF VARIATION
DF
LN
SOURCE OF VARIATION
DF
LN
SOURCE OF VARIATION
DF
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
5 1782.70
356.540
****** 0.000 2
* RESIDUAL
12 2.16734
.180612
----------------------------------------------------------------------------* TOTAL (CORRECTED)
17 1784.87
104.992
SR/C FILE D5
31/12/13 22:37
------------------------------------------------------------------ :PAGE
2
VARIATE V004 SR/C
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
5 3.31800
.663600
232.84 0.000 2
* RESIDUAL
12 .342002E-01 .285002E-02
----------------------------------------------------------------------------* TOTAL (CORRECTED)
17 3.35220
.197188
CDL FILE D5
31/12/13 22:37
------------------------------------------------------------------ :PAGE
3
VARIATE V005 CDL
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
5 .415000
.830000E-01 47.88 0.000 2
* RESIDUAL
12 .208000E-01 .173333E-02
----------------------------------------------------------------------------* TOTAL (CORRECTED)
17 .435800
.256353E-01
----------------------------------------------------------------------------ABLE OF MEANS FOR FACTORIAL EFFECTS FILE D5
31/12/13 22:37
------------------------------------------------------------------ :PAGE
4
MEANS FOR EFFECT CT
------------------------------------------------------------------------------CT
1
2
3
4
5
6
NOS
3
3
3
3
3
3
TLCRR
64.4400
72.9600
87.0400
95.5600
83.3300
82.2200
SR/C
4.61000
4.87000
4.92000
5.95000
4.93000
4.84000
CDL
5.22000
5.41000
5.45000
5.69000
5.28000
5.33000
SE(N=
3)
0.245365
0.308222E-01 0.240370E-01
5%LSD 12DF
0.756053
0.949736E-01 0.740662E-01
-------------------------------------------------------------------------------
57
ANALYSIS OF VARIANCE SUMMARY TABLE FILE D5

31/12/13 22:37
------------------------------------------------------------------ :PAGE

VARIATE
TLCRR
SR/C
CDL
GRAND MEAN
(N=
18)
NO.
OBS.
18 80.925
18 5.0200
18 5.3967
STANDARD
-------------------- SD/MEAN |
BASED ON
BASED ON
%
|
TOTAL SS
RESID SS
|
10.247
0.42498
0.5 0.0000
0.44406
0.53386E-01 1.1 0.0000
0.16011
0.41633E-01 0.8 0.0000
|
|
|
|
Th nghim: nh hng ca IBA ti s pht sinh r chui Tiu Hng

TLCRR FILE D6
1/ 1/14 0:49
------------------------------------------------------------------ :PAGE
VARIATE V003 TLCRR
LN
SOURCE OF VARIATION
DF
LN
SOURCE OF VARIATION
DF
LN
SOURCE OF VARIATION
DF
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
5 1905.25
381.051
****** 0.000 2
* RESIDUAL
12 4.39857
.366548
----------------------------------------------------------------------------* TOTAL (CORRECTED)
17 1909.65
112.333
SR/L FILE D6
1/ 1/14 0:49
------------------------------------------------------------------ :PAGE
2
VARIATE V004 SR/L
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
5 1.19245
.238490
138.93 0.000 2
* RESIDUAL
12 .205998E-01 .171665E-02
----------------------------------------------------------------------------* TOTAL (CORRECTED)
17 1.21305
.713559E-01
CDR FILE D6
1/ 1/14 0:49
------------------------------------------------------------------ :PAGE
3
VARIATE V005 CDR
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
5 .366600
.733200E-01 44.89 0.000 2
* RESIDUAL
12 .196000E-01 .163333E-02
----------------------------------------------------------------------------* TOTAL (CORRECTED)
17 .386200
.227176E-01
1/ 1/14 0:49
------------------------------------------------------------------ :PAGE
4
MEANS FOR EFFECT CT
------------------------------------------------------------------------------CT
1
2
3
4
5
6
NOS
3
3
3
3
3
3
TLCRR
62.6600
77.4100
82.5900
90.0000
95.1900
83.3300
SR/L
4.82000
4.95000
5.07000
5.13000
5.64000
5.04000
CDR
4.91000
5.03000
5.22000
5.26000
5.31000
5.05000
SE(N=
3)
0.349546
0.239211E-01 0.233333E-01
5%LSD 12DF
1.07707
0.737090E-01 0.718979E-01
1/ 1/14 0:49
------------------------------------------------------------------ :PAGE
58
VARIATE
TLCRR
SR/L
CDR
GRAND MEAN
(N=
18)
NO.
OBS.
18 81.863
18 5.1083
18 5.1300
STANDARD
-------------------- SD/MEAN |
BASED ON
BASED ON
%
|
TOTAL SS
RESID SS
|
10.599
0.60543
0.7 0.0000
0.26713
0.41433E-01 0.8 0.0000
0.15072
0.40415E-01 0.8 0.0000
|
|
|
|
Th nghim: nh hng ca t hp cht kch thch sinh trng BAP v NAA

