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ABSTRACT
Most poisoning cases are associated with excessive exposure to toxic chemicals such as household
materials. A bioassay using Artemia salina (brine shrimp) was adapted to measure the toxicity of Lysol.
Appropriate amount of Lysol (0.001%, 0.01%, 0.1% to give concentration of 10 g/ml, 100 g/ml, 1000
g/ml respectively) were transferred into vials containing brine shrimp medium. The procedure determines
the LC50 values in g/ml of active compounds of Lysol in the said medium. Activities of active compounds
are manifested as toxic to brine shrimps. The lethal concentration of Lysol resulting 50% mortality of the
brine shrimp LC50 was determined from 6 and 24 hours. The vials were then inspected after. The number
of surviving larvae were counted. The percentage of mortality was calculated at each concentration then
concentration mortality data were analyzed statistically. The concentration at which 50% of the population
died after 6 hours of exposure to Lysol was 100g/mL. Further, after 24 hours of exposure to Lysol, all
died. Since there is good correlation between LD50 using brine shrimp bioassay and LD50 reported in mice,
it can be said that the lethal concentration of Lysol in rats when taken orally after a six-hour exposure is
approximately 100g/mL.
BACKGROUND INFORMATION
Poisoning is a significant global public
health problem. According to WHO data, updated
2016, in 2012 an estimated 193,460 people died
worldwide from unintentional poisoning. Of
these deaths, 84% occurred in low- and middleincome countries like the Philippines. In the same
year, unintentional poisoning caused the loss of
over 10.7 million years of healthy life (disability
adjusted life years, DALYs). It said that most of
the poisonings are associated strongly with
excessive exposure to, and inappropriate use of,
toxic chemicals such as the household material,
Lysol. In a report by CBC Marketplace,
Physical characteristics
Sam
ple
Lysol
REFERENCES
Vial 1
(10g/mL)
6hrs
12.5%
24hrs
100%
Mortality (%)
Vial 2
(100g/mL)
6hrs
50%
24hrs
100%
Vial 3
(1,000g/mL)
6hrs
100%
24hrs
100%
CONCLUSION
Since there is a good correlation between
LD50 using brine shrimp bioassay and LD50
reported in mice (Logarto et al, 2001), it can be
said that the lethal concentration of Lysol in rats
when taken orally after a six-hour exposure is
approximately 100g/mL. However, the results
collected may not be applicable to humans.