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Titanium dioxide-based antibacterial surfaces
for water treatment
Changseok Han1, Jacob Lalley2, Devi Namboodiri3,
Keeley Cromer2 and Mallikarjuna N Nadagouda2
The field of water disinfection has gained much interest since
waterborne diseases caused by pathogenic microorganisms
directly endanger human health. Antibacterial surfaces offer a
new, ecofriendly technique to reduce the harmful disinfection
byproducts that form in medical and food processing
industries. Titanium dioxide (TiO2) photocatalysts have been
extensively studied to prepare antibacterial surfaces due to
their environmentally favorable properties. The studies
demonstrate TiO2 improves the efficiency of disinfection by the
effective inactivation of pathogenic microorganisms (i.e.,
Escherichia coli, Enterococcus faecalis, Staphylococcus
aureus, Pseudomonas putida, and Listeria innocua). TiO2
photocatalysts decompose natural algal toxins such as
microcystin-LR and cylindrospermopsin under solar light
irradiation. On the basis of literature review, these antibacterial
surfaces may be applied to hospital, food, ceramic, and
building industries or to environmental remediation where
bacteria inactivation is required to ensure the safety of human
health and the environment.
Addresses
1
ORISE Post-doctoral Fellow, The U.S. Environmental Protection
Agency, ORD, NRMRL, STD, CPB, 26 W. Martin Luther King Jr. Drive,
Cincinnati, OH 45268, USA
2
The U.S. Environmental Protection Agency, ORD, NRMRL, WSWRD,
WQMB, 26 W. Martin Luther King Jr. Drive, Cincinnati, OH 45268, USA
3
Seven Hills High School, 5400 Red Bank Road, Cincinnati, OH 45227,
USA
Corresponding author: Nadagouda, Mallikarjuna N
(nadagouda.mallikarjuna@epa.gov)
http://dx.doi.org/10.1016/j.coche.2015.11.007
2211-3398/Published by Elsevier Ltd.
Introduction
Water disinfection is a significant process for drinking
water treatment since it is directly related to the reduction
of waterborne diseases caused by pathogenic microorganisms [13]. Chlorination is a common technique used for
water disinfection to inactivate or kill pathogens in water
Current Opinion in Chemical Engineering 2016, 11:4651
treatment plants. However, there are several disadvantages. During chlorination, harmful compounds known as
disinfection byproducts (DBPs) are produced due to a
reaction of chlorine and natural organic matter (NOM) in
water. DBPs contain carcinogens (i.e., trihalomethanes
(THMs) and haloacetic acids (HAAs)) and are associated
with bladder concerns that may contribute to adverse
productive effects [4,5]. Moreover, it has been reported
that after chlorination, multidrug resistant (MDR) pathogens (i.e., Acinetobacter baumannii, and Pseudomonas aeruginosa) grow and survive [5,68]. Although new,
alternative techniques, such as UV-disinfection and ozonation, have been studied and employed in water treatment plants to solve these problems, complications
regarding residual of pathogens have still been reported
[5]. Therefore, there is a need to develop a reliable
method of disinfection, which is free from the production
of DBPs and effective at killing or inactivating pathogens.
Also, further studies are required to ensure that new
techniques do not have adverse effects to the safety of
human health and the environment.
One alternative approach of disinfection is utilizing antibacterial surfaces. Antibacterial surfaces offer a different
technique compared to conventional disinfection since
water-containing pathogens require contact with the antibacterial surfaces to kill or inactivate pathogens as
opposed to chemicals (e.g., chlorine, chloramines, and
chlorine dioxide) that are directly dispersed into water
[5]. Hitherto, antibacterial surfaces have been used in
medical fields to prevent infection and the formation of
biofilms on the surface of medical devices and implants
[5,9]. Recently, the antibacterial surfaces prepared on
different substrates, such as stainless steel, glass, and
ceramics, have been used for water treatment [10].
Because of its antibacterial activity, titanium dioxide
(TiO2) has been extensively studied and used to inactivate
many microorganisms [11,12,13]. Also, being environmentally suitable, it has been widely used for environmental applications, including water and air purification as well
as groundwater and soil remediation [14,15,16,17].
Therefore, in this review, we focus on the antibacterial
surfaces of TiO2 for drinking water disinfection.
Titanium dioxide
TiO2 is the most commonly used semiconducting photocatalyst and one of the most studied nanoparticles for
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environmental applications. Upon UV irradiation, activated TiO2 yields photocatalytic properties applicable for
water purification techniques. Photocatalytically generated reactive oxygen species (ROS), such as hydroxyl
radicals (HO), hydrogen peroxide (H2O2), and superoxide anion (O2 ) can decompose inorganic and organic
pollutants and inactivate microorganisms, bacteria, fungi,
and viruses [11,18]. Figure 1 shows the photoexcitation
mechanism of TiO2 in detail. Economic and environmental factors make TiO2 disinfection a viable replacement
for chlorination. For example, TiO2 catalysts can be
recycled indefinitely, requiring no additional chemical
treatment, thus making its large-scale use more probable
[18].
