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OF AN AMINO ACID
Experiment 2
Ebru AKHARMAN, Gebze Technical University, Turkey
AIM:
1. To determine the titration curve for an amino acid.
2. To use this curve to estimate the values of the ionizable groups of the amino acid.
In this experiment, unknown amino acid is identified via an acid base titration.
[ ]
= + log
[]
= []. Thus,
[ ]
=1
[]
log 1 = 0
=
1 + 2
=
2
2,61 + 10,34
=
2
= 6, 48
DISCUSSION: RESOURCES:
When testing, it is important to know the http://vlab.amrita.edu/?sub=3&brch=63&si
amount of test material. In this experiment, m=1336&cnt=1
the pKa of an unknown amino acid was
calculated and the identity of this amino https://www.chem.fsu.edu/chemlab/bch40
acid was estimated. Before describing this 53l/Protein%20Characterization/AA%20Ti
process, I would like to talk about the tration/index.html
properties of amino acidsAll of the amino
acids have an acidic group (COOH) and a
basic group (2 ) attached to the carbon,
and also they contain ionizable groups that
act as weak acids or bases, giving off or
taking an protons when the pH is altered.
The pH at which the net charge of an amino
acid is zero is called the isoelectric point, or
the pI. The isoelectric point is always half
way between the values.The amino
acid is estimated by utilizing the pI value. In
this experiment NaOH is added until the
unknown amino acid is pH 11. After each
addition of NaOH, the pH is measured and
the data recorded. Graphics are generated
from the obtained data. The pi is then
calculated using the Formula:
+ +
=