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TEKNOLOGI BIOPROSES

Bioreactor Design
Nur Istianah, ST.,MT.,M.Eng
Bioreactor

Nur Istianah-THP-FTP-UB-2017
http://upload.wikimedia.org/wikipedia/commons/7/71/Bioreactor_principle_svgedit.
Project!
An industry need to produce 100
ton per day of bioethanol from
cassava tubers. It is suplaid by
2000 ton cassava tubers per day.
Could you tell us how about the
spesification of bioreactor should
we use?
Nur Istianah-THP-FTP-UB-2017
Design, calcutate, discuss
Designing steps

Bioreactor • Mass Dimension


• Require types Transfer
• Power
ments • Kinetics • Heat consumption
Transfer
• Hold Up
Microbes Dynamics
Bioreactor Operation Modes

FED PLUG
BATCH BATCH CSTR
FLOW

http://www.aspentech.com

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Nur Istianah-THP-FTP-UB-2017
-1.a. Batch Operation
•A foam breaker may be installed to disperse foam
A batch bioreactor is
normally equipped
with an agitator to
mix the reactant, and dCs rmax CS
r 
the pH of the dt K m  CS
reactant is
maintained by
employing either
buffer solution or a Batch operation
with stirring
pH controller

 Cs 0  Cs   rmax t
Cs 0
Change of Cs
K m ln
with time, t
Cs
Nur Istianah-THP-FTP-UB-2017
Case study
• Contoh soal 1:
• Sebuah bioreaktor digunakan untuk
memproduksi enzim dengan konsentrasi
substrat awal sebesar 12x10-3 M dan
konversi bioreaktor 90%. Berapakah
waktu yang diperlukan untuk produksi
enzim jika data kinetik reaktor diberikan
sebagai berikut:
• rmax = 2.5 mmol/m3.det; Km = 8.9x10-3 M
Solution
• Konsentrasi substrat akhir,
• Cs = (1-XA)Cs0 = (1-0.9)( 12x10-3 M) = 1.2x10-3 M
• rmax = 2.5 mmol/m3det= 2.5 x10-6 mol/L.det

1  Cs0 
t  K m ln  C s 0  C s 
rmax  Cs 
 
 
-3
1 12 x10
t  8.9x10 -3 ln  12 x10 -3
 1.2 x10 -3

2.5x10 -6  1.2 x10 -3

t  12517 det  3.5 jam
Case study
• Contoh soal 2:
• Hitunglah laju fermentasi etanol dari nira
sorgum jika konsentrasi gula awal sebesar
0.1 M dan di akhir fermentasi diperoleh
konsentrasi gula sebesar 0.025 M.
Fermentasi berlangsung selama 4 jam
dengan konstanta Monod 5x10-3 M.
Solution
1 Cs0 
rmax 
  K m ln  C s 0  C s 
t Cs 
1 
 0.1  0.025
0.1
rmax   5x10 ln
-3

4 0.025 
rmax  0.02M . jam 1
rmax C S
r
K m  CS
0.02(0.025)
r
5x10 -3  0.025
1
r  0.017 M . jam
• Laju fermentasi bioetanol tersebut adalah 0.017 M.jam-1
-1.b. Fed Batch Operation

Nur Istianah-THP-FTP-UB-2017
Case study
• Contoh soal 3:
• Sebuah bioreaktor fed batch dioperasikan pada
volume cairan awal 600 mL. Untuk menjaga
kebutuhan nutrisi mikroba didalamnya, telah
dialirkan tambahan media dengan laju alir 0.01
mL/det. Tentukan volume akhir cairan selama
proses fermentasi 2 jam dan tentukan pula
konsentrasi biomas jika pada akhir fermentasi
diperoleh 3.5gr padatan kering(biomass).
Diasumsikan berat molekul biomass sebesar
367 gr/mol.
Solution
dV
 FA
dt
dV  FA dt
Vt  V0  FA t
Vt  V0  FA t
mL
Vt  600mL  0.01 x 2 x3600 det
det
Vt  672mL 3.5 gr
1
367 gr.mol
Konsentrasi biomass (X) = X 
0.672 L
X  0.14M
-2. Plug-flow mode
An ideal plug-flow reactor can
approximate the long tube,
In a plug-flow packed-bed and hollow fiber or
reactor, the substrate multistaged reactor
enters one end of a
cylindrical tube with F, Cs0 V F, Cs
is packed with
immobilized enzyme V Residence
and the product t=0  time
steam leaves at the F
Continuous
other end. operation without
stirring

Cs0
K m ln  C s 0  C s   rmax
Cs
Nur Istianah-THP-FTP-UB-2017 11
THANKS FOR YOUR ATTENTION

The best person is one give something useful always

Nur Istianah-THP-FTP-UB-2017

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