NI DUNG THC HNH BUI 1

Th 2, 11/07/2016
1. Pha cn 90% t cn nguyn liu
Pha cn theo 4 bc
1. Xc nh nng cn nguyn liu
2. Tnh ton
3. Pha cn
4. Kim tra li
Cho cn nguyn liu vo ng ong, cho cn k ni t do trn ng ong (khng chm vo y
hay thnh ng).
c cn biu kin trn mt cht lng v nhit
N8= 97% - 29oC
Tra bng Gay Lussac: cn tht l 94,1 %
Tnh ton lng cn cn pha: C1.V1=C2.V2
V= (90x500)/94,1 = 478,2 ml
ong 475 ml cn vo ng ong, dng pipet ht 3,2 ml cn li thm vo ng. Thm nc va
500 ml. Khuy u.
Kim tra li cn va pha bng cn k.
N8= 93,5% - 30oC
Tra bng, cn tht l 89,8 %, t.
2. Lm m v ngm lnh cn u
Cn 40 g bt cn u, cho vo becher 250 ml, thm khong 50 ml cn 90% lm m bt
(thc t dng 30 ml cn 90%).
Thm t u bt u, trong 2 gi, y kn.
Chun b bnh ngm kit: Nht gn 1/3 phn nn, lt 1 lp giy lc, cn thm t.
Kim tra bnh ngm kit (c b chy hay khng, dng 10 ml cn 90% kim tra).
Sau 2 gi, cho bt u lm m vo, g nh cho dc liu nn li, ct giy lc bng chu vi
bnh ln trn.
Cho cn ngp mt dc liu, m mt t dch chit chy ra ui bt kh.
Ngm n bui 2 (sng T4), (ngm khong 48 gi).
3. Tm cn v qut
Cn 12 g v qut, cho vo erlen c nt mi, ngm tm vi 12 ml ethanol 80%, yn 8 gi.
Sau 8 gi (chiu T2), thm 80 ml nc si vo (2/3 lng nc) bng ng ong, lc u, yn
24 gi.
Sau 24 gi (chiu T3), gn dch chit (khng cn lc), ng vo chai= DC 1 ( c khong 50
ml).
Phn b trong bnh nn, tip tc cho thm 40 ml nc si, lc u, yn trong 15 gi.
Cu hi cn lu
1. Trnh by ni dung v kt qu bi pha cn?
2. Lp bng k cng vic s lm t sau bui 1 n 8 gi sng bui 2?
3. Tng lng cn dng
10 ml (kim tra bnh ngm kit) 12 ml (loi tp 50 ml (lm 3 pp nc thm
10 ml (dd
30 ml (lm m bt u)
dch v qut)
bc h
eucalyptol)
khong 280 300 ml (chit cn
10 ml Ethanol +50 ml
u)
Remington +10 ml Tween)
Tng ethanol 90% dng= 400 ml