Since photocatalysis is an interfacial phenomenon, disinfection will be optimized when the active surface-area-tovolume ratio is maximized. Therefore, maximum disinfection would occur with a mobilized mode of TiO2 (a
slurry of nanosized TiO2 powder suspended in a mixture).
The mobilized mode causes the separation between fine
TiO2 powders and the disinfected water to be a difficult
process that requires additional steps and equipment. To
accommodate for this problem, an immobilized mode for
TiO2 catalysis, though not as efficient at disinfection, is
the logical option. Immobilized TiO2 permanently
adheres to supporting materials such as soda-lime glass
and acrylic bottles.
The solar disinfection method (SODIS) was one technique explored and modified by the addition of a photocatalytic layer of TiO2 (using solvent deposition) on the
interior surface of polyethylene terephthalate (PET) and
acrylic bottles [19]. It was determined that the TiO2 coated
acrylic bottles could effectively inactivate and transform
bacteria (e.g., Escherichia coli (E. coli), organic waste (e.g.,
methyl orange), and algal toxins (e.g., microcystin-LR
(MC-LR)) after various times of solar irradiation. The
TiO2 coated acrylic bottles were found to be an effective
alternative to TiO2 coated PET bottles due to acrylics
greater UV transparency [19]. Thus, plastic bottles coated
with TiO2 and irradiated by sunlight can effectively kill
common waterborne pathogenic microorganisms and other organic pollutants, proving to be beneficial to areas with
inadequate amounts of clean water. Gelover et al. [20]
immobilized TiO2 on glass cylinders by the solgel method [21] to enhance the efficiency of SODIS. The TiO2
coated glass cylinders were placed in a homemade solar
collector (Figure 2). The collector was then exposed to
solar light with an average radiation of 1037 W m 2. With
the TiO2 coating, complete inactivation of E. coli without
bacteria regrowth was obtained after 15 min of solar irradiation. However, for SODIS without TiO2, regrowth
occurred and it took 30 min to inactivate E. coli.
Bonetta et al. [22] and Alrousan et al. [23] studied the
inactivation of pathogens by immobilizing TiO2 on petri
Figure 1
Ox
Conduction Band
Ox
Substrate
Reduction
UV or visible light
Bandgap (3.2 or < 3.2eV)
h+
Valence Band
Red
+
Oxidation
Red+
Immobilized TiO2
Current Opinion in Chemical Engineering
48 Materials engineering
Figure 2
60
19
Current Opinion in Chemical Engineering
UV and visible light for photocatalytic bacterial inactivation. Because of the necessity of light, research in metal or
nonmetal-doped TiO2 compounds (e.g., silver and copper-doped TiO2, and nitrogen-doped TiO2) has been
conducted. Results showed antibacterial activity occurring with limited or no light irradiation in both cases
[23,29,24,3042]. In addition, the use of Ag enhanced
TiO2 disinfection is considerably interesting. Investigations have been performed on the use of Ag enhanced
TiO2 surfaces and their wide range of potential uses from
the photodegradation of estrogen to E. coli inactivation
[29,24,3042].
Moreover, a significant drawback of TiO2 photocatalysis
is the rapid charge recombination of the electronhole
pairs, which reduces the efficiency of the photocatalytic
processes, for its practical applications. Therefore, the
improvement of charge separation during the TiO2
photocatalysis became a significant strategy to enhance
the efficiency of the process. The formation of heterojunction of TiO2 with other semiconductors and/or noble
metals has been widely studied to achieve high photocatalytic efficiency of the processes by decreasing the
recombination rate of photoinduced electronhole pair
[43].
Conclusion
The field of water disinfection is gaining interest with
immobilized bactericidal agents on surfaces. Though
used for years in medical and food processing industries,
antibacterial surfaces are now considered a new and
environmentally efficient technique for water disinfection to reduce the amount of carcinogenic byproducts
formed through chlorine and NOM reactions. ROS produced at the surfaces composed of TiO2 demonstrate
reliable antibacterial properties to inactivate various pathogenic microorganisms such as E. coli, Enterococcus faecalis,
Staphylococcus aureus, Pseudomonas putida, and Listeria
innocua. Moreover, algal toxins were effectively decomposed by TiO2 photocatalysis. Therefore, these antibacterial surfaces may be used in many applications,
including hospital and food industry, ceramic and building industry, and environmental remediation.
Disclosure
The U.S. Environmental Protection Agency, through its
Office of Research and Development, funded and managed, or partially funded and collaborated in, the research
described herein. It has been subjected to the Agencys
administrative review and has been approved for external
publication. Any opinions expressed in this paper are
those of the author(s) and do not necessarily reflect the
views of the Agency, therefore, no official endorsement
should be inferred. Any mention of trade names or
commercial products does not constitute endorsement
or recommendation for use.
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