TRNG I HC Y DC THNH PH H CH MINH

KHOA DC
B MN BO CH

CNG THC TP BO CH 1
PHN 1

DUNG DCH THUC

V HA TAN CHIT XUT
(IU CH SIRO TR HO)

t Thc Tp: 2
Nhm Thc Tp: 1
Ngy Thc Tp: Sng Th 2 v Sng Th 4
Bn Thc Tp: 4 - Tiu nhm: 8
Lp: VB2-2014
NH: 2015-2016

Thng 06/2016

Cng thc 1 n v v 4 n v sn phm siro tr ho

STT
1
2
3
4
5
6
7
8
9

Thnh phn
Dung dch Bromoform dc dng
Cn Aconite
Eucalyptol
Siro hng chanh
Nc bc h
Acid citric
Natri benzoate
Nc tinh khit
Siro v qut (v)

1 n v sn phm
0,6 g
0,6 g
0,01 g
12 g
5 ml
0,1 g
0,1 g
2 ml
100 g

4 n v sn phm
2,4 g
2,4 g
0,04 g
48 g
20 ml
0,4 g
0,4 g
8 ml
400 g

BC 1: IU CH NGUYN LIU
1. Cn Aconit
1.1. Cng thc v cch iu ch [2]
Cng thc cn aconit vi lng dc liu l 40 g
u, bt mn va
40 g
Cn 90o
va
320 g
Cch iu ch: Chit bng phng php ngm kit
Lm m 40 g bt u trong 20 ml cn 90 o trong becher 250 ml, bao kn bng nilon.
yn khong 2 gi
Giai on ngm lnh: cho bt u lm m vo bnh ngm kit lt bng v giy
lc y. Cho cn 90o vo t t (bng ng ong) n khi ngp mt dc liu khong 1,5
cm. Ngm 24 gi.
Rt dch chit vi tc 1 ml/pht (khong 1git/ 3giy). Thm cn 90 o trong khi rt
dch chit sao cho cn lun ngp mt dc liu. Tng lng cn 90 o thm vo rt dch
chit l 250 ml ( lng dch chit cn rt).
Thm dung mi v tip tc rt dch chit cho n khi thu c 250 ml th khng thm
dung mi na.
Rt ht dch v p b.
Trn dch chit vi dch p, nu cn b sung thm cn 90o va 320 g.
nh lng alkaloid trong dch chit.
Lc sau 3 ngy yn trong mt.
1.2. Cc c im ca dc liu [2], [4]
u l r c ca cy u (Aconitum fortune
Ranunculaceae)
R c hnh c u hay hnh con quay. Mt ngoi mu
nu hay nu en, c nhiu np nhn dc v vt tch
ca r con ct ra. Cng chc, rn v dai, kh b,
vt ct mu nu xm nht. V nht sau hi cht v
hi t li.
u chnh l c ci khng qua ch bin, rt c
thng ch dng ngoi
1

Ph t l c con c qua ch bin, t c hn dng ng ung

u l mt dc liu c, khng tan trong nc, tan trong cloroform hay benzen, cn,
ete.
Aconitine
Thnh phn ha hc chnh ca u l alkaloid aconine, aconitine.
i vi dc liu c th dung mi chit xut thng l ethanol 70 %, nhng ring u
ta dng ethanol 90 % v hot cht trong u l aconitin d b thy phn lm gim c
tnh ca u xung 1000 ln, nn gi hot tnh ca u phi chit bng cn 90o.
Aconitin d b thy phn thnh acid acetic v benzoylaconitin, lm gim tc dng dc l
ca cn u.
1.3. S xc nh nhanh gii hn alcaloid trong cn aconit [4]
Theo phng php Debreuill
Dch chit cn u

Mu A 20 g

Mu B 20 g

C cch thy

C cch thy
Cn B

Cn A
1 ml HCl 10%
20 ml nc ct
4,5 ml TT Mayer 10%
Lc
Dch lc A

1 ml HCl 10%
20 ml nc ct
5,5 ml TT Mayer 10%
Lc
Dch lc B

1 ml TT Mayer 10%

1 ml TT Mayer 10%
Khng ta

Ta

Kt qu: 1 ml thuc th Meyer 1/10 tng ng 0,0021 g aconitin.