NI DUNG THC HNH BUI 2

Th 4, 13/07/2016
1. Rt dch chit u
Sau qu trnh ngm lnh, rt dch chit tc 1 ml/pht (45-50 git)
Ly chai nh du th tch cn rt, ri cho nh git vo chai.
Lun cn ngp mt dc liu (khong 1-3 cm).
N8 rt dch chit gp 6 ln dc liu (240 ml trong 4 gi).
[N5 (gp 4 ln) rt 160 ml; N6 (gp 4,5 ln) rt 180 ml; N7 (gp 5,5 ln) rt 220 ml.]
Rt n ~ 200 ml (4/5 dch chit) th khng cn thm dung mi na.
(Lng dch cn trn s chy xung th tch, nu cha thm cn ln trn, p b cho
chy ht dch chit).
2. C dch chit 2 v ct cn thm dch chit 1 v qut
Gn dch chit 2 ra, c trc trn chn s.
Dch chit 1 em ct cn thm (cho vo bnh cu c gn sinh hn ngang), thu c 8 ml cn
thm.
Dch chit 1 cn li gp chung vi dch chit 2, tip tc c. Dch c cn 12 ml, ngui, thm
12 ml cn 90% vo, khuy u. Chuyn sang becher y kn cho vo t lnh.
Kt qu: thu c 8 ml cn thm + dch chit v qut.
3. Pha siro hng chanh (qua 2 giai on)
G1: iu ch dch chit hng chanh (bng phng php ct ko hi nc)
L hng chanh ti
27 g
Nc ct
v
Cn 27 g l hng chanh ti, ra, cho l vo bch nilon bp nh l, cho l vo bnh cu.
Thm nc khong 1/2 1/3 bnh cu (hoc nc gp 6 10 ln l, N8 thm 190 ml nc).
Cho bt vo, lp h thng.
Chng ct n khi thu c 21,5 ml dch chit (hng bng ng ong).
G2: iu ch 60 g siro hng chanh.(theo siro n pha ch ngui)
38,5 g
ng
21,5 ml
Dch chit hng chanh
Cn 38,5 g ng. Cho 21,5 ml dch chit hng chanh vo bnh nn nt mi, thm lng ng
va cn. y nt.
Lc u, cch thy nh <60oC n tan hon ton, ng chai, dn nhn.
4. Pha siro n.
Pha theo phng php siro n pha ch ngui.
Cn 320 g ng v becher cha 178 g nc (nn ong th tch th khi o t trng s t tt
hn). Khuy u v cch thy nh <60oC cho ha tan.
Lc siro qua ti vi kh sch ( si dy hng ln)
Ly khong 100 g siro, lm ngui v lnh n 20oC trong becher.
Cho vo ng ong o t trng bng t trng k. N8= 1,305 < 1,32
Cn phi thm ng iu chnh t trng.
m1.V1= m2.V2
m1=d1.V1 (d1: l t trng siro n o c)
m2=d2.V2 (d2= 1,32 l t trng cn t)
m2 m1 l lng ng cn b sung

Xc nh li tng lng siro ang c (ong bng ng ong)

V1=V2=V= 365 ml
Vy m1= 1,305.V
m2= 1,32.V
m= m2 m1 = (1,32 1,305). 365 = 5,475 g
Vy cn thm 5,5 g ng t t trng 1,32 20oC
Cn 5,5 g ng cho vo becher 100 ml.
Thm mt t siro n, cch thy, khuy u cho tan hon ton.
Sau thm mt t siro na pha long nng .
Thm ngay vo lng siro pha ch, khuy u ( trnh kt tinh li).
ra becher lm lnh n 20oC, cho vo ng ong 100 ml, o t trng
T trng N8= 1,315 20oC (vn trong khong cho php 1,315 1,333) (~1,32). T.
Cu hi cn lu
1. Vit cc cng thc siro iu ch trong bui 2?
2. V hnh m t iu ch siro hng chanh?
3. S khc bit khi iu ch siro hng chanh so vi siro n l?

NI DUNG THC HNH BUI 3

Th 2, 18/07/2016
1. Phi trn to dch chit m c v qut
Ly becher dch chit trong t lnh, em lc qua gc, p b, thu dch chit m c loi tp.
(dch chit lc qua 2 lp gc, dng nt mi p b cho ht dch chit).
Gp chung vi 8 ml cn thm (dch chit thu c 23,2 g).
Thm siro n va 40 g (thm 16,8 g siro n).
(Cho cn thm vo becher cha dch chit, ly chai ng cn thm lc ny em cn, chnh v
0, cho dch chit trong becher vo chai. Dng siro n trng becher ri thm vo chai ng cho
40 g).
N5= thm
g siro n
N6= thm
g siro n
N7= thm 18,2 g siro n
N8= thm 16,8 g siro n
2. Xc nh gii hn alkaloid trong cn u
Cn 2 chn dch chit cn u, mi chn 20 g, un cch thy n cn kh.
Cho 1 ml HCl 10% vo khuy tht k vi cn ( acid ha cn).
Thm 20 ml nc ct.
Thm thuc th Mayer vo mu A (4,5 ml) v mu B (5,5 ml).
Lc ring tng mu qua giy lc (kh, khng thm nc) cho vo becher.
Cho vo mi mu 1 ml thc th Mayer, lc u v c kt qu lin. Ghi nhn v gii thch kt
qu. (bng cch so snh).
Rt ra kt lun: t l dch chit so vi dc liu bao nhiu th t.
Kt qu:
N5= 160 ml (gp 4 ln DL)
N6= 180 ml (gp 4,5 ln DL)
N7= 220 ml (gp 5,5 ln DL)
N8= 240 ml (gp 6 ln DL)
Kt lun: Vy 40 g dc liu u, th dch chit cn rt 4 5 ln dc liu th t.
3. iu ch nc thm bc h
Theo 3 phng php:
Ethanol lm trung gian ha tan
Giai on 1: Cn bc h 1%
Tinh du bc h
0,1 g
Ethanol 90 % v
10 g
Cn 0,1 g tinh du bc h vo bnh nn kh sch, thm ethanol 90% v 10 g. Lc u.
Giai on 2: Nc thm bc h
Cn bc h 1%
1,5 g
Nc ct v
50 g
Cn 1,5 g cn bc h 1% vo bnh nn kh sch, thm nc ct v 50 g. Lc u.
Lc qua giy lc c thm nc. Ghi nhn hin tng.

