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Introduction
In this experiment, the calculated mass and actual mass was used to calculate the
concentration of a particular chemical substance. By this, the knowledge taught in class was
used to investigate the concentration of an unknown solution by using the calibration curve.
Also, this knowledge could be developed and used in future study: forensic science.
Materials
Procedure
1. Wear labcoat, tie your hair
2. Put on gloves
3. Prepare materials : spatula, massing paper, balance spectrophotometer CUSO4
(copper sulfate) and H2O (water) , volumetric flask
4. Place the massing paper on the balance and press tear
5. Use the spatula to put CUSO4 on to the balance and weight them (after weighing all
of the 4 masses we need)
6. Dissolve CUSO4 in a beaker then transfer the substance into volumetric flask/ adjust
volume to 100.00ml
7. Dissolce the CUSO4 and H2O in the spectophometer
8. Dissolve the response - absorbance at 810nm (3 trails)
9. Place the data into excel sheet
10. Create the graph in excel with standard calibration curve
Data
The equation that we use to calculate for the concentration is
T heorical concentration(M )
249.68 g/mol(molecular weight)0.1L
For the absorbance, we averaged the absorbance of 3 trials
T rial 1 result + T rial 2 result + T rial 3 result
3
Report the value of slope, y-intercept, and R2
=slope(array of y- absorbance, array of x- concentration)
However, the absorbance of a greater concentration should be tested in order to make good
prediction of a higher amount of concentration.
Conclusion
Overall, the relation of the concentration and absorbance is directly proportional. Since our
standard calibration curve is quite accurate, the equation that was discovered in this
experiment could be used to make future assumption of the absorbance produced after
increasing the concentration of the solution.
Error
The distilled water mixing with copper sulfate has been kept in the beaker for too long, this
might affect the accuracy of the numbers according to the fading intensity of the substance.
Moreover, another error is that the distilled water has been pour over a line marked on a
cuvette, the accurateness of the light passing through the cuvette will be decreased which
will produce inaccurate numbers.