Top 5 Antibody Humanization Service Providers in the Antibody Market

Top 1 GenScript

Founded in 2002 in New Jersey, United States, GenScript serves as a partner for researchers in
basic life sciences, translational and biomedical fields as well as early-stage drug development.

As a one-stop supplier for therapeutic antibody discovery and development, GenScript is proud to
offer integrated antibody humanization and affinity maturation service. Combining in silico
CDR-grafting, structure-based back mutation, and, supported by our patented FASEBA(Fast
Screening for Expression, Biophysical-properties and Affinity) screening, Biacore Surface
Plasmon Resonance platform and functional assay platform, the antibody humanization service is
offered at GenScript to generate humanized antibody maintaining the affinity and specificity of
parental antibody and optimized activity, productivity and thermostability. Seamlessly integrated
with humanization, the affinity maturation service will continuously improve the affinity by 10 to
50 folds.

Top 2 Sino Biological

Founded by MIT professor, Dr. Daniel I.C. Wang, and his former student, Dr. Liangzhi Xie, in
2007, Sino Biological Inc. is a developing biological reagents manufacturer located in Beijing,
offering a comprehensive set of premium quality reagents, all of which are produced in-house and
cover a broad range of life science research and drug development.

Sino Biological's antibody humanization experience covers not only mouse antibody but also
rabbit monoclonal antibody. Our record speed for antibody humanization was 2 weeks, including
antibody molecule structure modification, high-throughput antibody production by transient
transfection, antibody characterization and bioactivity measurement.

Top 3 Creative Biolabs

Since its establishment in 2005 in New York, Creative Biolabs is specialized in providing custom
biotechnology and pharmaceutical services that cover the full scope of biotechnology needs of
early antibody development and drug discovery. It has extensive experience to offer the antibody
humanization service for therapeutic and diagnostic development. It has successfully performed
15 mouse/rat humanization projects during the past decade with at least one humanized antibody
entered the clinical trials. It also provides humanization service to antibodies derived from other
species, such as non-human primate (NHP), rabbit, dog, chicken, llama and etc.

As a means of circumventing common problems, Creative Biolabs has developed three antibody
humanization strategies that can preserve the specificity and affinity of the antibody toward the
antigen whereas significantly or completely eliminate the immunogenicity of the antibody in
humans. The first approach is CDR grafting and the second is chain shuffling. These two methods
are all based on phage display of humanized scFv variants and selection of high-affinity
humanized binders through bio-panning. The third method, humanized IgG library screening, is
somehow unique. It will make a library of humanized whole IgG to be displayed on the surface of
mammalian cells and then high affinity binders will be sorted by FACS.

Top 4 Fusion Antibodies

Fusion Antibodies is a Contract Research Organisation (CRO) established in 2001 and located
about 6 miles outside Belfast city centre in Northern Ireland, UK. Its mission is to enable
biopharmaceutical and diagnostic companies to develop innovative products in a timely and
cost-effective manner for the benefit of the global healthcare industry.

Built on decades of combined experience in the antibody development industry, its CDRxTM
platform enables a robust, reliable and accurate modernized version of the CDR grafting technique.
It identifies and combines the essential sequences from antibody variable domains into human
donor sequences to create a panel of full length humanized antibodies for expression. The Fusion
Antibodies humanization platform produces antibodies which are compliant with the WHO/INN
definitions of a humanized antibody to receive the -zu- species origin substem.

Top 5 Absolute Antibody

Absolute Antibody was founded in Oxford, UK, in August 2012, with the vision that all antibody
users should be able to use recombinant antibodies, which are absolutely defined by their amino
acid sequence. Rather than creating entirely new antibodies (or antibody-like molecules), it is
building a catalog by taking existing antibodies (often from hybridomas) then manufacturing them
in a superior way and improving their usefulness by engineering.

Absolute Antibody delivers a panel of humanized variants from your clone, and guarantee to
produce a variant with comparable activity to the parent. Its process also identifies variants with
preferential manufacturing criteria (e.g. high expression, low aggregation).

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