Nu mu A c ta, mu B khng ta: t, lng alkaloid ton phn tnh theo aconitin nm
trong khong gii hn cho php (0,045 0,055 %).
Nu mu A v mu B u c ta: Khng t, chng t hm lng alkaloid qu m c, phi
pha long.
Nu mu A v mu B u khng c ta: Khng t, chng t hm lng alkaloid qu thp,
cn phi chit thm l khc m c hn pha trn 2 mu nhm t c hm lng
alkaloid mong mun.
2

1.4. Cc tiu chun kim nghim ca cn Aconit [3]

Cm quan: L cht lng mu vng nu nht, v ng v gy cm gic kin cn trn u li.
T trng 250C: 0,825 0,855.
c tnh: c ca 1 ml cn thuc phi tng ng vi c ca 0,15 mg aconitin chun
hoc LD50 cho mi kg th trng chut phi xp x 2,4 ml (gii hn trn v di 1,6 v 3,2 ml).
nh lng
Cn chnh xc 100 g ch phm, un cch thy v sy cho bay hi cn. Ha tan cn
bng cch nghin nh vi 25 ml dung dch acid sulfuric 1% v lm tip 3 ln, mi ln vi dung
dch acid sulfuric 0,1N. Lc cc dch acid qua bng vo mt bnh gn. Kim ha bng dung
dch ammoniac (TT) v lc nhiu ln, mi ln vi mt lng nh chloroform ti khi chit ht
alkaloid. Ra mi dch chloroform vi cng mt lng 20 ml nc. Tp trung cc dch
chloroform un cch thy nh cho bay hi chloroform, thm 5 ml eter (TT) vo cn ri lm bay
hi. Gi cn thu c 60 oC trong vng 1 gi. Ha tan cn vi 20 ml dung dch acid sulfuric
0,02N. nh lng acid tha bng dung dch natri hydroxid 0,02N, ch th l methyl .
Gi n l s ml dung dch natri hydroxid 0,02N dng.
1 ml dung dch acid sulfuric 0,02N tng ng vi 0,01292 g alkaloid, tnh theo
aconitin, (C34H47O11N).
Hm lng phn trm alkaloid ca ch phm em th tnh theo cng thc.

(20 n). 0,01292

Cn u phi cha 0,045 0,055 phn trm alkaloid ton phn, tnh theo aconitin,
(C34H47O11N).
1.5. Uc lng lng cn 90o cn s dng chit xut:
Theo thc t lng cn thuc cn thu c l khong 320 g. V vy lng ethanol 90 o
cn dng phi t nht l 500 600 ml (tr hao lng dung mi lc lm m v dung mi b gi li
trong dc liu).
2. Siro hng chanh [2]
Siro hng chanh c iu ch t nguyn liu: l hng chanh ti, ng, nc ct.
Theo l thuyt iu ch 4 n v thnh phm th cn siro hng chanh l 48 g, thc t nhm s
pha 60 g siro hng chanh do tr lng hao ht trn dng c.
Dch chit hng chanh:
- c 60 g siro cn lng dch chit l 21,5 ml (hoc 21,5 g).
60 . 1 00
m =
= 21,429 g
280
- Bit 0,8 ml dch chit tng ng vi 1 g dc liu (t trng dch chit xem nh bng 1).
21,5 . 1
m =
= 26,875 g
0,8
Vy iu ch 21,5 g dch chit ta cn lng l hng chanh l 27 g l.
Cng thc iu ch 21,5 ml dch chit hng chanh l
L hng chanh ti
27 g
Nc ct
30 ml
Siro hng chanh:
- Ha tan ng vo dch chit thu c siro hng chanh
60 . 180
m=
= 38,571 g
280
3

 Vy cn thm 38,5 g ng vo dch chit hng chanh.

Cng thc iu ch 60 g siro hng chanh l
Siro n pha ch ngui [2]
ng
Nc
Siro thu c

180 g
100 g
280 g

Siro hng chanh (60 g)

ng
Dch chit hng chanh
Siro thu c

38,5 g
21,5 g
60 g

3. Nc bc h
Cng thc v cch iu ch 50 ml (hoc 50 g) nc thm bc h t nguyn liu tinh du bc h
theo phng php.
3.1. Dng cht trung gian ha tan
iu ch nc thm bc h dng bt Talc lm cht trung gian ha tan [2]
Cng thc gc
Tinh du bc h
Bt Talc
Nc ct v