ti s sinh trng chui Tiu Hng
TLMS FILE D7
1/ 1/14 1:28
------------------------------------------------------------------ :PAGE
VARIATE V003 TLMS
LN
SOURCE OF VARIATION
DF
LN
SOURCE OF VARIATION
DF
LN
SOURCE OF VARIATION
DF
LN
SOURCE OF VARIATION
DF
LN
SOURCE OF VARIATION
DF
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
4 1551.94
387.984
466.07 0.000 2
* RESIDUAL
10 8.32465
.832465
----------------------------------------------------------------------------* TOTAL (CORRECTED)
14 1560.26
111.447
-----------------------------------------------------------------------------BALANCED
ANOVA FOR VARIATE
TLMTC FILE D7
1/ 1/14 1:28
------------------------------------------------------------------ :PAGE
2
VARIATE V004 TLMTC
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
4 716.697
179.174
****** 0.000 2
* RESIDUAL
10 1.37550
.137550
----------------------------------------------------------------------------* TOTAL (CORRECTED)
14 718.073
51.2909
SC/M FILE D7
1/ 1/14 1:28
------------------------------------------------------------------ :PAGE
3
VARIATE V005 SC/M
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
4 7.25124
1.81281
3.11 0.006 2
* RESIDUAL
10 5.83060
.583060
----------------------------------------------------------------------------* TOTAL (CORRECTED)
14 13.0818
.934417
TLMTR FILE D7
1/ 1/14 1:28
------------------------------------------------------------------ :PAGE
4
VARIATE V006 TLMTR
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
4 501.541
125.385
244.92 0.000 2
* RESIDUAL
10 5.11949
.511949
----------------------------------------------------------------------------* TOTAL (CORRECTED)
14 506.660
36.1900
SL/C FILE D7
1/ 1/14 1:28
------------------------------------------------------------------ :PAGE
5
VARIATE V007 SL/C
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
4 2.61600
.654000
628.84 0.000 2
* RESIDUAL
10 .104000E-01 .104000E-02
----------------------------------------------------------------------------* TOTAL (CORRECTED)
14 2.62640
.187600
59

CDL FILE D7
1/ 1/14 1:28
------------------------------------------------------------------ :PAGE
6
VARIATE V008 CDL
LN
SOURCE OF VARIATION
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
4 5.25900
1.31475
****** 0.000 2
* RESIDUAL
10 .118003E-01 .118003E-02
----------------------------------------------------------------------------* TOTAL (CORRECTED)
14 5.27080
.376486
1/ 1/14 1:28
------------------------------------------------------------------ :PAGE
7
MEANS FOR EFFECT CT
------------------------------------------------------------------------------CT
1
2
3
4
5
SE(N=
5%LSD
NOS
3
3
3
3
3
3)
10DF
CT
1
2
3
4
5
NOS
3
3
3
3
3
DF
TLMTC
38.5200
49.6300
59.1500
43.2533
47.4100
SC/M
2.51000
3.76000
3.89000
3.67000
4.68000
TLMTR
52.6267
56.6700
63.8100
46.3000
52.2200
0.214126
0.674719
0.440855
1.38915
0.413098
1.30169
SL/C
3.93000
4.01000
4.35000
3.97000
3.09000
CDL
3.64000
4.87000
4.95000
4.73000
3.66000
SE(N=
3)
0.186190E-01 0.198329E-01
5%LSD 10DF
0.586692E-01 0.624942E-01
1/ 1/14 1:28
------------------------------------------------------------------ :PAGE