Remington
Giai on 1: iu ch nc thm m c (50 ml)
Tinh du bc h
1 ml
Ethanol 90 %
30 ml
Nc ct v
50 ml
Bt Talc
2,5 g
Chun b 1 erlen c nt mi sch kh c nh du sn mc 50 ml.
Cho 1 ml tinh du bc h v ong 30 ml ethanol 90 % vo erlen trn y np lc u.
Thm t t nc ct va 50 ml, lc mnh.
Trn 2,5 g bt Talc vo hn hp trn, lc u sau yn 1 gi.
Lc qua giy lc c thm nc.
Giai on 2: iu ch nc thm bc h (50 ml)
Nc thm m c 1,25 ml
Nc ct
48,75 ml
Ly 1,25 ml nc thm m c vo erlen c nt mi.
Thm nc ct 48,75 ml n vch vch sn. Lc u.
Tween lm trung gian ha tan
Tinh du bc h
0,1 g
Tween 20
1g
Ethanol 90%
10 g
Nc ct v
50 g
Cn 0,1 g tinh du bc h v 1 g Tween 20 cho vo erlen c nt mi, y np v lc u.
Cn 10 g ethanol 90% vo erlen trn lc u, thm nc ct va 50 g lc u tip.
Lc qua giy lc thm nc. ng chai, dn nhn, bo qun.
4. Th nghim vi acid citric v natri benzoate
Th nghim 1:
Ha tan ln lt 0,4 g natri benzoate v 0,4 g acid citric vo 8 ml nc.
Th nghim 2:
Ha tan 0,4 g natri benzoate vo 4 ml nc (1).
Ha tan 0,4 g acid citric vo 4 ml nc (2).
Chun b khong 200 ml nc. Thm ln lt (1) v (2) vo. Khuy u.
Kt lun:
Khng th ha tan chung acid citric v natri benzoate vo lng dung mi qu nh (s to ta).
Nn ha tan ring ri phi hp vo 1 lng (t dc) ln. Khi phi hp 2 cht vo 1 dung dch
long th s khng ta).
Cu hi cn lu
1. Nn ha tan acid citric vo thnh phn no?
2. C cn thit phi chia i lng nc ha tan acid citric v natri benzoate khng? Chia cch khc
c khng

