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Arteries carry blood away from the heart. They are classified into three types according to their
size: large or elastic arteries; medium (or muscular or distributive) arteries; and small arteries or
arterioles, which are less than 0.5 mm in diameter. These types are all continuous with one
another. A characteristic feature of arteries, regardless of size, is a well-defined lumen, rounded
or oval, maintained by the muscularity of the vessel wall.
Large Arteries:
The aorta and its branches (brachiocephalic, subclavian, pulmonary, beginning of common
carotid and iliac) are distinguished by their great elasticity. This helps them smooth out the large
fluctuations in blood pressure created by the heartbeat. During systole, their elastic laminae are
stretched and reduce blood pressure. During diastole, the elastic rebound helps maintain arterial
pressure.
Tunica intima: Large arteries often have a large subendothelial layer, which grows with age or
disease conditions (arteriosclerosis). Both connective tissue and smooth muscle are present in the
intima. The border of the intima is delineated by the internal elastic membrane. The internal
elastic membrane may not be conspicuous because of the abundance of elastic material in the
tunica media.
Tunica media: This is the thickest of the three layers. The smooth muscle cells are arranged in a
spiral around the long axis of the vessel. They secrete elastin in the form of sheets, or lamellae,
which are fenestrated to facilitate diffusion. The number of lamellae increase with age (few at
birth, 40-70 in adult) and with hypertension. These lamellae, and the large size of the media, are
the most striking histological feature of elastic arteries. In addition to elastin, the smooth muscle
cells of the media secrete reticular and fine collagen fibers and proteoglycans (all not
identifiable). No fibroblasts are present.
Tunica adventitia: This is a relatively thin connective tissue layer. Fibroblasts arethe predominant
cell type, and many macrophages are also present. Collagen fibres predominate and elastic fibres
(not lamellae) are also present. The collagen in the adventitia prevents elastic arteries from
stretching beyond their physiological limits during systole. Blood vessels supplying the
adventitia and outer media are also present, these are called vasa vasorum ("vessels of the
vessels"). (The inner part of the media is supplied from the lumen via pinocytic transport).
Figure 11 shows a low power view of the aorta (from slide 29).
The thickness of the tunica media and the abundance of wavy red
elastic lamellae is evident. Elastin is also present in the (partially
detached) tunica intima but the endothelium cannot be
distinguished. An arteriole (vasa vasorum) is present in the
adventitia. The big red lines running across the aorta are artefacts
produced where the tissue folded during preparation.
Medium Arteries:
The majority of named arteries are medium (muscular or distributive) arteries. There is no sharp
dividing line between elastic (large) and muscular (medium) arteries; in areas of transition,
arteries may appear as intermediates between the two types. Medium arteries have less elastic
tissue than large arteries, the predominant constituent of the tunica media is smooth muscle.
Tunica intima: The tunica intima is thinner than in large arteries, there are fewer smooth muscle
cells and less elastic tissue. The outermost part of the intima is defined by a very prominent
internal elastic membrane (not obscured by elastic lamellae as in large arteries). The basement
membrane of the endothelium may rest directly on the internal elastic membrane, or be separated
by a subendothelial layer of CT. The tunica intima increases in thickness with age, and may also
become expanded by lipid deposits.
Tunica media: Smooth muscle cells predominate in the tunica media, and little elastic material is
present. As in large arteries, no fibroblasts are present. Elastic fibres (few), collagen, and ground
substance are produced by the smooth muscle cells. These are arranged in a spiral fashion and
their contraction helps maintain blood pressure. In tissue preparation, the internal elastic
membrane of the intima appears wavy due to the contraction of the smooth muscle of the media.
Tunica adventitia: The main constituent of the adventitia is collagen fibres, secreted by
fibroblasts. Elastic fibres are also present, a concentration of such fibres at the inner boundary of
the adventitia is called the external elastic membrane. The external elastic membrane is not as
prominent as the internal, and as arteries get smaller (see small arteries, below) disappears much
earlier. The tunica adventita is relatively larger than in elastic arteries, it can be up to the same
size as the media. It will often blend in with the CT of surrounding structures. Adipose cells may
be present.
Small Arteries:
Different textbooks will give slightly different numbers for what constitutes the diameter of
small arteries and arterioles. Again there is a gradation to larger vessels. The general construction
of small arteries is very similar to that of muscular arteries. The media is still muscular and has
up to 8-10 layers of smooth muscle cells. This number is reduced as the arteries get smaller, the
smallest arterioles have 1-2 layers of smooth muscle cells. The adventitia becomes thinner and
the external elastic membrane disappears. The intima becomes smaller and the internal elastic
membrane also eventually disappears. However, it persist much longer than the external, and it is
not uncommon to see very small arteries which still have an internal elastic membrane. Small
arteries also maintain their shape, and tend to be round or oval.
