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available to today’s scientists. One of the great advantages of infrared spectroscopy is that
virtually any sample in virtually any state may be studied. Infrared spectrometers have been
commercially available since the 1940s. At that time, the instruments relied on prisms to act as
dispersive elements, but by the mid 1950s, diffraction gratings had been introduced into
dispersive machines. The most significant advances in infrared spectroscopy have come about
as a result of the introduction of Fourier-transform spectrometers.
Figure 15.2 Major vibrational modes for a nonlinear group, CH2. (+ indicates motion from the plane of page toward
reader; – indicates motion from the plane of page away from reader.) (Reprinted from R. M. Silverstein,
G. C. Bassler, and T. C. Morrill, Spectrometric Identification of Organic Compounds, 4th edition. New York: JohnWiley & Sons, 1981, p. 166, by permission of John
Wiley & Sons, Inc., copyright © 1981.)
Principles of IR Spectroscopy
The IR region of the EM spectrum
Infrared radiation is electromagnetic energy with wavelengths longer than visible light but
shorter than microwaves. Generally, wavelengths from 0.8 to 100 micrometers can be used for
IR spectroscopy and are divided into the near-IR (0.8-2.5 μm), the mid-IR (2.5-15 μm) and the
far-IR (15-100 μm) regions. The near and mid IR regions are most useful for quantitative and
qualititative analysis of foods.
IR radiation also can be measured in terms of its frequency, which is useful because
frequency is directly related to the energy of the radiation by the following relationship: E=hv
Where E=energy of the system h=Planck’s constant v=frequency in hertz
Molecular vibrations
A molecule can absorb IR radiation if it vibrates in such a way that its charge distribution
and therefore its electric dipole moment changes during the vibration. The most important
vibrations that produce a change in dipole moment are stretching and bending (scissoring,
rocking, twisting, wagging motions). Stretching motions vibrate at higher frequencies than
scissoring motions. Also, asymmetric stretches are more likely to result in a change in dipole
moment, with corresponding absorption of IR radiation than asymmetric stretches.