NI DUNG THC HNH BUI 4

Th 4, 20/07/2016
1. Pha siro v qut
Dch chit m c v qut (40 g) + 9 phn siro n (360 g)
Phi trn theo kl/kl
Cn li siro n v dch chit m c
Cn 360 g siro n v 40 g dch chit m c, trn vi nhau, trn bercher dch chit, khuy u
2. Pha dung dch Eucalyptol 10% (Pha dung dch m)
Pha 10 g dung dch Eucalyptol 10% (kl/kl)
Eucalyptol
1g
Ethanol 90%
v 10 g
Chun b erlen kh sch, t erlen ln cn, chnh v 0. Cn 1 g Eucalyptol v erlen.
Thm ethanol 90% va 10 g, lc u. ng l, dn nhn.
3. Pha dung dch Bromoform 10%
Pha 20 g dung dch Bromoform dc dng 10%
Pha 100 g Pha 20 g
Bromoform
10 g
2g
Glycerin dc dng
30 g
6g
Ethanol 90%
60 g
12 g
iu ch theo phng php ha tan
Cn 6 g glycerin dc dng v 12 g ethanol 90% vo chai c nt mi, lc u.
Cho tip 2 g Bromoform vo hn hp trn, lc cho tan hon ton.
Lc, ng chai, dn nhn.
4. iu ch 4 n v siro tr ho
Dn d:
Khng pha chung acid citric v natri benzoate trong 1 th tch nh
Cc dung dch cn phi c pha chung vi nhau (do dch chit cn gp nc c kh nng ta).
Phi phi t t dung dch cn vo lng ln siro c trong cng thc
Cn aconite c cha alkaloid, nn d b ta khi gp mi trng nc. Cn mi trng c pH
acid trnh b ta. Acidcitric ng vai tr iu chnh pH trnh alkaloid b ta.
Cn thc cho 4 n v siro ho thnh phm
Thnh phn
4 n v sn phm
Dung dch Bromoform dc dng 10%
2,4 g
Cn Aconite
2,4 g
Dung dch Eucalyptol 10% (kl/kl)
0,4 g
Siro hng chanh
48 g
Nc bc h
20 ml
Acid citric
0,4 g
Natri benzoate
0,4 g
Nc tinh khit
8 ml
Siro v qut (v)
400 g

Cch pha ch 4 n v siro tr ho (d kin 1)

- Cn 0,4 g natri benzoate vo becher 50 ml, thm 4 ml nc ct, khuy u n tan hon ton
(1).
- Cn 0,4 g acid citric vo becher 50 ml, thm 4 ml nc ct, khuy u n tan hon ton (2).
- Cho 2,4 g dd Bromoform 10%, 2,4 g cn aconite, 0,4 g dung dch eucalyptol 10% vo becher
50 ml sch kh, khuy u n ng nht, thu c hn hp cn thuc (3).
- Cho 200 g siro v qut vo becher 1000 ml sch v kh, cho dd (2) vo khuy u, sau cho
t t hn hp cn thuc (3) vo, khuy u.
- Tip tc cho t t 48 g siro hng chanh vo becher, khuy u.
- Sau , cho dung dch natri benzoat (1) vo becher, khuy u v k.
- Cui cng cho 20 ml nc bc h vo, khuy u
- t mt becher 1000 ml khc, sch kh ln cn, chnh v 0. hn hp mi pha vo, dng siro
v qut trng tt c cc becher, thm siro v qut cho vo va 400 g, khuy u n khi hn
hp ng nht.
- Chia u vo 4 chai sch, mi chai 100 g, ng np dn nhn.
Cch pha ch 4 n v siro tr ho (d kin 2)
- Cn 0,4 g natri benzoate vo becher 50 ml, thm 8 ml nc ct, khuy u n tan hon ton
(1).
- Cho 2,4 g dd Bromoform 10%, 2,4 g cn aconite, 0,4 g dung dch eucalyptol 10% vo becher
50 ml sch kh, khuy u n ng nht. Thm 0,4 g acid citric vo hn hp trn, khuy tht
u cho tan ht, thu c hn hp cn thuc (3).
- Cho 200 g siro v qut vo becher 1000 ml sch v kh, sau cho t t hn hp cn thuc
(3) vo, khuy u v k n ng nht.
- Tip tc cho t t 48 g siro hng chanh vo becher, khuy u.
- Sau , cho dung dch natri benzoat (1) vo becher, khuy u v k.
- Cui cng cho 20 ml nc bc h vo, khuy u.
- t mt becher 1000 ml khc, sch kh ln cn, chnh v 0. hn hp mi pha vo, dng siro
v qut trng tt c cc becher, thm siro v qut cho vo va 400 g, khuy u n khi hn
hp ng nht.
- Chia u vo 4 chai sch, mi chai 100 g, ng np dn nhn.
Cu hi cn lu

NI DUNG CHI TIT THC HIN

1. Pha 500 ml cn 90 t cn nguyn liu
2. iu ch Cn u
3. iu ch dch chit m c v qut
3. iu ch siro v qut
4. iu ch siro hng chanh
5. iu ch siro n
6. iu ch nc thm Bc h