Cng thc thc t

:1g
: 10 g
: 1000 g

Tinh du bc h
Bt Talc
Nc ct v

: 0.05 g
: 0,5 g
: 50 g

iu ch: Cn 0,05 g tinh du bc h nghin vi bt Talc, sau thm nc ct va 50 g,

khuy k. yn 24 gi, thnh thong khuy, sau lc qua giy lc thm t.
Dng cht din hot Tween lm cht trung gian ha tan [2]
Cng thc gc
Tinh du bc h
Tween 20
Ethanol 90%
Nc ct

Cng thc thc t

:2g
: 20 g
: 200 g
: 778 g

Tinh du bc h
Tween 20
Ethanol 90%
Nc ct

: 0,1 g
:1g
: 10 g
: 38,9 g

iu ch: Cn 0,1 g tinh du bc h v 1 g Tween 20 cho vo erlen c nt mi, y np v lc

u. Cn 10 g ethanol 90% vo erlen trn lc u, thm nc ct va 50 g lc u tip. Lc
qua giy lc thm nc. ng chai, dn nhn, bo qun.
Dng ethanol 90 % lm cht trung gian ha tan [2]
Giai on 1: Ha tan trong ethanol
Tinh du bc h
: 0,1 g
Ethanol 90 % v
: 10 g

Giai on 2: Ha tan trong nc

Dung dch giai on 1
:1,5 g
Nc ct v
: 50 g

3.2. Phng php ghi trong Aromatic water, sch Remington [7]
Cng thc gc
Cng thc thc t
Tinh du bc h
Ethanol 90 %
Bt Talc
Nc ct v

: 20 ml
: 600 ml
: 50 g
: 1000 ml

Tinh du bc h
Ethanol 90 %
Bt Talc
Nc ct v

: 1 ml
: 30 ml
: 2,5 g
: 50 ml

iu ch: ong 30 ml ethanol 90 % cho vo erlen c nt mi, thm 1 ml tinh du bc h vo

erlen trn y np lc u. Thm t t nc ct va 50 ml, lc mnh. Trn 2,5 g bt Talc vo
hn hp trn, lc u sau yn vi gi. Lc qua giy lc thm nc.
4. Siro v qut
Siro v qut c iu ch bng cch trn 1 phn dch chit m c v qut vi 9 phn siro n
[2]. Cng thc iu ch 400 g siro v qut.
Dch chit m c v qut
40 g
Siro n
360 g
V trong dch chit m c v qut c 1 phn siro n nn trong qu trnh iu ch cn tnh
thm lng siro ny.
Lng siro iu ch = Siro n (360 g) + Lng thm vo dch chit m c
4.1. Dch chit m c v qut
Cng thc iu ch 40 g dch chit m c v qut [2]
Cng thc gc
Cng thc iu ch
V qut ct nh
30 g
V qut ct nh
12 g
Ethanol 80%
30ml
Ethanol 80%
12 ml
Ethanol 90%
30 ml
Ethanol 90%
12 ml
Nc
300 ml
Nc
120 ml
Siro n
va 100 g
Siro n
va 40 g
S iu ch 40 g dch chit m c v qut [2]
12 g v qut tm 12 ml ethanol 80%, 12 gi
80 ml nc 80oC, 12 gi, gn lc

Dch chit 1

Dc liu chit ln 1

Ct cn thm

8 ml cn thm

40 ml nc 80oC
6 gi, lc

Dch chit ct cn

Dch chit 2

Hn hp DC
C cch thy
12 ml dch chit m c
+ 12 ml cn 90%, lnh 12h, gn lc
Dch chit m c loi tp cht (quy th tch v khi lng)
+ Siro n v (ghi li lng siro thm vo)

Dch chit m c v qut (40 g)

Tnh cht ca ch phm: [2]

- Dch chit m c l dng bo ch trung gian c pha ch sn v bo qun trong phng
pha ch.
- Khi cn pha siro thuc th trn 1 th tch dch chit m c vi 9 th tch siro n.
- u im ca dch chit m c l cho php iu ch ch phm t dc liu nhanh chng
m khng cn bo qun dc liu phng pha ch.
- Dch chit m c c tiu chun ha v mt ha hc, vt l nn cc thnh phm thu
c t dch chit m c s ng nht v mt cht lng, t ph thuc vo dc liu.
4.2. Siro n
Bn cng thc tng ng vi lng ng 320 g
Tn CT
Cng thc ngh
Siro pha Cng thc gc
ngui
ng saccarose 180 g
[1],[3]
Nc ct
100 g
Cch tnh ton
180 g ng c 280 g siro
320 g ng 498 g siro
180 g ng tan /100 g nc
320 g ng 178 g nc
ng
320 g
Nc
178 g
Siro thu c 498 g
Siro pha Cng thc gc
nng
ng saccarose 165 g
[1],[3]
Nc ct
100 g
Cch tnh ton
100 g siro c 64 g ng
500 g siro 320 g ng
165 g ng tan /100 g nc
320 g ng 194 g nc
ng
320 g
Nc
194 g
Siro thu c 500 g
Siro
Cng thc gc
theo
ng
850 g
USP
Nc v
1000 ml
[8]
Cch tnh ton
1000 ml c 850 g ng
377 ml 320 g ng
ng
320 g
Nc ct v 377 ml
Siro thu c 377 ml
(~500 g)