VARIATE
TLMTC
SC/M
TLMTR
SL/C
CDL
GRAND MEAN
(N=
15)
NO.
OBS.
15 47.593
15 3.7020
15 54.325
15 3.8700
15 4.3700
STANDARD
-------------------- SD/MEAN |
BASED ON
BASED ON
%
|
TOTAL SS
RESID SS
|
7.1618
0.37088
0.8 0.0000
0.96665
0.76358
2.6 0.0062
6.0158
0.71551
1.3 0.0000
0.43313
0.32249E-01 0.8 0.0000
0.61358
0.34352E-01 0.8 0.0000
|
|
|
|
Th nghim: nh hng ca t hp cht kch thch sinh trng BAP v IAA

ti s sinh trng chui Tiu Hng BALANCED ANOVA FOR VARIATE
TLMS FILE D8
31/12/13 23:30
------------------------------------------------------------------ :PAGE
VARIATE V003 TLMS
LN
SOURCE OF VARIATION
DF
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
4 1210.56
302.640
****** 0.000 2
* RESIDUAL
10 1.78060
.178060
----------------------------------------------------------------------------* TOTAL (CORRECTED)
14 1212.34
86.5957
TLMTC FILE D8
31/12/13 23:30
------------------------------------------------------------------ :PAGE
2
VARIATE V004 TLMTC
60
LN
SOURCE OF VARIATION
DF
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
4 897.012
224.253
109.35 0.000 2
* RESIDUAL
10 20.5072
2.05072
----------------------------------------------------------------------------* TOTAL (CORRECTED)
14 917.519
65.5371
SC/M FILE D8
31/12/13 23:30
------------------------------------------------------------------ :PAGE
3
VARIATE V005 SC/M
LN
SOURCE OF VARIATION
DF
LN
SOURCE OF VARIATION
DF
LN
SOURCE OF VARIATION
DF
LN
SOURCE OF VARIATION
DF
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
4 11.7752
2.94381
754.77 0.000 2
* RESIDUAL
10 .390027E-01 .390027E-02
----------------------------------------------------------------------------* TOTAL (CORRECTED)
14 11.8142
.843874
TLMTCC FILE D8
31/12/13 23:30
------------------------------------------------------------------ :PAGE
4
VARIATE V006 TLMTCC
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
4 1232.51
308.126
****** 0.000 2
* RESIDUAL
10 .700587
.700587E-01
----------------------------------------------------------------------------* TOTAL (CORRECTED)
14 1233.21
88.0861
SL/C FILE D8
31/12/13 23:30
------------------------------------------------------------------ :PAGE
5
VARIATE V007 SL/C
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
4 .225240
.563100E-01
5.09 0.017 2
* RESIDUAL
10 .110600
.110600E-01
----------------------------------------------------------------------------* TOTAL (CORRECTED)
14 .335840
.239886E-01
CDL FILE D8
31/12/13 23:30
------------------------------------------------------------------ :PAGE
6
VARIATE V008 CDL
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
4 11.9404
2.98509
292.08 0.000 2
* RESIDUAL
10 .102200
.102200E-01
----------------------------------------------------------------------------* TOTAL (CORRECTED)
14 12.0426
.860183
31/12/13 23:30
------------------------------------------------------------------ :PAGE
7
MEANS FOR EFFECT CT
------------------------------------------------------------------------------CT
1
2
3
4
5
SE(N=
5%LSD
3)
10DF
CT
1
2
NOS
3
3
3
3
3
TLMTC
35.7367
45.9300
51.8500
59.2600
46.3000
0.826786
2.60523
NOS
3
3
SL/C
3.42000
3.56000
SC/M
1.43000
3.66000
3.59000
3.74000
3.57000
TLMTCC
28.5200
45.9300
48.8900
55.9300
47.0300
0.360568E-01 0.152817
0.113616
0.481531
CDL
3.57000
3.74000
61
3
4
5
3
3
3
3.65000
3.77000
3.49000
4.85000
5.90000
3.76000
SE(N=
3)
0.607179E-01 0.583665E-01
5%LSD 10DF
0.191324
0.183915
31/12/13 23:30
------------------------------------------------------------------ :PAGE

