350

375
400
425
450
475
500
525
550
575
600
625
650
675
700
725
750
775
800

0.025
0.019
0.04
0.097
0.22
0.448
0.819
0.933
0.374
0.063
0.014
0.006
0.004
0.004
0.005
0.003
0.004
0.003
0.003

475
485
495
505
515
525

0.462
0.619
0.764
0.881
0.95
0.933

Optimum

10
5
3.33
2.5
2
1.67
1.43
1.25
1.11
1

0.95
0.495
0.342
0.24
0.225
0.182
0.175
0.171
0.141
0.121

10
5
3.33
2.5
2
1.67
1.43

11.2
32
45.5
57.5
59.6
65.8
66.9

Calibratio

f(x) = 0.0

Calibration

f(x) = 91.78

Calibration
1.25
1.11
1

67.4
72.3
75.7

f(x) = 91.78

Optimum Wavelength Determination

Wavelength Determination

Calibration Curve: Concentration vs. Absorbance

f(x) = 0.0910606418x + 0.0374833802

Calibration Curve: Concentration vs. Transmittance

f(x) = 91.7821856086 exp( -0.2098775548 x )

Calibration Curve: Concentration vs. Transmittance

f(x) = 91.7821856086 exp( -0.2098775548 x )

bance

ance

ance