Principles of Biochemistry (Mid Term Paper)

(1) The pH of the 0.1M Hcl solution will be

(A) 0 (B) 1 (C) 2 (D) 3 (E) 7
(2 ) In glucose the orientation of H and OH groups around the carbon atom 5 adjacent to the terminal
primary alcohol carbon determines
(A) D & L series (B) Dextro or Levorotatory (C) Anomers (D) Epimers (E) Isomers
(3) The maximum numbers of isomers of glucose are
(A) 2 (B) 4 (C) 8 (D) 16 (E) 32
(4) Two sugars which differ from one another only in configuration around a single carbon atom are
termed
(A) Epimers (B) Anomers (C) optical isomers (D) sterioisomers (E) Enantiomers
(5) Isomers differing as a result of variation in configuration of the H & OH on carbon atoms 2, 3 & 4
of glucose are known as
(A) Epimers (B) Anomers (C) optical isomers (D) sterioisomers (E) D & L series
(6) A carbohydrate commonly known as dextrose is
(A) Dextrin (B) D-Fructose (C) D-Glucose (D) Glycogen (E) Starch
(7) Which of the following is an Epimers pair?
(A) Glucose & Fructose (B) Glucose & Galactose (C) Galactose & Mannose (D) Lactose &
Maltose (E) D-Glucose & L-Gluctose
(8) On boiling Benedict`s solution is not reduced by
(A) Sucrose (B) Lactose (C) Maltose (D) Fructose (E) Glucose
(9) Sugar phosphate is formed by process of
(A) Oxidation (B) Reduction (C) phosphorisation (D) Esteriffication (E) Hydration
(10) In amylopectin the interval of glucose units of each branch is
(A) 10-20 (B) 24-30 (C) 30-40 (D) 40-50 (E) 50-60
(11) Amylose is a constituent of
(A) Starch (B) Cellulose (C) Glycogen (D) Maltose (E) None of these
(12) The pH of blood is 7.4, the ratio between carbonic acid and bicarbonate fraction is
(A) 1:10 (B) 1:20 (C) 1:30 (D) 1:40 (E) 1:1

(13) Within the Red Blood Cells the buffering capacity contributed by the phosphate is
(A) 5% (B) 7% (C) 20% (D) 35% (E) 53%

(14) Osmotic pressure of plasma is about

(A) 10 mm of Hg (B) 15 mm of Hg (C) 25 mm of Hg (D) 50 mm of Hg (E) 100 mm of Hg
(15) The Glycoaminoglycan which does not contain protein is
(A) Dermatan Sulphate (B) Chondroitin Sulphate (C) Keratin Sulphate (D) Heparan Sulphate
(E) Hyaluronic acid
(16) Repating units of Hyaluronic acid are
(A) N-acetyl glucosamine & D-glucuronic acid (B) N-acetyl galactose & D-glucuronic acid
(C) N-acetyl glucosamine & galactose (D) N-acetyl galactosamine & iduronic acid
(17) The stable Ring formation in D-glucose involves
(A) C-1 & C-4

(B) C-1 & C-2

(C) C-1 & C-5

(D) C-2 & C-5 (E) C-1 & C-6

(18) Wavelength of sodium light for measuring optical activity of sugar is

(A) 520 nm

(B) 549 nm

(C) 579 nm

(D) 589 nm

(E) 599 nm

Q#2 Define the term Mutarotation, explain the phenomenon with freshly prepared solution of alpha
D-Glucose.
(1+2)
Q#3 What are Glycosides,give their types and uses?

(1+1+1)

Q#4 What are Buffers. How they work & give the %age of any three buffers found in the body?
(1+1+1)
Q#5 0.22 M solution of Acetic acid (pka 4.7) was found to contain 0.20 M in the dissociated form
and 0.020 M un-dissociated form, Find out the pH of the solution?
(1+1+1)
OR
Q#1 What is Optical activity and give formula of specific Ratio?
Q#2Write down the Different ways to cross the materials across the cell membrane?
Q#3 What are Buffers, Enantiomers and Glucurone?