T trng

1,32
(20oC)

1, 26
(105oC)

Cch pha
Ha tan 320 g ng vo becher cha 178 g
nc, khuy cho tan hon ton, c th gia
nhit (<60 C) gia tng tc ha tan. Lc
trong qua ti vi. iu chnh (nu cn) c
t trng quy nh.

un 194 g nc n khong 70-80C , cho

320 g ng vo va un va khuy cho
ng tan hon ton, tip tc un n 105C.
Lc nng qua ti vi. iu chnh (nu cn)
c t trng quy nh.

1,32
(20oC)

1,30

Siro n c iu ch t nc si tt hn,
khng cn gia nhit.
Cho 320 g ng vo bnh ngm kit thch
hp c lt sn lp bng c thm nc
phn nn ct ca bnh. Cho t t 200 ml nc
si ln lp ng, iu chnh dng chy n
khi n nh. C th ngc phn siro thu
c vo bnh v lm nh trn n khi ng
tan hon ton. Sau ra v trng bng vi
nc va 377 ml.

Siro
Cng thc gc
theo BP ng
667 g
[9]
Nc v
1000 g
Cch tnh ton
1000 g siro n c 667 g ng
479 g siro
320 g ng
ng
320 g
Nc ct v 479 g
Siro thu c 479 g

un nng 320 g ng v lng nc ct

thch hp cho n khi tan hon ton, thm
nc ct un si va 479 g.
1,315
1,333

5. Ethanol [2]
Pha 500 ml ethanol 90% t ethanol nguyn liu
Cch pha v tnh ton gm 4 bc
Bc 1: Xc nh nng cn nguyn liu
Dng cn k xc nh cn 15 oC s bit c cn tht. Nu o cn nhit
khc th l cn biu kin. i t cn biu kin sang cn tht th phi tra bng
Gay-Lussac (nh km):
- Nu cn biu kin ln hn 56%: p dng bng Gay-Lussac
- Nu cn biu kin trong khong 25 56%: p dng cng thc sau tnh cn tht.

X=C+0,4(15-t)
Trong :

X: l cn tht
C: cn biu kin
t: nhit ca cn ti thi im o
Bc 2: Tnh ton lng cn nguyn liu
Cn nguyn liu c nng ln hn 90%: pha long cn cao vi nc to cn
90%
p dng cng thc: C1.V1=C2.V2
Trong :
C1,V1 ln lt l nng v th tch cn cao
C2,V2 ln lt l nng v th tch cn mun pha
Theo yu cu th C2 = 90%; V2= 500ml
Vy th tch cn cao cn ly l: (90x500)/C1 (ml)
Bc 3: Pha cn theo s liu tnh
Bc 4: Kim tra li nng cn mi pha (bng cn k v tra bng Gay-Lussac).
Nu sai bit qu gii hn cho php th phi chnh li.

BC 2: IU CH SIRO HO
1. Cng thc hon chnh 4 n v siro tr ho
STT
1
2
3
4
5
6
7
8
9

Thnh phn
Dung dch Bromoform dc dng
Cn Aconite
Dung dch Eucalyptol 10% (kl/kl)
Siro hng chanh
Nc bc h
Acid citric
Natri benzoate
Nc tinh khit
Siro v qut (v)

1 n v sn phm
0,6 g
0,6 g
0,1 g
12 g
5 ml
0,1 g
0,1 g
2 ml
100 g

4 n v sn phm
2,4 g
2,4 g
0,4 g
48 g
20 ml
0,4 g
0,4 g
8 ml
400 g

2. c im ca cng thc
-

Ch phm siro thuc dng lng, c cu trc dung dch.