VARIATE
TLMTC
SC/M
TLMTCC
SL/C
CDL
GRAND MEAN
(N=
15)
NO.
OBS.
15 47.815
15 3.1980
15 45.260
15 3.5780
15 4.3640
STANDARD
-------------------- SD/MEAN |
BASED ON
BASED ON
%
|
TOTAL SS
RESID SS
|
8.0955
1.4320
3.0 0.0000
0.91863
0.62452E-01 2.0 0.0000
9.3854
0.26469
0.6 0.0000
0.15488
0.10517
2.9 0.0171
0.92746
0.10109
2.3 0.0000
|
|
|
|
Th nghim: nh hng ca t hp cht kch thch sinh trng BAP v IBA

TLMS FILE D9
31/12/13 23:41
------------------------------------------------------------------ :PAGE
VARIATE V003 TLMS
LN
SOURCE OF VARIATION
DF
LN
SOURCE OF VARIATION
DF
LN
SOURCE OF VARIATION
DF
LN
SOURCE OF VARIATION
DF
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
4 1067.30
266.825
****** 0.000 2
* RESIDUAL
10 .657417
.657417E-01
----------------------------------------------------------------------------* TOTAL (CORRECTED)
14 1067.96
76.2828
TLMTC FILE D9
31/12/13 23:41
------------------------------------------------------------------ :PAGE
2
VARIATE V004 TLMTC
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
4 582.518
145.630
595.26 0.000 2
* RESIDUAL
10 2.44650
.244650
----------------------------------------------------------------------------* TOTAL (CORRECTED)
14 584.965
41.7832
SC/M FILE D9
31/12/13 23:41
------------------------------------------------------------------ :PAGE
3
VARIATE V005 SC/M
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
4 11.8064
2.95161
308.10 0.000 2
* RESIDUAL
10 .958014E-01 .958014E-02
----------------------------------------------------------------------------* TOTAL (CORRECTED)
14 11.9022
.850160
TLTCC FILE D9
31/12/13 23:41
------------------------------------------------------------------ :PAGE
4
VARIATE V006 TLTCC
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
4 1162.53
290.632
812.54 0.000 2
* RESIDUAL
10 3.57685
.357685
-----------------------------------------------------------------------------
62
* TOTAL (CORRECTED)
14 1166.11
83.2932
SL/C FILE D9
31/12/13 23:41
------------------------------------------------------------------ :PAGE
5
VARIATE V007 SL/C
LN
SOURCE OF VARIATION
DF
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
4 .240000
.600000E-01 12.05 0.001 2
* RESIDUAL
10 .497999E-01 .497999E-02
----------------------------------------------------------------------------* TOTAL (CORRECTED)
14 .289800
.207000E-01
CDL FILE D9
31/12/13 23:41
------------------------------------------------------------------ :PAGE
6
VARIATE V008 CDL
LN
SOURCE OF VARIATION
DF
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
4 4.89156
1.22289
536.35 0.000 2
* RESIDUAL
10 .228001E-01 .228001E-02
----------------------------------------------------------------------------* TOTAL (CORRECTED)
14 4.91436
.351026
31/12/13 23:41
------------------------------------------------------------------ :PAGE
7
MEANS FOR EFFECT CT
------------------------------------------------------------------------------CT
1
2
3
4
5
SE(N=
5%LSD
NOS
3
3
3
3
3
3)
10DF
CT
1
2
3
4
5
NOS
3
3
3
3
3
TLMTC
32.2200
43.2533
44.2600
45.1900
51.4800
SC/M
1.41000
3.56000
3.59000
3.67000
3.68000
TLTCC
31.8500
47.7800
48.1500
50.3700
59.0700
0.285570
0.899841
0.565100E-01 0.345295
0.178065
1.08804
SL/C
3.43000
3.51000
3.55000
3.67000
3.79000
CDL
3.55000
3.76000
3.75000
4.70000
4.96000
SE(N=
3)
0.407431E-01 0.275682E-01
5%LSD 10DF
0.128383
0.868683E-01
31/12/13 23:41
------------------------------------------------------------------ :PAGE

