Dng 1: PHN NG TO PEPTIT

1. Phn ng to peptit : - Aminoaxit n no c CTTQ : CnH2n +1NO2

- Phn ng to ipeptit : cha 2 gc amnoaxit, khi to ipeptit loi 1 phn t H2O

2CnH2n+1NO2 C2nH4nN2O3 + H2O
- Phn ng to Tripeptit : cha 3 gc amnoaxit, khi to ipeptit loi 2 phn t H2O
3CnH2n+1NO2 C3nH6n 1 N3O4 + 2H2O
- Phn ng to Polipeptit : cha m gc amnoaxit, khi to ipeptit loi (m-1) phn t H 2O
mCnH2n+1NO2 Cm.nH2m.n m +2 NmOm+1 + (m-1)H2O
- nh lut BTKL suy ra : mAminoaxit = mpeptit + mnc
2. Cng thc tnh ng phn pepit
Peptit c n gc - Aminoaxit s c n ! ng phn
V d 1 : Hn hp X cha 0,2mol Glyxin v 0,1 mol Alanin. Khi lng ipeptit ti a to thnh l
A. 27.72 B. 22,7 C.
22,1 D. 21,2
Li gii

Ta c
Ap dng LBTKL suy ra mpeptit = 0,2.75 + 0,1.89 0,15.18 = 21,2 gam chn D
V d 2 : Aminoaxit n chc X cha 15,73%N v khi lng. X to Octapeptit Y. Y c phn t
khi l bao nhiu?
A. 586 B. 771 C. 568 D.
686
Li gii
t X : 2CnH2n+1NO2 C2nH4nN2O3 + H2O

Vy MY = 8.89 7.18 = 586 vc chn A

V d 3 ( B 2010):ipeptit X mch h v Tripeptit Y mch h u to nn t mt aminoaxit
(no, mch h, trong phn t cha 1 nhom NH2- v 1 nhm COOH). t hon ton 0,1 mol Y thu
c tng khi lng CO2 v H2O bng 54,9 gam. t chy hon ton 0,2 mol X, sn phm thu
c dn qua nc vi trong d thu m gam kt ta. Gi tr m?
A. 45 B.120 C. 30 D. 60
Li gii
V d 4 : Hn hp X ca Glyxin v Alanin. Tng s ipeptit v tripeptit to c t X l
A. 8 B. 10 C. 14 D. 12
Li gii
Tng s ipeptit v tripepptit = 22 + 23 = 12 chn D

Dng 2 : PHN NG THY PHN PEPTIT

Thy phn peptit c n gc - Aminoaxit thu c
S ipeptit ti a l : n 1
S tripeptit ti a : n 2
S tetrepeptit ti a : n 3
Ch :
- Da vo phng trnh thy phn tm mi quan h s mol ca cc cht trong mt phng trnh
phn ng xc nh s mol hoc loi peptit.
- Khi lng mol ca n-peptit = -amino axitn - 18(n-1).
- t chy peptit to ra t -amino axit no, mch h cha mt nhm NH 2 v mt nhm COOH theo
phng trnh tng qut sau:
[CaH2a+1O2N]n(1-n)H2O + (3a.n-1,5n)/2 O2 a.n CO2 +(2a.n-n+2)/2 H2O + n/2 N2
V d 1 : Trch on u ca phn t peptit : Gly-Phe-Val-Glu- Cys-Cys-Ala- Ser-Leu-Tyr-Gln.
Dng enzym Proteaza thy phn on peptit trn thu ti a bao nhiu ipepti
A. 10 B. 9 C.
8 D. 11
Li gii
on peptit trn c 11 gc - Aminoaxit nn to 11 1 = 10 ipeptit chn A
V d 2 : Thy phn hon tan m gam tetrapeptit : Ala-Ala-Ala-Ala (mch h) thu hn hp gm
28,48 gam Alanin, 32 gam Ala-Ala v 27,72 gam Ala-Ala-Ala. Gi tr m l
A. 90,6 B. 111,74 C. 81,54 D.
66,44
Li gii
nAla-Ala-Ala = 0,12 mol
nAla-Ala = 0,2 mol
nAla = 0,32 mol
Ta c m sp = 28,48 + 32 + 27,72 = 88,2 gam loi A,B
Phn ng : Ala-Ala-Ala-Ala + 3H2O 4Ala
x 3x 4x
Ala-Ala-Ala-Ala + H2O 2Ala-Ala
y y 2y
Ala-Ala-Ala-Ala + 2H2O 2Ala + Ala-Ala
z 2z 2z z
Ala-Ala-Ala-Ala + H2O Ala + Ala-Ala-Ala
0,12 0,12 0,12 0,12
Th vi p n C : mnc = 88,2 81,54 = 6,66 suy ra nnc = 0,37

