University of Santo Tomas

Pharmaceutical Botany Laboratory

1A PH
Tolentino, Umali, Zacarias, Zamora, Zaraspe

TRANSPIRATION
I. Introduction
V. Results
Transpiration is the process by which water is
lost from a plant primarily in the form of water
vapor. It occurs through the stomatal pores of the
leaves and insignificantly through the surface of
leaves through the cuticle and lenticels. It uses
about 90% of the water that enters the plant and
the other 10% is used in photosynthesis and cell
growth. Transpiration serves three essential roles
it moves water and nutrients up from the root
throughout the plant, gives the plant an
evaporative cooling effect, and secures turgidity.
II. Objectives
To explain how water cycles through
plants in the transpiration process
To describe the relationship between
environmental factors and the rate of
transpiration in plants
III. Materials
Woody dicot twig
(Tabernaemontana pandacaqui)
Bell jar
Rubber tubing
Stool with a small
hole in the center
Iron stand
Iron clamp
Burette
IV. Procedures

After 1 day
13 mL of water was absorbed by the plant
There were water droplets around the bell jar
Explanations:
In this experiment, moisture or water droplets
can be found around the bell jar after an overnight
exposure of the plants. These droplets are formed
through the condensation of water vapor that was
released by the stoma of the plants through the
process of transpiration. In order to replace the
loss of water in the leaves, the tip of the plant that
is connected to the rubber tubing absorbs water
from the burette and transports it to the leaves.
This is the reason why the water level on the
burette decreased overnight and the plant did not
wither even though it is tightly enclosed inside the
glass bell jar. If water loss is not replaced, the
absorption of water by the roots will not keep up
with the rate of transpiration. Thus, the plant will
wilt.
VI. Conclusion
Water is absorbed by the plant and through
capillary action, it is transported to all parts of the
plants. However, the plant for photosynthesis and
other chemical reactions uses not all water.
Therefore, excess water is transpired and loss

through the process of transpiration. Transpiration

brings about coolness and turgidity to plants.
Environmental factors also affect the rate of
transpiration in plants. Exposure to light, higher
temperature, lower relative humidity and the
presence of wind would faster the transpiration
rate.
VII. References
[1] Vasquez, R., Laurente, O., Cobar, M. Laboratory
Manual for Pharmaceutical Botany. Espaa, Manila:
University of Santo Tomas.
[2] Mauseth, J. (2013). Fifth Edition Botany: An
Introduction to Plant Biology, Philippine Edition. Quezon
City, Philippines: Mind Mover Publishing House, Inc.
[3] Sterling T.M. (2005). Transpiration - Overview and
Objectives. Plant & Soil Sciences eLibrary. Retrieved from:
http://passel.unl.edu/ pages/informationmodule.php?
idinformation
module=1092853841