VARIATE
TLMTC
SC/M
TLTCC
SL/C
CDL
GRAND MEAN
(N=
15)
NO.
OBS.
15 43.281
15 3.1820
15 47.444
15 3.5900
15 4.1440
STANDARD
-------------------- SD/MEAN |
BASED ON
BASED ON
%
|
TOTAL SS
RESID SS
|
6.4640
0.49462
1.1 0.0000
0.92204
0.97878E-01 3.1 0.0000
9.1265
0.59807
1.3 0.0000
0.14387
0.70569E-01 2.0 0.0009
0.59247
0.47749E-01 1.2 0.0000
|
|
|
|
Th nghim: nh hng ca t hp cht kch thch sinh trng kinetin v

NAA ti s sinh trng chui Tiu Hng
TLMS FILE D10
31/12/13 23:47
------------------------------------------------------------------ :PAGE
VARIATE V003 TLMS
63
LN
SOURCE OF VARIATION
DF
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
4 2228.88
557.220
8.31 0.003 2
* RESIDUAL
10 670.628
67.0628
----------------------------------------------------------------------------* TOTAL (CORRECTED)
14 2899.51
207.108
TLMTC FILE D10
31/12/13 23:47
------------------------------------------------------------------ :PAGE
2
VARIATE V004 TLMTC
LN
SOURCE OF VARIATION
DF
LN
SOURCE OF VARIATION
DF
LN
SOURCE OF VARIATION
DF
LN
SOURCE OF VARIATION
DF
LN
SOURCE OF VARIATION
DF
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
4 960.257
240.064
****** 0.000 2
* RESIDUAL
10 1.74428
.174428
----------------------------------------------------------------------------* TOTAL (CORRECTED)
14 962.001
68.7144
SC/M FILE D10
31/12/13 23:47
------------------------------------------------------------------ :PAGE
3
VARIATE V005 SC/M
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
4 12.3946
3.09866
849.68 0.000 2
* RESIDUAL
10 .364685E-01 .364685E-02
----------------------------------------------------------------------------* TOTAL (CORRECTED)
14 12.4311
.887935
TLMTCC FILE D10
31/12/13 23:47
------------------------------------------------------------------ :PAGE
4
VARIATE V006 TLMTCC
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
4 3.77736
.944340
55.48 0.000 2
* RESIDUAL
10 .170200
.170200E-01
----------------------------------------------------------------------------* TOTAL (CORRECTED)
14 3.94756
.281969
----------------------------------------------------------------------------- BALANCED
ANOVA FOR VARIATE
SL/C FILE D10
31/12/13 23:47
------------------------------------------------------------------ :PAGE
5
VARIATE V007 SL/C
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
4 4.45380
1.11345
22.72 0.000 2
* RESIDUAL
10 .490000
.490000E-01
----------------------------------------------------------------------------* TOTAL (CORRECTED)
14 4.94380
.353129
CDL FILE D10
31/12/13 23:47
------------------------------------------------------------------ :PAGE
6
VARIATE V008 CDL
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
4 3.77736
.944340
55.48 0.000 2
* RESIDUAL
10 .170200
.170200E-01
----------------------------------------------------------------------------* TOTAL (CORRECTED)
14 3.94756
.281969
31/12/13 23:47
------------------------------------------------------------------ :PAGE
7
MEANS FOR EFFECT CT
-------------------------------------------------------------------------------
64
CT
1
2
3
4
5
SE(N=
5%LSD
NOS
3
3
3
3
3
3)
10DF
CT
1
2
3
4
5
NOS
3
3
3
3
3
TLMTC
35.5600
54.0700
58.1500
52.9600
45.1900
SC/M
1.40000
3.57000
3.79000
3.72667
3.56000
TLMTCC
3.79000
4.81000
4.85000
4.78000
3.79000
0.241128
0.759802
0.348657E-01 0.753215E-01
0.109863
0.237341
SL/C
3.22000
3.39000
3.65000
4.54000
4.45000
CDL
3.79000
4.81000
4.85000
4.78000
3.79000
SE(N=
3)
0.127802
0.753215E-01
5%LSD 10DF
0.402709
0.237341
31/12/13 23:47
------------------------------------------------------------------ :PAGE