Veins:
Veins are the vessels that return blood to the heart. Like arteries, they are classified as large,
medium and small, and the sizes blend into one another with no sharp demarcations. Although
the same layers (intima, media and adventitia) are present, they are often not as well defined as
in arteries. A big difference between arteries and veins is the thickness of their walls and the
relative amount of muscle tissue (media). In comparably sized vessels, arteries have thicker walls
and a much larger media. In veins, the adventitia is larger than the media. Because of these
features, veins do not retain their shape. They often appear floppy in sections, and the lumen may
not be patent. Veins are frequently of an irregular shape. Veins also have less elastic tissue than
do arteries. Even in larger veins, the internal elastic membrane may be poorly developed or
absent. (It may also be present, so dont assume something is an artery just because you see
elastic tissue.) Valves, which function to prevent the backflow of blood especially in the lower
part of the body, are also seen quite frequently in veins. Veins often travel in close proximity to
their arterial counterparts, which is convenient for histological comparison.
Large veins:
The large veins are the venae cavae and portal vein and their tributaries.
The tunica intima consists of the endothelial lining with its basement membrane, a small amount
of subendothelial connective tissue and some smooth muscle cells. It blends in with the tunica
media which is relatively thin, and in addition to smooth muscle cells may contain collagen
fibres and some fibroblasts (in contrast with the media of arteries). The most distinguishing
feature of large veins is the large tunica adventitia. The adventitia is the thickest layer in large
veins and is made of collagen fibres, some elastic fibres and fibroblasts. Prominent bundles of
longitudinally-arranged smooth muscle are a distinguishing feature.
Medium Veins:
The tunica consists of the endothelium and a thin subendothelial layer with smooth muscle cells
among the connective tissue elements. A thin internal elastic membrane may or may not be
present. (If present, it is not nearly as prominent as in arteries).
The tunica media is much thinner relative to that of an artery, and consists mostly of circularly
arranged smooth muscle but also contains collagen fibres. The tunicas intima and media
therefore tend to be less distinct from one another than is the case in arteries.
The tunica adventitia is usually thicker than the media and is made up mostly of collagen fibres.
It may contain longitudinally oriented smooth muscle bundles. (Remember gradations between
the vessels of different sizes are continuous.)
Figure 20 shows a high power view of the top part of the vein and
the valve seen in Figure 19. A few endothelial cell nuclei can be
seen but the intima is not distinct. The valve is a fold of the tunica
intima reinforced with connective tissue. The tunica media and
tunica adventitia are about the same size, the latter blends merges
with the surrounding connective tissue (which in the field of view
is adipose tissue). Some coagulated blood can be seen adhering to
the right side of the valve, and dispersed to the left of it. Some
individual, mainly white, blood cells can be seen at the top.
vasoactive agents such as histamine and serotonin which cause extravasation of fluid and WBCs
during inflammation or allergic reactions.
Collecting venules have a thin adventitia in addition to the pericytes surrounding the intima. The
adventitia consists of of longitudinally arranged collagen fibres with a few elastin fibres.
Muscular venules have 1-3 layers of smooth muscle surrounding the intima, and an adventitia as
described above.
When looking at a venule which appears to have a media, it is generally not possible to tell if the
nuclei of the media belong to smooth muscle cells or pericytes.
Capillaries:
Capillaries are the smallest diameter vessels and the site of exchange of metabolites between
blood and tissues. Capillaries are just wide enough to allow the passage of red blood cells, only
one cell at a time. (It is during this squeezing through the capillaries that mis-shapen RBCs, as in
sickle cell anemia and thalassemia, rupture.)
Capillaries consist of a single layer of endothelial cells and their basement membrane. The
endothelial cells are joined together by tight junctions. At intervals, these tight junctions are
interrupted, leaving small spaces allowing the passage of fluid between blood and ECF. These
interruptions do not occur in the brain, and the lack thereof is responsible for the blood-brain
barrier present in most of the brain. Endothelial cells also have pinocytotic vesicles which are
involved in transporting macromolecules.
Capillaries are classified according to the structure of their endothelial cells.
1. Continuous capillaries (most capillaries) have a continuous endothelial cells with no
fenestrations (openings) in their walls. They are found in nervous tissue, muscle tissue,
lung, connective tissue and exocrine glands.
2. a. Fenestrated capillaries have endothelial cells in which are found small openings, called
fenestrae, of about 80- 100 nm in diameter. The fenestrations are covered by a small nonmembranous diaphragm (which may be the remnant of the glycocalyx enclosed by
pinocytotic vesicles from which the fenestrae may be formed). The basement membrane
of endothelial cells is continuous over the fenestrae. Fenestrae allow greater permeability
and the rapid passage of macromolecules smaller than plasma proteins. Fenestrated
capillaries are found in the intestine and endocrine glands.
b. A special type of fenestrated capillary with no diaphragm is found in the renal
glomerulus. This capillary has a thick basement membrane.
3. Sinusoids, also called discontinuous capillaries, have a large lumen and follow a tortuous
path. They have many fenestrations with no diaphragm , and a discontinuous or absent
basal lamina. The lumen is lined with phagocytic cells. They are found in the liver,
hematopoietic organs (bone marrow, spleen) and some endocrine organs.
At intervals, the endothelial cells of capillaries may be surrounded by pericytes, undifferentiated
mesenchyme cells (as in post-capillary venules). Pericytes can differentiate into fibroblasts and
smooth muscle cell, and function in wound repair and the formation of new vessels. Pericytes are
associated much more frequently with continuous than with fenestrated capillaries.
It is not possible to distinguish different types of capillaries (except sinusoids) with the light
microscope. They are recognized by their small (1 RBC) diameter and thin wall.