Gm c cc cht to ngt n: siro hng chanh, siro v qut hm lng ng
saccarose cao th cht c snh, v ngt.
Cha cc dc cht dng iu tr ho: u, hng chanh, v qut [1]

3. Tnh cht, vai tr ca cc cht trong cng thc

ST
T

Tn cht

Tnh cht

Vai tr

Dung dch
Bromoform
dc dng [6]

Dung dch trong, khng mu, c L hot cht, c tc dng chng co

mi v v ngt - t li (v tht ng h hp, lm du v lm
bromoform). D tan trong nc, t gim cn ho.
trng 20oC 1.

Cn Aconit [3]

Cht lng, mu vng nu nht, v

ng. Nu thm cng mt khi
lng nc th dung dch tr nn
c. T trng 25oC: 0,825 0,855.

Eucalyptol [5]

Cht lng, trong, khng mu, mi L hot cht, cht bo qun, to mi

c trng, khng tan trong nc, cho siro; c tnh st trng ng h
ha tan c vi ethanol 96% v hp, cha ho.
dichloromethan, t trng 20oC:
0,920 0,926.

Siro hng chanh Cht lng snh, c mi thm, v cay. L hot cht, cng dng cha cm
[11]
cm, ho st do phong hn, ho g, khan
ting.

Nc bc h
[12]

L hot cht, c tc dng gim au,

gim vim trong vim ph qun, kch
thch h hp, cha ho, chng tit m
hi.

Cht lng trong hoc hi c, mi Cht to mi thm, c cng dng st

c bit ca tinh du bc h.
khun ng h hp, gim ho, gim
8

ri lon h hp, lm thng mi hng,

ph qun
6

Acid citric [14]

Bt kt tinh trng, d tan trong nc Cht chng oxy ha, chnh pH, to v
v ethanol, hi tan trong ether, t chua, cht to phc bt hot cc ion
trng: 1,665 g/cm3
kim loi.

Natri benzoate Bt kt tinh trng, d tan trong Cht trung gian thn nc ha tan,
[15]
nc, hi tan trong ethanol 90%, tc dng kim khun, cht bo qun
nng s dng khng qu 0,3 % . chng s pht trin ca vi khun v
nm mc

Nc ct [16]

Cht lng trong sut, khng mu,

khng mi, khng v, l dung mi
phn cc mnh, khng ha tan c
cht bo, nha, alcaloid base.

Siro v qut
[13]

V ngt, cht lng snh, hi c, Lm nguyn liu pha siro, cht lm

mu vng nht, mi thm ca v thm, c cng dng tr ho.
qut. T trng 25oC: 1.26 - 1.32.

Dung mi iu ch siro hng

chanh, nc bc h, siro n, siro v
qut, ha tan acid citric v natri
benzoat.

4. Cch iu ch
4.1. Chun b dng c v nguyn liu:
- Becher, erlen, ng ong, cn in t, a khuy...
- Cc nguyn liu: cn Aconit, siro hng chanh, nc bc h, siro v qut
- iu ch dung dch Eucalyptol 10% (kl/kl)
V khng th cn c chnh xc 0,04g Eucalyptol, nn nhm ngh pha dung dch Eucalyptol
10% (kl/kl).
Eucalyptol
Dung dch Eucalyptol 10% (kl/kl)

1 n v sn phm
0,01 g
0,1 g

4 n v sn phm
0,04 g
0,4 g

Cng thc pha ch nh sau: cn chnh xc 0,5 g Eucalyptol v 4,5 g ethanol 90%, cho vo erlen
kh sch, lc u. Sau , ng trong l nt kn, dn nhn, bo qun ni kh ro, thong mt.
Eucalyptol
Ethanol 90%

0,5 g
4,5 g

4.2. iu ch (cho 4 VSP)

Becher 1

Becher 2
Becher 3

Pha nguyn liu tan trong cn.