VARIATE
TLMTC
SC/M
TLMTCC
SL/C
CDL
GRAND MEAN
(N=
15)
NO.
OBS.
15 49.186
15 3.2093
15 4.4040
15 3.8500
15 4.4040
STANDARD
-------------------- SD/MEAN |
BASED ON
BASED ON
%
|
TOTAL SS
RESID SS
|
8.2894
0.41765
0.8 0.0000
0.94230
0.60389E-01 1.9 0.0000
0.53101
0.13046
3.0 0.0000
0.59425
0.22136
5.7 0.0001
0.53101
0.13046
3.0 0.0000
|
|
|
|
Th nghim: nh hng ca t hp cht kch thch sinh trng kinetin v IAA

TLMS FILE D11
31/12/13 23:57
------------------------------------------------------------------ :PAGE
VARIATE V003 TLMS
LN
SOURCE OF VARIATION
DF
LN
SOURCE OF VARIATION
DF
LN
SOURCE OF VARIATION
DF
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
4 1035.60
258.899
501.94 0.000 2
* RESIDUAL
10 5.15792
.515792
----------------------------------------------------------------------------* TOTAL (CORRECTED)
14 1040.75
74.3395
TLMTC FILE D11
31/12/13 23:57
------------------------------------------------------------------ :PAGE
2
VARIATE V004 TLMTC
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
4 452.659
113.165
210.85 0.000 2
* RESIDUAL
10 5.36717
.536717
----------------------------------------------------------------------------* TOTAL (CORRECTED)
14 458.026
32.7162
SC/M FILE D11
31/12/13 23:57
------------------------------------------------------------------ :PAGE
3
VARIATE V005 SC/M
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
4 11.7346
2.93364
****** 0.000 2
* RESIDUAL
10 .136026E-01 .136026E-02
-----------------------------------------------------------------------------
65
* TOTAL (CORRECTED)
14 11.7482
.839154
TLMTP FILE D11
31/12/13 23:57
------------------------------------------------------------------ :PAGE
4
VARIATE V006 TLMTP
LN
SOURCE OF VARIATION
DF
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
4 1369.59
342.398
****** 0.000 2
* RESIDUAL
10 2.84825
.284825
----------------------------------------------------------------------------* TOTAL (CORRECTED)
14 1372.44
98.0314
SL/C FILE D11
31/12/13 23:57
------------------------------------------------------------------ :PAGE
5
VARIATE V007 SL/C
LN
SOURCE OF VARIATION
DF
LN
SOURCE OF VARIATION
DF
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
4 .120000
.300000E-01 28.30 0.000 2
* RESIDUAL
10 .106000E-01 .106000E-02
----------------------------------------------------------------------------* TOTAL (CORRECTED)
14 .130600
.932858E-02
CDL FILE D11
31/12/13 23:57
------------------------------------------------------------------ :PAGE
6
VARIATE V008 CDL
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
4 11.6252
2.90631
323.64 0.000 2
* RESIDUAL
10 .898003E-01 .898003E-02
----------------------------------------------------------------------------* TOTAL (CORRECTED)
14 11.7150
.836789
31/12/13 23:57
------------------------------------------------------------------ :PAGE
7
MEANS FOR EFFECT CT
------------------------------------------------------------------------------CT
1
2
3
4
5
SE(N=
5%LSD
NOS
3
3
3
3
3
3)
10DF
CT
1
2
3
4
5
TLMTC
35.5600
51.1133
47.7967
48.8900
48.1500
0.422972
1.33280
NOS
3
3
3
3
3
SL/C
3.83000
4.11000
3.95000
3.97000
3.99000
SC/M
1.48000
3.78000
3.69000
3.64000
3.64000
TLMTP
28.5100
57.4100
49.7300
45.5600
48.5200
0.212937E-01 0.308126
0.670971E-01 0.970917
CDL
3.63000
5.90000
4.85000
3.75000
3.76000
SE(N=
3)
0.187972E-01 0.547115E-01
5%LSD 10DF
0.592306E-01 0.172398
31/12/13 23:57
------------------------------------------------------------------ :PAGE

VARIATE
TLMTC
GRAND MEAN
(N=
15)
NO.
OBS.
15 46.302
STANDARD
-------------------- SD/MEAN |
BASED ON
BASED ON
%
|
TOTAL SS
RESID SS
|
5.7198
0.73261
1.6 0.0000
|
|
|
|
66
SC/M
TLMTP
SL/C
CDL
15
15
15
15
3.2460
45.946
3.9700
4.3780
0.91605
0.36882E-01
9.9011
0.53369
0.96585E-010.32558E-01
0.91476
0.94763E-01
1.1
1.2
0.8
2.2
0.0000
0.0000
0.0000
0.0000
Th nghim: nh hng ca t hp cht kch thch sinh trng kinetin v IBA

