Quy trnh tch DNA tng s ca vi khun

Bc 1. Nui sinh khi.

Bc 2. Ly tm dn: 7000 vng trong 4 pht nhit phng.
Bc 3. Ti huyn ph bng TE: 740l.
Bc 4. Thm SDS 10%: 40l (votex mnh).
Bc 5. Thm proteinase K (10mg/ml): 8l (votex mnh).
Bc 6. 560C trong 2 gi.
Bc 7. Thm NaCl 5M: 100l (votex mnh).
Bc 8. Thm CTAB/NaCl (un nng n 650C): 100l (votex mnh).
Bc 9 650C trong 10 pht bng my Water baths.
Bc 10. Thm phenol: chloroform: isoamyl alcohol (25:24:1): 0,5ml.
Bc 11. Ly tm 13.000 vng trong 10 pht nhit phng. Ly dch ni.
Bc 12. Thm isopropanol (-20): 324l.
Bc 13. -200C trong 2 gi
Bc 14. Ly tm 13.000 vng trong 15 pht nhit thng.
Bc 15. Ra bng EtOH 70% (-200C).
Bc 16. B phn ni v mu kh nhit phng trong 1 gi.
Bc 17. Ha tan mu bng 20l nc ct v trng.