Dung dch Eucalyptol 10% (kl/kl):
Dung dch Bromoform dc dng:
Cn Aconit:
Siro hng chanh:
48 g
Pha acid citric
9

0,4 g
2,4 g
2,4 g

Becher 4
Becher 5

Acid citric:
Nc ct:

0.4g
4ml

Pha natri benzoat

Natri benzoate:
Nc ct:
Nc bc h:

0.4g
4ml
20ml

Tin hnh iu ch:

- Cho t t hn hp becher 1 vo erlen 1000 ml sch v kh cha sn 200g siro v
qut, khuy u.
- Tip tc cho t t 48g siro hng chanh becher 2 vo erlen, khuy u.
- Sau , cho dung dch acid citric becher 3 v dung dch natri benzoat becher 4 vo
erlen, khuy u v k.
- Cui cng cho 20ml nc bc h vo, dng siro v qut trng tt c cc becher, lng
siro cho vo va 400 g, khuy u n khi hn hp ng nht.
- o t trng thnh phm bng t trng k, nu kt qu nm trong khong 1,26 1,32 l
t.
4.3. ng chai v bo qun
ng chai:
- Chun b: 4 chai thy tinh loi 100ml, ng ong.
- T hn hp siro 400g, chia u vo 4 chai thy tinh, mi chai 100g.
- y kn nt, dn nhn.
Bo qun: ni thong mt, trnh nh sng trc tip.
5. V nhn
Nhn ca 1 n v thnh phm
TRNG I HC Y DC TP.HCM
KHOA DC - B MN BO CH
41- 43 inh Tin Hong, P. Bn Ngh, Q.1, TP.HCM

SIRO TR HO
Chai 100 g
Cn Aconit..................................................................0,06 g
Eucalyptol...................................................................0,01 g
Siro hng chanh.............................................................12 g
Nc bc h...................................................................5 ml
Siro v qut v t dc (v).........................................100g
S l SX: 0716
SK: VD072016
Ngy pha ch: 19/07/2016
Ch nh: Tr ho
HSD: 19/07/2017
Bo qun: xem t HDSD
xa tm tay tr em, c k hng dn trc khi s dng
10

11

TI LIU THAM KHO

[1]. L Quan Nghim, Dung dch thuc, Bo ch v sinh dc hc, NXB Y Hc, 2014, tr 44106.
[2]. L Th Thu Vn, Cc dng thuc bo ch bng phng php ha tan chit xut, Bo ch
v sinh dc hc, Tp 1, NXB Y Hc, 2014, trang 256 - 262, 280, 295 - 297, 302 - 309.
[3]. B Y t, Chuyn lun Cn u, Dc in Vit Nam I, NXB Y Hc, 1971, tr. 176-177.
[4]. B Y t, Chuyn lun dc liu u, Dc in Vit Nam IV, NXB Y Hc, 2009.
[5]. Eucalyptol (https://en.wikipedia.org/wiki/Eucalyptol).
[6]. B Y t, Chuyn lun Dung dch Bromoform dc dng, Dc in Vit Nam I, tr.210.
[7]. Phillip P. Gerbino, Aromatic water, Chapter 39, Remington - The Science and Practice of
Pharmacy 21st Edition, 2005, p.749.
[8]. Syrup, USP 30 NF 25, p. 1233.
[9]. Syrup, Formulated Preparations, British Pharmacopoeia 2013.
[10]. B Y t, Chuyn lun Siro n, Dc in Vit Nam IV, NXB Y Hc, 2009, tr. 257.
[11]. B Y t, Chuyn lun Siro hng chanh, Dc in Vit Nam III, NXB Y Hc, 1971,
tr.381.
[12]. B Y t, Chuyn lun Nc Bc h, Dc in Vit Nam I, NXB Y Hc, 1971, tr..
[13]. B Y t, Chuyn lun Siro v qut, Dc in Vit Nam I, NXB Y Hc, 1971, tr.649.
[14]. B Y t, Chuyn lun Acid citric, Dc in Vit Nam IV, NXB Y Hc, 2009.
[15]. B Y t, Chuyn lun Natri benzoat, Dc in Vit Nam IV, NXB Y Hc, 2009.
[16]. B Y t, Chuyn lun Nc ct, Dc in Vit Nam IV, NXB Y Hc, 2009.

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K HOCH THC HIN

Bui 1

Bui 2

Bui 3
Bui 4

- iu ch ethanol 90o.
- iu ch siro n.
- iu ch siro v qut: Tm cn 80% vo v quit, 12h
- iu ch cn aconit: Lm m bt u.
Cho bt u lm m vo bnh ngm, thm dung mi.
- iu ch siro v qut:
Ct cn thm, chit dch chit m c.
Hon chnh siro v qut.
- iu ch cn aconit
Rt dch chit.
nh lng alkaloid ton phn.
iu ch siro hng chanh
- iu ch nc bc h
- iu ch dung dch m eucalyptol 1%.
- iu ch siro tr ho.

13