TLMS FILE D12
1/ 1/14 0:27
------------------------------------------------------------------ :PAGE
VARIATE V003 TLMS
LN
SOURCE OF VARIATION
DF
LN
SOURCE OF VARIATION
DF
LN
SOURCE OF VARIATION
DF
LN
SOURCE OF VARIATION
DF
LN
SOURCE OF VARIATION
DF
LN
SOURCE OF VARIATION
DF
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
4 1219.81
304.952
977.85 0.000 2
* RESIDUAL
10 3.11859
.311859
----------------------------------------------------------------------------* TOTAL (CORRECTED)
14 1222.93
87.3519
-----------------------------------------------------------------------------BALANCED
ANOVA FOR VARIATE
TLMTC FILE D12
1/ 1/14 0:27
------------------------------------------------------------------ :PAGE
2
VARIATE V004 TLMTC
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
4 543.227
135.807
136.55 0.000 2
* RESIDUAL
10 9.94588
.994588
----------------------------------------------------------------------------* TOTAL (CORRECTED)
14 553.173
39.5123
SC/M FILE D12
1/ 1/14 0:27
------------------------------------------------------------------ :PAGE
3
VARIATE V005 SC/M
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
4 12.4376
3.10941
442.93 0.000 2
* RESIDUAL
10 .702010E-01 .702010E-02
----------------------------------------------------------------------------* TOTAL (CORRECTED)
14 12.5078
.893417
TLMTPP FILE D12
1/ 1/14 0:27
------------------------------------------------------------------ :PAGE
4
VARIATE V006 TLMTPP
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
4 1407.89
351.974
****** 0.000 2
* RESIDUAL
10 2.09627
.209627
----------------------------------------------------------------------------* TOTAL (CORRECTED)
14 1409.99
100.714
SL/C FILE D12
1/ 1/14 0:27
------------------------------------------------------------------ :PAGE
5
VARIATE V007 SL/C
SUMS OF
MEAN
F RATIO PROB ER
SQUARES
SQUARES
LN
=============================================================================
1 CT
4 .923040
.230760
41.06 0.000 2
* RESIDUAL
10 .562001E-01 .562001E-02
----------------------------------------------------------------------------* TOTAL (CORRECTED)
14 .979240
.699457E-01
CDL FILE D12
1/ 1/14 0:27
------------------------------------------------------------------ :PAGE
6
VARIATE V008 CDL
SUMS OF
MEAN
F RATIO PROB
ER
67
SQUARES
SQUARES
LN
=============================================================================
1 CT
4 4.66344
1.16586
271.13 0.000 2
* RESIDUAL
10 .430002E-01 .430002E-02
----------------------------------------------------------------------------* TOTAL (CORRECTED)
14 4.70644
.336174
1/ 1/14 0:27
------------------------------------------------------------------ :PAGE
7
MEANS FOR EFFECT CT
------------------------------------------------------------------------------CT
1
2
3
4
5
SE(N=
5%LSD
NOS
3
3
3
3
3
3)
10DF
CT
1
2
3
4
5
TLMTC
34.0700
42.2200
52.5900
39.7367
43.2533
0.575786
1.81432
NOS
3
3
3
3
3
SL/C
3.45000
4.01000
4.19000
3.95000
3.99000
SC/M
1.43000
3.77000
3.82000
3.61000
3.58000
TLMTPP
29.2600
47.4100
59.2600
45.1900
49.2600
0.483739E-01 0.264340
0.152428
0.832944
CDL
3.60000
3.78000
4.98000
4.67000
4.76000
SE(N=
3)
0.432821E-01 0.378595E-01
5%LSD 10DF
0.136383
0.119297
1/ 1/14 0:27
------------------------------------------------------------------ :PAGE

VARIATE
TLMTC
SC/M
TLMTPP
SL/C
CDL
GRAND MEAN
(N=
15)
NO.
OBS.
15 42.374
15 3.2420
15 46.076
15 3.9180
15 4.3580
STANDARD
-------------------- SD/MEAN |
BASED ON
BASED ON
%
|
TOTAL SS
RESID SS
|
6.2859
0.99729
2.4 0.0000
0.94521
0.83786E-01 2.6 0.0000
10.036
0.45785
1.0 0.0000
0.26447
0.74967E-01 1.9 0.0000
0.57981
0.65575E-01 1.5 0.0000
|
|
|
|
68

