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1515/agr-2016-0013
Abstract
The aim of the study is to try a systematic and rational exploration of regeneration potential of extinct taxons, starting from
old seeds found in the soil or in the herbarium, to study their germination potential with the purpose of biodiversity
conservation. The species involved in this study are: Achillea spinosa, Chenopodium wolfii. Several protocols for the in
vitro propagation of the species, starting from the seed are described in this study. The presence of callus during
micropropagation of plants has also been the object of research for different compositions of nutritive medium.
1. INTRODUCTION
The IUCN Red List of Threatened Species provides of 5 years. The age of seed is over 100 years old. So,
taxonomic, conservation status and distribution we try to see if the germination capacity is
information on plants, fungi and animals that have conserved, despite the age and the conservation
been globally evaluated using the IUCN Red List method.
Categories and Criteria.
This system is designed to determine the relative
risk of extinction, and the main purpose of the IUCN
Red List is to catalogue and highlight those plants
and animals that are facing a higher risk of global
extinction (i.e. those listed as Critically
Endangered, Endangered and Vulnerable). The
IUCN Red List also includes information on plants,
fungi and animals that are categorized
as Extinct or Extinct in the Wild.
The IUCN Red List Index measures overall trends
in extinction risk for groups of species based on
genuine changes in their Red List status over time.
The aims of this study is to find Balkan endemic
extinct seed species on their old natural stations or
in ancient herbals and study their germination
potential.
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THE ANNALS OF “VALAHIA” UNIVERSITY OF TARGOVISTE
2016
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THE ANNALS OF “VALAHIA” UNIVERSITY OF TARGOVISTE
2016
2. wash in double distilled sterile water and In vitro technology of Achillea millefolium in the
maintain in water for 20 minutes; images (fig.7-10).
sterilization in ethanol 700 for 5 minutes
sterilization in Ca hypochlorite 6% for 30
minutes and rinse 3 times with sterile
double distilled water and maintain in
water until prelevment.
The best results were obtained on the first variant.
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THE ANNALS OF “VALAHIA” UNIVERSITY OF TARGOVISTE
2016
For Achillea spinosa, we continue the study of [14] Saeidnia S, Gohari A, Mokhber-Dezfuli N, Kiuchi
regeneration capacity by in vitro culture, despite of F. A review on phytochemistry and medicinal
advanced age. The seeds found in the soil showed a properties of the genus Achillea. Daru 2011;19:173-
86
good conservation status.
[15] Sharifi-Rad J, Mnayer D, Roointan A, Shahri F,
Ayatollahi SA, Sharifi-Rad M, Molaee N, Sharifi-
5. ACKNOWLEDGEMENTS Rad M, 2016. Antibacterial activities of essential
oils from Iranian medicinal plants on extended-
This research was realized with the Research Institut spectrum β-lactamase-producing Escherichia
for Pomology Pitesti-Maracineni (Roumanie) coli. Cellular and molecular biology, Volume: 62
support. Issue: 9 Pages: 75-82
[16] Shibli, R. A., Shatnawi, M. A., Subaih, W. and
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