BI TP P DNG
Cu 1. Tripeptit X c cng thc cu to sau: Lys-Gly-Ala. Tnh khi lng mui thu c khi thy
phn hon ton 0,1 mol X trong trong dung dch H 2SO4 long. (Gi s axit ly va ).?
A. 70,2 gam B. 50,6 gam
C. 45,7 gam D. 35,1 gam
Cu 2. un nng 0,1 mol tripeptit X c cu trc l Ala-Gly-Glu trong dung dch NaOH (ly d), sau
phn ng hon ton, tnh khi lng mui thu c?
A. 39,9 gam B. 37,7 gam
C. 35,5 gam D. 33,3 gam
Cu 3. T 3 -amino axit X, Y, Z c th to thnh my tripeptit mch h trong c c X, Y, Z?
A. 5 B. 6 C. 3 D. 4
Cu 4. Cho 1 mol peptit X mch h c phn t khi l 461gam/mol thy phn (xt enzim) thu
c hn hp cc -aminoaxit c tng khi lng l 533 gam/mol. Hy cho bit X thuc loi:
A. hexapeptit B. tetrapeptit
C. pentapeptit D. tripeptit
Cu 5. Thy phn hon ton 1 mol peptit X mch h thu c 2 mol alanin; 1 mol glyxin v 1 mol
valin. X c bao nhiu CTCT?
A. 6 B. 24 C. 8 D. 12
Cu 6.Braikinin c tc dng lm gim huyt p. l mt nonapeptit c cng thc l:
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg
Khi thu phn khng hon ton peptit ny c th thu c bao nhiu tripeptit no c cha
phenylalanin (Phe)?
A. 3 B. 5 C. 6 D. 4
Cu 7. C bao nhiu tripeptit (mch h) khc loi m khi thy phn hon ton u thu c 3
aminoaxit: glyxin, alanin v phenylalanin?
A. 6. B. 9. C. 4. D. 3.
Cu 8. Cho 13,32 gam peptit X do n gc alalin to thnh, thy phn hon ton trong mi trng
axit thu c 16,02 gam alalin duy nht. X thuc loi no?
A.Tripeptit B. Tetrapeptit
C. Hexapeptit D. ipeptit
Cu 9.Khi thy phn hon ton 65 gam mt oligopeptit X thu c 22,25 gam alalin v 56,25 gam
glyxin. X thuc loi no?
A. Tripeptit B. Tetrapeptit
C. Hexapeptit D. ipeptit
Cu 10. thi tuyn sinh i hc Khi B- 2012): un nng m gam hn hp gm a mol tetrapeptit
mch h X v 2a mol tripeptit mch h Y vi 600 ml dung dich NaOH 1M va . Sau khi cc
phn ng kt thc, c cn dung dch thu c 72,48 gam mui khan ca cc amino axit u c
mt nhm -COOH v mt nhm -NH2 trong phn t. Gi tr ca m l:
A. 51,72. B. 54,30. C. 66,00. D. 44,48
P N

1 2 3 4 5 6 7 8 9 10

B A B C D B A C B A