Nghiên cứu nhân nhanh invitro cây chuối tiêu hồng

Uploaded by

Document Information

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

Nghiên cứu nhân nhanh invitro cây chuối tiêu hồng

Uploaded by

Copyright:

Available Formats

B GIO DC V O TO

TRNG I HC NNG NGHIP H NI

NGHIN CU NHN NHANH INVITRO

NGHIN CU NHN NHANH INVITRO

: CNG NGH SINH HC

NGI HNG DN KHOA HC

Trng i hc Nng nghip H Ni Lun vn thc s khoa hc Nng nghip

Trng i hc Nng nghip H Ni Lun vn thc s khoa hc Nng nghip

CHNG 1 TNG QUAN TI LIU

VI NT V PHN LOI V C IM CA CHUI

c im thc vt hc ca chi chui Tiu.

c dim thc vt hc ca chui Tiu Hng.

NG DNG CA K THUT NUI CY M T BO

Cc hng nghin cu ng dng

u th ca nhn ging in vitro

Cc phng thc nhn ging in vitro

Quy trnh nhn ging in vitro

MT S NGHIN CU NHN GING CHUI BNG K

CHNG 2 VT LIU V PHNG PHP NGHIN CU

VT LIU, A IM V THI GIAN NGHIN CU

Trng i hc Nng nghip H Ni Lun vn thc s khoa hc Nng nghip

a im v thi gian nghin cu.

PHNG PHP NGHIN CU

Phng php b tr th nghim

Phng php pha mi trng v nui cy

Phng php b tr th nghim

Phng php thu thp s liu v x l s liu

Phng php tnh ton kt qu

CHNG 3 KT QU V THO LUN

So snh nh hng ca mi trng nui cy MS, B5 v

nh hng ca cht kch thch sinh trng IAA n s pht sinh

nh hng ca cht kch thch sinh trng -NAA n s pht

nh hng ca cc cht kch thch sinh trng thuc nhm auxin

nh hng ca kinetin ln s pht sinh chi v to cm chi

nh hng ca BAP ti s pht sinh chi v to cm chi chui Tiu

nh hng ca cc cht kch thch sinh trng thuc nhm

nh hng ca cht kch thch sinh trng IBA n s hnh

Trng i hc Nng nghip H Ni Lun vn thc s khoa hc Nng nghip

nh hng ca t hp cc cht kch thch sinh trng thuc

nh hng ca t hp cht kch thch sinh trng kinetin v IAA

nh hng ca t hp cht kch thch sinh trng BAP v IBA

nh hng ca t hp cht kch thch sinh trng BAP v IAA

nh hng ca t hp cht kch thch sinh trng kinetin v IBA

nh hng ca gi th n s sinh trng ca chui Tiu Hng.

KT LUN V KIN NGH

TI LIU THAM KHO

Trng i hc Nng nghip H Ni Lun vn thc s khoa hc Nng nghip

Axit indol axetic

Axit indol butyric

Murashige and Skoog

Vacin and Went

Trng i hc Nng nghip H Ni Lun vn thc s khoa hc Nng nghip

So snh nh hng ca mi trng MS, B5 v Phytamax ti s

nh hng ca BAP ti s pht sinh chi v to cm chi chui

nh hng ca kinetin ti s pht sinh chi v to cm chi chui

nh hng ca -NAA ti s pht sinh r chui Tiu Hng

nh hng ca IAA ti s pht sinh r chui Tiu Hng

nh hng ca IBA ti s pht sinh r chui Tiu Hng.

nh hng ca t hp cht kch thch sinh trng BAP v IAA

nh hng ca t hp cht kch thch sinh trng kinetin v IAA

nh hng ca t hp cht kch thch sinh trng BAP v IBA

nh hng ca t hp cht kch thch sinh trng kinetin v IBA

Trng i hc Nng nghip H Ni Lun vn thc s khoa hc Nng nghip

So snh nh hng ca mi trng MS, Phytamax v B5 ti s