Group No.

69 Date Performed: February 12,

2010
Chemistry Laboratory 204B
Members:
1. Amlih, Abdulrahman B. – Principal Author
2. Enriquez, Wilson T.
3. Jalani, Zulaykha Zahra B.

Laboratory Report Sheet

Protein and Amino Acids II: Activity
Precipitation 12

Objective(s): 1.
_________________________________________________________
2. _________________________________________________________
3.
_________________________________________________________
4. _________________________________________________________

Data Interpretation:

Name of Test Reagents No. of Effect of Excess

Test Solutions Added Drops Reagent
Added to
Produce
Precipitat
e

A. Salts of 1% 1% 13 drops Dissolved

Heavy Albumin Pb(CH3COO
Metals )2

1% HgCl2 15 drops Dissolved

1% 15 drops Dissolved
Cd(NO3)2

B. Alkaloidal 1% Picric acid 4 drops Dissolved

Reagents Albumin

10% Tannic 4 drops Increased Precipitate

acid

10% TCA 4 drops Dissolved

20% N/A

Natural Sciences Department, College of Science and Information Technology, Ateneo de Zamboanga
University
La Purisima Street, Zamboanga City, Philippines
 Tungstic
acid

Name of Test Reagents Result Explanation

Test Solutions Added
C. Inorganic 1% conc. HCl No
Acids Filtered precipitate
Albumin formed

conc. H2SO4 No
Precipitate
formed

conc. HNO3 No
Precipitate
Formed

D. Heller’s O.5% conc. HNO3 Formed

Test Albumin white
Precipitate

0.25% Formed
Albumin (slightly)
White
precipitate
0.12% No white
Albumin precipitate
formed

Name of Test Reagents Result Solubility of

Test Solutions Added Precipitate in
Water
E. Alcohol 1% 95% No
Albumin alcohol precipitate,
and vague
white
solution

1% Gelatin White Soluble

solution
with white
precipitate
formed

1% No
Peptone precipitate
with cloudy

Natural Sciences Department, College of Science and Information Technology, Ateneo de Zamboanga
University
La Purisima Street, Zamboanga City, Philippines
 solution

1% 70% Formed Soluble

Albumin alcohol white
precipitate

Name of Test Reagents Result with Explanation

Test System Used Biuret Test
in
F. Heat test tube Powdered Greater
1 Egg amount of
(mixed, Albumin precipitate
heated, and retained,
then Distilled formed violet
filtered) Water junction

test tube Powdered Lesser

2 Egg amount of
(heated, Albumin precipitate
mixed, and remained,
then Distilled turned violet
filtered) Water color

Questions:
(Answers to questions included in the procedure. Be brief and concise.
Quotes from books or internet sites should be given proper credit – use
footnotes for references. The only General Academic Policy is HONESTY.)

1. What is denaturation?
2. Why is egg white used as an antidote for lead or mercury poisoning?
3. Explain the use of tannic acid and picric acid in the treatment of burns.
4. Can heat coagulation test be used to detect the presence of albumin in
urine? Explain.
5. Which is more effective as a disinfectant – 95% alcohol or 70% alcohol?
Why?

ADDITIONAL NOTE ON QUESTIONS: Please be sure to answer the question

accurately. Copying-pasting from net references is highly discouraged. Draw
out the necessary ideas but DO NOT INCLUDE non-essential statements. If
sourcing from the net is done, please be sure to accurately reflect the
sources in the reference part).

Conclusion/Generalization:
Natural Sciences Department, College of Science and Information Technology, Ateneo de Zamboanga
University
La Purisima Street, Zamboanga City, Philippines
(General statement about the observations made/principles involved. Be brief
and concise. Minimum of 3 statements, maximum of 10. AVOID using phrases
like “We therefore conclude…”, “we…”, “our group…” statements, and the
like. These are non-essential phrases. If performers really need to be
mentioned, state in the third person, e.g. “The members of the group…”)

References:
(Cite used references here. Use standard format as taught in English classes,
especially proper format for references taken from the internet.)
NOTE ON FORMAT CHANGES: Please use this format for the laboratory
report strictly. Simply save a soft copy of this sample, and edit to prepare the
report. Font type – Tahoma regular; size – 11.

Natural Sciences Department, College of Science and Information Technology, Ateneo de Zamboanga
University
La Purisima Street, Zamboanga City, Philippines