TRNG I HC Y DC TP.HCM
KHOA DC - B MN BO CH
41- 43 inh Tin Hong, P. Bn Ngh, Q.1, TP.HCM

TRNG I HC Y DC TP.HCM
KHOA DC - B MN BO CH
41- 43 inh Tin Hong, P. Bn Ngh, Q.1, TP.HCM

SIRO TR HO

SIRO TR HO

Chai 100 g
Cn Aconit....................................................................0,06 g
Eucalyptol.....................................................................0,01 g
Siro hng chanh...............................................................12 g
Nc bc h.....................................................................5 ml
Siro v qut v t dc (v)............................................100g
S l SX: 0716
SK: VD072016
Ngy pha ch: 20/07/2016
Ch nh: Tr ho
HSD: 20/07/2017
Bo qun: xem t HDSD
xa tm tay tr em, c k hng dn trc khi s dng

Chai 100 g
Cn Aconit....................................................................0,06 g
Eucalyptol.....................................................................0,01 g
Siro hng chanh...............................................................12 g
Nc bc h.....................................................................5 ml
Siro v qut v t dc (v)............................................100g
S l SX: 0716
SK: VD072016
Ngy pha ch: 20/07/2016
Ch nh: Tr ho
HSD: 20/07/2017
Bo qun: xem t HDSD
xa tm tay tr em, c k hng dn trc khi s dng

TRNG I HC Y DC TP.HCM
KHOA DC - B MN BO CH
41- 43 inh Tin Hong, P. Bn Ngh, Q.1, TP.HCM

TRNG I HC Y DC TP.HCM
KHOA DC - B MN BO CH
41- 43 inh Tin Hong, P. Bn Ngh, Q.1, TP.HCM

SIRO TR HO

SIRO TR HO

Chai 100 g
Cn Aconit....................................................................0,06 g
Eucalyptol.....................................................................0,01 g
Siro hng chanh...............................................................12 g
Nc bc h.....................................................................5 ml
Siro v qut v t dc (v)............................................100g
S l SX: 0716
SK: VD072016
Ngy pha ch: 20/07/2016
Ch nh: Tr ho
HSD: 20/07/2017
Bo qun: xem t HDSD
xa tm tay tr em, c k hng dn trc khi s dng

Chai 100 g
Cn Aconit....................................................................0,06 g
Eucalyptol.....................................................................0,01 g
Siro hng chanh...............................................................12 g
Nc bc h.....................................................................5 ml
Siro v qut v t dc (v)............................................100g
S l SX: 0716
SK: VD072016
Ngy pha ch: 20/07/2016
Ch nh: Tr ho
HSD: 20/07/2017
Bo qun: xem t HDSD
xa tm tay tr em, c k hng dn trc khi s dng

TRNG I HC Y DC TP.HCM
KHOA DC - B MN BO CH
41- 43 inh Tin Hong, P. Bn Ngh, Q.1, TP.HCM

TRNG I HC Y DC TP.HCM
KHOA DC - B MN BO CH
41- 43 inh Tin Hong, P. Bn Ngh, Q.1, TP.HCM

SIRO TR HO

SIRO TR HO

Chai 100 g
Cn Aconit....................................................................0,06 g
Eucalyptol.....................................................................0,01 g
Siro hng chanh...............................................................12 g
Nc bc h.....................................................................5 ml
Siro v qut v t dc (v)............................................100g
S l SX: 0716
SK: VD072016
Ngy pha ch: 20/07/2016
Ch nh: Tr ho
HSD: 20/07/2017
Bo qun: xem t HDSD
xa tm tay tr em, c k hng dn trc khi s dng

Chai 100 g
Cn Aconit....................................................................0,06 g
Eucalyptol.....................................................................0,01 g
Siro hng chanh...............................................................12 g
Nc bc h.....................................................................5 ml
Siro v qut v t dc (v)............................................100g
S l SX: 0716
SK: VD072016
Ngy pha ch: 20/07/2016
Ch nh: Tr ho
HSD: 20/07/2017
Bo qun: xem t HDSD
xa tm tay tr em, c k hng dn trc khi s dng