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TABLE OF CONTENTS
Overview.................................................................... 2
Getting started........................................................... 2
1. Unpacking a new electrode .............................................. 3
2. mounting of the electrode and reference ......................... 3
3. Connecting the redox electrode........................................ 3
4. Calibration........................................................................ 3
Measurements........................................................... 4
Overview
The Unisense REDOX
microelectrode is a miniaturized
platinum electrode designed for
research applications within
environmental sciences, and
related areas.
Getting started
The signal from the redox
electrode is read on a high
impedance milivolt meter like
the Unisense PHM210. Therefore
Unisense A/S
Denmark
www.unisense.com
WARNING
Do not let the
microelectrode
tip touch
anything during
handling it is
very fragile.
1. Unpacking a new
electrode
WARNING
Do not remove seal and
protective plastic tube before the
following steps (1-4 are
successfully completed
Replacement of electrodes
Unisense will replace electrodes
that have been damaged during
shipment provided that:
4. Calibration
Prepare quinhydrone redox
buffer solutions:
Mix 1 l of buffer solution (pH 4
or pH 7) with 10 g of
quinhydrone. Crystals of
quinhydrone should be present
indicating that the solution is
saturated. Quinhydrone is a
mixture of equal amounts (molar
basis) of hydrochinone and
quinone.
Unisense A/S
Denmark
www.unisense.com
Construction of
the electrode
with an internal
reference
involves
creation of a
liquid junction
approximately 3
cm from the
electrode tip.
During
measurements
this liquid
junction has to
be submersed in
the sample in
order to
complete the
measurement
circuit
micromanipulator MM-33
(MM33-2 or MMS) The Unisense
REDOX microelectrode tip is
surprisingly flexible and may
bend slightly to find its way
around physical obstacles. But
coarse lateral movements of the
electrode when its tip is in
contact with a solid substrate
may easily cause the tip to
break.
Measurements
Mounting of the electrode
During measurements the redox
electrode should be properly
mounted in a stable set-up. We
recommend the Unisense lab
stand LS18 and the Unisense
Unisense A/S
Denmark
www.unisense.com
pH 4
buffer
pH7
buffer
IMPORTANT
As the Redox
microelectrode
is sensitive to
temperature, it
is necessary to
perform
calibration at
the same temperature as your
measurements
20
470
295
25
462
285
30
454
275
Cleaning the
electrode
The electrode can by cleaned by
immersing it in nitric acid (1+1)
Keep the electrode in the nitric
acid solution for about 30
minutes and allow it to cool and
rinse with water afterwards.
Advanced redox
electrodes
Unisense can construct redox
electrodes for customer
requested applications at
additional costs. The most
frequently construction options
are described at our web page
under redox electrode
specifications
Electrical noise
As well as the physical size, the
electrical current generated by
the high-impedance
microelectrode is very small.
Although the Unisense REDOX
microelectrodes are very
resistant to electrical noise from
the environment, electrical fields
may interfere with the electrode
signal. Minimize this by
switching off unnecessary
electrical/mechanical equipment
and by not touching electrode or
wires during operation.
http://www.unisense.com/support/rd_spec.h
tml
On suspicion of electrode
damage, repeat calibration and
consult Trouble-shooting.
References
WARNING
Always
introduce and
retract the
redox
microelectrode
axially using a
micromanipulator and a stable
stand when
measuring in
solid or
semisolid
substrate like
sediment,
tissue, biofilms,
microbial mats
etc.
Trouble shooting
Problem:
Possible cause:
Solution:
Problem:
Possible cause:
Solution:
Problem:
Possible cause:
Solution:
Storage and
maintenance
Temperature
Keep good track of the
temperature, as the electrode
signal is very dependent on
temperature.
Unisense A/S
Denmark
www.unisense.com
If you encounter
other problems
and need
scientific/technic
al assistance,
please contact
sales@unisense.com
for on-line
support (we will
answer you
within one
workday)