Professional Documents
Culture Documents
( Reaffirmed 1998 )
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Indian Standard
-METHOD OF LABORATORY TESTING OF
WOOD PRESERVATIVES AGAINST FUNGI
( First Revision 1
UDC
674-048.4 : 620.1
@ BIS 1993
BUREAU
MANAK
February 1993
OF
BHAVAN,
INDIAN
STANDARDS
9 BAHADUR
SHAH
NEW DELHI 110002
ZAFAR
MARG
Price Group 2
Timber Sectional
Committee,
CED 9
FOREWORD
This-Indian Standard ( First Revision ) was adopted by the Bureau of Indian Standards, after the
draft finalized by the Timber Sectional Committee had been approved by the Civil Engineering
Division Council.
Details of preservatives, methods of preservative treatment,
etc, have been covered in IS 401 :
1982 Code of practice for preservation
of timber ( third revision ). This standard covers the
method for the laboratory testing of wood preservatives against fungi.
This standard was first published in 1968. Based on the experience gained in the use of this
standard, this revision has been brought out. Both the test fungi for preparation of test culture
in case of non-conifers mentioned in the earlier version, namely, Polyporous
versicolor ( Linn. )
Fr and Polystictus sanguineus ( L. ) Mev. were white rot fungi. Now Gloeophyllum trabeum ( Pers.
ex. Fries ) Murr. has been added which is very aggressive brown-rot fungus against wood.
In reporting the result of a test or analysis made in accordance
with this standard, if the final
value, observed or calculated, is to be rounded off, it shall be in accordance with IS 2 : 1960
Rules for rounding off numerical values ( revised).
IS 4873 : 1993
Indian Standard
METHOD OF LABORATORY TESTING OF
WOOD PRESERVATIVES AGAINST FUNGI
( First Revision )
1 SCOPE
GC
( R ) = =g/cm3
be converted
( this may
where
G = the mass of the treating
SdUtiOn
absorbed by block ( W, - WI ) in g,
C = the
mass
present
and
in
in g of the preservative
100 g of the treating solution,
IS 4873 : 1993
The treated blocks shall then be conditioned
at_3OoC and 70 f 4 percent relative humidity
to a constant mass W,.
2.6 Preparation of Soil Culture Bottles
Sifted ( sieved ) air dried garden soil amounting to 125 g withpH between 5.0 and 7.0 shall
be filled ( compacted by tapping ) in ( 237 ml )
aluminium screwing capped bottles.
Distilled
water is added to this so as to obtain 130
percent of water holding capacity of soil in
test bottles. Two feeder blocks are then kept on
the surface of the soil, of the size mentioned
above.
Sterilize the prepared
bottles
with
caps loosened out 0.1 N/mm* for 60 minutes on
two consecutive days.
2.7 Preparation of Test Culture
After the sterilized culture bottles are thoroughly
cooled, cut the gungus inoculum ( see 2.7.1 )
approximately
10 mm square
from a not
more than 3-week old culture and place on the
edge of the feeder blocks.
Incubate the inoculated bottles with slightly loosened lids at
70 f 4 percent
relative
25C f 1C and
humidity
for approximately
3 weeks. The
bottles are then ready to receive the test blocks.
Mass loss,
W, = condition
test, and
W, = condition
test.
2.11 Threshold
b) Poria monticola
Murr.
particularly
tolerant to copper and zinc compounds.
of this fungus
of arsenic.
are
resistant
:o
mass of the
mass
of
block
the block
before
after
Retention
trabeum
= Jv,
--wp- w, x 100
3
where
b) Gloeophyllum
Murr.
percent
3 ALTERNATIVE METHOD
( KOLLE-FLASK ) METHOD
3.1 Preparation
AGAR-BLOCK
of Test Blocks
high
1s 4873 : 1993
3.2 Conditioning
3.6 Infection
of Test Blocks
The procedure
3.3 Treatment
Procedure
The procedure
and 2.4.
is the
same
as given
in 2.3
fungi
shall be the
same
as given
of Test Blocks
and Duration
The procedure
of Test
3.8 Calculation
of Weight Loss
The procedure
3.9 Threshold
The procedure
Retention
IStandardTheMarkuse of the
of Indian
Standards
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Standards
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a standard
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Users of Indian
Standards
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Comments
on this Indian Standard
may be sent to BIS giving the following
reference:
Dot : No
CED 9 ( 4874 )
Amendments
Amend
No.
Date
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