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TECHNICAL SHEET

TS 15325
Rev.3 / 08.02.2012
Page 1 of 2

PLATE COUNT AGAR


Medium for surfaces and air total bacterial count

TYPICAL FORMULA (g/l)


Tryptone 5.0
Glucose 1.0
Yeast Extract 2.5
Agar 15.0
Final pH 7.0 0.2

DESCRIPTION
PLATE COUNT AGAR is a medium suggested by APHA, AOAC, ICMSF, ISO for surfaces and air total count of aerobic bacteria,
prepared according to the formulation reported by ISO 4833.

PRINCIPLE
Tryptone is a product obtained by a controlled enzymatic hydrolysis of casein and contains a mix of peptides and free amino acids.
Glucose is a source of energy. Yeast extract is a source of amino acids and vitamins of group B. Agar is the solidifying agent.

TECHNIQUE
Remove the first wrapper of the packet and the inner one. Remove the cap of the plate and gently push the agar surface on a level
surface to examine for at least 10 seconds. Otherwise the plate can be used for air analysis, sampling, if it is possible, 100-500 l of air
with an air sampler. Cover the plate with the cap. Incubate:
! at 25-30C for 682 h for mesophilic bacteria;
! at 25 1C for minimum 7 days (from 2nd day on control for growth) for yeasts and fungi;
! at 30 1C for 68 4 h in the dark for environmental bacteria.

INTERPRETATION OF RESULTS
Observe for the formation of Units Forming Colonies (UFC) and count them referring to the volume of sample analysed.

STORAGE AND TRANSPORT CONDITIONS


10-25C away from light, until the expiry date on the label. Storage or transport at 2-10C do not alter in any way the performance of the
product . Eliminate if signs of deterioration or contamination are evident.

WARNING AND PRECAUTIONS


The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is not
classified as dangerous. It is nevertheless recommended to consult the safety data sheet for its correct use. The product is designed for
professional use and must be used only by properly trained operators.

DISPOSAL OF WASTE
Disposal of waste must be carried out according to the national and local regulations in force.

REFERENCES
1. APHA (1985). Standard Methods for the Examination of Water and Wastewater, 16th edition.
2. ISO 4833: 2003. Microbiology- General Guidance for the enumeration of micro-organisms. Colony count techncique at 30C.
3. ISO 18593. Microbiology of food and animal feeding stuffs- Horizontal method for sampling techniques from surfaces using contact
plates and swabs.

LIOFILCHEM S.r.l.
Via Scozia, Zona Ind.le - 64026, Roseto degli Abruzzi (TE) - ITALY
Tel +39 0858930745 Fax +39 0858930330 Website: www.liofilchem.net E-mail: liofilchem@liofilchem.net
TECHNICAL SHEET
TS 15325
Rev.3 / 08.02.2012
Page 2 of 2

PRODUCT SPECIFICATION
NAME
PLATE COUNT AGAR

PRESENTATION
Rodac plates containing 171 ml of medium

STORAGE
10-25C

PACKAGING
Ref. Content Packaging
15325 20 plates ! Plates individually packed in blister
! Carboard box
! Outside label

pH OF THE MEDIUM
7.0 0.2

USE
PLATE COUNT AGAR is a medium suggested by APHA, AOAC, ICMSF, ISO for surfaces and air total count of aerobic bacteria,
prepared according to the formulation reported by ISO 4833

TECHNIQUE
Refer to technical sheet of the product

APPEARANCE OF THE MEDIUM


Light amber medium, slightly opalescent

SHELFLIFE
9 monts

QUALITY CONTROL
1. Control of general characteristics, label and print
2. Sterility control
7 days at 22 1C, in aerobiosis
7 days at 36 1C, in aerobiosis
3. Microbiological control
Inoculum for productivity: 10-100 CFU/ml
Incubation Conditions: 18-24 h at 36 1C, in aerobiosis

Microorganisms Growth
Bacillus subtilis ATCC 6633 Good
Staphylococcus aureus ATCC 25923 Good
Escherichia coli ATCC 25922 Good

TABLE OF SYMBOLS
Batch Fragile, handle with
Do not reuse Manufacturer Use by
code care

Catalogue Temperature Contains sufficient Caution, consult


number limitation for <n> tests instructions for use

LIOFILCHEM S.r.l.
Via Scozia, Zona Ind.le - 64026, Roseto degli Abruzzi (TE) - ITALY
Tel +39 0858930745 Fax +39 0858930330 Website: www.liofilchem.net E-mail: liofilchem@liofilchem.net

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